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Acta Biochimica Indonesiana
Published by Perhimpunan Biokimia dan Biologi Molekuler Indonesia
ISSN : 26546108     EISSN : 26543222     DOI : https://doi.org/10.32889
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Acta Biochimica Indonesiana (ActaBiolna) is a peer-reviewed and open-access journal that disseminates original research articles and review articles covering diverse topics in Biochemistry and Molecular Biology. The journal is published biannually by Indonesian Society for Biochemistry and Molecular Biology.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 5 Documents
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Search results for , issue "Vol. 6 No. 1 (2023): Acta Biochimica Indonesiana" : 5 Documents clear
Relative expression of IL-10 and TNF-α mRNA of kidney and spleen tissues of rat with and without mammary tumor after exposed to alternating current electric field Nafisa Khoirun Widiasri; Anysah Nur Fauziyah; Firman Alamsyah; Rarastoeti Pratiwi
Acta Biochimica Indonesiana Vol. 6 No. 1 (2023): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.107

Abstract

Background: Cancer therapy based on alternating current electric field exposure, Electro-Capacitive Cancer Therapy (ECCT), has been pre-clinically tested for its effectiveness in breast tumor inhibition. However, concerns regarding the safety of electric field (EF) exposure to vital organs have been raised. In the kidney and spleen, IL-10 and TNF-α play roles in the response to inflammation. Objective: This research aimed to evaluate the safety of electric field exposure to vital organs, by assessing the expression of IL-10 and TNF-α mRNA in the kidney and spleen of rats, both with and without breast tumors. Methods: Female rats were divided into four groups; non-induction-non-therapy (NINT), non-induction-therapy (NIT), induction-non-therapy (INT), and induction-therapy (IT). Rats were induced by 7,12-dimethylbenz[a]anthracene (DMBA) at a dosage 20 mg/Kg body weight and exposed to electric fields for 10 hours, followed by a 2-hour rest period. Total RNA from the kidney and spleen was isolated, cDNA was synthesized by reverse transcription PCR, and analysis of IL-10 and TNF-α mRNA expression was performed by RT-qPCR method. Data were analyzed using the Livak formula, GraphPad Prism 9.0 software, and significance test ANOVA (α = 0.05). Results: Alternating current electric field exposure had no effects on the expression of TNF-α and IL-10 mRNA in the kidney and spleen of rats. Specifically, no effect was observed on TNF-α mRNA expression in all groups, and there was no effect on IL-10 mRNA expression in the spleen. Only the INT group showed a significant increase in IL-10 mRNA expression in the kidney (p < 0.05). Conclusion: Exposure of an alternating current electric field did not affect the relative mRNA expression of TNF-α and IL-10 in the kidneys and spleen of rats.
The effect of lemon extract (Citrus limon) on the blood sugar levels and pancreatic beta cell regeneration in alloxan-induced hyperglycemic mice Petrus Mantur; Efrisca Meliyuita Br. Damanik; Elisabeth Levina Sari Setianingrum; Prisca Pakan
Acta Biochimica Indonesiana Vol. 6 No. 1 (2023): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabiona.97

Abstract

Background: High blood sugar levels that exceed normal limits or commonly refered to as hyperglycemia, is an early symptom of diabetes mellitus. Objectives: This study explored the effect of lemon (Citrus limon) extract on blood sugar levels and pancreatic b cell regeneration in alloxan-induced hyperglycemic mice (Mus musculus). Method: This research is an experimental study using a post-test group. The sampling was carried out using a randomization method. By administering 125 mg/kg BW of alloxan, the sample was conditioned for hyperglycemia. The samples were divided into five groups: normal control, alloxan-induced control, and three treatment groups that received lemon extract with dosages of 100 mg/kg BW, 300 mg/kg BW and 500 mg/kg BW, respectively. Semiquantitative analysis was used to evaluate pancreatic damage. Results: The results showed that lemon extract can decrease blood sugar levels. Histopathological imaging revealed a significant improvement in b cell distribution and decreased vacuolization in the Langerhans islets of mice administered lemon extract. No significant differences were observed among different dosages of lemon extract (p>0.05). Conclusion: Our findings underscore the potential therapeutic benefits of lemon extract in managing blood sugar levels and promoting pancreatic b cell regeneration in alloxan-induced mice.
The correlation between plasma malondialdehyde levels and pain in adolescent females diagnosed with primary dysmenorrhea Ocktariyana Ocktariyana; Dian Lestari; Abdul Kadir; Listrianah Listrianah; Handayani Handayani
Acta Biochimica Indonesiana Vol. 6 No. 1 (2023): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.119

Abstract

Background: Endometrial inflammation factors play a crucial role in the common pathophysiology of primary dysmenorrhea. Malondialdehyde (MDA), a byproduct of inflammatory processes, might be correlated with primary dysmenorrhea in adolescents. Objective: To assess plasma MDA levels in patients with primary dysmenorrhea. Methods: This cross-sectional study involved the collection of blood samples from 23 teenage females with primary dysmenorrhea and 23 age-matched individuals without this condition. Plasma MDA levels were quantified through spectrophotometry. The independent t-test was employed to ascertain the disparity in plasma MDA levels between the two groups, while the Pearson correlation test was utilized to analyze the relationship between pain severity and oxidative stress levels. Results: Plasma MDA levels were significantly higher in females with primary dysmenorrhea than in the control group (0.631 ± 0.105 and 0.395 ± 0.076, respectively). A significant difference in oxidative stress levels was observed between the case and control groups (p<0.001). Furthermore, a robust positive correlation was observed between plasma MDA levels and pain severity in adolescent females with dysmenorrhea (r2 = 0.564, p<0.001). Conclusion: The plasma MDA is increased in adolescent females with primary dysmenorrhea.
Zinc deficiency effect on clinical features of Coronavirus Disease 2019 patients: A scoping review Moch Ikhsan Juliansyah; Lisa Adhia Garina; Mirasari Putri
Acta Biochimica Indonesiana Vol. 6 No. 1 (2023): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.123

Abstract

COVID-19 was declared a pandemic in March 2020. Various therapeutic guidelines have been established for treating COVID-19, such as anti-inflammatory, antiviral therapies, and zinc supplementation. Zinc deficiency is thought to worsen the condition of patients with COVID-19 infection. However, the effect of zinc deficiency on COVID-19 patients has not been widely reported. In this scoping review, we aim to explore the impact of zinc deficiency on the clinical features of COVID-19 patients. A comprehensive search was conducted across the databases, including PubMed, ScienceDirect, Springer Link, ProQuest, and Google Scholar, using the keywords “Zinc” AND “(COVID-19 or SARS-CoV-2)”. A total of 2,458 articles published between 2019 and 2020 were screened following the PRISMA guidelines and subjected to critical appraisal. Three articles were included, focusing on the effect of zinc deficiency on the clinical features of COVID-19 patients. As of 2022, two articles reported worsening symptoms, one described an increased hospitalization duration, and one reported worsening treatment outcomes in the zinc deficiency group. This review concludes that zinc deficiency worsens symptoms, increases the duration of hospitalization, and leads to worse outcomes in COVID-19 patients.
Evaluation of serum amylase and lipase in diagnosed Covid-19 survivors in a North Indian Hospital, Lucknow, India Nabeela Usmani; Mukhtar Ahmad; Saba Khan; Mohammad Mustufa Khan; Haseeb Ahsan; Sadaf Parveen; Roshan Alam
Acta Biochimica Indonesiana Vol. 6 No. 1 (2023): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.141

Abstract

Background: Coronavirus disease 2019 (Covid-19) is a novel disease caused by coronavirus-2 that causes severe acute respiratory syndrome (SARS-CoV-2). Although it primarily affects the respiratory system, the virus may also target the pancreas. It was suggested that upregulation of angiotensin-converting enzyme II (ACE-II) cell receptors or inflammatory cytokines play a major role in predisposing pancreatic injury in SARS-CoV-2 patients. Our aim was to conduct a critical evaluation of in order to provide an insight into the possibility of pancreatic injury. Methods: A case control study was conducted in the Department of Biochemistry IIMS&R, Integral University, Lucknow. Two groups of subjects (70 adults consisting of 35 Covid-19 survivors and 35 controls) aged between 35 to 70 years were selected for the study with mild to moderate Covid-19 diagnosed using RT-PCR/rapid antigen test and a control group of healthy individuals without history of Covid-19. The serum amylase was determined by the direct substrate method and serum lipase by the turbidimetric UV method using a semi-autoanalyzer. Results: The study showed that the serum amylase (p=0.023) and lipase (p=0.002) levels were significantly elevated in Covid-19 patients compared to the control subjects. The findings indicated that Covid-19 patients had a substantially higher mean serum amylase and lipase levels than the control subjects. Conclusions: The Covid-19 patients are prone to pancreatic injury.

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