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PRODUKSI GAS KARBON DIOKSIDA SELAMA PROSES BIOREMEDIASI LIMBAH HEAVY OIL DENGAN TEKNIK LANDFARMING Yani, Moh.; Setiadi, Yadi; Anas, Iswandi; Mas?ud, Zainal Alim; Charlena, Charlena
CHEMISTRY PROGRESS Vol 3, No 1 (2010)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.3.1.2010.66

Abstract

Bioremediation is defined as biological degradation process of organic or inorganic wastes in a controlledcondition to control and reduce their amount in the environment. In this study, the soil which had been polutedby heavy petroleum oil fractions, i.e. heavy oil waste (HOW), was used as sample. The bioremediationtechnique used in this study is an ex-situ technique. This study was conducted to determine the amount ofproduced CO2 gas during the HOW degaradation process. Treatment of heavy oil waste with biostimulation andbioaugmentation. Production of CO2 gas were placed in impiger and analysis with titrimetry method. The highestCO2 production mean was obtained from the bioaugmentation treatment with compost adding, i.e. 244,5 mg/m3.Generally, graphs CO2 gas production followed a similar sinusoidal pattern. From these results it can beinfered that the degradation process of HOW happened during the treatments in aerobic condition.
Isolation, Selection and Identification of Polyaromatic Hydrocarbons (PAHs) Degrading Bacteria from Heavy Oil Waste (HOW)-Contaminated Soil Yani, Mohamad; Charlena, Charlena; Mas?ud, Zainal Alim; Anas, Iswandi; Setiadi, Yadi; Syakti, Agung Dhamar
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.935 KB) | DOI: 10.4308/hjb.27.2.142

Abstract

The heavy oil waste (HOW) containing polyaromatic hydrocarbon (PAHs) is a persistent organic pollutants (POPs) that difficult to degrade. The new PAH degrading consortium was investigated from HOW contaminated soil in North Sumatera of Indonesia. The isolation, selection and identification of polyaromatic hydrocarbon degrading bacteria from soil contaminated by HOW was conducted to solve a bioremediation process. The isolation microbes from soil contaminated by HOW was performed using a minimum ONR7a media and followed on marine agar media for purification purposes. From the performed isolation results, 11 isolates were able to degrade PAHs compounds, such as phenanthrene, dibenzothiophene, or fluorene compounds. They grew at pH range of 4.8-8.2 and performed on emulsification activity in paraffin from 0.150-0.662. Three of them showed the best performance on HOW biodegradation capability and then successfully selected and identified as Salipiger sp., Bacillus altitudinis, and Ochrobactrum anthropi. using 16S rDNA. The HOW biodegradation as TPH-degradation were 38.66%, 59.60%, and 47.16%, respectively. Those isolated bacteria could potentially be as bioremediation agents to develop on bioremediation process for soils contaminated by HOW.