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PENGAMATAN KEBERADAAN BADAK SUMATERA (Dicerorhinus sumatrensis) DI KUTAI BARAT DAN MAHAKAM ULU KALIMANTAN TIMUR Hardiansyah, Gusti; Muslim, Ahmad; Tavita, Gusti Eva; Kurniawan, Yuyun; Kusuma, Arif Data
jurnal TENGKAWANG Vol 3, No 2 (2013): Jurnal Tengkawang
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jt.v3i2.21610

Abstract

Sumatran Rhino (Dicerorhinus sumatrensis) is the smallest rhinoceros and the most primitive types of the five species of rhinoceros that still alive. In the 1990s the status of the Sumatran rhino population in East Kalimantan never again recorded scientifically (Meijaard, 1996). In 2013 WWF Indonesia - Kutai Barat find signs of rhino are supported by the findings of the trail and a video camera recording automatically. The study aims to determine the status of the rhino habitat conditions and population. The experiment was conducted in Kutai Barat and Mahakam Ulu , East Kalimantan. In this study used a grid of 4x4 km ² ² divided into 2x2 cells with an area of 240 km² observations which represent 15 % of Zone 1 ( 1,600 km² ). The results showed high levels of habitat suitability with a population of 7-15 individuals.Key words : Habitat, population Sumatran Rhinoceros (Dicerorhinus sumatrensis)
KAJIAN GENETIKA UNTUK KONSERVASI BADAK SUMATERA (DICERORHINUS SUMATRENSIS GLOGER, 1841) Zein, Moch Syamsul Arifin; Fitriana, Yuli Sulistya; Kurniawan, Yuyun; Chaerani, Kurnia; Sirupang, Meriam
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3767

Abstract

ABSTRACTThe Sumatran rhinoceros is one of the most critically endangered species of large mammals due to habitat loss, fragmentation and illegal hunting so that the population of this species drastically decreased. At present, reproductive problems with a limited population are also a threat and require an appropriate solution. Therefore, data on molecular genetic information is very important as a basis for conservation management in maintaining long-term persistence of this species. Phylogenetic analysis based on sequences of CO1, 12SrRNA, and Cytochrome b gen from mitochondrial DNA genomes using neighbor-joining and genetic distance matrix calculations with the Kimura 2-parameter model (K2P) were implemented in pairwise distance calculations in the Mega (Molecular Evolutionary Genetics Analysis) program version 6.05. The study results show the genetic distance of Sumatran Rhinos from Sumatra and Kalimantan respectively 0.2 ± 0.00%, 0.8 ± 0.4%, and 0%. These results were reconfirmed that the Sumatran Rhino species in Sumatra and Kalimantan were taxonomy is no different. The study of genetic diversity based on D-loop of mitochondrial DNA contained 5 haplotypes, namely haplotypes 1 and 2 originating from the island of Sumatra and haplotypes 3, 4, and 5 originating from the island of Borneo. The genetic distance between individuals in this study ranged from 2.54 ± 1.4%, haplotype diversity (Hd) was 0.8 ± 0.172, nucleotide diversity (Pi) was 0.02269, Fu?s Fs value was 2.523, and Tajima?s test was 0.69497. The positive value (Fu?s Fs and Tajima?s test) indicated low genetic diversity and population expansion in the Sumatran rhino. In the study using 10 microsatellite loci, where the average number of allel/loci in Kalimantan (1.68) was higher than in Sumatra (1.22). Data from this study show that genetic variation between Sumatran rhinoceros from Sumatra and Kalimantan can be used as a basis for alternative that the populations of Sumatra and Borneo be considered as a single management unit.  Keywords: Sumateran  Rhinoceros, Mitochondrial DNA, Microsatellite
KAJIAN GENETIKA UNTUK KONSERVASI BADAK SUMATERA (DICERORHINUS SUMATRENSIS GLOGER, 1841) Zein, Moch Syamsul Arifin; Fitriana, Yuli Sulistya; Kurniawan, Yuyun; Chaerani, Kurnia; Sirupang, Meriam
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3767

Abstract

ABSTRACTThe Sumatran rhinoceros is one of the most critically endangered species of large mammals due to habitat loss, fragmentation and illegal hunting so that the population of this species drastically decreased. At present, reproductive problems with a limited population are also a threat and require an appropriate solution. Therefore, data on molecular genetic information is very important as a basis for conservation management in maintaining long-term persistence of this species. Phylogenetic analysis based on sequences of CO1, 12SrRNA, and Cytochrome b gen from mitochondrial DNA genomes using neighbor-joining and genetic distance matrix calculations with the Kimura 2-parameter model (K2P) were implemented in pairwise distance calculations in the Mega (Molecular Evolutionary Genetics Analysis) program version 6.05. The study results show the genetic distance of Sumatran Rhinos from Sumatra and Kalimantan respectively 0.2 ± 0.00%, 0.8 ± 0.4%, and 0%. These results were reconfirmed that the Sumatran Rhino species in Sumatra and Kalimantan were taxonomy is no different. The study of genetic diversity based on D-loop of mitochondrial DNA contained 5 haplotypes, namely haplotypes 1 and 2 originating from the island of Sumatra and haplotypes 3, 4, and 5 originating from the island of Borneo. The genetic distance between individuals in this study ranged from 2.54 ± 1.4%, haplotype diversity (Hd) was 0.8 ± 0.172, nucleotide diversity (Pi) was 0.02269, Fu?s Fs value was 2.523, and Tajima?s test was 0.69497. The positive value (Fu?s Fs and Tajima?s test) indicated low genetic diversity and population expansion in the Sumatran rhino. In the study using 10 microsatellite loci, where the average number of allel/loci in Kalimantan (1.68) was higher than in Sumatra (1.22). Data from this study show that genetic variation between Sumatran rhinoceros from Sumatra and Kalimantan can be used as a basis for alternative that the populations of Sumatra and Borneo be considered as a single management unit.  Keywords: Sumateran  Rhinoceros, Mitochondrial DNA, Microsatellite