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Analisis Selektivitas Senyawa Turunan Diosmetin Sebagai Antioksidan Baru dengan menggunakan Metode MolecularDocking Martati, Titiek; Mumpuni, Esti; Mulatsari, Esti; Maryanto, Kenny
Jurnal Farmasi Indonesia Vol 10, No 1 (2018)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1421.953 KB) | DOI: 10.35617/jfi.v10i1.581

Abstract

Free radicals are compounds that have nofree electron pair, so it ws unstable and too reactive, to be able to ward off free radicals, required an antioxidant. Antioxidant can prevent the occurrence of oxidative reactions that can cause various diseases. The commonly used antioxidant compounds are vitamin C, vitamin E, and flavonoids. One of flavonoid compound that have the potential to be developed into antioxidants is diosmetin. The results of the study using the QSAR method, state that some of diosmetin derived compounds have better antioxidant activity than diosmetin. In this research, the selectivity of diosmetin derived compounds in several enzymes as an antioxidant was tested by using molecular docking methods. Software used for molecular docking were PLANTS, YASARA, MarvinSketch. This study used 15 diosmetin derived compounds, diosmetin as a parent compound, and comparison compounds used were vitamin C, vitamin E, and quercetin. Validated target proteins are 7 (seven) receptors with PDB codes 1QQW, 1V4S, 1XAN, 2BEL, 2C9V, 4K7O, and 5M2F. The results of this study have obtained the best selective compounds to receptors and compounds in each receptor. The best compounds are 6,8-difluoro diosmetin (1QQW), 8-amine diosmetin (1V4S), 6.8-difluoro diosmetin (1XAN), 6.8-diamine diosmetin (2BEL), 5'-amine diosmetin ( 2C9V), 5'-amine diosmetin (4K7O), and 8-amine diosmetin (5M2F). Free radicals are compounds that have nofree electron pair, so it ws unstable and too reactive, to be able to ward off free radicals, required an antioxidant. Antioxidant can prevent the occurrence of oxidative reactions that can cause various diseases. The commonly used antioxidant compounds are vitamin C, vitamin E, and flavonoids. One of flavonoid compound that have the potential to be developed into antioxidants is diosmetin. The results of the study using the QSAR method, state that some of diosmetin derived compounds have better antioxidant activity than diosmetin. In this research, the selectivity of diosmetin derived compounds in several enzymes as an antioxidant was tested by using molecular docking methods. Software used for molecular docking were PLANTS, YASARA, MarvinSketch. This study used 15 diosmetin derived compounds, diosmetin as a parent compound, and comparison compounds used were vitamin C, vitamin E, and quercetin. Validated target proteins are 7 (seven) receptors with PDB codes 1QQW, 1V4S, 1XAN, 2BEL, 2C9V, 4K7O, and 5M2F. The results of this study have obtained the best selective compounds to receptors and compounds in each receptor. The best compounds are 6,8-difluoro diosmetin (1QQW), 8-amine diosmetin (1V4S), 6.8-difluoro diosmetin (1XAN), 6.8-diamine diosmetin (2BEL), 5'-amine diosmetin ( 2C9V), 5'-amine diosmetin (4K7O), and 8-amine diosmetin (5M2F). Free radicals are compounds that have nofree electron pair, so it ws unstable and too reactive, to be able to ward off free radicals, required an antioxidant. Antioxidant can prevent the occurrence of oxidative reactions that can cause various diseases. The commonly used antioxidant compounds are vitamin C, vitamin E, and flavonoids. One of flavonoid compound that have the potential to be developed into antioxidants is diosmetin. The results of the study using the QSAR method, state that some of diosmetin derived compounds have better antioxidant activity than diosmetin. In this research, the selectivity of diosmetin derived compounds in several enzymes as an antioxidant was tested by using molecular docking methods. Software used for molecular docking were PLANTS, YASARA, MarvinSketch. This study used 15 diosmetin derived compounds, diosmetin as a parent compound, and comparison compounds used were vitamin C, vitamin E, and quercetin. Validated target proteins are 7 (seven) receptors with PDB codes 1QQW, 1V4S, 1XAN, 2BEL, 2C9V, 4K7O, and 5M2F. The results of this study have obtained the best selective compounds to receptors and compounds in each receptor. The best compounds are 6,8-difluoro diosmetin (1QQW), 8-amine diosmetin (1V4S), 6.8-difluoro diosmetin (1XAN), 6.8-diamine diosmetin (2BEL), 5'-amine diosmetin ( 2C9V), 5'-amine diosmetin (4K7O), and 8-amine diosmetin (5M2F).
Free Radical Scavenging and α-Glucosidase Inhibitor Activity of Ethanolic Extract of Mucuna pruriens L. Widowati, Wahyu; Ratnawati, Hana; Retnaningsih, Ch.; Lindayani, .; Rusdi, Udju D.; Winarno, Wahyu
Jurnal Farmasi Indonesia Vol 5, No 3 (2011)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v5i3.46

Abstract

Biji kacang â??koro bengukâ? atau velvet bean sudah sangat dikenal tetapi dasar ilmiah penggunaannya sebagai obat tradisional terutama sebagai antioksidan dan antidiabetik masih belum diketahui. Oleh sebab itu dalam penelitian ini dilakukan percobaan untuk mengevaluasi aktivitas antioksidan dan antidiabetik dari ekstrak biji koro benguk, serta fraksi heksana, etil asetat, butanol dan air menggunakan uji in vitro menggunakan DPPH (1,1-difenil-2-pikril-hidrazil) dengan pembanding positif kuersetin dan BHA. Hasil penelitian menunjukkan bahwa IC50 DPPH untuk ekstrak biji koro benguk, fraksi heksana, etil asetat, butanol dan air berturut-turut sebesar 22,17; 152,60; 113,03; 4,93; dan 9,14 μg/mL, sedangkan untuk kuersetin dan BHA masing masing sebesar  4,28 dan 22,587 μg/mL. Aktivitas antidiabetik ditentukan menggunakan metode penghambatan α-glukosidase dengan glucobay sebagai kontrol positif. Hasil penelitian menunjukkan bahwa IC50 antidiabetik untuk ekstrak biji koro benguk, fraksi heksana, etil asetat, butanol, air, dan glucobay berturut-turut sebesar 24,44; 19,55; 8,40; 80,98; 48.97; dan 16,23 μg/mL. ABSTRACT Velvet bean seeds are ubiquitous but the scientific basis for its medicinal use especially as antioxidant and antidiabetic remains unknown. Therefore, a study was designed to evaluate  antioxidant and antidiabetic activities of the  extract, hexane, ethyl acetate, butanol and water fractions of velvet bean seeds. In order to evaluate antioxidant activity of extract and fractions  using  quercetin,  butylated hydroxyanisole (BHA) as positive control and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity was determined. The results showed that IC50 of DPPH were 22.17; 152.60; 113.03; 4.93; 9.14 μg/mL respectevily;  quercetin 4.28 and BHA 22.587 μg/mL.  Antidiabetic activity was determined by using  of α-glucosidase inhibition assay of  extract, four fractions and glucobay as positive control. The results showed that antidiabetic IC50 were24.44; 19.55; 8.40; 80.98; 48.97μg/mL respectively and glucobay as positive control  16.23 μg/mL.
Co-Authors . SUKRISTIYONUBOWO A.A Ngurah Narendra A.T. Aman Abdul Basid Abdul Latief Abadi Abdul Waris Adi Sucipto, Adi Adli, Jeri Adna Sumadikarta ADRIANI ADRIANI Afrizal . Agustina Merdekawati Ahmad Ahsin Kusuma Mawardi Ahmad Fauzi Habiba Chozin Ahmad Marzuki Ahmad Yani T Ali Agus Ali Djamhuri Alimuddin Laapo Alma Pratiwi Alpandari, Heny Amalia Putri Firdausi Amelia Widiatuti Amin Suyitno Ana Majdawati Anak Agung Gede Sugianthara Ancah Caesarina Novi Marchianti Andralia Mayangsasati Andrean Dikky Pradhana Putra Andri Purwandari Angelia Angelia Angelina Widya Gustina Angga Ade Sahfitra Anin Ditto Anita Hidayati Anton Nugroho Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Ardiana Ardiana, Ardiana Ardiansyah, Rifky Ari Kamayanti Arie Febiansyah Pasaribu Arief Boediono Ariffin Noor Sugiharto Arini Risqi Adisti Aris Triwiyatno Arista, Yeli Arlina Dewi Atik Andriana Ayudianingrum, Asti Azizah, Elvira B. E. F Da Silva Bagus Putu Yudhia Kurniawan Baiq Retno Sofiana Iqsir Baiq Rosyida Bambang Darmo Yuwono Bambang Santoso Bambang Suhartanto Bambang Suwignyo Basri, Amelia Ruwaida Benito Heru Purwanto Berti Yolida Bq. Anggun Hilendri Budiarjo, Budiarjo Bustami, Mohammad Reevany Cahyaningtyas, Susi Retna Candra Setyawati, Candra Cari, Cari Ch. Retnaningsih, Ch. Chichik Nirmasar Claudy, Chintya Crismawati, Crismawati D. SANTOSO D. Tialurra Della Nabila Dafina Howara Dahyar Daraba Dali, Dali Dana Indra Sensuse Darsikin Darsikin Dasmen, Rahmat Novrianda Daviq Chairilsyah Dede Marina Department, administratio Desi Areva Desman Hidayat, Desman Desri S Sipaga, Desri S Dewi, Fransisca Christiana Dharmawan Santoso Dian Syafitri Ompusunggu Dianita Sugiyo, Dianita Djaswadi Dasuki Djohar, Setiadi Dwi Wahyu Setiyarini E. TUHERKIH Eddiwan Kamaruddin Eddy Suparman edi sumantri Een Suryani Efawani Efawani Eka Tarwaca Susila Putra Eko Hanudin Elfa Rafulta - Elin Erlina Sasanti Elin Erlina Sasanti Elisa Elisa Emalisa Emalisa Endang Sugiharti, Endang Erna Johan Ernawati Ernawati Erniati Erniati Esti Mumpuni, Esti Etfita, Fauzul Eucenda Erwin P. Eva Melinda Evan Evan Faik Kurohman Fajar Basuki Fajri, Nur Farhan Firdiansyah Farida Ariyani Farida Fathul Farida Ilmah, Farida Fatoni, Fatoni Fefri Indra Azra Fendra Wician Firmansyah - - Fitra, Halkadri Fitrah Satrya, Fitrah Fitri Diani Fitri, Duane Ayu FX Sri Sadewo Galaresa, Achmad Vindo Galaresa, Achmad Vindo Gatot Suharto Abdul Fatah Gatot Yulianto Grizmaldy, Fastri Prisma H. Fuad Nashori Hadi Nasbey Haifa Victoria Silitonga Hamid, Marsud Hanafi Hanafi Hanan Lanang D. Haniarta Gemilang Sari HANNA ARTUTI EKAMAWANTI Hans Hernando Hardy Primadi HARI PURNOMO Hariadi, M. Helmi Harifuddin Harifuddin, Harifuddin Harjudin, Laode Harsono Harsono Hartono Gunarso Hasannudin, Hasannudin Hasman Hasyim Hata Maulana Hayati, Paramidita Nurul Hayatunnufus Hayatunnufus Herbert . Hermini Susiatiningsih Heru Kurnianto Tjahjono Heru Kuswanto Hildawati S, Hildawati S Hilendri, Bq. Anggun HM Mozard B Darus Husna, Putri Halimu I GEDE PUTU WIGENA I Made Aditya Prabawa I Made Bayu Wisnawa I MADE JEMBER I Made Oka Widyantara I MADE SURYANA . I N Satya Kumara I'tishom, Reny Ida Bagus Nyoman Mantra Ida Purniawati, Ida IKA APRILIA Imam Triarso Iman Waskito Imelda . Indah Cahya Dewi Indah Permata Sari Indriani Kurniadi Indriyenni, Indriyenni Insih Wilujeng Intan Rakhmawati Irfan Sofiyan Irfan Syauqi Beik Irma Wardati Irmayanti, Desy Isfiyanto, Isfiyanto Ita Fauzia Hanoum, Ita Fauzia Ita Riniatsih Izwerni Izwerni Jayanti, Jayanti Jayanti, Jayanti Jesika Setiawan Joko Sulistyo Joni Ennervon Taraja Jovita Jovita Julianti, Tika Jumadi Jumadi Juni Hestina Jusman Mansyur Kanya Damarçanti Karman Lanani Kasman Rukun Khaerul Wahidin Khulatul Bariroh Kristian Ariya Sedayu Kusdhiarningsih, Betty Kusdiantomo Kusdiantomo Kusumowidagdo, Gladys Kusworo Anindito L. Takdir Jumaidi Laksmono, Bambang Shergi Laksono Trisnantoro Laraswati Laraswati Lellee, Ralph. J. Lenap, Indria Puspitasari Lestari, Vitri Liani, Evi Lily Thamrin Lindayani, . Lisa Khairiah Damanik Listyani, Refti Handini Liza Novitha Sari Luhut Sihombing Lukman Effendy Lukman M. Baga Luqman Qurata Aini Lusi, Lusi Luwiyanto, Luwiyanto M. Akbar Husein Allsabah M. Ali Fikri M. Ali Fikri M. Fa’iq Azhari Ma'mun Sarma Made Sudarma Made Yos Kresnayana Mahima, Anugrah Mahmud Mulyadi Mahyudin Mahyudin Marhamah Marhamah Marshell Tendean Maryanto, Kenny Maryati Maryati Marzuki Marzuki masra dona fitri Matiur, Eiko B. Mawarni, Siti Maya Damayanti Maya Damayanti Megawati Simanjuntak Melianti, Eka Oktaviani Moh Afandi Moh. Ismail MOH. MUDZAKKIR Mondra Neldi Monica Puspa Sari Mudinillah, Adam Mufrida Meri. Z Muhammad Anwar Muhammad Azka Maulana Muhammad Faisal AR Pelu Muhammad H Q Muchransyah Muhammad Hafiun, Muhammad Muhammad Haikal Kautsar Muhammad Hasbi Muhammad Jufri Muhammad Raihan Aufar Muhiddin Palennari Mukhlis Mukhlis Mulatsari, Esti Mulkan Iskandar Nasution Mulyadi Mulyadi MULYOTO PANGESTU Murni Astuti Mursid Rahardjo Musadad, Arif Musharyanti, Lisa Mustaring Mustaring Musthofa Lutfi Mutmainah, Dini Nafisatul N. Surya Gunawan Nafiatul Umami Naila Izzah Najamuddin, Suhartina Najoan N Warouw Napitupulu, Sabar Nasrullah Nasution, Atiqi Chollisni Nendri, Nendri Nengah Dwi Handayani Ni Gst. Ag. Gde Eka Martiningsih Ni Made Dwi Puspitawati Ni Made Suriani Ninik Umi Hartanti Novita Yanti Sikumbang Noviyarsi Novpi Susanto Nugrahani, Fitria Nungki Kartikasari Nunuk Suryani Nurbani Yusuf Nurfaedah Nurjanah, Fitri Nurlaksana Eko Rusminto Nurul Hikmah Octasella Ainani As’ad Oka Saputra Oktaviana, Shinta Oktaviana, Shinta Perdana, Ridho Putra Popy Purnamasari Wahid Suyitno, Popy Purnamasari Wahid Pradiptyas, Ayres Pratiwi Nurhascaryani PUDJI ASTUTI Purwandari, Kristiana Puji Purwanty, Ratna Puspitasari Puspitasari Putri Yulianti, Nel Ayu Putu Agus Prayogi Quinoza Guvil - Radia Purbayati Rahayu, Semmy Damarjatie Rahmah, Fathatur Rahmanta Ginting Rahmi Sri Ramadhani Rakhmawati, Intan Ramces Sitohang Ratna Sitompul Ratna Winandi Regie Santoso Renata Anggraeni, Renata Reryd Arindany Wiryawan Respati Mahadhika Rezza Abdurrahman Ibnu Aziz Ria Azizah Tri Nuraini Richardus Eko Indrajit Riki Muhamanda Rini Rita T Marpaung Risa Panti Ariani Ristianto Utomo Ristiawan Agung Nugroho Ristya Widi Endah Yani Rita Nurmalina Ritna Udiyani Riva Ismawati, Riva Rivo Panji Yudha, Rivo Panji Rizal Rinaldy Rohma, Fatona Fadilla Rometdo Muzawi, Rometdo Ronald ., Ronald Rosalina, Linda Rosari, Renya Rostamailis Rostamailis Rosyida, Baiq Rr. Rina Antasari Rr. Sri Kartikowati Rudi Hartono Rudy Sutrisna Rusdin Rusdin Ruslan . Rut CS Siahaan S.H. Purwanto Saefudin Saefudin Sahara Sahara Sahat Siagian Saiful Bahri Saipul Arni Muhsyaf Saipul Arni Muhsyaf Saleha Sungkar Salmah Orbayinah Salmiah Salmiah Santi Nurhasanah Sapto Indrioko Saptoyo Argo Morosidi, Saptoyo Argo Saputra, Marcalleno Reza Sarah A E Retraubun Sari, Della P. Sari, Hastuti Indra Sari, Risna Sawitri Subiyanto Shofwal Widad SIGIT SANJAYA, SIGIT Sinar Perbawani Abrina Anggraini Siti Atikah Siti Atikah Siti Atikah Siti Khadijah Nasution Sitti Hadija, Sitti SLP Dawisni Manik Pinatih Son haji, Son Sonia Ramadhani Hutasuhut Sri Lestariningsih, Wahyuni Sri Mulatsih Sri Nita - Sri Nuryani Hidayah Utami Sri Nuryani Hidayah Utami Sri Utami Dewi SRIANA WASTI Sudjadi Sudjadi Sugiarti Sugiarti Sugiarto S Sugiatno Sugiatno Sugiyono Sugiyono Suhaedi, Wirawan Sukatik - - Sularno Sularno, Sularno Sulastri Sulastri SUMIYATI SUMIYATI Sunanda Naibaho Supriyatiningsih, Supriyatiningsih Surjadi Sujana Suryandari, Galuh Susan Susana Chandra Susanna Ndraha Susi Retna Cahyaningtias Sutiyo Sutiyo Suwarto, Sigit Suyadi . Suyoto Suyoto Suzanna Ndraha Swesti Theresia, Swesti Syafar, Andi Kodratul Syamaun A. Ali Syamsul Bahri Yusuf Syamsuri Rahim T.Z. Helmi Taamu, Taamu Taty Sulastri Taufik Akbar Taufik Iskandar Teguh Suroso Theresia Devi Indriasari Tias Pratama, Tias Tita Nursyamsiah, Tita Titin Setyorini, Titin Tjokorda Istri Praganingrum Togar Fernando Manurung Tri Arief Sardjono Tri Eko Purwanto Tri Wulandari Kesetyaningsih Tria Harsiwi Nurul Insani Triandi Kiansyah Trisakti Handayani Tuwu, Darmin Udin Sidik Sidin Udju D. Rusdi Unggul Wahyono V.A Irmayanti Harahap Vita Purnamasari Vitria Puri Rahayu Wahidatul Husnaini Wahyu Ismail Kurnia WAHYU WIDOWATI WAHYU WINARNO Wahyuni, Dedek Wardani, Indah Kusuma Wati, Kandiana Wenny Artanty Nisman, Wenny Artanty Wenny Savitri, Wenny Widdiyanti, Widdiyanti Widyawati Widyawati Wildati Zahri Wilhelmus Wolo Winaruddin Winaruddin Wini Tarmini Wira Meswari, Sisi Yulia Wong Hendra Widjaja Wospakrik, Frengki Wulandari Wulandari Y. Pranoto Yafi Sabila Rosyad Yahya Hairun Yahya Yahya Yandraini Yunida - Yani Cahyo Susilo Yeni D. Lestari, Yeni D. 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