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All Journal Journal Syifa Sciences and Clinical Research (JSSCR)
Adam Mustapa, Muhammad
Universitas Negeri Gorontalo
Chemistry Medicine & Pharmacology

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IDENTIFIKASI ISOLAT KULIT BATANG WARU (Hibiscus tiliaceus L.) MENGGUNAKAN SPEKTROSKOPI INFRAMERAH Taupik, M.Sc, Muhammad; Adam Mustapa, Muhammad
Journal Syifa Sciences and Clinical Research Vol 1, No 1 (2019): March
Publisher : State University of Gorontalo

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Abstract

Waru (Hibiscustiliaceus L.) bark is a plant that is believed and used by community as a traditional medicine in treatment, especiallytotreat fever. This study aims to analyze the level of a secondary metabolite compound in the bark of waru (Hibiscustiliaceus L.) plant calculated using UV-Vis spectrophotometry and infrared methods. The method used to identify the flavonoid content is Thin Layer Chromatography (TLC) using eluent n-hexane and ethyl acetate at the best comparison (7:3). The result obtained from this study is waru bark extract containing flavonoid compound which is shown from the Rf value of the waru bark methanol extract of 0.82, which the value is close to the Rf value of quarcetine 0.83. Analysis of the flavonoid content of waru bark methanol extract has carried out on The Spectrophotometric UV-Vis at a wavelength of 382 nm and the total flavonoid content obtained is of 10 mg of waru bark methanol extract containing135.2166µg/mL flavonoid compound with percentage of 13.521% and on the Spetroscopy Infrared, based on the obtained peak shows the existence of functional groups of OH, CH aliphatic, C=C aromatic and C-O indicates that this isolat is a flavonoid compound.
ANALISIS KADAR FLAVANOID TOTAL MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS DALAM KULIT BUAH SALAK (Salacca zalacca V.) Adam Mustapa,M.Sc, Mohammad; Taupik, Muhammad; Ramadhan L, Aditya
Journal Syifa Sciences and Clinical Research Vol 1, No 1 (2019): March
Publisher : State University of Gorontalo

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Abstract

The Snake Fruit (Salacca zalacca (Gaertn.) Voss) skin is considered as a waste, but there are a small number of people who use this fruit skin as an medicine. A compound that plays a role in snake fruit skin for the treatment is flavonoid. The purpose of this study was to determine the level of flavonoid contained in the extract of snake fruit skin. The method used to determine the amount of flavonoid compound contained in it is the UV-Vis Spectrophotometry method. The result of reading the absorbance value of each snake fruit fraction obtained from the methanol fraction was 0.568 and ethyl acetate fraction of 0.319, which were then calculated by using the quercetin linear equation standard, y = 0.0608 x -0.0188 with the correlation coefficient (R2) = 0.997. The result obtained for each snake fruit fraction for flavonoid level in the methanol fraction was 96.51 µg/mL and in ethyl acetate fraction was 55.56 µg/mL.
ANALISIS KADAR FLAVANOID TOTAL MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS DALAM KULIT BUAH SALAK (Salacca zalacca V.) Adam Mustapa,M.Sc, Mohammad; Taupik, Muhammad; Ramadhan L, Aditya
Journal Syifa Sciences and Clinical Research Vol 1, No 1 (2019): March
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (547.013 KB)

Abstract

The Snake Fruit (Salacca zalacca (Gaertn.) Voss) skin is considered as a waste, but there are a small number of people who use this fruit skin as an medicine. A compound that plays a role in snake fruit skin for the treatment is flavonoid. The purpose of this study was to determine the level of flavonoid contained in the extract of snake fruit skin. The method used to determine the amount of flavonoid compound contained in it is the UV-Vis Spectrophotometry method. The result of reading the absorbance value of each snake fruit fraction obtained from the methanol fraction was 0.568 and ethyl acetate fraction of 0.319, which were then calculated by using the quercetin linear equation standard, y = 0.0608 x -0.0188 with the correlation coefficient (R2) = 0.997. The result obtained for each snake fruit fraction for flavonoid level in the methanol fraction was 96.51 g/mL and in ethyl acetate fraction was 55.56 g/mL.
IDENTIFIKASI ISOLAT KULIT BATANG WARU (Hibiscus tiliaceus L.) MENGGUNAKAN SPEKTROSKOPI INFRAMERAH Taupik, M.Sc, Muhammad; Adam Mustapa, Muhammad
Journal Syifa Sciences and Clinical Research Vol 1, No 1 (2019): March
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (663.495 KB)

Abstract

Waru (Hibiscustiliaceus L.) bark is a plant that is believed and used by community as a traditional medicine in treatment, especiallytotreat fever. This study aims to analyze the level of a secondary metabolite compound in the bark of waru (Hibiscustiliaceus L.) plant calculated using UV-Vis spectrophotometry and infrared methods. The method used to identify the flavonoid content is Thin Layer Chromatography (TLC) using eluent n-hexane and ethyl acetate at the best comparison (7:3). The result obtained from this study is waru bark extract containing flavonoid compound which is shown from the Rf value of the waru bark methanol extract of 0.82, which the value is close to the Rf value of quarcetine 0.83. Analysis of the flavonoid content of waru bark methanol extract has carried out on The Spectrophotometric UV-Vis at a wavelength of 382 nm and the total flavonoid content obtained is of 10 mg of waru bark methanol extract containing135.2166g/mL flavonoid compound with percentage of 13.521% and on the Spetroscopy Infrared, based on the obtained peak shows the existence of functional groups of OH, CH aliphatic, C=C aromatic and C-O indicates that this isolat is a flavonoid compound.
Co-Authors Muhammad Taupik Ramadhan L, Aditya