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INDONESIA
JURNAL ANALIS LABORATORIUM MEDIK
Published by Universitas Sari Mutiara Indonesia
ISSN : 25361475     EISSN : 2527712X     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Jurnal Analis Laboratorium Medik provides a forum for publishing the novel technologies and knowledge related to the medical laboratory technology. Scientific articles dealing with the following topics in medical laboratory technology.
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Articles 11 Documents
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Search results for , issue "Vol 8 No 1 (2023): JURNAL ANALIS LABORATORIUM MEDIK" : 11 Documents clear
FAKTOR-FAKTOR YANG MEMPENGARUHI PATOGENITAS TRICHOMONAS VAGINALIS Sresta Azahra; Lisawati Susanto
Jurnal Analis Laboratorium Medik Vol 8 No 1 (2023): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v8i1.3799

Abstract

Trichomonas vaginalis is a flagellated protozoan parasite that causes trichomoniasis. Trichomoniasis is a non-viral sexually transmitted infection and is found in the human urogenital tract. The interaction between T. vaginalis with the host is very complex and many factors influence the pathogenicity of T. vaginalis. To determine the factors that influence the pathogenicity of Trichomonas vaginalis. Literature review using the Google Scholar database. The search results for articles matched the criteria with the keyword "pathogenicity of Trichomonas vaginalis". Pathogenicity factors include changes in the trophozoite Trichomonas vaginalis to an ameboid form, cellular pathogenicity (attachment of Trichomonas vaginalis to host cells, cytotoxic and hemolytic activity, phagocytosis), interactions with vaginal flora, symbiont contribution with Trichomonas vaginalis, recognition by the host immune system, and evasion host immune system. Pathogenicity factors include changes in the trophozoite Trichomonas vaginalis to an ameboid form, cellular pathogenicity (attachment of Trichomonas vaginalis to host cells, cytotoxic and hemolytic activity, phagocytosis), interactions with vaginal flora, symbiont contribution with Trichomonas vaginalis, recognition by the host immune system, and evasion host immune system.
GAMBARAN JAMUR Trichophyton sp PADA KAKI PETUGAS DINAS LINGKUNGAN HIDUP SAMARINDA SEBERANG Putri Yani Romansyah; Supri Hartini; Sresta Azzahra
Jurnal Analis Laboratorium Medik Vol 8 No 1 (2023): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v8i1.3801

Abstract

Prevalensi penyakit dermatofitosis di Asia mencapai 35,6%. Berdasarkan hasil penelitian menyatakan bahwa 80-90% kasus tinea pedis dan tinea unguium disebabkan oleh jamur dermatofita, terutama Trichophyton rubrum dan Trichophyton mentagrophytes. Petugas kebersihan adalah contoh pekerjaan yang kesehariannya menggunakan sepatu yang tertutup dengan waktu cukup lama, lapangan kerja yang kotor, panas dan lembab menjadi faktor yang memudahkan terjadinya infeksi jamur pada kaki dan kuku kaki. Penelitian ini bertujuan untuk mengetahui gambaran jamur Trichophyton sp pada kerokan kulit dan kuku kaki petugas kebersihan DLH. Jenis penelitian bersifat deskriptif dengan melakukan observasi kuesioner terhadap petugas kebersihan DLH di Samarinda Seberang dan pemeriksaan secara makroskopis dan mikroskopis. Responden pada penelitian ini berjumlah 17 orang petugas kebersihan berjenis kelamin laki-laki yang akan diambil kerokan kulit dan kuku kaki, kemudian dilakukan pemeriksaan makroskopis dan mikroskopis. Hasil penelitian, didapatkan responden yang terinfeksi jamur Trichophyton sp sebanyak 6 orang (35,3%) dan yang tidak terinfeksi sebanyak 11 orang (64,7%). Petugas kebersihan DLH di Samarinda Seberang lebih banyak berusia 30-35 tahun (47%) dengan lama bekerja 1-3 dan 4-6 tahun (35%). Responden yang rutin menggunakan APD sebanyak 11 orang (64,7%) dan rutin mencuci kaki sebanyak 15 orang (88,2%). Penggunaan APD dan kebiasaan mencuci tangan dan kaki menjadi salah satu faktor terinfeksi jamur Trichophyton sp. Disimpulkan bahwa terdapat petugas kebersihan DLH yang terinfeksi jamur Trichophyton sp baik yang menggunakan sepatu boot dan mencuci kaki/tangan.
DESAIN PRIMER SECARA IN SILICO UNTUK AMPLIFIKASI GEN tcdA BAKTERI CLOSTRIDIUM DEFFILCILE I Gusti Agung Ayu Satwikha Dewi; Amellya Octifani
Jurnal Analis Laboratorium Medik Vol 8 No 1 (2023): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v8i1.3806

Abstract

Antibiotics are drugs used to fight infections caused by bacteria. C. difficile bacteria is a bacterium that causes Clostridium difficile infection (ICD). Toxin A of C. difficile bacteria is exotoxin A (tcdA) which is a virulence factor that causes disease. Toxin A can damage the intestinal mucosa and cause bleeding. The cost of examining and treating patients with AAD and PMC is very high, so the dosage must be determined correctly. Laboratory tests function to determine the presence or absence of an ICD. The discovery of specific biomarkers is a discovery priority to improve the quality of laboratory tests. Specific primary design is an important step in all types of laboratory examination methods, one of which is molecular examination by PCR. The primary design must have specific properties so as to be efficient at target amplification. This study aims to obtain a primer design that can be used to limit the amplification area of the TcdA gene in silico. This study used a literature study from the NCBI genbank to obtain the tcdA gene sequence of C. defficile bacteria with accession number DQ902560.1. The tcdA gene sequence of C. difficile bacteria was analyzed using Primer3Plus to determine whether there is hairpin and dimer formation. Furthermore, identification was carried out using web-based in silico PCR to determine the number of amplicon and visualization on the electrophoretic gel. The level of tcdA gene homology in the species group in the BLAST test. The results showed that the best primer design for in silico detection of the tcdA gene of C. difficile was the forward primer 5'-GCAGATGCCAAGAGACACAC-3' and the reverse primer 3'-GCAGATGCCAAGACACACAC-5'. The primary design is able to amplify the tcdA gene region with a size of 247 amplicons.
PERBEDAAN NILAI INDEKS MENTZER, HBA2 DAN STATUS BESI PADA ANEMIA DEFSIENSI BESI DAN THALASEMIA PADA PASIEN ANAK DI RSUD ULIN BANJARMASIN Marini Aprida Yanti; Wahdah Norsiah; Neni Oktiyani; Ahmad Muhlisin
Jurnal Analis Laboratorium Medik Vol 8 No 1 (2023): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v8i1.3818

Abstract

Anemia defisiensi besi dan thalassemia memiliki gambaran morfologi darah hipokrom yang sama, oleh karena itu perlu dibedakan dengan dilakukannya sebuah pemeriksaan. Tujuan menggunakan menzter indeks untuk membedakan dan mendeteksi subyek yang memerlukan tindak lanjut yang tepat dan untuk mengurangi biaya yang tidak perlu. Diagnosis banding antara anemia defisiensi besi dan thalassemia didasarkan pada hasil HbA2 elektroforesis, kadar status besi berupa kadar feritin serum, Serum Iron (SI), Total Iron Binding Capacity (TIBC) dan Saturasi Transferin (ST). Tujuan penelitian ini adalah untuk mengetahui perbedaan nilai indeks Mentzer, HbA2 dan Status besi antara anemia defisiensi besi dan thalassemia. Manfaat penelitian ini memberikan informasi jika pemeriksaan indeks mentzer dapat digunakan sebagai skrining awal untuk membedakan Anemia Defisiensi Besi dan Thalasemi. Jenis penelitian ini survei analitik dengan rancangan studi komparatif. Sampel diambil secara Total sampling dengan jumlah 20 pasien anak Anemia defisiensi Besi dan 20 pasien anak Thalasemia di RSUD Ulin Banjarmasin pada bulan Juli-Agustus 2022. Hasil analisis statistik menggunakan uji Independent Samples Test menunjukkan nilai signifikansi untuk menzter indeks 0,000, Total iron binding capacity 0,002 dan HbA2 0,000. Dengan menggunakan analisis uji Mann-Whitney menunjukkan nilai signifikansi pada serum iron sebesar 0,000, saturasi transferin 0,000 dan ferritin 0,000. Maka H1 diterima karena nilai sig < 0,05. Kesimpulannya penelitian ini terdapat perbedaan bermakna antara nilai indeks mentzer, HbA2, feritin, serum iron, total iron binding capacity, saturasi transferin antara pasien anemia defisiensi besi dan thalassemia pada pasien anak di RSUD Ulin Banjarmasin
ANALISIS LOGAM TIMBAL (Pb) LIPSTIK YANG BEREDAR DI PASAR GAMPING SLEMAN YOGYAKARTA Bagus Rozaq Sembada; Isnin Aulia Ulfah Mu’awanah; Arif Yusuf Wicaksana
Jurnal Analis Laboratorium Medik Vol 8 No 1 (2023): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v8i1.3832

Abstract

Pada zaman modern seperti sekarang ini sangat banyak orang yang menggunakan produk kosmetik terkhusus di Indonesia, hal ini menyebabkan penggunaan kosmetik di Indonesia semakin meningkat dengan sangat pesat. Beberapa produk kosmetik yang sering digunakan oleh masyarakat adalah eye-liner, concealer, pensil alis bedak, lisptik dan lainnya. Lipstik merupakan produk kosmetik yang digunakan untuk mewarnai bibir dengan sentuhan artistik sehingga dapat meningkatkan estetika dalam riasan wajah. Beberapa produk lipstik yang beredar di pasaran mengandung timbal, bahan dasar lipstik ini merupakan senyawa kimia yang berbahaya bagi manusia apabila digunakan dalam jangka waktu panjang. Sehingga untuk mengetahui adanya kadar timbal dalam lipstik, peneliti menganalisis menggunakan spektrofotometer serapan atom. Penelitian ini bertujuan untuk mengetahui kadar timbal pada lipstik yang beredar di pasar Gamping Yogyakarta. Hasil analisa kadar logam berat Timbal dari 13 sampel lipstik pada penelitian ini didapatkan sampel lipstik dengan kadar logam timbal tertinggi adalah 10,896 µg/g. Sedangkan kadar timbal terendah pada penelitian ini adalah 0,471 µg/g. Sampel yang di uji mengandung timbal, kadar timbal pada lipstik berkisar antara 0,096- 10,896 µg/g dan semua sampel memenuhi syarat BPOM.
ANALISA BAKTERI TAHAN ASAM PADA DAHAK PEROKOK AKTIF PENDERITA TUBERKULOSIS PARU Eka Margaretha Sinaga; Maniur A Siahaan; Apriska Dewi Sipayung
Jurnal Analis Laboratorium Medik Vol 8 No 1 (2023): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v8i1.3834

Abstract

Acid fast bacteria (AFB) are cell walls containing complex lipids so that in Ziehl-Neelsen staining they can bind dyes because they are not washed off by 3% HCl Alcohol (Alcoholic Acid). One of the host factors in pulmonary tuberculosis is smoking habits and exposure. Smoking habits will damage the defense mechanism of the lungs, which causes the lungs to be more easily infected with tuberculosis germs. The purpose of this study was to determine the results of analysis of acid-fast bacteria in the sputum of active smokers. The research method used was descriptive cross-sectional by examining acid-fast bacteria in the sputum of active smokers suspected of having pulmonary tuberculosis by microscopic examination of sputum (direct smear smear) using the Ziehl-Neelsen staining method. smear examination with Ziehl-Neelsen staining in patients with tuberculosis at Putri Hijau Hospital in 2022, totaling 20 samples, found 4 positive results and 16 samples with negative results.
IDENTIFIKASI SOIL TRANSMITTED HELMINT PADA FESES TERNAK BABI MENGGUNAKAN METODE DIRECT SLIDE Monika Putri Solikah; Havida Widyastuti
Jurnal Analis Laboratorium Medik Vol 8 No 1 (2023): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v8i1.3880

Abstract

Until now, parasitic diseases that infect humans and animals have not been considered in Indonesia because these diseases do not cause clinical symptoms (subclinical). Gastrointestinal disease caused by worm parasites is a systemic disease that attacks the digestive tract of cattle. Traditional pig farming methods and poor hygiene are the main reasons for the spread of zoonoses that can be transmitted from pigs to humans. This study aims to identify nematode hosts by examining worm eggs in pig feces using the direct slide method. From a total of 20 stool samples, 3 samples were positive for nematode worm eggs. A stool sample that is identified as positive for worm eggs determines the level of parasitic infestation. Worm egg detection was carried out in the laboratory of the Faculty of Health Sciences University and analyzed descriptively, then the number of worm eggs was counted to determine the prevalence of endoparasites.
PENGARUH PENGGUNAAN MINYAK KELAPA MURNI SEBAGAI LARUTAN CLEARING PADA SEDIAAN HEPAR MENCIT Eko Naning Sofyanita; Nabila Azahra
Jurnal Analis Laboratorium Medik Vol 8 No 1 (2023): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v8i1.3945

Abstract

Salah satu tahapan dalam pemrosesan jaringan dengan histoteknik adalah clearing yang menggunakan larutan xylol. Xylol sering digunakan sebagai agen clearing karena prosesnya cepat dan harganya tidak terlalu mahal, tetapi penggunaan xylol sudah mulai ditinggalkan karena sifatnya yang karsinogenik. Minyak mempunyai karakter bersifat non polar sehingga dengan sifat non polar dari minyak kelapa ini dapat menghilangkan sisa parafin yang terdapat pada jaringan dan dapat digunakan sebagai alternatif larutan clearing. Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaan minyak kelapa murni (virgin coconut oil) sebagai alternatif larutan clearing terhadap gambaran makroskopis jaringan hepar mencit (Mus musculus). Metode penelitian ini menggunakan penelitian eksperimental dengan pendekatan one-shot case study. Hasil penelitian ini dilihat dari parameter transparansi dan tekstur menghasilkan 16 (100%), preparat baik sedangkan parameter kemudahan pemotongan didapat 10 sediaan baik (62,5%). Uji Mann-Whitney didapatkan nilai p-value < 0,05 maka ada perbedaan kualitas sediaan jaringan hepar mencit (Mus musculus) yang dijernihkan menggunakan xylol dan minyak kelapa murni (virgin coconut oil). Kesimpulan pada penelitian ini yaitu minyak kelapa murni (virgin coconut oil) dapat digunakan sebagai alternatif larutan clearing berdasarkan gambaran makroskopis jaringan hepar mencit (Mus musculus) dengan hasil kualitas sediaan jaringan yang baik tetapi kualitasnya masih tidak semaksimal penggunaan larutan xylol.
PENGARUH MENCUCI TANGAN TERHADAP ANGKA KUMAN PADA TELAPAK TANGAN SISWA SEKOLAH DASAR Rida Maulina; Tiara Dini Harlita; Nursalinda Kusumawati
Jurnal Analis Laboratorium Medik Vol 8 No 1 (2023): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v8i1.3959

Abstract

Washing hands with soap is one method of protecting the body from many infectious illnesses. Following the guidelines of the World Health Organization, effective hand washing involves six stages. The purpose of this study is to ascertain how proper and good hand washing habits affect the quantity of germs on the palms of pupils at State Elementary School 005 Samarinda. Purposive sampling was utilized to choose 31 samples for a quasi-experimental study with a one group pretest-posttest design. Through counseling, samples taken in the form of palm swabs and examined before and after hand washing were evaluated using the Total Plate Number (ALT) technique with dilutions ranging from 101 to 102. The Wilcoxon Test technique was used to assess the test findings using univariate and bivariate analysis. According to studies, there are typically 78 CFU/cm2 of germs on the hands before washing them, and there are 10 CFU/cm2 of germs after washing them with soap, with a 79% reduction in the quantity of germs on the palms. The Wilcoxon test findings showed a p-value of 0.000, indicating that there was a reduction in the number of germs on primary school children' palms both before and after washing their hands. Always wash your hands before and after carrying out activities.
ANGKA KUMAN PADA GAGANG PINTU FASILITAS PELAYANAN KESEHATAN DI WILAYAH SAMARINDA SEBERANG Desy Natha Auliah Putri Palandrai; Lamri; Dita Irianti Rukmana
Jurnal Analis Laboratorium Medik Vol 8 No 1 (2023): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v8i1.3961

Abstract

Health service facilities are places where health services are carried out promotively, preventively and become one of the places where diseases and infections spread. The spread of disease infection through germs on door handles which are objects that are very often used every day and have the potential to be a source of spread of germs from one person to another. This study aims to determine the number of germs on the doorknobs of health care facilities in the opposite Samarinda area. This type of research is descriptive which describes the results of the total germ number on the door handle, with the number of door handle swab samples in this study 27 samples, each of which is divided into 9 samples by testing using the Total Plate Number method with Plate Count Agar media then incubated 1 x 24 hours. The results of this study can be concluded that 63% meet quality standards with a value of less than 10 CFU / cm2 and 37% do not meet standards that exceed the number of quality standards more than 10 CFU / cm2.

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