Rotinsulu, Dordia Anindita
Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor

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Seroprevalensi dan Faktor Risiko Rabies pada Anjing di Daerah Endemis Kabupaten Sukabumi Ardilasunu Wicaksono; Abdul Zahid; Etih Sudarnika; Dordia Anindita Rotinsulu; Denny Widaya Lukman; Sri Murtini; Yusuf Ridwan
Jurnal Ilmu Pertanian Indonesia Vol. 26 No. 3 (2021): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.26.3.436

Abstract

Sukabumi District is reported to date as an endemic rabies area in West Java Province. In supporting the rabies control programs, the occurrence of rabies needs to be determined to decrease the number of cases. This research aims to determine the seroprevalence of rabies and identify the risk factors associated with rabies in the district. The research was conducted in Jampang Tengah and Cisolok sub-districts with positive rabies cases on dogs and humans. The blood samples were taken from 191 selected dogs using simple random sampling. Rabies antibody titer was measured using ELISA, and the risk factor was identified using selected dogs’ data classified into intrinsic and extrinsic characteristics. Data were analyzed using the chi-square test and the calculation of the odds ratio. The results showed that the seroprevalence of rabies was 7.9% (4.9-10.9%). The average antibody titer was 0.23 EU with a minimum value of 0.03 EU and a maximum of 0.96 EU. Thus, as high as 7.8% of the population showed rabies seropositivity from the unvaccinated dogs, while among the vaccinated dogs, only 8.0% of the population with rabies protective antibody titer. The significant risk factor of rabies seroprevalence is the typology of raising area (p = 0.003), revealing that the dogs raised in the coastal area had the odds of 5.8 (1.6-21.2) higher to get the rabies seropositivity than the dogs raised in the highland area. Keywords: antibody titre, dogs, rabies, risk factor, seroprevalence
Seroprevalensi Rabies Pascavaksinasi pada Populasi Anjing di Kawasan Endemik Rabies di Kabupaten Sukabumi Sri Murtini; Dordia Anindita Rotinsulu; Yusuf Ridwan; Denny Widaya Lukman; Abdul Zahid; Ardilasunu Wicaksono; Usamah Afiff; Asah Hilaliah; Risqika Akla Velayati; Etih Sudarnika; Koekoeh Santoso; Agus Wijaya
Acta VETERINARIA Indonesiana Vol. 10 No. 1 (2022): Maret 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.1.87-95

Abstract

Penelitian ini bertujuan untuk mengevaluasi hasil vaksinasi rabies dan membandingkan kenaikan titer antibodi yang terbentuk di Kecamatan Cisolok dan Jampang Tengah sebagai Kawasan endemik rabies Kabupaten Sukabumi dan mengasosiasikan titer antibodi yang terbentuk dengan umur, jenis kelamin, dan cara pemeliharaan anjing yang diperiksa . Sebanyak 211 anjing diamati pada studi ini. Sampel serum prevaksinasi dan pascavaksinasi diambil dan selanjutnya diuji dengan uji ELISA tidak langsung untuk mengetahui titer antibodinya. Data titer antibodi dari masing-masing sampel dianalisis secara deskriptif, sedangkan asosiasi umur, jenis kelamin, dan cara pemeliharaan dengan titer antibodi yang terbentuk pascavaksinasi dihitung menggunakan uji chi-square. Hasil pengujian menunjukkan seroprevalensi rabies di Kecamatan Cisolok dan Jampang Tengah sebelum vaksinasi adalah 17,3% dan 2,6% dengan titer 0,58 EU/mL dan 0,55 EU/mL, sedangkan seroprevalensi setelah vaksinasi menjadi 62,5% dan 93% dengan titer 1,29 EU/mL dan 1,33 EU/mL. Variabel umur, jenis kelamin, dan cara pemeliharaan tidak berasosiasi nyata dengan peningkatan titer antibodi rabies setelah vaksinasi. Hasil studi menyimpulkan bahwa seroprevalensi rabies di kawasan endemik rabies di Kabupaten Sukabumi meningkat setelah dilakukan vaksinasi dari sebelumnya 11% menjadi 75,8%, dengan nilai peningkatan titer (Δ titer) sebesar 1,09 EU/mL.
Perbandingan Deteksi Titer Antibodi Pascavaksinasi Rabies Berbasis Kolorimetri Menggunakan ELISA Reader dan Kamera Telepon Genggam Koekoeh Santoso; Ulfatin Khoiriyah Herowati; Dordia Anindita Rotinsulu; Sri Murtini; Muhammad Yusuf Ridwan; Denny Widya Hikman; Abdul Zahid; Ardilasunu Wicaksono; Arifin Budiman Nugraha; Usamah Afiff; Agus Wijaya; Ridi Arif; Ronald Tarigan; Edi Sukmawinata
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.315 KB) | DOI: 10.19087/jveteriner.2021.22.1.79

Abstract

Rabies is an infectious disease, zoonotic, caused by virus from the genus Lyssa virus and generally transmitted by the bite of rabid animal, especially rabies infected dog. Rabies is preventable but is always fatal to humans if the central nervous system (CNS) is infected. Vaccination has been used as one of rabies prevention programmed. A total of 83 samples were tested using an Indirect ELISA (Enzyme Linked Immunosorbent Assay) to identify post-vaccination rabies antibody titer. Antibody titres correlated with absorbance values and standard solutions concentrations. Absorbance value can be determined using ELISA reader and mobile phone camera. Absorbance were read at 450 nm and 620 nm as reference using ELISA reader and image from mobile phone camera using image processing software (ImageJ). The aim of this study is to compared between ELISA reader as gold standard and mobile phone camera through validity testing such sensitivity, specificity, and accuracy. There is no significant difference between gold standard and alternative test equipment. The mobile phone camera has sensitivity 98,6%, specificity 88.8 % and accuracy 97,5%. The image processing method using ELISA reader is relatively expensive and difficult to hold in laboratory with minimum funds. Image processing method using a mobile phone camera with ImageJ application is expected to be an alternative tool to read the result of ELISA.
Efektivitas antimikroba terhadap Pasteurella multocida dan Mannheimia haemolityca dari sapi yang diduga menderita bovine respiratory disease kompleks Maria Luisa MNB Klobongona; Usamah Afiff; Dordia Anindita Rotinsulu
ARSHI Veterinary Letters Vol. 3 No. 2 (2019): ARSHI Veterinary Letters - Mei 2019
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.794 KB) | DOI: 10.29244/avl.3.2.39-40

Abstract

Penelitian ini bertujuan untuk mempelajari efektivitas beberapa jenis antibiotik terhadap Pasteurella multocida dan Mannheimia haemolityca dari sapi yang diduga menderita Bovine Rsepiratory Disease complex (BRD complex). Isolat diperoleh dari paru-paru sapi. Identifikasi bakteri menunjukkan adanya bakteri yang umum terlibat pada kasus BRD complex  seperti P. multocidadan M. haemolityca. Uji sensitivitas antimikroba dilakukan terhadap tujuh antibiotik (bacitracin, vancomicin, oxytetracycline, enrofloxacin, gentamicin, ampicillin, dan erythromycin) menggunakan metode Kirby-Bauer dengan mengukur diameter zona hambat. Interpretasi standar yang digunakan didasarkan pada Clinical Laboratory Standards Institute (CLSI). Hasil penelitian menunjukkan bahwa isolat P. multocida sensitif terhadap enrofloxacin, oxytetracycline, gentamicin, dan ampisilin; resisten terhadap bacitracin dan vankomisin; ke eritromisin. Sedangkan isolat M. haemolityca sensitif terhadap enrofloxacin dan gentamicin; resisten terhadap bacitracin, vankomisin, oksitosin ampisilin, dan eritromisin