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Sterilization Effectiveness Of Rubber Leaf Explant (Hevea brasiliensis) In In-Vitro Culture Lukmana, Mila; Rahmawati, Linda
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 13 No 1 (2018): Bioprospek: Jurnal Ilmiah Biologi Volume 13 Number 1 2018
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.189 KB) | DOI: 10.30872/bp.v13i1.201

Abstract

Rubber (Hevea brassiliensis), are the commodities that have the potential to be developed. Rubber cultivation is currently only rely on rootstocks obtained from seeds in the nursery, so availability is limited by seasons and seed viability. Tissue culture techniques to address challenges to obtain seeds in large quantities in a short time. Tissue culture are often faced with the problem of contamination, so that the required ingredients for the success of the sterilizing effective planting rubber leaf explants in vitro. Variables observed among other things, the speed of contamination of explants, number of explants of living, the number of types of contaminants, the number of sources of contaminants as well as the number of explants browning and not because browning. Sterilization treatment in this study, namely P1 without sterilization in LAFC, P2 with 70% alcohol, P3 combination treatment NaOCl 20% and 70% alcohol, P4 uses a combination of HgCl2 a 0.01% and NaOCl 10% and P5 using 70% alcohol and H2O2 17.6 % (v/v). The results showed that the treatment P1 bacterial contamination of 60% and mushrooms 85%, P2 bacterial contamination by 90% and mushrooms 10%, P3 bacterial contamination 92.5% and mildew 17.5%, P4 bacterial contamination 72.5% and fungi 10% and the treatment P5 with 65% of bacterial contamination without contamination of mushrooms. Best results are obtained by using the P5 treatment Alcohol 70% for 1 minute and H2O2 17.6% (v / v) for 20 minutes can remove contaminants fungi and suppress bacterial contamination percentage of 65% to 30 days after planting.
PEMBERDAYAAN MASYARAKAT MEMBUAT BIOCHAR DAN KOMPOS DARI BIOMASSA TANAMAN PADI Abdillah, Muhammad Helmy; Majid, Zuliyan Agus Nur Muchlis; Iswahyudi, Herry; Lukmana, Mila; Rahmawati, Linda; Widiyastuti, Dewi Amelia
JMM (Jurnal Masyarakat Mandiri) Vol 5, No 4 (2021): Agustus
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (31.837 KB) | DOI: 10.31764/jmm.v5i4.4812

Abstract

Abstrak: Biomassa tanaman Padi manjadi masalah dalam sanitasi lahan rawa pasca panen, meskipun biomassa ini dapat digunakan untuk pembanah tanah dengan pemanfaatannya belum optimal. Kearifan lokal masyarakat suku Banjar yang sudah mulai ditinggalkan diduga menjadi penyebab masalah tersebut. Kearifan lokal ini mendorong penggunaan biomassa Padi secara optimal untuk membenahi tanah sub-optimal pada tanah-tanah di lahan rawa. Kegiatan ini bertujuan untuk meningkatkan pengetahuan tentang pembuatan biomassa Padi menjadi pembenah tanah dengan konsep kearifan lokal tajak-puntal-balik-hambur yang dikemas dalam teknologi sederhana berbentuk biochar dan kompos berbahan biomassa Padi. Metode yang digunakan pada kegiatan ini berupa pelatihan pada 36 orang masyarakat kecamatan Alalak yang pada akhir pelatihan dievaluasi pengetahuannya menggunakan formulir berisi pilihan ganda dengan pertanyaan sederhana terkait minat, kebermanfaatan dan materi pelatihan ini. Hasil menunjukkan bahwa 89% terbentuknya pemahaman baru tentang fungsi, cara pembuatan dan manfaat biochar dan kompos sehingga konsep kearifan lokal masyarakat Banjar tetap dilakukan dalam model yang baru. Pembuatan biochar dan kompos dapat mengurangi masalah sanitasi lahan pada lahan budidaya Padi dan menurunkan masalah tanah di lahan sub-optimal.Abstract: Paddy's biomass is a problem in post-harvest swampland sanitation. Even though this biomass can be used for various types of soil amendment, its utilization is not yet optimal. The local wisdom of the Banjarase that has begun to be abandoned is suspected to be the cause of the problem. This local wisdom encourages the optimal use of Paddy biomass to repair on swampy soils. This activity aims to increase knowledge on the manufacture to be soil amendment with the local wisdom concept as name tajak-puntal-balik-hambur which is packaged in a simple technology in the form of biochar and compost made from rice biomass. The method used in this activity was in the form of training for the community of Alalak sub-district as many as 36 people whose knowledge was evaluated using a form containing multiple choices with simple questions related to interest, usefulness, and training of this materials. The results of the activity show that 89% new understandings emerge on the function, manufacture and benefits of biochar and compost from rice biomass so that the concept of local wisdom Banjarase is still carried out in a new model. Making biochar and compost can reduce land sanitation problems in Paddy cultivation land and reduce soil problems in sub-optimal land