Nugroho, Trilaksana
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THE EFFECT OF GIVING BIT JUICE (Beta vulgaris L.) ON SPERMATOZOA MORPHOLOGY OF WISTAR RATS THAT EXPOSED MOSQUITO COIL SMOKE Septian, Kevin; Ariani, RR Mahayu Dewi; Hermawati, Donna; Nugroho, Trilaksana
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 9, No 2 (2020): DIPONEGORO MEDICAL JOURNAL ( Jurnal Kedokteran Diponegoro )
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.542 KB) | DOI: 10.14710/dmj.v9i2.27145

Abstract

Background : Spermatozoa have a limited amount of antioxidants that cause spermatozoa to be susceptible to oxidative stress by reactive oxygen species (ROS). Mosquito repellent contains the active ingredient Pyrethroid which is one example of external factors that cause DNA damage through oxidative stress. This results in the emergence of secondary metabolites that can act as free radicals, then these free radicals follow the circulation of blood throughout the body including the testes which will cause the process of spermatogenesis to be disrupted and will ultimately affect the quality of the spermatozoa produced. Objective :  To find out the effect of giving beetroot juice (Beta vulgaris) on the morphology of spermatozoa of male Wistar rats that exposed to mosquito coil smoke. Method : This study uses an experimental study, with the type of research design is post test only control group design. The population used in this study were male Wistar rats (Rattus novergicus). The research sample was selected by simple random sampling. Analysis of the data used in the study is an analysis with one way anova test. Results : The mean morphological morphology of normal spermatozoa are: Group K (-) = 96.40; Group K (+) = 88,40; P1 group = 90.80; P2 group = 94.40. One Way ANOVA test found significant differences between the five treatment groups. Post-Hoc Test found significant differences between K (-) with K (+), K (+) with P2, P1 with P2, P1 with K (-). While in the other groups, there was no significant difference in the morphological percentage of spermatozoa. Conclusion : The giving of beet juice can affect sperm morphology Wistar rats that exposed to mosquito coil smoke.Keywords : spermatozoa, bit juice, antioxidant, free radical
THE RELATION BETWEEN TYPES OF IRON CHELATORS AND FERITIN ON OSTEOCALCIN OF THALASSEMIA PATIENTS WITH REPEATED TRANSFUSIONS Amelia, Sesa; Nugroho, Trilaksana; Widyastiti, Nyoman Suci; Kholis, Fathur Nur
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 9, No 1 (2020): DIPONEGORO MEDICAL JOURNAL ( Jurnal Kedokteran Diponegoro )
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (427.715 KB) | DOI: 10.14710/dmj.v9i1.26565

Abstract

Introduction:  Thalassemia is an inherited disorder that decrease the rate of globin synthesis. Multiple blood transfusions in thalassemia patients leading to complications of hemosiderosis and hemochromatosis. Iron chelator aims to remove excess iron in the body. High ferritin levels interfere with 25-hydroxyvitamin-D production and negatively affect bone metabolism, measured with osteocalcin (N-Mid Oc). Study aims to determine the relation between type of iron chelator and ferritin levels to osteocalcin levels in thalassemia patients with history of repeated transfusions. Method: An observational analytic cross-sectional study. Research was conducted in May - August 2019 and carried out at Red Cross Semarang, Rembang Hospital, and Purwodadi Hospital. Study subjects were 40 people, then 6 people were excluded to 34 people. Gender are 14 male and 20 female. Subjects were thalassemia patient with repeated transfusions and undergoing iron chelation therapy. Subjects that met the criteria were tested for blood to measure ferritin levels and osteocalcin levels. Results: Average ferritin levels was 2842.85 ug/L and average osteocalcin levels was 15.05 ng/mL. There was significant relation between type of iron chelator on osteocalcin levels (p=0.046), but there was no significant relation between type of iron chelator on ferritin levels (p=0.434). There was significant relation between ferritin levels and osteocalcin levels on patient with Deferasirox therapy (p=0.022), but no significant relation on patient with Deferiprone therapy (p=0.432). Conclusion: There is significant relation between the type of iron chelator on osteocalcin levels and there is significant relation between ferritin levels and osteocalcin levels on patient with Deferasirox therapy.Keywords: thalassemia,iron chelator, deferasirox, deferiprone, ferritin, osteocalcin
FAKTOR-FAKTOR YANG BERHUBUNGAN DENGAN MIOPIA PADA ANAK SD DI DAERAH PERKOTAAN DAN DAERAH PINGGIRAN KOTA Wulansari, Dewi; Rahmi, Fifin Luthfia; Nugroho, Trilaksana
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 7, No 2 (2018): JURNAL KEDOKTERAN DIPONEGORO
Publisher : DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.738 KB)

Abstract

Latar Belakang Kelainan refraksi merupakan salah satu penyakit mata yang dapat menyebabkan kebutaan. Miopia merupakan salah satu bentuk kelainan refraksi. Data WHO tahun 2010 didapatkan prevalensi miopia di dunia sebesar 27% dan 2,8% untuk miopia tinggi. Faktor risiko terjadinya miopia adalah faktor keturunan dan faktor lingkungan. Diperlukan studi tentang faktor risiko dalam upaya pencegahan miopia.Tujuan Mengetahui dan menganalisis perbedaan prevalensi dan faktor-faktor risiko yang berhubungan dengan miopia miopia pada anak sekolah di perkotaan dan pinggiran kotaMetode Penelitian ini merupakan penelitian analitik observasional dengan pendekatan studi crossectional. Subjek penelitian adalah siswa SD kelas 4-6, dengan jumlah 59 di pinggiran kota dan 75 di perkotaan. Data dikumpulkan dengan cara wawancara menggunakan kuesioner. Uji statistik yang dilakukan adalah uji Chi-square.Hasil Prevalensi miopia di perkotaan 56%, sedangkan di pinggiran kota 28,8%. Didapatkan hasil yang signifikan antara jarak membaca buku (p=0,011), dan aktivitas di luar ruangan pada hari libur (p=0,002) dengan miopia di daerah perkotaan. Di daerah pinggiran kota didapatkan hasil yang tidak signifikan antara faktor-faktor yang diteliti dengan miopia. Didapatkan hasil yang signifikan antara letak geografis dengan miopia (p=0,002)Simpulan Prevalensi miopia lebih tinggi di perkotaan dibandingkan dengan piggiran kota. Faktor yang berhubungan dengan miopia di perkotaan adalah tingkat pendidikan orang tua, status ekonomi, jarak membaca buku, dan aktivitas di luar ruangan pada hari libur. Tidak terdapat hasil yang signifikan antara faktor-faktor dengan miopia di pinggiran kota. Terdapat hubungan antara letak geografis dengan miopia.
THE INFLUENCE OF BEETROOT (Beta vulgaris) ON SPERMATOZOA VITALITY OF WISTAR RATS EXPOSED TO MOSQUITO COILS Bharadaksa, Mohammad Arya Lingga; Ariani, Mahayu Dewi; Dewi, Puspita Kusuma; Nugroho, Trilaksana
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 9, No 3 (2020): DIPONEGORO MEDICAL JOURNAL ( Jurnal Kedokteran Diponegoro )
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.223 KB) | DOI: 10.14710/dmj.v9i3.27508

Abstract

Background: Spermatozoa vitality is one of the sperm analysis test. Application mosquito coil repellentgenerates free radical enhancement. Free radical contribute to external factor thatinfluence the quality of spermatozoa vitality. Beetroot(Beta vulgaris) contain a considerably high amount of antioxidant. It reduce free radical in the body and improve quality of spermatozoa vitality. Aim: To find out and analyze the effect of beetroot (Beta vulgaris) on the spermatozoa vitality of male wistar rats exposed to mosquito coils. Methods: This research uses experimental methods, with a post-test only control group design. The research sample was selected using a simple random sampling technique28 male wistar rats met the inclusion and exclusion criteria, then divided into 4 treatment groups K (-), K (+), P1 and P2.Data obtained after 56 days of treatment with 7 days of adaptation before treatment, the data will be tested for normality with Shapiro-Wilk then proceed one way ANOVA if the data distribution is normal. Results: Shapiro-Wilk test showed no significant differences between groups K (-), K (+), P1 and P2 (p> 0.05). One way ANOVA test on sperm vitality analysis showed a significant difference between groups. Post-Hoc test showed sperm vitality on K (+) there was significant difference compared to K (-) (p <0.001), K (+) no significant difference compared to P1 (p = 0.244) and K (+) there was significant difference compared to P2 (p = 0.001). Conclusion: Giving beetroot juice with the right dose has a significant effect on the spermatozoa vitality of male wistar rats exposed to the smoke of mosquito coils.