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JURNAL ANALIS LABORATORIUM MEDIK
Published by Universitas Sari Mutiara Indonesia
ISSN : 25361475     EISSN : 2527712X     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Jurnal Analis Laboratorium Medik provides a forum for publishing the novel technologies and knowledge related to the medical laboratory technology. Scientific articles dealing with the following topics in medical laboratory technology.
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Articles 9 Documents
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Search results for , issue "Vol 7 No 2 (2022): JURNAL ANALIS LABORATORIUM MEDIK" : 9 Documents clear
Uji Presisi Kadar FE Pada Asi Metode Spektrofotometri Serapan Atom Nazula Rahma Shafriani
Jurnal Analis Laboratorium Medik Vol 7 No 2 (2022): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v7i2.3263

Abstract

One of the nutrients that babies need is iron (Fe). Iron content can be measured by atomic absorption spectrophotometry (AAS). This method is often used because it is simpler, more accurate and has high precision. This study was to determine iron levels and to determine precision of examination of iron levels in breast milk using AAS. The method of this research is the preparation of samples, tools, and materials, the manufacture of a standard solution of iron (Fe), and the calculation of the precision value of the examination of iron levels in breast milk. According to the findings, the three test groups' average Fe level was 1.1486 mg/L. This study's precision test was run based on repeatability. In this study, the precision test was conducted by measuring three groups of samples ten times. The precision test based on repeatability is declared acceptable by comparing the CV value obtained with CV-Horwitz, if the %CV obtained is less than CV-Horwitz, then the precision test based on repeatability is acceptable. Based on the measurements that have been made, CV (%) in test group A is 7.0690, test group B is 9.0686, test group C is 7.9807. These results indicate that the three test groups have CV % which is smaller than CV-Horwitz. Depending on the findings of the study, the validation of the technique for calculating Fe levels using AAS satisfies the standards for the approved value.
Uji Diagnosis NS1, IgG dan IgM Dengue Metode Immunokromatografi dan Elisa Suryanata Kesuma
Jurnal Analis Laboratorium Medik Vol 7 No 2 (2022): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v7i2.3374

Abstract

Dengue fever is a disease caused by the dengue virus (DENV). Detection of NS1 antigen can be useful for early confirmation of DENV infection. NS1 antigen can be detected from the first day of illness and lasts up to 9-10 days. Other supporting examinations are dengue virus IgG and IgM examinations. The purpose of this study was to review the sensitivity and specificity of the dengue virus NS1, IgG, and IgM tests through literature testing. This type of research is descriptive. The literature used is scientific journals which are the top journals searched using the google search engine and contains complete information and data related to the sensitivity and specificity of NS1, IgG, and IgM Dengue Virus examinations. Based on the review, it was found that the sensitivity of the NS1 antigen in the Rapid Immunochromatographic method was in the range of 48%-92% and specificity was 73%-100%, in the ELISA method the sensitivity range was 42%-84% and specificity was 89%-99%. In the examination of IgG and IgM antibodies using the Rapid Immunochromatography method, the sensitivity ranges from 6%-73% and specificity 36%-100%. Examination of IgG and IgM antibodies using the ELISA method obtained sensitivity in the range of 6%-58% and specificity 56%-100%. The results of the review conducted showed that the sensitivity of the NS1, IgG, and IgM tests for Dengue Virus with the Rapid Immunochromatography method was better than the ELISA method, but the specificity for the NS1, IgG, and IgM tests for Dengue Virus ELISA method was better than the Rapid Immunochromatography method. In addition, the Rapid Immunochromatography method of NS1 antigen examination showed higher accuracy than IgG and IgM antibodies in screening for dengue virus infection.
Identification of Staphylococcus Aureus in Patients Diabetic Ulcus In Bunda Thamrin Hospital Medan Eka Margaret Sinaga; Mahyudi
Jurnal Analis Laboratorium Medik Vol 7 No 2 (2022): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v7i2.3376

Abstract

Staphylococcus aureus can cause infection that usually presents with characteristic signs of inflammation, necrosis, and abscess formation. Staphylococcus aureus is responsible for 80% of suppurative diseases with the skin surface as its natural habitat. Skin infections and open wounds such as ulcers, burns, and surgical wounds increase the likelihood of bacterial infection and result in systemic infection. Identification of Staphylococcus aureus in diabetic ulcer patients was carried out at the Microbiology Laboratory of Bunda Thamrin Hospital Medan from February to March 2022 with a total sample of 6 people, this study is descriptive Experiment using isolation and identification methods. The results showed that of the 6 samples, 50% of the samples were contaminated with Staphylococcus aureus bacteria, 50% of the samples were contaminated with gram-negative rods. From the results of the study it can be concluded that Staphylococcus aureus is one of the bacteria that causes infection in diabetic ulcer patients. The results showed that of the 6 samples, 50% of the samples were contaminated with Staphylococcus aureus bacteria, 50% of the samples were contaminated with gram-negative rods. The results showed that of the 6 samples, 50% of the samples were contaminated with Staphylococcus aureus bacteria, 50% of the samples were contaminated with gram-negative rods. From the results of the study it can be concluded that Staphylococcus aureus is one of the bacteria that causes infection in diabetic ulcer patients.
Kualitas Bakteriologi Escherichia Coli dan Coliform pada Air di Distrik Demta Kabupaten Jayapura Tahun 2022 Fajar Bakti Kurniawan; Meidy Johana Imbiri; Asrori; Yulianus Wisma Krisna Alfreda; Asrianto; Indra Taufik Sahli; Risda Hartati
Jurnal Analis Laboratorium Medik Vol 7 No 2 (2022): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v7i2.3384

Abstract

Clean water is water used for daily needs with quality that meets hygiene requirements. Poor environmental sanitation can increase the deposit of microorganisms in water. Water containing microorganisms can cause disease. This study aims to determine the number of Escherichia coli bacteria and groups of coliform bacteria. This research includes laboratory observational research. Water samples were obtained from springs and community shelters. Identification and numeration using the MPN (most probable number) method. The media used were Lactose Broth (LB), Brillian Green Lactose Broth (BGLB) and EC Broth with three tubes. The results of the study identified the presence of Coliform and E.coli bacteria. The numerical results on Coliform show only one sample that meets the quality standards. The results of the numeration of E.coli bacteria, all samples did not meet the quality standards of the Minister of Health Regulation No. 32 of 2017. Bacterial contamination is thought to be the result of community activities using water sources as a place for bathing and washing. The conclusion of this study is that the water used for the daily needs of the people of Demta is contaminated with coliform and E.coli.
The Effectiveness of PRP on Reducing Blood Glucose Levels in Diabetic Mice Aisyara Yuliandari; Yeli Hartuti; Dea Yuni Putri Tomahu; Hartini H
Jurnal Analis Laboratorium Medik Vol 7 No 2 (2022): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v7i2.3386

Abstract

PRP contains growth factors that have the potential to repair tissue damage, such as pancreatic damage in diabetes mellitus. Pancreas damage in diabetes mellitus is characterized by hyperglycemia. This study aimed to determine the effectiveness of PRP in reducing blood glucose levels in diabetic mice. Male Swiss Webster mice divided into 5 groups (normal group, DM group, PRP1 group, PRP2 group and PRP3 group). The DM, PRP1, PRP2 and PRP3 groups were given streptozotocin 45 mg/kgBW to induce diabetes mellitus. Mice in the PRP1, PRP2, and PRP3 groups that have indicated diabetes mellitus with glucose levels > 200 mg/dL will be given PRP with a dose of 0.1 mL; 0.3 mL; 0.5 mL intraperitoneally for 4 weeks. Data on blood glucose levels were analyzed using one-way ANOVA test and LSD test. The use of PRP in various doses, namely 0.1 mL, 0.3 mL, 0.5 mL, can reduce blood glucose levels in the PRP1 group (195.8 ± 14.04 mg/dl), PRP2 group(176.6 ± 9.37 mg/dl), PRP3 group (121 ± 5.52 mg/dl) compared to the DM group (392.6 ± 18.09 mg/dl). Dose of PRP of 0.5 mL gave a better effect on pancreatic tissue repair than the PRP1 and PRP2 groups, which were characterized by glucose levels that were close to the normal group (106.8 ± 10.61 mg/dl). Based on the results of the study, PRP 0.5 mL is effective in repairing pancreatic tissue which is characterized by a decrease in blood glucose levels in diabetic mice.
Liver Tissue Examination of Mice Using 10% BNF Fixation For 6 Hours And 16 Hours Neike Octary; Indah Sari; Aristoteles
Jurnal Analis Laboratorium Medik Vol 7 No 2 (2022): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v7i2.3457

Abstract

The fixation process is the first stage in the manufacture of histopathological preparations which aims to preserve the tissue and harden the tissue, so that the tissue to be observed does not change in shape or size. BNF fixation solution (Neutral Buffer Formalin) 10% has been used as a routine fixative and It has been be the gold standard in histology laboratories for decades. The material used in this study was a 10% BNF solution because it is easier to use and it can be used to preserve tissue for a long period of time. This study aims to determine the differences in the microscopic results of the liver tissue of mice (Mus musculus) fixed with 10% BNF for 6 hours and 16 hours. This type of research is descriptive analytic. The research was carried out at the Barokah Laboratory/ dr.Mezfi Unita on 04-05 March 2022 with a total sample of 20 samples of mice liver tissue (Mus musculus). The results of the study on 10% BNF fixation for 6 hours and 16 hours showed that the average results were not good. The conclusion from the results of the study that there was no difference in the microscopic results of liver tissue that were fixed with 10% BNF for 6 hours and 16 hours using Hematorylin-Eosin staining. 10% BNF fixation with a time of 6 hours and 16 hours can be used as an alternative in histopathological examination in the short term and long term.
Gambaran Kadar Hemoglobin Pada Ibu Hamil Trimester III di Puskesmas Bandar Khalipah Dicky Yuswardi Wiratma; Budi Kurniawan; Gunawan
Jurnal Analis Laboratorium Medik Vol 7 No 2 (2022): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v7i2.3460

Abstract

Anemia is a problem that is generally experienced by pregnant women, changes in the composition of the content also affect the body's metabolism. Pregnant women tend to get anemia due to increased circulation changes to the placenta and the fetus also accumulates iron reserves. In addition, the relationship between iron and anemia is also continuous with morbidity in pregnant women. Bleeding due to anemia is the main factor in maternal mortality, which is 31.25%. The type of research used in this study is descriptive analytic. This research was conducted at Bandar Khalipah Public Health Center from May to July 2022. This examination was carried out on 10 pregnant women using the Sahli method. Based on the results of the examination, it was found that 7 people (70%) Haemoglobin increased and 3 people (30%) others did not increase. Based on the results of the examination on pregnant women who have been given Fe tablets, it can be concluded that the increase in hemoglobin levels is due to consuming iron (Fe) tablets containing iron and folic acid. Meanwhile, the hemoglobin level did not increase even though they had consumed Fe tablets because pregnant women had not received a balanced intake and diet. Pregnant women can adjust their diet, take prenatal vitamins which usually contain iron and folic acid which are good for the blood and take Fe tablets so that hemoglobin remains stable during pregnancy.
Identification Of Trichuris Trichiura Worms Eggs in 6-8 Years Old Elementary School Children At GKPS Private Elementary School, Saribudolok, Simalikuta, Simalungun Tiara rajagukguk; Erlan Aritonang
Jurnal Analis Laboratorium Medik Vol 7 No 2 (2022): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v7i2.3462

Abstract

Tricuriasis is an infection caused by the eggs of the Trichuris trichiura worm which is often found in humans, can show symptoms such as diarrhea, anemia, lack of protein energy, weight loss and accompanied by headaches and fever. Trichuris trichiura is an intestinal nematode worm habitat in the human digestive tract, including a group of worms that are transmitted through soil (Soil Transmitted Helmints). Research has been carried out at the Chemistry-Biology Laboratory, Sari Mutiara University. This study aims to identify Trichuris trichiura worm eggs in the feces of elementary school children aged 6-8 years at SD Swata GKPS Saribudolok City, Silimakuta District, Simalungun Regency in 2022.
PEMERIKSAAN Staphylococcus aureus PADA SUSU SAPI PERAH SEBELUM DAN SESUDAH DIOLAH DI PETERNAKAN ASAM KUMBANG MEDAN Apriska Dewi Sipayung; Dyna Grace Romatua Aruan; Ester Saripati Harianja
Jurnal Analis Laboratorium Medik Vol 7 No 2 (2022): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v7i2.3666

Abstract

Susu sapi perah adalah cairan yang berasal dari kambing sapi sehat, diperoleh dengan cara pemerahan yang benar, kandungan alami tidak dikurangi atau ditambah sesuatu apapun serta belum mendapatkan perlakuan apapun kecuali proses pendingan. Salah satu bakteri yang mengkontaminasi pada susu sapi adalah Staphylococcus aureus. Infeksi Staphylococcus aureus merupakan penyebab mastitis yang menimbulkan masalah kesehatan masyarakat karena susu yang berasal dari kelenjar yang terinfeksi memiliki gen enterotoksin dan dapat menyebabkan keracunan. Staphylococcus aureus mampu berkembang biak dan menyebar luas dalam tubuh dengan menghasilkan enzim koagulase sehingga bakteri ini berbahaya saat menginfeksi manusia. Tujuan penelitian ini adalah untuk mengetahui ada tidaknya bakteri Staphylococcus aureus pada susu sapi yang belum dan sudah diolah di Peternakan Asam Kumbang Medan. Jenis penelitian ini bersifat deskriptif dengan sampel sebanyak 8 yang diperiksa sebelum dan sesudah diolah. Hasil yang diperoleh dari penelitian ini di dapat 2 positif sampel susu sebelum diolah terkontaminasi Staphylococcus aureus. Sedangkan sampel yang sudah diolah tidak terdapat kontaminasi Staphylococcus aureus Milk dairy cow is a liquid derived from healthy cows, obtained by proper milking, natural content is not reduced or added anything and has not received any treatment except the cooling process. One of the bacteria that contaminates cow’s milk is Staphylococcus aureus. Staphylococcus aureus infection is a cause of mastitis that causes public health problems as it comes from infected glands possessing enterotoxin genes and can cause. Staphylococcus aureus is able to multiply and spread in the body by producing coagulase enzymes so these bacteria are harmful when infecting humans. This study aimed to determine the presence or absence of cow’s milk that has not been and has been processed in Asam Kumbang Medan. This type of research is descriptive with 8 samples that are examined before and after processing. Results obtained from this study in 2 positive samples of milk before treatment were contaminated with Staphylococcus aureus. While the sample has been processed there is no contamination from Staphylococcus aureus.

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