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Use of pregnant mare’s sera gonadotropin (PMSG) in media in vitro maturation of cow oocytes Udin, Zaituni; ., Jaswandi; Afriani, Tinda; E, Leonardo
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.149 KB) | DOI: 10.14334/jitv.v12i1.564

Abstract

It is known that hormone addition in media helps in vitro maturation of oocyte. This research was aimmed to determine the effect of PMSG in media to maturation rate and nucleous developvement of cow oocyte. Ovaries were obtainned from local slaughterhouse. The media used for in vitro maturation of oocyte was TCM- 199 and the treatment was 3 levels of PMSG: 0, 10 and 20 mg/ml. Result of this research showed that the dose of PMSG in maturation media was significantly affected (P<0.05)  nucleolus development of oocytes and maturation rate. The average of germinal vesicle (GV) stage in 3 levels of PMSG 0, 10 and 20 mg/ml were 38.33; 12.64 and 9.64%, respectivelly. There was no germinal vesicle breakdown (GVBD) found in 3 levels of PMSG addition. The nucleous development of metaphase–I (M-I) were 7.64; 20.2 and 22.00%, but the average of maturation rate (M-II)  was 16.32; 48.10 and 35.34% for 3 levels of PMSG: 0, 10 and 20 mg/ml, respectivelly. It is concluded that 10 mg/ml PMSG in media of in vitro maturation resuls in the highest maturation rate of cow oocyte. Key Words: Oocyte, Maturation, In vitro, Hormone, PMSG
The Manipulation effectivity of cell co-cultures in 5% CO2 incubation system to increase in vitro cattle embryo production Syaiful, Ferry Lismanto; BP, Zesfin; Saladin, R; ., Jaswandi; ., Hendri
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 1 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.437 KB) | DOI: 10.14334/jitv.v15i1.678

Abstract

The purpose of this research is to determine the effectivity of various cell co-cultures of cattle embryo production by in vitro CO2 5% incubation system and get the best cell co-culture. Cell co-culture which are used in the synthesis is the oviduct cells, isthmus cells, ampulla cells, follicle cells and without cells. Data were analyzed based on completely randomized desiggn. The average growth rate/ cleavage in various cell culture was: the oviduct cell 59.24%, ampulla cell  58.69%, isthmus cell 58.25%, follicle cell 52.24% and without cells 47.76%. The average  growth of 8-16 cells embryos to various cell co-culture was: the oviduct cell 46.02%, ampulla cell 45.45%, isthmus cell 45.15%, follicle cell 43.07%, and without cell 38.50%. The mean percentage of morula in various cell co-culture treatment was: the oviduct cell 20.59%, ampulla cell 20.48%, isthmus cell 20.30%, follicle cell 16.96% and without cell 12.58%. The average percentage of embryonic growth (cleavage, 8-16 cells and morula) was not significantly different (P > 0.05).  The treatment of a variety of cell co-culture increased significantly (P>0.05), blastocysts production, namely: the oviduct cell 3.28%, ampulla cell 3.22%, isthmus cell 3.08%, follicle cell 2.45% and without cell 1.97%.  In conclusion, the treatment of various cell co-culture in 5%CO2   incubation system can increace the growth of cattle embryos in vitro.   Key words: Cell Co-Culture, In Vitro Embryo, 5%CO2 Incubation System, Cattle
Post-partum reproductive appearance of buffalo at various levels of GnRH and synchronized with PGF ., Yendraliza; Zespin, B.P; Udin, Z; ., Jaswandi
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 2 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.819 KB) | DOI: 10.14334/jitv.v17i2.684

Abstract

A study of reproductive performance of buffalo treated with levels of GnRH was conducted on twenty buffaloes of Kampar. The animals were divided into five groups. Group 1 received 200 µg GnRH, the second group received 250 µg GnRH, the third group received 300 µg GnRH, the fourth group received 350 µg GnRH and the fifth group received 400 µg GnRH. Each group also received 12.5 µg PGF2α at days 7 following GnRH injection. Sign of estrus was observed 9 days after GnRH injection. Statistical analysis was performed based on completely randomized design. Results showed that the injection of 300µg of GnRH that was followed by 12,5 µg of PGF2α was able to show obvious sign of post-partum oestrus, with sign of estrus appeared at oestrus rate of 27.8 hours, following PGF2α injection, with 100% of pregnancy rate and 100% of calving rate. Key Words: Estrus, Pregnancy Rate, Calving Rate
STUDY ON NUTRITION OF EGGS JELAWAT (Leptobarbus hoeveni Blkr) Aryani, Netti; Zen, Zuhelmi; Syandri, Hafrijal; Jaswandi, Jaswandi
Jurnal Perikanan dan Kelautan Vol 14, No 01 (2009)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.14.01.%p

Abstract

The purpose of study is to know nutrition of jelawat eggs, compriseprorein, fat, calcium, phospore, essential amino acid, essential fatty acid, vitamineC and vitamine E. Method applied to get the purpose is take jelawat eggshave TKG IV ( gonad stages mature) on brood stock with 4kg, and weight wet ofgonad 600 gram at Maninjau Lake. Eggs are dried and floured. The study usesproximate-analysis to get quantity of protein, fat, calcium and phospore andHPLC methode to get the essential amino acid, fatty acid and vitamine C and E.The result show that fish jelawat floured have within it nutrition protein61,33 %, fat, 11,90 %, water content 4,88 %, ashes content 8,26 %. Quantityamino acid 52,69 %, linoleat fatty acid 7,71.%, linolenat fatty acid 0,05 %,vitamine C 0,240 % and vitamine E 0,310 %.
Studi Nutrisi Buah Ara (Ficus racemosa L.) untuk Pakan Ikan Aryani, Netti; Zen, Zuhelmi; Syandri, Hafrijal; Jaswandi, Jaswandi
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (84.384 KB) | DOI: 10.31258/jnat.12.1.54-60

Abstract

The purpose of the research was to find out the countent of nutrition of Ara (Ficus racemosa L) wich covers : thelevel of protein, fat, water, ash, fibrous part of the fruit, calcium, phosphor, vitamine C and E as well as amino acidwhich are very useful for enrichment of fish diets. The sample of Ara fruit were collected directly in the field. Therewere two types of processing Ara fruits used in this research, namely (1) cutting the fruits into thin layer and madeit into; (2) separating the fuit from the seed, dried, and made it into flour. The level of protein, fat, fibrous part of thefruit, water, and ash was analyzed by using proximate analysis, while the level of calcium was analyzed by usingAAS methods. The level of phosphor was measured by using spectrophotometer, the level of vitamine C and E wasanalyzed by using HPLC and amino acid by gas chromatography (CG). The study found that the flesh flour of Arafruit contained protein (10,63%), fat (8,66%), water (7,08 %), ash (11,57%), fibrous part of the fruit (16,15%), calcium514,88 mg/100 g), phosphor (418,29 mg/100 g), vitamine C (475,38 mg/100 g), and amino acid (3,013 %) higher thanAra flour with protein (8,98 %), fat (6,57%), water (10,26%), ash (18,23%), fibrous part of the fruit (20,31%), calcium(0,039 mg/100g), phosphor (0,002 mg/100g), vitamin C 25,48 mg/100 g ), and amino acid (1,24%). It can be concludedthat the Ara flour can be used as on of the ingredients for fish diets especially as the source of vitamine C, calciumand phosphor to incrase reproduction ability and growth.
EFFECT OF COMBINATION OF GnRH AND PGF2α FOR ESTRUS SYNCHRONIZATION ON ONSET OF ESTRUS AND PREGNANCY RATE IN DIFFERENT POSTPARTUM IN SWAMP BUFFALO IN KAMPAR REGENCY Yendraliza, Y.; Zesfin, B.P.; Udin, Z.; Jaswandi, J.; Arman, C.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 1 (2011): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.1.9-13

Abstract

The purposes of this study were to investigate the effect of administration of GnRH and PGF2α ononset of estrus and conception rate in different postpartum swamp buffalo (Bubalus bubalis). Twentypostpartum (pp) buffalo-cows were divided into four groups of five buffaloes as follows: Group A (30day pp), Group B (45 dpp), Group C (60 dpp), and Group D (75 dpp). The buffaloes in each group wereinjected intramuscularly with 3 ml GnRH at day 0, followed by intramuscular injection of 2.5 ml PGF2α,10 ml) at day 7 after GnRH injection. Observation of estrus was performed 24 h after injection of PGF2α.Artificial insemination was performed 18 h after estrus signs clearly visible. The result of study showedthat there were no difference (p>0.05) among treatments on the percentages of estrus (100%), onset ofestrus (37.4 to 38.4 h), duration of estrus (16.8 to 18.2 h) and conception rate (100%). In conclusion,buffalo-cows in Kampar Regency can be served as early as 30 days post partum using combination of 3ml GnRH and 2.5 ml of PGF2α.