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All Journal El-Hayah : Jurnal Biologi Jurnal Teknologi Laboratorium Biosaintifika: Journal of Biology & Biology Education
Prastiyanto, Muhammad Evy
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Medicine & Pharmacology Other

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Antibacterial Activity of Various Extracts of Averrhoa bilimbi against Multidrug Resistant Bacteria Prastiyanto, Muhammad Evy; Wardoyo, Fandhi Adi; Wilson, Wildiani; Darmawati, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23600

Abstract

The multi-drug resistance (MDR) bacteria is a global health problem that causes high mortality every year. Therefore, novel antibacterial agents are needed from natural biological sources. This research aimed to investigate the antibacterial activities of various crude extracts of Averrhoa bilimbi against MDR bacteria. The antibacterial activity was calculated based on the use agar well diffusion assay and the minimum bactericidal concentration (MBC) using Mueller–Hinton broth in a microdilution method. Bacteria from wells were subcultured using inoculating loop onto a 5% sheep BAP. The best antibacterial activity, calculated as the most widely inhibitory zone and the smallest MBC values. The ethanolic extract showed antibacterial activity against the all MDR bacterial test in the agar well diffusion assay (10-14.5 mm inhibition diameter). The MBC of water extract against ESβL + CR Pseudomonas aeruginosa showed the best antibacterial activity (12.5 mg/mL). The fruit of bilimbi was shown to be potentially developed as antibacterial agents, especially for MDR strains. Further in vivo research and discovery of action mode are needed to shed light on their antibacterial effects. This study can provide new information about the benefits of bilimbi as a source of natural antibacterial againts MDR-bacteria
In-vitro antibacterial activity of the seed extract of three member Artocarpus towards methicillin resistant Staphylococcus aureus (MRSA) Prastiyanto, Muhammad Evy; Azizah, Inas Hasna; Haqi, Hafizha Dara; Yulianto, Bagus Dwi; Agmala, Aulia Bella; Radipasari, Zhafira Dias; Astuti, Nita Ayu Dwi; Putri, Arifani Rahma
Jurnal Teknologi Laboratorium Vol 9 No 2 (2020): (In Progress)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i2.237

Abstract

Methicillin-Resistant Staphylococcus aureus (MRSA) infections have created a critical need for the development of natural antibacterials from biological sources. This research aimed to investigate the antibacterial activity of the seed extract of three-member Artocarpus (Artocarpus heterophyllus, A. champeden and A. camansi) against MRSA which are the most prevalent causes of infections in patients. Crude seed extracts of three-member Artocarpus were evaluated for their antibacterial activity against MRSA. The antibacterial activity against MRSA of the three extracts was assayed in vitro by the agar well diffusion assay and agar microdilution method (Minimum inhibitory concentration [MIC] and Minimum bactericidal concentration [MBC]). The best antibacterial activity, calculated as a zone of inhibition and MIC, MBC values. The Crude seed extracts of three-member Artocarpus showed antibacterial activity against the MRSA in the agar well diffusion assay (1.5-9 mm inhibition diameter). The MIC value of extract showed at 15.62 mg/mL and the MBC value of seed extract of A. heterophyllus at 62.5 mg/mL, A. champeden at 31.25 mg/mL, A. camansi at 250 mg/mL. The data have revealed that all seed extracts have the potential to be developed as antibacterial agents, particularly against MRSA strain. Further in vivo research and discovery of mode action are needed to shed light on their antibacterial effects. Research on anti-MRSA of seed extract of Artocarpus against MRSA has not been reported. Studies on the antibacterial activity against MRSA can provide new information about the benefits seed of members of Artocarpus as a source of natural antibacterial.
Antimicrobial Activity and Identification The Compounds of Methanol Extract from The Pleurotus Ostreatus Fruiting Body Prastiyanto, Muhammad Evy; darmwati, Sri; iswara, Arya; Setyaningtyas, Agnis; Trisnawati, Laily; Syafira, Anna
El-Hayah : Jurnal Biologi Vol 6, No 1 (2016): EL-HAYAH (VOL 6, NO 1,September 2016)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i1.4082

Abstract

Pleurotus ostreatus is an edible mushroom that also has potential as medicinal values. In this study, fruiting body of P. ostreatus was tested for its ability to inhibit the growth of fungi and bacteria. The fruiting body powder of P. ostreatus was extracted using methanol by maceration method. Analysis of this compound was done by using anisaldehid sulfuric acid, Dragendorff reagent, and FeCl3. Using the agar well diffusion technique, the extracts were tested for their antimicrobial activity against Staphylococcus aureus (Gram positive), Enterobacter aerogenes (Gram negative) and Candida albican (yeast). The spot results on TLC using crude extract of P. ostreatus is terpenoids. Zone of inhibition for the various extracts varied between 10.9 - 23.2 mm. Ten miligrams extract exhibit maximum antimicrobial activity against most of the tested pathogens 
Co-Authors Agmala, Aulia Bella Arya Iswara Astuti, Nita Ayu Dwi Azizah, Inas Hasna darmwati, Sri Fandhi Adi Wardoyo Haqi, Hafizha Dara Putri, Arifani Rahma Radipasari, Zhafira Dias Setyaningtyas, Agnis Sri Darmawati Syafira, Anna Trisnawati, Laily Wilson, Wildiani Yulianto, Bagus Dwi