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Biological Control of Wood Destroying Organism Using Plant Extracts Collected from Mt. Merapi National Park, Indonesia Ismayati, Maya; Zulfiana, Deni; Tarmadi, Didi; Lestari, Anis Sri; Krishanti, Ni Putu Ratna Ayu; Himmi, Setiawan Khoirul; Fajar, Anugerah; Yusuf, Sulaeman
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20102

Abstract

Due to the climate change and global warming, the biodiversity database has gained the attention of the government. In line with the Indonesian Biodiversity Strategy and Action Plan (IBSAP), we have collected plants with insecticidal activity based on the local wisdom. This program aimed to protect Indonesian biodiversity from deforestation along with the loss of the number of species. This research?s goal was to evaluate termiticidal and antifungal properties from some plant extracts collected from the Turgo forest area, Mt. Merapi National park, Java. Three potential plants were evaluated. Based on specimen identification, the three plant samples were Kina (Cinchona sp.), Kamadoh (Dendrocnide stimulans (L.f.) Chew), and Keremi (Homalanthus populneus (Geiseler) Pax). The phytochemical test showed that Kina contained alkoloid, falvonoid, saponin, and tannin, whereas Kamadoh and Keremi contained saponin and tanin, respectively. Overall, all plant extracts have the termiticidal activities and able to inhibit wood- decay fungi with the inhibition percentage around 60% - 100%. Leaf extracts of Kina (Cinchona sp) and Keremi (Homalanthus populneus (Geiseler) showed the highest activity as wood-decay fungi inhibitor. The disclosure of the potential of bioinsecticides from some plants originating from Mount Merapi is very important before being lost due to deforestation and the Mount Merapi disaster. Thus, the potential bioinsecticide in these plants can increase its economic value as a substitute for synthetic insecticides that are friendly to the environment.
Application of LC-MS/MS Coupled with Various Digestion Methods for the Identification of Porcine Gelatin Markers in Confectionery Matrices Dewi, Kifayati Rosiyanti; Kusnandar, Feri; Yuliana, Nancy Dewi; Ismayati, Maya; Solihat, Nissa Nurfajrin; Riantana, Handy; Heryani, Heryani
Indonesian Journal of Halal Research Vol 5, No 2 (2023): August
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v5i2.21191

Abstract

Gelatin is a high-risk ingredient in terms of its halal status. Liquid chromatography combined with mass spectrometry (LC-MS/MS) was used to identify the source of gelatin based on marker peptides and proved to deliver higher reliability than other methods. However, the digestion method is essential before LC-MS/MS analysis. This research evaluated different digestion methods against selected porcine gelatin marker peptides and assessed LC-MS/MS sensitivity through adulteration experiments in various mixed matrices. The study involved three digestion methods (conventional, microwave, and ultrasound) before LC-MS/MS analysis to determine the most effective method for detecting marker peptide targets from porcine gelatin. The appropriate method was applied to isolate porcine gelatin peptides in the matrices of bovine gelatin and confectionery products (lozenges, marshmallows, and soft candy) at concentrations of 0.01, 0.1, and 1% (w/w). Relative detection limit values were determined. The results showed that conventional digestion treatment yielded a higher marker peptide detection rate than microwave and ultrasound digestion. Meanwhile, the detection limit of porcine gelatin in bovine gelatin ranged from 0.09 to 0.89%, depending on the marker peptide used, and could be significantly detected at a concentration of 1% in the confectionery product. The marker peptide TGQPGAVGPAGIR exhibited the highest stability, as it was detectable at the lowest concentration across all mixed matrices. The LC-MS/MS method has been proven to afford sensitive results and has the potential to serve as an alternative for detection of halal status.
Utilization of Tannins with Various Polymers for Green-Based Active Packaging: A Review Agrippina, Fidela Devina; Ismayati, Maya; Hidayati, Sri; Pratama, Bima Putra
Jurnal Sylva Lestari Vol. 12 No. 3 (2024): September
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl.v12i3.893

Abstract

Tannin is a major polyphenolic secondary metabolite widely distributed in the forestry sector and can be added to polymer matrices to manufacture bioactive films for food packaging. Tannins have functional properties as antioxidants, antibacterial, and anti-inflammatories. Tannins are classified into two types, namely condensed tannins and hydrolyzable tannins. Tannins have been used primarily in food, wood, leather, pharmaceutical, and other industries. In the food industry, tannins are used to develop food packaging, preservation, and the function of the food industry. Tannin molecules have the advantage of combining with polymers or polymer surfaces, for example, synthetic polymers, biopolymers, and micro- and nano-sized fibers, which will form new products whose physical and chemical properties increase in functional properties because of the presence of hydrophilic and nucleophilic groups. Combining tannins with a polymer can be done by one-step reactive extrusion, layer by layer, dynamic vulcanization, acetylation, and in situ extraction methods. This paper describes general information about tannins, followed by applications using tannins, tannin-based hybrid materials, and methods of combining tannins with a polymer for food packaging purposes. Keywords: active packaging, bioactive film, polymer, secondary metabolite, tannins