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PENGARUH SINAR ULTRAVIOLET TERHADAP PERTUMBUHAN BAKTERI Enterotoxigenic E.coli (ETEC) PENYEBAB PENYAKIT DIARE Risky Vidika Apriyanthi; I Gst. AA Ratnawati; Retno Kawuri
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 6 No. 1 (2021): Bioma, Januari - Juni 2021
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v6i1.12157

Abstract

. Telah dilakukan penelitian mengenai pengaruh sinar ultraviolet terhadap pertumbuhan bakteri Enterotoxigenic E.coli  (ETEC). Penelitian dilakukan untuk mengetahui jarak, lama penyinaran, persentase kematian dan pengaruh sinar ultraviolet yang dapat mematikan bakteri ETEC. Metode penanaman dan penghitungan total koloni bakteri yang digunakan adalah plating method. Selanjutnya dilakukan pewarnaan gram untuk melihat bentuk sel bakteri uji.Hasil penelitian menunjukkan, persentase kematian bakteri ETEC tertinggi yaitu pada jarak 15 cm dengan lama penyinaran 15 menit.dengan intensitas 40 lux, sebesar 91,36%. Sedangkan pada jarak 30 cm dengan lama penyinaran 5 menit dan intensitas 19 lux, hanya berhasil membunuh bakteri sebesar 19,52%. Sel bakteri ETEC yang telah diberi penyinaran, bentuk sel bakteri ETEC mengalami perubahan menjadi elips, ada yang mengalami lysis dan susunan rantai terputus. Kata Kunci : Ultraviolet, jarak, lama penyinaran, Enterotoxigenic E.coli  (ETEC).  
Analisis Pigmen Alami Daun Mangga (Mangifera Indicalinn) Sebagai Pewarna Batik Dengan UV-VIS Ni Komang Tribuana Cipta Putri; I Gst Agung Ayu Ratnawati; Wayan Gede Suharta
Kappa Journal Vol 3, No 2 (2019): Desember
Publisher : Universitas Hamzanwadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29408/kpj.v3i2.1627

Abstract

The development of the batik industry has caused batik consumption to increase. Increased clothing and lifestyle needs trigger the need for practical (synthetic) dyes to have an impact on the waste produced. To reduce negative effects, the use of natural dyes needs to be increased. One of the plants that produce natural dyes is mango (Mangifera Indica Linn). Mango plants (Mangifera Indica Linn) including the family Anacardiaceae. The leaves contain a lot of chlorophyll which can produce color pigments for textiles. To find out the potential, an UV-VIS analysis was carried out, namely absorbance and calculation of the amount of chlorophyll contained in the leaves. The method used is the maceration process with technical ethanol solvents, with variations in concentration (70% and 96%) and variations in the immersion time (3 hours, 5 hours, 10 hours, 20 hours). From the results of UV-VIS spectrophotometry analysis showed the maximum absorption or absorbance at a concentration of 96% for 5 hours and the calculation of chlorophyll by the Lichtenthaler Welburn (1983) method showed that the sweet arum manggu leaves used for research contained more chlorophyll b than chlorophyll a, gives a greenish-yellow pigment with wavelengths of 540 nm and 640 nm.
PREPARATION, CHARACTERIZATION AND USED OF POLYSULFONE MEMBRANES FOR THE TREATMENT OF WATER SOLUTIONS CONTAINING HUMIC ACIDS Ni Nyoman Rupiasih; Made Sumadiyasa; A.A. Ratnawati; Hery Suyanto
Jurnal Sains Materi Indonesia VOL 13, NO 1: OKTOBER 2011
Publisher : Center for Science & Technology of Advanced Materials - National Nuclear Energy Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.879 KB) | DOI: 10.17146/jusami.2011.13.1.5400

Abstract

PREPARATION, CHARACTERIZATION AND USED OF POLYSULFONE MEMBRANES FOR THE TREATMENT OF WATER SOLUTIONS CONTAINING HUMIC ACIDS. Polysulfone (PSf) membranes were cast by phase inversion method using PSf as matrix, N,N-Dimethylacetamide (DMAc) as solvent and distilled water as coagulant. The microporous membrane has studied using Scanning Electron Microscope (SEM) and filtration techniques. The filtration experiments were performed on a flat sheet membrane using pure water and humic acids (HA) solution as feeds. The results show, the Pure Water Flux (PWF) and Product Flux (PF) values decrease with increasing thickness of the membranes. They are consistent with the SEM images that thicker membranes have smaller pore size and less in numbers. The Rejection coefficient (R) values showthat the membranes are able to filter HA in the form of solution, with the performance dependent on the characteristics: thickness and microporous structure. They were increased with increase in the thickness of the membranes and with increase in the wavelength from 250-665 nm. It suggests that, the PSf membranes can be used to minimize the amount of Humic Acids from water process to improve the quality of treated to an acceptable quality level.
UJI KANDUNGAN UNSUR RADIOAKTIF DAN BAKTERI PENCEMAR ESCHERICHIA COLI PADA LIMBAH INDUSTRI DI DAERAH HILIR SUNGAI BADUNG, DESA PEMOGAN Ni Made Indah Suandewi; I Gusti A.A. Ratnawati; Ida Bagus Gede Darmayasa
BULETIN FISIKA Vol 16 No 1 (2015): BULETIN FISIKA
Publisher : Departement of Physics Faculty of Mathematics and Natural Sciences, and Institute of Research and Community Services Udayana University, Kampus Bukit Jimbaran Badung Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The radioactive element content and E.coli bacterial contaminants has been done testing in river water downstream of Badung River, Village Pemogan. The testing of elements content in the samples using AAS instrument (Atomic Absorption Sphectrometer) Analytical Laboratory Udayana University. The elements contained in the river water downstream of Badung River, Village Pemogan including Co, Cu, Pb, and Cd. The pollution in Badung river water to the elements thought due to the disposal of waste production by the textile dye industry was around the River Badung, Pemogan village. The test pollutant content of E.coli bacteria using the MPN (Most Probable Number). The results of the test bacteria by MPN method showed the presence of E.coli bacteria contaminants in Badung river water with amounts ranging from 0 to >11,000 samples MPN/100ml. According to PP 82 in 2001, the content of E.coli bacteria in the water of the river is allowed a maximum in the range of 2000 MPN/100ml water. This suggests that the content of E.coli bacteria in river water downstream of Badung River, Village Pemogan has exceeded a predetermined threshold.Keywords : Radioactive Elements, E.coli, AAS (Atomic Absorption Sphectrometer), MPN (Most Probable Number)
PENGARUH GRID RATI TERHADAP TINGKAT KEHITAMAN FILM DENGAN MENGGUNAKAN SINAR-X Yetti Herlina; I Gusti Agung Ayu Ratnawati; I Wayan Balik Sudarsana
BULETIN FISIKA Vol 16 No 1 (2015): BULETIN FISIKA
Publisher : Departement of Physics Faculty of Mathematics and Natural Sciences, and Institute of Research and Community Services Udayana University, Kampus Bukit Jimbaran Badung Bali

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Abstract

Radiography quality is radiography ability to provide clear information about the object being examined. One of the important things that punch in a radiography with respect to the quality of the radiography contrast. Radiography contrast is obtained by measuring the density with a densitometer. A good radiography can be viewed from various aspect, one of which is contrast, lack of radiation scattering that occurs is essential to obtain optimal contras, it can be obtained in several ways such as irradiation (collimation to taste) and the used of technict kV. Movie density level depends on the density of the material transmitted by, the density on the movie influenced in grid ratio. Density increases with increasing grid ratio. The larger the grid ratio used the greater the darknes of the movies. Measurement with uji t statistic paired where the hypothesis if the Pvalue (sig) ‹ ? (5%) then H0 is the rejected and if the Pvalue (sig) › ? (5%) then H0 is accepted and if the value thitung › ttabel then H0 is rejected and if the thitung ‹ ttabel then H0 ­is accepted so it can be inferrent grid ratio of the obtained significant results, so the larger the grid ratio comparison is used then the grid the greater the darkness of the movie.
Uji Kandungan Unsur Logam Berat Fe, Pb, Cu dan Cr Pada Air Tukad Badung, Desa Dauh Puri Kauh dengan Metode Atomic Absorption Spectrophotometry (AAS) Theresia Avila Mogi; I Gusti Agung Ayu Ratnawati; Putu Suardana
BULETIN FISIKA Vol 24 No 2 (2023): BULETIN FISIKA August Edition
Publisher : Departement of Physics Faculty of Mathematics and Natural Sciences, and Institute of Research and Community Services Udayana University, Kampus Bukit Jimbaran Badung Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/BF.2023.V24.i02.p09

Abstract

Research has been carried out to test the content of heavy metal elements in Tukad Badung water, Dauh Puri Kauh Village using the Atomic Absorption Spectrophotometry (AAS) method. This study aims to determine the levels of heavy metals Fe, Pb, Cu, and Cr in Badung Tukad. The results of the analysis showed that Tukad Badung water contained heavy metals Fe, Pb, Cu and Cr of 0.4616 ± 0.2378 mg/L, 1.8068 ± 0.7051 mg/L, 2.3035 ± 2.883 mg/L, respectively and 1.9215 ± 0.0766 mg/L.
Pengaruh Sinar Ultraviolet Terhadap Jamur Aspergillus Niger I Gusti Agung Ayu Ratnawati; I Ketut Putra; Ida Bagus Made Suryatika; Gusti Ngurah Sutapa; Ni Luh Putu Trisnawati
Kappa Journal Vol 7, No 1 (2023): April
Publisher : Universitas Hamzanwadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29408/kpj.v7i1.12252

Abstract

Along with the development of science and technology, some researchers try to apply knowledge to several biological systems to obtain benefits that can be used. One of them is the use of ultraviolet light to kill the fungus Aspergillus niger. In addition, ultraviolet rays are also used in various fields of the drinking water industry, food, and also for sterilizing operating rooms in hospitals. A method used in this problem is the cork borer method. Data were obtained by measuring the diameter of the inhibition zone of the Aspergillus niger fungus. The results of this study were obtained at the intensity of 12.30 lux and at a distance of 15cm, within 15 minutes of irradiation time, the maximum death of the fungus Aspergillus niger was 40.74%.
Analisis Diagnostic Reference Level (DRL) Pasien Pada Pemeriksaan Abdomen Kontras dan Nonkontras Dengan Pesawat CT Scan Di RSUD Bali Mandara Venesia Sesilia Br Ginting; Gusti Ngurah Sutapa; I Gusti Agung Ayu Ratnawati; I Komang Widiana
Kappa Journal Vol 7, No 1 (2023): April
Publisher : Universitas Hamzanwadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29408/kpj.v7i1.18985

Abstract

In this study, data were obtained from 35 patients aged 27-76 years. The current used varies from 46-185 mAs and a constant tube voltage of 130 kV, with different clinical symptoms. The patient's DRL value on contrast and non-contrast abdominal examination was determined by calculating the third quartile value. On non-contrast abdominal examination, the DRL CTDIvol value was 10.62 mGy and the DLP DRL was 607.76 mGy.cm and on the non-contrast abdominal examination, the DRL CTDIvol value was 32.73 mGy and the DRL DLP value was 1825.10 mGy.cm. These results indicate that the DRL CTDI vol and DRL DLP values on contrast abdominal examination at Bali Mandara Hospital were still below the actor's limits set by BAPETEN, while on abdominal examination the DRL CTDI vol and DRL DLP values exceeded the limits set by BAPETEN. This is caused by exposure which is done three times. Therefore, it is necessary to restore both the Patient Operational Standards (SOP) and the exposure factor on an abdominal contrast CT scan at Bali Mandara Hospital.