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Moderate-intensity interval exercise but not high-intensity interval exercise improves the spatial memory of ovariectomized rats Kaidah, Siti; Rauf, Saidah; Soesatyo, Marsetyawan HNE; Agustiningsih, Denny; Partadiredja, Ginus
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 3 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.401 KB) | DOI: 10.19106/JMedScie/005003201804

Abstract

Physical exercise exerts beneficial effects on the spatial learning and memory. High-intensity interval exercise (HIIE) has been proposed as a time-efficient physical exercise regimen. On the other hand, there were evidences that HIIE increased oxidative stress biomarkers and reduced antioxidant capacity, which resulted in oxidative damage. The present study aimed to investigate the effects of high-intensity interval exercise and moderate-intensity interval exercise on oxidative stress biomarkers and oxidative enzymes activity in the hippocampus and the spatial memory of ovariectomized rats. A total of 16 female Sprague Dawley rats aged 12 weeks were randomly assigned into 4 groups, i.e. the sham-operated (SO), ovariectomized without exercise (O), ovariectomized with high-intensity interval exercise (HIIE), and ovariectomized with moderate-intensity interval exercise (MIIE) groups. Rats of the exercise groups (HIIE & MIIE groups) performed 6 sessions of interval exercise per week for 6 weeks. The spatial memory of rats was measured using the Morris water maze procedure. The malondialdehyde (MDA) levels and activity of catalase (Cat) as well as glutathione peroxidase (GPx) in hippocampus were determined using spectrophotometry method. The spatial learning and memory retention of the moderate-intensity interval exercise group was significantly better than that of the high-intensity interval exercise group. The GPx activity of MIIE group was higher than any other groups. The SO group had the lowest hippocampal MDA level and highest Cat activity among groups. Moderate-intensity interval exercise reduces the ovariectomy induced-oxidative stress in the hippocampus and improves spatial learning and memory retention of ovariectomized rats.
Rosuvastatin Attenuated Elastic Fiber Degradation in Chronic Obstructive Pulmonary Disease Sprague-Dawley Rats Sabirin, Rahmaningsih Mara; -, Prasetyastuti; Agustiningsih, Denny
Jurnal Kedokteran Brawijaya Vol 30, No 3 (2019)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.817 KB) | DOI: 10.21776/ub.jkb.2019.030.03.2

Abstract

Chronic obstructive pulmonary disease (COPD) is an incurable disease which causes disability and death. The main pathogenesis of COPD is oxidative stress due to cigarette smoke which initiates various reactions and lead to lung elastic fibers destruction. Statins are known to have antioxidant effects and reduce mortality in COPD. We studied the effects of cigarette smoke exposure cessation and rosuvastatin on oxidative stress and the level of elastic fiber destruction in COPD model rats. Thirty 10-week old male Sprague-Dawley rats were divided into 2 groups: Control (n=6, did not received fumigation nor treatment) and Smoking (n=24, received fumigation for 70 days) groups. Afterwards, the smoking group was divided into 4 groups: Sham, R2, R5,R10, and received 0.9% NaCl, 2.5, 5 and 10 mg/kg/day of rosuvastatin, respectively. Examination of malondialdehyde (MDA) and desmosine serum were conducted to measure oxidative stress and elastic fiber degradation level, respectively. After smoke exposure, MDA and desmosine levels of COPD rats were found to be significantly higher (p=0.000 and 0.000) than controls. The MDA level in Sham, R2, R5 and R10 groups decreased significantly after therapy (p=0.000; 0.033; 0.015; 0.002). However, the post-treatment desmosine level was increase significantly in Sham and R2 groups (p=0.006 dan 0.012) and insignificantly (p=0.117 dan 0.278) in the R5 and R10 groups. It can be concluded that the cessation of exposure to cigarette smoke can reduce oxidative stress, but not elastic degradation process. The administration of rosuvastatin of 5 or 10 mg/kg/day attenuated elastic degradation process.
Gambaran Faktor Risiko Prolaps Organ Panggul Pasca Persalinan Vaginal di Daerah Istimewa Yogyakarta Pangastuti, Nuring; Sari, Dwi Cahyani Ratna; Santoso, Budi Iman; Agustiningsih, Denny; Emilia, Ova
Majalah Kedokteran Bandung Vol 50, No 2 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (677.733 KB) | DOI: 10.15395/mkb.v50n2.1321

Abstract

Prolaps organ panggul merupakan salah satu bentuk disfungsi dasar panggul pada perempuan. Disfungsi dasar panggul adalah keadaan terganggunya fungsi dasar panggul merupakan salah satu kondisi kesehatan yang banyak dikeluhkan perempuan. Lebih dari 46% perempuan dengan riwayat persalinan vaginal mengalami disfungsi dasar panggul,dan peningkatan jumlah paritas berkorelasi dengan peningkatan kejadian prolaps organ panggul. Tujuan penelitian ini adalah untuk mengetahui gambaran karakteristik faktor risiko prolaps organ panggul pasca persalinan vaginal di wilayah Daerah Istimewa Yogyakarta. Penelitian dilakukan pada Januari–Maret 2018. Hasil penelitian didapatkan 51 subjek penelitian dengan persalinan vaginal yang dapat dilakukan pemeriksaan POPQ pada 3 bulan pascapersalinan. Prolaps organ panggul didapatkan pada sebagian besar pasien pascasalin, yaitu sejumlah 46 orang, prolaps uteri 33 orang, sistokel 44 orang, serta rektokel pada 46) orang. Hanya 5 orang yang tidak memiliki gambaran prolaps organ panggul pada 3 bulan pascapersalinan.Dari penelitian ini dapat disimpulkan bahwa faktor risiko prolaps organ panggul pascapersalinan vaginal di Daerah Istimewa Yogyakarta terdiri atas faktor intrinsik (usia ibu, panjang genital hiatus maupun perineal body), dan ekstrinsik (paritas, indeks massa tubuh overweight dan obes, kenaikan berat badan selama hamil >15 kg, serta dilakukan episiotomi dan terjadi robekan perineum). Upaya pencegahan terutama edukasi, perbaikan gaya hidup, pengendalian indeks massa tubuh, pengaturan diet, olahraga penguatan otot dasar panggul, pembatasan jumlah kehamilan dan persalinan, serta pertolongan persalinan sesuai Asuhan persalinan normal. Kata kunci: Disfungsi dasar panggul, faktor risiko, persalinan vaginal, prolaps organ panggul Risk Factors for Pelvic Organ Prolapse in Women with History of Vaginal Delivery in YogyakartaPelvic organ prolapse is one form of pelvic floor dysfunctions in women that impairs the pelvic floor function and also one of the most frequently conditions complained by women. More than 46% women with history of vaginal delivery have experienced pelvic floor dysfunction where higher number of parity correllates to increased pelvic organ prolapse incident. The aim of this study was to understand the  characteristic risk factors of pelvic organ prolapse incident post vaginal delivery in Special Region of Yogyakarta. The study ws performed during the period of January to March 2018 on 51 subjects with history of vaginal delivery evaluated using POPQ in 3 months postpartum. Pelvic floor dysfunction was present in most postpartum patients, i,e, 46 people while 33 experienced uterine prolapse, 44 experienced cystocele, and 46 experienced rectocele. Only 5 subjects had no pelvic floor dysfunction in any form. Risk factors for pelvic organ prolapse in post vaginal delivery in the Special Region of Yogyakarta consist of intrinsic factors (maternal age, genital length of hiatus, and perineal body), and extrinsic factors (parity, overweight status and obese status based on body mass index, weight gain during pregnancy exceeding 15 kg,  episiotomy and the occurrence of perineal rupture). Prevention actions include  education followed by lifestyle improvement, control of body mass index, dietary regulation, exercise, restrictions on the number of pregnancies and childbirth, and safe delivery according to normal birth care standard.Key words: Pelvic floor dysfunction, pelvic organ prolapse, risk factors, vaginal delivery
The Influence of Dry Cupping toward Heart Rate Variability (HRV) in Male Obesity adolescence Syahruramdhani, Syahruramdhani; Agustiningsih, Denny; Sofro, Zaenal Muttaqien
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 16, No 2: July 2016
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v16i2.5137

Abstract

Cardiovascular disease defines as a disease related to heart and blood vessel. One of high risk in the disease of cardiovascular is its on people with obesity. Heart Rate Variability (HRV) indicator is an important sign to identify cardiovascular risk and provide early information related to change of heart autonomy controlling. This indicator was influenced by several factors such as cupping therapy. Study found that HRV increased after cupping therapy by wet cupping treatment in healthy people. The aims of this research is to investigate the influence of dry cupping toward increased HRV in male obesity adolescence. The study was experimental with pre-post test design by using consecutive sampling of 30 male adolescence aged 18-24 years old with BMI 25 kg/m2. They were divided into 2 groups, control and intervention group with cupping therapy. Data result used in SDDN and RMSSD level. Analysis Data were used paired and independent t test. The result showed SDNN and RMMSD level before intervention were 73,95 ms dan 67,11 ms. Whereas SDNN and RMMSD level after intervention were 69,66 ms dan 61,95 ms. In paired (p=0,52 and p=0,38) and independent t test (p=0,30 and p=0,56) showed that there were no significant difference between SDNN and RMSSD in intervention group and control group (p 0,05). The conclusion of this study is dry cupping had no effects toward increased HRV level in male obesity adolescence.