Sundoro, Ali
Yayasan Lingkar Studi Bedah Plastik

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Antibacterial Activity of Indonesian Local Honey Against Strains of P. Aeruginosa, S. Aureus and MRSA K. P., Ayu Diah; Sundoro, Ali; Sudjatmiko, Gentur
Jurnal Plastik Rekonstruksi Vol. 1 No. 2 (2012): March Issue
Publisher : Lingkar Studi Bedah Plastik Foundation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (773.441 KB) | DOI: 10.14228/jpr.v1i2.54

Abstract

Background: Honey has been used in wound care since ancient times. Many publications attest antibacterial activity of manuka honey against Pseudomonas aeruginosa, S. aureus, and Methicillin-resistant S. aureus (MRSA). However, antibacterial effect of local honey from Indonesia has never been studied and compared to medical grade honey before. This study aim to compare antibacterial activity of local honey and manuka honey against P. aeruginosa, S. aureus and MRSA.Methods: The honeys were tested for their antimicrobial activities with broth dilution method. Different concentrations of honey were prepared in reaction tubes. Strains of P.aeruginosa, S. aureus, and MRSA were grown in nutrient broth. These strains of bacteria then added to the different concentrations of honey. Minimum Inhibitory Concentrations (MIC) is the lowest concentration of the honey that yielded no growth of bacteria.Results: MIC of local honey for P. aeruginosa is 50%, for S. aureus is 100% and for MRSA is 100%. MIC of manuka honey for P. aeruginosa is 12,5%, for S. aureus is 25%, and for MRSA is 12,5%.Conclusion: Nusantara Manuka have the similar antibacterial activity spectrum against P. aeruginosa, MRSA and S. aureus, but it must be given in greater concentration to exert the same level of antibacterial activity with Manuka honey.
Management of Root Like Man: Case from Gatot Soebroto, The Indonesian Army National Hospital Sundoro, Ali; Supit, Laureen; Surachman, Asrofi S.; Adam, Harun; Budiman, Budiman; Guntoro, Guntoro
Jurnal Plastik Rekonstruksi Vol. 1 No. 3 (2012): May Issue
Publisher : Lingkar Studi Bedah Plastik Foundation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (616.736 KB) | DOI: 10.14228/jpr.v1i3.63

Abstract

Background: Patients and clinicians experience the frustration of cutaneous viral warts due to human papilloma virus (HPV) infection. Verruca vulgaris, the common warts, appear in various forms at different body sites. Over 118 types of papilloma virus have been identified. The most common cutaneous warts are caused by types 1, 2, 4, 27, 57, and 19 HPV. The rare cases of generalized warts around the world are suspected to be caused by type 2 HPV. There are many treatment modalities for this disease but none is 100% effective yet. Patient and Method :We report a 39-year-old male with generalized warts on various body parts (scalp, forehead, trunk, arms, lower leg and foot). The team consist of plastic surgeons, dermatologist, psychiatrist, pathologist, medical rehabilitationist, and internist. The treatment goals were to remove all warts, prevent recurrence of the disease, and improve the immunological status of the patient. Serial excisions were performed and closure attained using a combination of primary sutures, skin grafts (split- and full-thickness), and skin flaps. 5 Fluorouracil was administered on the raw surfaces before closure. Staged excisions were performed 11 times, with 3-4 weeks interval over a1-year period. Interferon and cimetidine injection were also given to augment the immune system.Results : All lesions were successfully excised and reconstructed. On a few fingers, hypertrophic scar and minor contracture ensued after closure by skin graft. Patient is able to carry out daily living activities independently. Patient was profoundly delighted and his quality of life improved.Summary : Management of a rare generalized verruca vulgaris through a team approach, by multiple staged excision, 5-FU application before defect closure, combined with antiviral therapy, proved effective in a case.
Comparison of Physical–Chemical Characteristic and Antibacterial Effect Between Manuka Honey and Local Honey Sundoro, Ali; K., Nadia; A., Nur; Sudjatmiko, Gentur; Tedjo, A.
Jurnal Plastik Rekonstruksi Vol. 1 No. 3 (2012): May Issue
Publisher : Lingkar Studi Bedah Plastik Foundation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (434.793 KB) | DOI: 10.14228/jpr.v1i3.74

Abstract

Background:Honey has positive effects on wound healing due to its properties to decrease inflammatory reaction, assist wound debridement, lessen wound odor, help moisturize the wound, promote wound healing and has antibacterial effect. In this research we try to confirm the benefit of using Indonesian local honey by comparing its efficacy with Manuka honey, which is already known yet still costly and not readily available in Indonesia.Methods: Two kinds of honey above were compared for its physical and chemical properties of antibacterial effects and safety, its acidity (pH) using pH meter, osmotic pressure with indirect measurement using hygroscope and hydrogen peroxide concentration using hydrogen peroxide strip test, also for its bacterial contents using standardized microbiological culture.Results: The measurements of the water activity, pH level,production of hydrogen peroxide, also the bacterial content, showed that there is no significant difference between the manuka honey and the local ones.Discussion: Both of the honey pH levels are still within the normal range of honey pH level (3.5-4.5) which still have the antibacterial properties to inhibit bacterial growth. Both of the honey water activity level are still within the normal range of honey water activity level (0.500–0.650) which still have the antibacterial properties to inhibit bacterial growth. Manuka honey has been known as non peroxide honey that didn’t produce any hydrogen peroxide.Conclusion:Indonesian local honey which has been used in this research has a similar physical–chemical characteristic as Manuka honey; making it a very good alternative source of medicinal honey because of its affordability and availability.