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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,053 Documents
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Kadar dan Daya Luteolitik PGF2? Produksi Sel Monolayer Vesikula Seminalis dan Endometrium Sapi Bali (PROSTAGLANDIN F2? CONCENTRATIONS OF BALI CATTLE ENDOMETRIAL AND SEMINAL VESICLE MONOLAYER CELLS CULTURE PRODUCTS AND ITS IN VITRO TEST ON LUTEAL MONOLAYER Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana; Laba Mahaputra
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aims of this research were to determine PGF2? concentration the produced by bali cattlesendometrial and seminal vesicle monolayer cell culture and in vitro luteolytic ability on luteal monolayercell culture. The endometrial and seminal vesicle epithelial cell of bali cattle were cultured in tissueculture medium (TCM) 199 growth medium supplemented with 10% fetal calf serum and 10% EstrusMare Serum. The cells were cultured at 1.9 x 106 density per ml medium. Then Followed by incubation at38.50 C in 5% CO2 atmosphere for 12 days. The level of PGF2? in the cell culture medium were assayed byRadioimmnuassay (RIA) technique. The luteal cells were cultured in 9 days incubation and divided into 2groups. Group I were added with 10% of cell culture product and group II were added with 1,25 mgdinoprost/ml. The level of progesterone produced by luteal cell culture was measured at day 9th and 11thincubation. The result showed concentration of PGF2? cell product of seminal vesicle cell culture wassignificantly higher (P < 0.05) compared to endometrial cell culture. There was no significant difference(P>0.05) in luteolytic ability between PGF2? cell culture product and dinoprost. In conclusion, the PGF2?could be produced by monolayer cell culture of bali cattle is endometrial and seminal vesicle epithelialcells more over they have similar ability with dinoprost in luteolytic ability.
Respons Imunoglobulin-G dan Imunoglobulin-M Mencit yang Diberi Ekstrak Methanol Alga Biru Hijau dan Diinfeksi Dengan Takizoit Sorta Basar Ida Simanjuntak; Sukarti Moeljopawiro; Wayan Tunas Artama; Subagus Wahyuono
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Toxoplasmosis is an infectious disease caused by Toxoplasma gondii. This disease could severelyaffect humans and animals. Up to now there has been no simple treatment to fight toxoplasmosis. Aprospective alternative treatment to overcome this problem is by increasing immunity of the body using animmunostimulant such as Spirulina platensis. The aims of this research were to observe the potency of S.platensis as an immunostimulant and to find the most potential fraction of S. Platensis that can increasethe responses of IgG and IgM antibodies againts toxoplasma. The responses of these antibodies weremeasured using ELISA method. The isolation of compounds from S. platensis using Preparative ThinLayer Chromatography (PTLC) found three fractions which were a top fraction (I), a middle fraction (II),and a lower fraction (III). Forty-eight mice used in this research were divided into four different groupswith 12 mice in each group and treated differently. The top, middle, and lower fractions of S. platensis wereadministered orally to three groups of mice respectively at dose of 3mg/ml for each mouse while the micein the fourth group were kept as untreated controls. The treatment was conducted for 14 days consecutivelyand on the next day, all mice, including the controls, were challenged with tachizoit. The effect of S.platensisfractions on the responses of IgG and IgM antibodies were then measured at various time intervals, i.e. day0 (before infection) and day 1, 2, and 3 after infection. The results showed that IgG response increased inthe day 0 (2.504 OD) and the day 3 after infection (2.608 OD) while IgM response increased in day 1 afterinfection (2.898 OD). In conclusion, S. platensis was an immunostimulant and the middle fraction (II) of S.Platensis was the most potential fraction to increase immunity againts toxoplasma .
Produksi dan Isolasi Protein Membran Stadium Bradizoit Toxoplasma gondii : Suatu Usaha untuk Mendapatkan Material Diagnostik dalam Mendiagnosa Toksoplasmo Muhammad Hanafiah; Wisnu Nurcahyo; Mufti Kamaruddin; Fadrial Karmil
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was conducted to isolate the membrane protein of Toxoplasma gondii at bradyzoid stage forthe development of intradermal diagnostic test in livestock infected by the parasite. Toxoplasma wasinitially collected from meat of goat serologically positive to the parasite. The infected meat was then fedinto uninfected cat to obtain oocyst. The oocyst was inoculated into the stomach of mice to produce tachyzoitwhich in turn produce cyst in tissue known as bradyzoit . The membrane protein was then isolated from thebradyzoit. The protein was analysed by sodium dodecyl sulfate electrophoresis (SDS-PAGE). The dataobtained were presented descriptively. The protein concentration isolated from each mouse infected at thedose of 1x107 oocysts was 11.91 mg. Two protein bands specific for bradyzoit were identified at 97.72 kDaand 67.60 kDa.
Penggunaan Protein Membran Stadium Bradizoit Toxoplasma gondii untuk Mendiagnosis Toksoplasmosis dengan Metode Intradermal Test Muhammad Hanafiah; Wisnu Nurcahyo; Dwinna Aliza; Teuku Fadrial Karmil
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A research was conducted to find out an alternative diagnose in detecting toxoplasmosis in livestock/animal using intradermal test  from protein membrane of  T. gondii bradizoite stage. Local isolate ofmembrane T. gondii bradizoite stage  was used in the research. Ten of domestic sheep with the age of ± 1year and 10 mice strain Balb/c with the age ± 2 month were used in this research. The reaction ofhypersensitivity on the skin post protein membrane bradyzoite injection was indicated by the process ofskin thickening. The diameter skin thickening was measured using cutimeter, in which diameter e” 10 mmindicated positive diagnose. The result showed that optimal dosage of membran protein bradyzoite thatcould be applied to detect toxoplasmosis in livestock and animal using intradermal test were 0,6 ml and0,2 ml for sheep and mice respectively. The sensitivity and specificity level of antigen use (protein membrane)of T. gondii bradizoite stage from local isolate to diagnose toxoplasmosis in mice using intradermal testwere: 85.0 %  and 66.6 % respectively, while in sheep the sensitivity and specificity level were 85.0 % and66.6 % respectively. It can be concluded that  intradermal  test was appropriate to be implemented fordetecting toxoplasmosis in sheep and mice induced with tachyzoite T. gondii.
Pengimbuhan Kunyit dan Seng Oksida dalam Pakan Meningkatkan Kemampuan Ayam Pedaging dalam Mengeliminasi Tantangan Infeksi Escherichia coli (SUPPLEMENTATION CUCURMIN AND ZINC OXIDE INCREASE THE ABILITY OF BROILER CHICKENS IN ELIMINATING ESCHERICHIA COLI CH Sus Derthi Widhyari; Ietje Wientarsih
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aims to determine the effect of the feed additive (zinc and herbs) in leucocyte profiles,performance, and observe the ability of broiler on E. coli challenge. A total of 160 chickens were dividedinto four groups: Group K-control negativewere given basal diet; Group two were given fed basal, turmeric1.5% + ZnO 180 ppm; Group two were given fed basal, garlic powder 2.5% + Zn0 180 ppm; and group K+ weregiven basal diet and treatment with antibiotics. At the age of three weeks all groups were challenged orallyby inoculation with E.coli at dose of 108CFU/mL. Observed parameters include performance (weight gain,consumption) and total leukocytes. Weight gain, consumption were observed at one to five weeks of age,whereas blood samplings for the examination of leukocytes were performed at week three (pre infection),week four and five (one week and two weeks after infection with E.coli). The results showed that thechicken body weight from age one to three weeks was increased sharply. One week after infection weightloss seemed to be decreased. The largest decrease was observed in the group given the combination ofgarlic-Zn, while providing a combination of curcumin-Zn shows that the weight tends to increase. Rationconsumption showed the same pattern as body weight. The highest consumption at the end of the studywas found in the group given the combination of curcumin - Zn, the lowest was in the garlic-Zn. Highleukocyte cells level at one week post infection, showing an animal in a state of infection and the leucocytedecreasing again at two weeks post infection. The conditions indicate the ability of chickens in eliminatinginfectious agents. These results explain the combination of curcumin-Zn showed the best results comparedwith other treatments. Providing supplementation of curcumin - Zn on chicken improved the ability toeliminate E.coli, leukocyte cell profile, and performance.
Deteksi Gen Resisten Insektisida Organofosfat pada Aedes aegypti di Banyuwangi, Jawa Timur Menggunakan Polymerase Chain Reaction Aditya Yudhana; Ratih Novita Praja; Maya Nurwartanti Yunita
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.158 KB) | DOI: 10.19087/jveteriner.2017.18.3.446

Abstract

Banyuwangi is still listed as areas not completely free from hemorrhagic dengue fever outbreak. Changes in the genome of Aedes aegypti mosquito as the main vector of dengue virus is estimated to be the cause of difficulty in controlling the disease. Expression of Voltage Gated Sodium Channel (VGSC) genes can be used as an important indication of Aedes aegypti mosquitoes resistancy to certain groups insecticides. The purpose of this study was to detect the Voltage Gated Sodium Channel (VGSC) genes coding in Aedes aegypti using Polymerase Chain Reaction (PCR); as it is related to the mechanism of resistance to organophosphate insecticides groups. Results of resistency by susceptibility test showed that Aedes aegypti mosquito samples from three district ie. Tegaldlimo, Purwoharjo, and Banyuwangi were resistant to malathion 0.8% insecticide (organophosphates group). PCR results detected gen band with 250 bp length, which confirmed that insecticide resistance gene has been detected in the tested samples. Based on this study results, it is recommended to use alternative insecticides apart from organophosphate group for dengue vector control in Banyuwangi. ABSTRAK Kabupaten Banyuwangi masih tercatat sebagai daerah yang belum sepenuhnya bebas dari wabah demam berdarah dengue (DBD). Perubahan genom nyamuk Aedes aegypti sebagai vektor utama virus dengue diperkirakan menjadi penyebab sulitnya pengendalian penyakit DBD. Ekspresi gen Voltage Gated Sodium Channel (VGSC) dapat dijadikan indikasi penting sejauh mana perkembangan nyamuk Aedes aegypti resisten terhadap insektisida golongan tertentu. Tujuan penelitian ini adalah untuk mendeteksi gen penyandi VGSC pada nyamuk A. aegypti yang berkaitan dengan mekanisme resistensi terhadap insektisida golongan organofosfat menggunakan teknik Polymerase Chain Reaction (PCR). Hasil uji resistensi susceptibility test menunjukkan sampel nyamuk A. aegypti dari tiga kecamatan di Kabupaten Banyuwangi yaitu Kecamatan Tegaldlimo, Purwoharjo, dan Banyuwangi resisten terhadap insektisida malathion 0,8 % (kelompok organofosfat). Hasil deteksi gen melalui PCR didapatkan band yang muncul dengan panjang 250 bp. Hal tersebut menunjukkan bahwa gen pembawa resistensi insektisida (VGSC) telah terdeteksi pada sampel yang diuji. Berdasarkan hasil penelitian, disarankan untuk pengendalian vektor DBD di Kabupaten Banyuwangi dengan menggunakan alternatif insektisida selain dari golongan organofosfat.
Faktor-Faktor Risiko Rabies pada Anjing di Bali (RISK FACTORS ANALYSIS FOR RABIES INDOGS IN BALI) I Nyoman Dibia; Bambang Sumiarto; Heru Susetya; Anak Agung Gde Putra; Helen Scott-Orr
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The efforts to eradicate rabies in Bali have been done for more than three years. However, therabiescases is still spreading. Thus, rabies virus continues to infect humans. A case-control study wasconducted to identify the risk factors associated with rabid dog in Bali. Cases were defined as dogsconfirmed having rabies by direct fluorescent antibody test (dFAT). Determination of sample amount ineach district was taken proportionally and samples were taken by using simple random sampling. A totalof 51 rabid dog cases between 2010 and 2011 and 102 uninfected rabies dogs as control were used in thisstudy. Possible associated factors were obtained by doing questionnaire. The data were subsequentlyanalyzed using chi-square (X2) and odds-ratio (OR) for possible association, which were ultimately analyzedby means of logistic regression to build up of model. This study revealed that factors associated with rabiddog were the status of rabies vaccination (X2= 55.538; P= 0.000; OR= 19.133; 95% CI= 8.015<OR<45.678),contact with other dog (X2= 43.659; P= 0.000; OR= 12.551; 95% CI= 5.541<OR<28.430),condition of dog(X2= 9.994; P= 0.002; OR= 3.019; 95% CI= 1.504<OR<6.058),number of raised dog (X2= 9.284; P= 0.002;OR= 2.962; 95% CI= 1.455<OR<6.027), and veterinary care (X2= 5.258; P= 0.022; OR= 2.444; 95% CI=1.125<OR<5.310). It was found an appropriate logit model to estimate probability of rabid dog events inBali province as follows : Logit Pr (rabies=1| x) = - 4.413 + 3.919 (status of rabies vaccination) + 3.457(contact with other dog). This study is expected to be used as a reference in order to improve rabies controleffectiveness in Bali.
Wabah Penyakit Ingusan (Malignant Catarrhal Fever) pada Sapi Bali di Pulau Lombok, Nusa Tenggara Barat, Indonesia (OUTBREAK OF MALIGNANT CATARRHAL FEVER IN BALI CATTLE IN LOMBOK ISLAND, WEST NUSA TENGGARA, INDONESIA) Agus Wiyono; Rini Damayanti
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Malignant catarrhal fever is a fatal disease in Bali cattle caused by Ovine Herpesvirus-2 (OvHV-2) carried by sheep. The virus has not been isolated and the vaccine is not available yet. Diagnosis in the field is observed by clinical and epidemiological changes confirmed by histopathology and antigen detection by nested polymerase chain reaction (PCR). The purpose of this paper is to provide clinical, pathological and epidemiological features as well as the results of nested PCR examination of MCF outbreaks in Bali cattle that occurred in Lombok island, West Nusa Tenggara. During January to September 2016, 20 out of 231 cattle were dead and 16 of them had depression, fever, corneal opacity, hypersalivation, purulent nasal discharges and superficial lymph nodes enlargement. There were 10 lambing sheep with the cage were located between 50 to 200 m with the cattle barn which was separated by a barrier wall. Gross lesions were characterized by diffuse hemorrhage in tracheal mucosa, epicard, abomasum, small intestine and urinary bladder and enlargement of superficial lymph nodes. Microscopiccally, pathognomonic lesions namely necrotizing vasculitis was found in the brain, trachea, cornea, lung, heart, liver, kidney and urinary bladder. Using nested PCR, OvHV-2 virus was detected in peripheral blood leucocytes/PBL in two cattle surviving from MCF and in ocular, nasal and vaginal swab from ten adult sheep. Clinical, pathological, epidemiological and molecular examinations indicate that 16 cattle were confirmed having MCF transmitted from infected adult sheep grazing and the viruses were spread by the lambing sheep kept closely to the cattle barn.
Aktivitas Invitro Senyawa Antimikroba Streptococcus lactis (INVITRO ACTIVITY ANTIMICROBIAL SUBSTANCE PRODUCED BY STREPTOCOCCUS LACTIS) I Nyoman Suarsana; Maria Bintang; Iwan Harjono Utama; Ni Gusti Agung Ayu Suartini
Jurnal Veteriner Vol 2 No 1 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Aktivitas Invitro Senyawa Antimikroba Streptococcus lactis (INVITRO ACTIVITY ANTIMICROBIAL SUBSTANCE PRODUCED BY STREPTOCOCCUS LACTIS)
Determination of Gestational Age and Observation of Kacang Goat Fetal Development during 60 Days of Pregnancy by Using Transcutaneous Ultrasonography Arman Sayuti; Khairiah Khairiah; Tongku Nizwan Siregar; Juli Melia; Syafruddin Syafruddin; Erdiansyah Rahmi; Herrialfian Herrialfian; Mahdi Abrar; Budianto Panjaitan; Razali Daud
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.184 KB) | DOI: 10.19087/jveteriner.2019.20.4.534

Abstract

This study was aimed to observe correlation between gestational age and fetal growth of Kacang goat for 60 days after mating with transcutaneous ultrasonography visualization. Three female goats of productive age with normal estrus cycle and one male goat were used in this study. The female goats were synchronized with double injection of prostaglandin F2á (PGF2á) intramuscularly with dosage of 1 mL for each female goat with 11 days’ time interval. Observation of oestrus was conducted using male goat after being synchronized. When the sign of estrus were prominent, the female goats were naturally mated. The result was pregnant positive for one female goat. Detection of early pregnancy was observed on the 24th days after mating, with the result of 6.77 mm length embryo by isoechogenic visualization. On 35th days of pregnancy, fetal could be seen clearly in isoechogenic to hyperechogenic visualization with head diameter and fetal length were 17.4 mm and 36.2 mm, consecutively. The observation also found the placentom. On day 45 of pregnancy, head diameter and fetal length were 21.8 mm and 40.6 mm. Later on day 49 of pregnancy, the size of gestational saccus was 44.1 mm, with head diameter of 25 mm, and average placentom diameter size of 12.4 mm. On day 53 of pregnancy, head diameter was 25.2 mm with fetal length of 63.6 mm and placentom diameter of 15 mm. On day 56 of pregnancy, vertebrae of fetal were observed using hyperechogenic visualization and placentom was measured 17.9 mm in diameter. On day 60 of pregnancy, we observed that the fetal length was 79.8 mm and the organs such as eyes, heart, liver, os costae, and fetal extremities, could be observed clearly. The size of developing fetal and organs would grow along with the addition of gestational age.

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