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Kusumawati, Eko
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary

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Fitoremediasi Limbah Cair Laundry Menggunakan Melati Air (Echinodorus palaefolius) Dan Eceng Padi (Monochoria vaginalis) Apsari, Lela; Kusumawati, Eko; Susanto, Dwi
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 13 No 2 (2018): Bioprospek: Jurnal Ilmiah Biologi: Volume 13 Number 2 2018
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.571 KB) | DOI: 10.30872/bp.v13i2.424

Abstract

Limbah laundry merupakan limbah yang mengandung senyawa yang sulit terdegradasi dan dapat mencemari lingkungan. Salah satu cara untuk mengurangi pencemaran lingkungan yaitu fitoremediasi. Penelitian ini bertujuan untuk mengetahui pengaruh limbah laundry terhadap pertumbuhan melati air dan eceng padi serta untuk mengetahui kemampuan melati air dan eceng padi dalam menurunkan kadar deterjen. Pengambilan sampel limbah laundry di Jalan Pramuka, Samarinda. Uji pendahuluan dengan konsentrasi limbah laundry 0%, 25%, 50%, 75% dan 100% dengan melati air dan eceng padi sebanyak 200 gr, pengamatan selama 7 hari. Uji sesungguhnya dengan konsentrasi limbah laundry 25% dengan melati air dan eceng padi 0, 100, 200 dan 300 gr, diamati selama 14 hari. Pengukuran kadar deterjen, kualitas air serta pertumbuhan tanaman dilakukan sebelum dan sesudah pengamatan. Data dianalisis menggunakan uji One-way Anova dan Kruskal Wallis dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa tanaman melati air dan eceng padi dapat tumbuh dengan baik pada media limbah cair laundry. Hal ini ditunjukkan dengan semakin meningkatnya jumlah daun, panjang akar, berat kering dan laju pertumbuhan relatif melati air dan eceng padi.Tumbuhan melati air lebih efisien menyerap limbah deterjen. Hal ini dilihat dari nilai removal efficiency tertinggi pada tanaman melati air yaitu 76,68% sedangkan eceng padi sebesar 67,61%
Pengaruh Susu Fermentasi Lactobacillus Casei Terhadap Hematologi Mencit (Mus musculus) Yang Diinfeksi Salmonella enterica Serotype Typhi Nurlilayanti, Nurlilayanti; Kusumawati, Eko; Nugroho, Rudy Agung
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 14 No 1 (2019): Bioprospek: Jurnal Ilmiah Biologi: Volume 14 Number 1 2019
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.594 KB) | DOI: 10.30872/bp.v14i1.430

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh susu fermentasi Lactobacillus casei terhadap hematologi mencit yang diinfeksi Salmonella enterica serovar typhi (S. typhi). Tahapan penelitian ini yaitu pemeliharaan hewan uji, pembuatan media, perlakuan bakteri S. typhi pada mencit, pemeriksaan demam tifoid (uji widal), analisis hematologi mencit dan analisis data. Dalam penelitian ini, sekelompok mencit dibagi menjadi empat kelompok, yaitu K1 merupakan mencit kelompok kontrol negatif, K2 merupakan mencit kelompok kontrol positif, P1 mencit yang diberi perlakuan S. typhi inkubasi 6 jam (0,25 mL) dan susu fermentasi (0,25 mL) secara bersama-sama selama 7 hari dan P2 mencit diberi perlakuan S. typhi inkubasi 6 jam (0,25 mL) selama 7 hari dilanjutkan dengan pemberian susu fermentasi (0,25 mL) selama 5 hari. Hasil analisis uji statistik menyebutkan bahwa pemberian susu fermentasi pada mencit perlakuan P2, nilai hemoglobin (Hb) sebesar 4,20±0,20 dan hematokrit (Hct) sebesar 15,85±3,35, sedangkan pada P1 (mencit diinfeksi S. typhi dan pemberian susu fermentasi secara bersama-sama) tidak dipengaruhi, hal ini dikarenakan efek antagonis dari bakteri Lactobacillus terhadap bakteri Salmonella. Kesimpulan dari penelitan ini adalah bahwa mencit yang diberi perlakuan S. typhi dan susu fermentasi selama 7 hari tidak menunjukkan pengaruh terhadap hematologi mencit. Sedangkan pada mencit yang diberi perlakuan S. typhi selama 7 hari dilanjutkan dengan pemberian susu fermentasi selama 5 hari, berpengaruh terhadap hematologi mencit pada parameter Hb dan Hct.
IDENTIFIKASI SENYAWA METABOLIT SEKUNDER DAN UJI AKTIVITAS EKSTRAK ETANOL HERBA MENIRAN (Phyllanthus niruri L.) TERHADAP CANDIDA ALBICANS MENGGUNAKAN METODE DIFUSI CAKRAM Apriliana, Anita; Kusumawati, Eko; Selvitawati, Selvitawati
Jurnal Ilmiah Manuntung Vol 3 No 1 (2017): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung akademi farmasi samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.797 KB) | DOI: 10.51352/jim.v3i1.84

Abstract

The aim of this research is to know the ability of meniran ethanol extract (Phyllanthus niruri L.) in inhibiting the growth of Candida albicans. The research design is an experimental study. The extract used was herbal meniran extract made by maceration using 70% ethanol solvent, the content of ethanol extract of meniran tested against Candida albicans using disc diffusion method were 5%, 10%, 20%, 30% and 40%. Activity test conducted on Sabaraound Dextrose agar media that has been smeared with Candida albicans culture which has been standardized with Mc Farland standard. The pre-tested disc paper is first dipped into the extract with various concentrations. Incubated at 30 ° C for 24 hours and the inhibit zone formed was measured. The result showed that herbal ethanol extract of meniran resulted in drag zone diameter at Candida albicans ie 8,5 mm; 10.3 mm; 12.6 mm; 14.1 mm and 14.3 mm, for negative control 0 mm and positive control 20.1 mm. Data obtained from the results of the study were analyzed using one way anova method showed p - value <0,05, it was concluded that there was significant difference between negative control, 5%, 10%, 20%, 30%, 40% and positive control.
PENGARUH LAMA PEREBUSAN SIMPLISIA DAUN APAH (Albertisia papuana Becc.) YANG DIGUNAKAN SEBAGAI PENYEDAP MAKANAN OLEH MASYARAKAT KAB. TANA TIDUNG TERHADAP ANGKA CEMARAN MIKROBA Rosnah, Rosnah; Hendra, Medi; Kusumawati, Eko
Jurnal Ilmiah Manuntung Vol 2 No 1 (2016): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung akademi farmasi samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.159 KB) | DOI: 10.51352/jim.v2i1.41

Abstract

The purpose of this study is to determine the effect of long simplicia Apah’s leaves (Albertisia papuana Becc.) that’s used as a food’s flavoring by people of Kab. Tana Tidung against microbial contamination numbers. The design of research used a pattern completely randomized design (RAL), with a dilution series of samples 10-8, 10-9 and 10-10 and different boiling’s level (5, 10, 15, 20 and 25 minutes). The results obtained that the longer of boiling process is used, then the less the number of microbial contamination of colony growth. Average number of microbial contamination in samples in the boiling of 5 minutes is 92 x 1010 microbial colonies, in boiling 10 minutes is 87 x 1010 microbial colonies, in boiling 15 minutes is 56 x 1010 microbial colonies, in boiling 20 min is 44 x 1010 colonies of microbes and the boiling simplicia infuse for 25 minutes is 33 x 1010 colonies of microbes. Based of Analysis variance variety in each treatment showed that in boiling 20 minutes and 25 minutes showed real significant difference (p <0.05), but the boiling 5, 10 and 15 minutes there was no significant difference (P> 0.05). The results of the Anova, showed that in boiling treatment of simplicia Apah’s leaves (Albertisia papuana Becc.) significantly affects the growth of microbial colonies. Analysis of Least Significant Difference (LSD) at the level of 95%, the result that the effects of long boiling the simplicia Apah’s leaves (Albertisia papuana Becc.) which is used as a food flavoring by people of Kab. Tana Tidung, significant effect on the numbers of microbial contamination
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KECOMBRANG Etlingera elatior (Jack) R.M.Sm TERHADAP Salmonella typhi Kusumawati, Eko; Supriningrum, Risa; Rozadi, Reza
Jurnal Ilmiah Manuntung Vol 1 No 1 (2015): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung akademi farmasi samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.69 KB) | DOI: 10.51352/jim.v1i1.4

Abstract

Research Test Antibacterial Activity of Ethanol Extracts of Leaves kecombrang against Salmonella typhi aims to determine the antibacterial activity of ethanol extract of leaves kecombrang against Salmonella typhi. The extract used is kecombrang leaf extract prepared by maceration using ethanol 95%, extracts obtained test chemical classes of compounds to determine the content of the active compound. Antibacterial activity test conducted at five concentrations of the extract is 20%, 40%, 60%, 80%, and 100%. Inhibition zone measurement results are then analyzed using One Way ANOVA with SPSS 20 to determine whether there is a difference at each concentration. The results showed kecombrang leaf ethanol extract 20%, 40%, 60%, 80%, and 100% produce inhibition zone diameter 3.9 mm; 6.5 mm; 6.75 mm; 7:45 mm; and 9:28 mm, 0 mm for the negative control and positive control 32.61 mm. The test results show the class of secondary metabolites kecombrang leaf ethanol extract contains tannin, saponin, and flavonoids. Of statistical tests concluded there were significant differences of treatment results in inhibition of the respective concentrations of ethanol extracts of leaves kecombrang
IDENTIFIKASI BAKTERI AIR MINUM ISI ULANG DARI DEPOT YANG MENGGUNAKAN SUMBER AIR NON PDAM DI KOTA SAMARINDA Aulia, Nur Maulida; Sudrajat, Sudrajat; Kusumawati, Eko
Jurnal Ilmiah Manuntung Vol 3 No 2 (2017): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung akademi farmasi samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.82 KB)

Abstract

This aim of the present work is to know the type and the number of contamination in drinking water refill from depot using Non-PDAM water resource in Samarinda City. The sample were obtained from five subdistricts such as North Samarinda, Sambutan, Palaran, Loa Janan Ilir and Samarinda Ilir. Identification of bacteria using PCA (Plate Count Agar) media, BA (Blood Agar) and MCA (Mac Conkey Agar). Potentially pathogenic colonies tested for biochemical include SIM (Sulfid Indol Motile), SC (Simon Citrate), MR (Methyl red), VP (Voges Proskauer), TSIA (Triple Sugar Iron Agar), Nitrate, Urea, PAA (Phenyl Alanin Agar) and Glucose of. The results show that there is bacterial contamination in drinking water refill from depot using Non-PDAM water resource such as genus Staphylococcus, Klebsiella and Acinetobacter. The number of bacterial contamination were found at least in samples C1 and C2 from Sambutan, sample D2 from Palaran and the most contamination number was found in sample A1 from North Samarinda.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KEREHAU (Callicarpa longifolia Lam) TERHADAP Escherichia coli dan Staphylococcus aureus Kusumawati, Eko; Apriliana, Anita; Khatimah, Khusnul
Jurnal Ilmiah Manuntung Vol 2 No 2 (2016): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung akademi farmasi samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.314 KB) | DOI: 10.51352/jim.v2i2.63

Abstract

Kerehau (Callicarpa longifolia Lam) is a plant that has traditionally efficacious as a cure colds and diarrhea. Part used as medicine are the leaves. This study aims to determine the antibacterial activity of ethanol extract of leaves kerehau on Escherichia coli and Staphylococcus aureus. Kerehau leaf extract prepared by maceration method using ethanol 70%, with a test concentration of 10%, 25%, 50%, and 100% and chloramphenicol 0.1% (w / v) as a positive control and dimethyl sulfoxide 1% (v / v) as a negative control. The results of antibacterial activity test with extract concentrations of 25%, 50% and 100% in Escherichia coli is 9.45 mm, 12.21 mm and 17.38 mm, for the positive control is 21.85 mm. The results of antibacterial activity test with extract concentrations of 10%, 25%, 50% and 100% in Staphylococcus aureus was 7.3 mm, 9.85 mm, 13.65 mm and 20.58 mm, for the positive control was 24.05 mm. The antibacterial activity was analyzed by One Way ANOVA showed significant differences between treatments
UJI CEMARAN BAKTERI COLIFORM PADA MINUMAN AIR TEBU Sukawaty, Yullia; Kamil, Muhammad; Kusumawati, Eko
Jurnal Ilmiah Manuntung Vol 2 No 2 (2016): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung akademi farmasi samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.962 KB) | DOI: 10.51352/jim.v2i2.73

Abstract

Sugarcane juice beverage is a beverage that is found along the village Sempaja and Pelita roads. Contamination in processed beverages derived from sugarcane juice and equipment used allow for contamination by coliform bacteria. The purpose of this study to investigate the contamination of coliform bacteria in water beverage sugar circulating in the village Sempaja and Pelita. Tests using the Most Probable Number (MPN) includes test estimators using lactose broth as the presumptive medium and Brillian Green Lactose Bile Broth as the confirmad medium. The results showed that eight samples tested positive for coliform contaminated with a high of 2800×101 MPN / mL (sample H) and the lowest value 170×101 MPN/mL (sample D). The results obtained show that the sugarcane juice drinks that supply does not meet the standards of Rule Head POM HK.00.06.1.52.4011 No. 2009 with standard quality requirements of 2×101colonies/mL.
Co-Authors Anita Apriliana, Anita Apsari, Lela Aulia, Nur Maulida Dwi Susanto Jamhari Jamhari Khatimah, Khusnul Medi Hendra, Medi Nurlilayanti, Nurlilayanti Risa Supriningrum Rosnah, Rosnah Rozadi, Reza Rudy Agung Nugroho Selvitawati, Selvitawati Sudrajat Sudrajat Sukawaty, Yullia