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Acta Biochimica Indonesiana
Published by Perhimpunan Biokimia dan Biologi Molekuler Indonesia
ISSN : 26546108     EISSN : 26543222     DOI : https://doi.org/10.32889
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Acta Biochimica Indonesiana (ActaBiolna) is a peer-reviewed and open-access journal that disseminates original research articles and review articles covering diverse topics in Biochemistry and Molecular Biology. The journal is published biannually by Indonesian Society for Biochemistry and Molecular Biology.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 6 Documents
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Search results for , issue "Vol. 5 No. 2 (2022): Acta Biochimica Indonesiana" : 6 Documents clear
The impact of fasting toward oxidative stress marker in the liver and plasma of new zealand white rabbit Novi Silvia Hardiany; Stephanie Gosal; Damayanti Angelina; Engelbert Julyan Gravianto; Radiana Dhewayani Antarianto
Acta Biochimica Indonesiana Vol. 5 No. 2 (2022): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.112

Abstract

Background: Fasting may increase the activity of endogenous antioxidants and protect against oxidative stress. However, the effects of different fasting durations on the liver have not been reported. Objective: The purpose of this study is to determine the effect of intermittent and prolonged fasting on oxidative stress markers in the liver tissue and plasma of New Zealand White rabbits. Methods: New Zealand White rabbits were divided into three groups: control, intermittent fasting (IF), and prolonged fasting (PF), with each group consisting of five rabbits. The control group was provided with food ad libitum; the IF group fasted for 16 hours, while the PF group fasted for 40 hours, followed by an eight-hour non-fasting period for six days. In liver tissue and plasma, oxidative stress indicators (catalase, carbonyl, GSH) were evaluated. Results: In the IF group, liver GSH was significantly higher than in the control group. However, neither liver carbonyl nor catalase levels changed significantly in the IF group. In the IF group, plasma carbonyl was significantly lower than in the PF group. In addition, there was no significant differences between groups in plasma catalase and GSH levels. Conclusion: Intermittent fasting and prolonged fasting could significantly increase liver GSH levels of New Zealand White rabbits. In addition, intermittent fasting is more effective than prolonged fasting at preventing oxidative stress.
Malondialdehyde and carbonyl levels in skeletal muscle tissues after intermittent hypobaric hypoxia exposures Syarifah Dewi; Alexander Rafael Satyadharma; Albertus Raditya Danendra; Wardaya
Acta Biochimica Indonesiana Vol. 5 No. 2 (2022): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.113

Abstract

Background: Hypobaric hypoxia is a state of decreased oxygen pressure at high altitudes that can lead to hypoxia and oxidative stress as a result. Skeletal muscle is one of the important organs that can be affected by oxidative stress and cause contractile dysfunction. Objective: This study aimed to evaluate the impact of intermittent hypobaric hypoxia on oxidative stress markers in rat skeletal muscle, by measuring malondialdehyde (MDA) and carbonyl levels. Methods: Twenty-five Wistar rats were allocated into five groups, including one control group and four hypoxic groups (I-IV). The hypoxic groups were exposed to an altitude of 25,000 feet for 5 minutes using hypobaric chamber in once (I), twice (II), three (III), and four (IV) times, with a 7-day interval period between exposures. The control group remained in normobaric conditions throughout the study. MDA levels were measured by thiobarbituric acid (TBA) test, while carbonyl levels were measured using 2,4-dinitrophenylhydrazine (DNPH) reagent. Results: The MDA level was significantly increased in group I compared to the control group (p=0.008). There were decreasing MDA levels in groups II, III, and IV compared to group I. The carbonyl level was significantly higher in group I than the control group (p=0.000), with an even higher level observed in group II. Although the carbonyl levels tended to decrease in groups III and IV, they still remained higher than those of the control group. Conclusion: Exposure to hypobaric hypoxia leads to an increase in MDA and carbonyl levels in the skeletal muscles, indicating an elevation of oxidative stress levels. However, the subsequent intermittent hypobaric hypoxia exposure resulted in a reduction in these levels, implying that skeletal muscles may adapt to hypoxic conditions.
Is heme biosynthesis influenced the mitochondrial function and cell proliferation in cancer? Raisa Nauli; Sri Widia A Jusman
Acta Biochimica Indonesiana Vol. 5 No. 2 (2022): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.65

Abstract

Heme is a compound consisting of an iron (Fe) atom bound to a pyrrole ring forming protoporphyrin IX (PPIX). Protoporphyrin combines with a protein-forming hemoprotein compound that plays an essential role in oxygen-binding and transport as well as in the process of energy production in the mitochondria. Some cancer cells have more heme biosynthesis than normal cells, which is thought to be linked to more cancer cell growth. Inhibition of heme biosynthesis in some cancer cells leads to decreased cell proliferation. This review article discusses the synthesis of heme, the role of heme in energy metabolism, which is needed for cell proliferation, the inhibition of heme synthesis and its effect on cancer cell proliferation, and the possibility of the inhibition of heme biosynthesis as an approach in therapy of cancer in the future.
Estimation of malondialdehyde and catalase activity in pregnant women at IIMS&R Hospital, Lucknow, India Niharika Singh; Saba Khan; Mohammad Mustufa Khan; Haseeb Ahsan; Roshan Alam
Acta Biochimica Indonesiana Vol. 5 No. 2 (2022): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.89

Abstract

Background: During pregnancy, the physiological production of reactive oxygen species (ROS) is associated with a variety of maternal, placental, and fetal developmental functions. These functions are disrupted by excessive amounts of ROS, resulting to pregnancy complications. Different stages of pregnancy require a balance between oxidant and antioxidant production. Objective: The aim of this study is to investigate the status of malondialdehyde (MDA) and catalase (CAT) activity in pregnant and age matched non-pregnant women. Methods: In this cross-sectional study, a total of 74 participants were enrolled, including 37 pregnant and 37 age-matched non-pregnant women. The age range of participants was 18 to 40 years. MDA and CAT levels were measured spectrophotometrically. A p-value 0.05 was statistically significant. Results: Compared to non-pregnant women, the mean plasma level of MDA was considerably elevated in pregnant women (p<0.0001). However, the mean level of CAT activity in pregnant women was significantly lower than in non-pregnant women (p<0.0001) In pregnant women, there is a significant strong negative correlation between MDA levels and CAT activity (p<0.01). Conclusion: Pregnant women have high level of oxidative stress, indicating that the pregnant women are more susceptible to oxidative damage and may develop pregnancy-associated complications.
Jamblang (Syzygium cumini) leaf extract decreased hydrogen peroxide in lead acetate-induced rats Rauza Sukma Rita; Elmatris Sy; Endrinaldi
Acta Biochimica Indonesiana Vol. 5 No. 2 (2022): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.118

Abstract

Background: Free radicals are atoms or molecules with one or more unpaired electrons. Lead acetate has been reported to increase the presence of free radicals in the body. Objective: This study aims to investigate the effect of oral administration of jamblang (Syzygium cumini) leaf extract on hydrogen peroxide (H2O2) serum levels in rats induced with lead acetate. Methods: A total of 24 male rats were divided into three groups: the negative control, the positive control, and the treatment group. The negative control group received a standard diet, the positive control group received lead acetate at a dose of 40 mg/kg body weight, and the treatment group received lead acetate at the same dose along with jamblang leaf extract at a dose of 150 mg/kg body weight. Lead acetate and jamblang leaf extract administration was carried out for 30 days. Afterward, serum H2O2 levels were examined using the colorimetry method. Results: Results revealed that H2O2 levels in the negative control, positive control, and treatment groups were 3.08±0.24, 4.94±0.75, and 3.44±0.65 nmol/L, respectively. Significant differences were observed between the groups, as well as between the negative control and positive control, and between the positive control and treatment group. Conclusion: The study showed that jamblang leaf extract can reduce hydrogen peroxide levels in Wistar rats treated with lead acetate, indicating its ability to address oxidative stress.
Histopathological analysis of the liver in hypercholesterolemia rats treated with Dillenia serrata fruits Tien; Tri Wistya Utami; Pranita Aritrina; Laode Kardin; Sukurni; Andi Noor Kholidha Syarifin
Acta Biochimica Indonesiana Vol. 5 No. 2 (2022): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.56

Abstract

Background: Dillenia serrata (locally known as singi) is a natural product with the potential to improve liver function in hypercholesterolemia, due to anticholesterol and antioxidant properties. Objective: This study aimed to investigate the effect of D. serrata on the histopathological features of rat livers induced by a high-fat diet. Methods: This study used a quasi-experimental in vivo with a post-test-only control group design. Rats were divided into four groups: normal, high-fat diet, as well as D. serrata, and simvastatin treatment. Hypercholesterolemia was induced by a high-fat diet for two weeks. Rat liver tissues were analyzed histologically using Hematoxylin-Eosin (HE) staining, and were observed under a light microscope at 40x magnification in five wide fields of view. Results: The high-fat diet group had the most adipose cells, while the D. serrata group had the least. Statistical analysis demonstrated a significant difference between the high-fat diet group and the other groups (p < 0.05). Interestingly, no significant difference between the D. serrata group and either the normal or simvastatin group (p > 0.05), suggesting the treatment of fruit may restore liver function comparable to normal and simvastatin group. Conclusion: The D. serrata fruit reduces the number of fat cells in the histopathology of rats induced with the high-fat diet.

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