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HORMONE PROFILE OF DISORDER OF SEX DEVELOPMENT (DSD) PATIENTS IN PALEMBANG Maritska, Ziske; Prananjaya, Bintang Arroyantri; Parisa, Nita; Quardetta, Rovania Yantinez
Biomedical Journal of Indonesia Vol 5, No 2 (2019)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v5i2.7987


Apart from being based on clinical findings, the determination of the diagnosis of Disorders of sex development (DSD) is also supported by a variety of supporting examinations such as chromosome analysis, radiology, and laboratory examinations, one of which is the examination of hormone levels. Examination of hormone levels can provide a picture of gonadal and adrenal function, which is useful both for screening and for determining the diagnosis of DSD. The study that aims to identify the hormone profile of DSD patients in Indonesia is still very minimal, and this study is the first study to review the profile of DSD hormones in RSUP Dr. Mohammad Hoesin Palembang. This study is a descriptive study using secondary data from the medical records of DSD patients who underwent hormonal examinations during the 2013-2017 period. Of the total 173 DSD patients who came for treatment at the Dr. Mohammad Hoesin Palembang during the last five years, only 22 (12.72%) patients underwent hormone testing. There were 13 hormone profiles that were examined in DSD patients at RSUP Dr. Mohammad Hoesin Palembang with the hormone testosterone (54.54%) as the most frequently examined hormone. Hormone profile examination as a screening aid and diagnosis of DSD has not become one of the most common supporting examinations carried out at Dr. RSUP. Mohammad Hoesin Palembang.
Biomedical Journal of Indonesia Vol 5, No 3 (2019)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v5i3.9872


Hyperthyroidism is a condition where there is an increase in thyroid hormone synthesis and secretion so that the level of thyroid hormone in the blood increases. In 2013, data showed 0.4% of Indonesia's population experienced hyperthyroidism. Ciplukan fruit (Physalis peruviana) is an Indonesian herb that is thought to have an effect on thyroid hormone. This research is an in vivo experimental study. The research subjects were 30 Wistar strain white rats divided into 5 groups: negative control (distilled water), positive control (PTU 100 mg / kgBW), groups 3, 4, and 5 Ciplukan fruit extracts at a dose of 250 mg/kgBW, 500 mg/kgBW, and 1000 mg/kgBW. The results of the examination showed a decrease in T4 levels in the treatment with positive control and administration of ciplukan fruit extracts. There was a difference in the reduction in T4 levels for the group giving ciplukan extract doses of 1, 2, and 3 (p <0.05). It can be concluded that Ciplukan fruit extract with a dose of 250 mg/kgBW, 500 mg/kgBW, and 1000 mg/kgBW has potential as an anti-hyperthyroidism.
Identifikasi Polimorfisme Gen mTOR rs 2536 dan rs 2295080 serta Ekspresi ER, PR, HER-2, Ki-67 pada Penderita Kanker Payudara Haq, Anisah Nida'ul; Triwani, Triwani; Parisa, Nita
Sriwijaya Journal of Medicine Vol. 2 No. 3 (2019): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1046.633 KB)


Kanker payudara adalah suatu penyakit keganasan yang berasal dari transformasi neoplastik sel-sel epitelial, sebagai akibat akumulasi perubahan genetik, epigenetik dan lingkungan yang dibuktikan dengan pemeriksaan histopatologi. Kanker payudara menempati urutan pertama sebagai jenis kanker yang paling umum diderita oleh perempuan di dunia. Salah satu predisposisi genetik yang berperan pada terjadinya kanker payudara adalah polimorfisme gen mTOR yang terjadi pada 3’-UTR dan regio promoter. Polimorfisme gen mTOR akan menyebabkan aktivasi proses transkripsi gen mTOR secara terus-menerus sehingga terjadi proliferasi dan diferensiasi sel yang abnormal. Penelitian ini bertujuan untuk mengidentifikasi polimorfisme gen mTOR rs 2536 dan rs 2295080 serta ekspresi ER, PR, HER-2 dan Ki-67 pada penderita kanker payudara di Rumah Sakit Mohammad Hoesin Palembang. Penelitian ini adalah penelitian laboratorium berjenis deskriptif observasional dengan teknik PCR-RFLP menggunakan enzim EcoRI dan FokI terhadap 33 penderita kanker payudara berdasarkan metode purposive sampling. Polimorfisme gen mTOR rs 2536 paling banyak terdapat pada genotip mutan homozigot (CC) yaitu terdapat pada 27 orang (81,8%) dengan frekuensi alel C sebesar 89,4% sementara alel T hanya 10,6%. Genotip yang paling banyak ditemukan pada polimorfisme gen mTOR rs 2295080 adalah wild type (TT) yang terdapat pada 19 orang (57,6%) dengan frekuensi alel T sebesar 63,6% sementara alel G sebanyak 36,4%. Data dari bagian Patologi Anatomi menunjukkan adanya kecendrungan meningkatnya ekspresi Ki-67, ER, PR dan HER-2 pada polimorfisme gen mTOR rs 2536 dan rs 2295080. Polimorfisme gen mTOR rs 2536 (CC) pada kanker payudara lebih banyak dibanding polimorfisme gen mTOR rs 2295080 (GG).
Neuronal Cell Death Induces Depressive Disorder in Rats Depression-Like Behaviors Caused by Chronic Stress Hidayat, Rachmat; Saleh, Mgs Irsan; Parisa, Nita
Scientia Psychiatrica Vol. 1 No. 2 (2020): Scientia Psychiatrica
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/scipsy.v1i2.8


Abstract Introduction Depression is believed to be a disorder in which an increase in serotonin activity in the brain. This has implications for the development of various antidepressant drugs that work to increase serotonin levels, by inhibiting serotonin reuptake. However, management with antidepressants is still believed to be not optimal, there are still various problems that have not been able to be solved only by increasing serotonin levels in the brain. Therefore, it is necessary to do further exploration to find out other possible pathophysiology of depressive disorders. This study intended to explore the role of apoptosis of neuronal cells in the prefrontal cortex to answer the hypothesis that depression was not only caused by increased serotonin levels but also there was a role of dead neuronal cells in the prefrontal cortex which will trigger the body's homeostatic efforts to compensate by increasing serotonin levels. Methods A total of 30 male Wistar rats (200 ± 20 g) were obtained from Eureka Research Laboratory (Palembang, Indonesia). Experimental animals were placed in cages under controlled conditions (12 hours of light / dark cycles with temperatures of 22 ± 1˚C and humidity of 40-60%), fed and drank ad libitum. Experimental animals with depression model were induced using Chronic Mild Stress (CMS). CMS procedures were performed with mild stressors such as repeated cold stress (4 ° C), space reduction in the homecage, changed cages and social interaction with other animals of the CMS group. To assess wether animal were being depression or not, the animal were tested using Forced Swimming Test (FST). After induction, rats were randomly divided into two groups which each contained 15 animals: the normal control group (not induced CMS) and the CMS group (negative control). Furthermore, the animal model was performed perfusion to maintain organ when evacuation was done, cell damage did not occur. To evaluate cell organ, immunohistochemistry examination and ELISA examination was performed. All data are presented as mean ± standard deviation and all statistical analyzes are performed with the SPSS 25 (IBM) program. Result This research showed that CMS animal model has a greater duration of immobility than the normal group and serotonin level in CMS animal models decreased almost threefold compared to the normal group. In addition, there were increased expression of caspase-3 indicates that more neuronal cells suffered from apoptosis. So, in this research, it was clearly stated that in depressive disorder, there were elevation of neuronal cell apoptosis in the prefrontal cortex. Conclusion Neuronal cell apoptosis in the prefrontal cortex plays a role in the pathophysiology of depression through activation of negative feedback on serotonin production.
Edukasi pencegahan sindrom kelelahan (burnout) pada karyawan Pertamina MOR II Palembang larasati, veny; Arroyantri P, Bintang; Maritska, Ziske; Parisa, Nita; Syauki Ikhsan, Diyaz
Jurnal Pengabdian Masyarakat: Humanity and Medicine Vol 1 No 3 (2020): Jurnal Pengabdian Masyarakat: Humanity and Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/Hummed.V1I3.13


Education for Preventing Burnout Syndrome to Pertamina MOR II Palembang workers. Burnout is a syndrome that occurs due to prolonged interpersonal stress due to work. Burnout can also be described as psychological fatigue caused by work. This can occur due to the high demands of the company to provide optimal work results that the employees cannot tolerate. There are three things that can trigger this syndrome, namely excessive workload, neglect of work results and feelings of underappreciation at work. Based on a survey conducted in America, the main reason of stress was due to excessive workload, 1 in 5 employees experienced burnout syndrome which is around 46%, and in Indonesia, the survey results showed that around 64% of employees experienced increased fatigue due to work, and around 20% of employees believed that psychological fatigue is the cause of the absence of employees due to illness in the company. This community service is carried out at the request of the PERTAMINA Palembang Company. Burnout syndrome is a serious problem for employees and employers. If not addressed properly, it will lead to serious psychological problems for employees such as decreased productivity, withdrawal from work, depression and even anxiety, which in turn can have a negative impact on the company. For this reason, it is necessary to have monitoring and evaluation related to the psychological condition of employees on a regular basis.