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Hubungan antara Ekspresi CD-10 dan Grading Histopatologi Karsinoma Payudara Invasif Fitri Dewi Ismida; Soekimin; Delyuzar
Majalah Patologi Indonesia Vol 28 No 2 (2019): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Latar belakangKarsinoma payudara invasif merupakan suatu keganasan epitelial payudara yang ditandai oleh invasi sel-selepitel ke dalam stroma. Secara global, insidensi karsinoma payudara invasif menduduki peringkat tertinggi danterus meningkat dari tahun ke tahun. Pada jaringan payudara normal, ekspresi CD-10 terlokalisir pada sel-selmioepitel dan tidak teridentifikasi pada stroma. Demikian juga, pada ductal carcinoma in situ (DCIS), CD-10tidak terekspresi pada stroma. Namun pada karsinoma payudara invasif CD-10 akan terekspresi pada stroma.CD-10 merupakan suatu cell-surface zinc-dependent metalloproteinase yang sering direkomendasikan sebagaimarker leukemia limfoblastik. Beberapa penelitian telah dilakukan untuk menilai hubungan antara ekspresi CD10 dan grading histopatologi karsinoma payudara invasif, namun sampai kini hasilnya masih kontroversi.Tujuan penelitian ini untuk menganalisis hubungan antara ekspresi imunohistokimia CD-10 dan gradinghistopatologi karsinoma payudara invasif.MetodePenelitian ini merupakan penelitian deskriptif analitik dengan pendekatan cross sectional. Sampel penelitianadalah blok parafin, berasal dari jaringan payudara yang didiagnosis secara histopatologi sebagai karsinomapayudara invasif. Sampel dipulas dengan teknik immunohistokimia menggunakan CD-10; dan kemudianekspresi CD-10 dianalisis dengan uji Kruskal-Wallis.HasilBerdasarkan analisa statistik Kruskal-Wallis terhadap 60 sampel kasus karsinoma payudara invasif mengujihubungan ekspresi imunohistokimia CD-10 dengan grading histopatologi karsinoma payudara invasif, diperolehp-value=0,0001 (p<0,05).KesimpulanEkspres

Hubungan Jumlah Sel Mast dengan Tampilan Ekspresi Vascular Endothelial Growth Factor (VEGF) pada Cutaneous Squamous Cell Carcinoma Delyuzar; Soekimin; Edi Kerina Sembiring,
Majalah Patologi Indonesia Vol 28 No 3 (2019): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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BackgroundThe most common non-melanocytic skin cancers are basalioma and squamous cell carcinoma. Squamouscell carcinoma is a malignancy of squamous epithelium. Mast cells stimulate neovascularization andangiogenesis in multiple tumors. One of the main factors of angiogenesis in squamous cell carcinoma isthe Vascular Endothelial Growth Factor (VEGF). The aim of this study is to investigate the associationbetween number of mast cells with the expression of VEGF of cutaneous squamous cell carcinoma.MethodsThis is an analytical descriptive study with cross sectional approach. The sample of this study are paraffinblocks of skin tissue which diagnose histopathologically as squamous cell carcinoma. Then the sampleswill be stained with immunohistochemistry CD117 (Novocastra®) to count the number of mast cells andVEGF (Dako®) to see angiogenesis. Their expression will be analyzed with Fisher’s Exact Test statisticalanalysis to investigate the association between number of mast cells with expression of VEGF ofcutaneous squamous cell carcinoma.ResultsThe number of mast cells ≥15 cells were 7 cases (23.3%). Expressed VEGF positive expression in 30cases (100%). Suitability test Fischer's Exact Test has value not significant (p> 0.05).ConclusionThere is no conformance relationship between the number of mast cells with VEGF expression of p-value.

Hubungan Ekspresi Imunohistokimia Cyclooxygenase-2 (COX-2) dengan Derajat Histopatologi Meningioma Delyuzar; Indri Mahrani; Joko S. Lukito
Majalah Patologi Indonesia Vol 28 No 3 (2019): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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ABSTRAKLatar belakangMeningioma adalah neoplasma intrakranial primer yang umum pada orang dewasa, berasal dari sel arachnoid(meningothelial). Gambaran histologis meningioma adalah prediktor penting untuk perilaku tumor.Cyclooxygenase-2 (COX-2) adalah enzim yang diekspresikan dalam berbagai jenis tumor dan diketahui terkaitdengan perilaku ganas tumor. Tujuan dari penelitian ini untuk mengevaluasi ekspresi imunohistokimia COX-2pada meningioma dan mencari hubungannya dengan derajat histopatologik.MetodePenelitian Ini merupakan penelitian deskriptif analitik dengan pendekatan cross sectional. Sampel penelitian iniadalah 22 blok parafin dengan diagnosis meningioma yang ditentukan derajat histopatologiknya dan diwarnaidengan imunohistokimia COX-2. Penilaian dilihat berdasarkan intensitas warna dan persentase sel yangterwarnai pada sitoplasma. Intensitas warna: 1 (lemah), 2 (sedang), 3 (kuat). Persentasi sel yang positif=0(0%), 1 (<10%), 2 (10-50%), 3 (>50%). Penilaian merupakan penjumlahan intensitas warna dan persentasipositif (>4), negatif (<4).HasilDuapuluh dua kasus meningioma: usia rerata=41 tahun, perempuan=90,9%, meningothelial=22,7%, derajatI=77,3%). Ekspresinya. COX-2: negatif: 17 kasus (77,3%) semuanya merupakan derajat I. COX-2 positif: 5kasus (22,7%) (4 kasus derajat II, 1 kasus derajat III). Ekspresi COX-2 pada meningioma berhubungan denganderajat histopatologik berdasarkan WHO (p=0,001).KesimpulanPada penelitian ini terdapat hubungan antara ekspresi imunohistokimia COX-2 dengan derajat histopatologik, dimana COX-2 lebih terekspresi pada derajat II dan III.

Hubungan Ekspresi CD117 dan Epidermal Growth Factor Receptor (EGFR) terhadap Grading Histopatologi Adenoid Cystic Carcinoma pada Kelenjar Liur Delyuzar; Ren Astrid Allail Siregar; T. Ibnu Alferraly
Majalah Patologi Indonesia Vol 29 No 1 (2020): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

BackgroundTend to have adenoid cystic carcinoma (AdCC) is one of malignant epithelial tumor of the salivary gland poor prognosis, localrecurrence and metastases. Histopathological grading system according Weert, et al. observed from presence or absence solidcomponent of AdCC. CD117 is a transmembrane tyrosine kinase receptor usually strong expression in AdCC. EGFR belongs to theErbB family of receptor tyrosine kinases associated with cell proliferation, angiogenesis and metastases. Patients with advanceddisease may benefit from targeted therapy besides conventional management. Expression of CD117 and EGFR may give proposaltherapy through difference histology pattern of AdCC based on histopathological grading.MethodsThis is an analytical descriptive study with cross sectional approach. Formalin-fixed paraffin-embedded sections from 35 adenoidcystic carcinomas were obtained for histopathological grading and for immunostained of CD117 and EGFR using monoclonalantibodies. The staining intensity and percentage of cells with membranous and cytoplasmic of CD117 and EGFR expression werescored and then grouped according to the parameters of the Allred scoring system.ResultsChi-square test between CD117, EGFR expression and histopathological grading of AdCC showed no sigficant differences.Besides, McNemar test between CD117 and EGFR expression and histopathological grading AdCC showed significant differences,p-value=0.03 (p<0.05).ConclusionsCD117 and EGFR could be used to detected salivary gland AdCC

Identifikasi Polimorfisme Gen CYP1A1 pada Karsinoma Sel Skuamosa dan Adenokarsinoma Serviks Delyuzar; Hidayat; Hilda Fitriyani
Majalah Patologi Indonesia Vol 29 No 2 (2020): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

BackgroundCervical cancer is the third most common cancer in women with risk factor of smoking, high parity, long term use of oralcontaception that are associated with chemical carcinogenesis. Chemical carcinogenesis require biotransfor-mation of lipophilicsubstrates to hydrophilic metabolites, therefore facilitating their secretion from the human body. Cytochrome P450 (CYP) is one ofgenes that have important role in this process. Benzo[α]pyrene and estrogen have a common biotransformation process which ismetabolized by CYP, particularly CYP1A1. The objectives to identify the frequency and distribution of CYP1A1 gene polymorphismin squamous cell carcinoma and adenocarcinoma of the cervix.MethodsThis is an analytical descriptive study with cross sectional approach. CYP1A1 gene polymorphism (3801T/C or Ile462Val) wasanalyzed using PCR-RFLP method followed by gel electrophoresis.ResultsCYP1A1 gene polymorphisms (3801TC) in squamous cell carcinoma were 50% heterozygote T/C, 36% wild-types T/T and 14%homozygote C/C. CYP1A1 gene polymorphisms (3801TC) in adenocarcinoma were 60% heterozygote T/C and 40% wild-types T/T.CYP1A1 gene polymorphisms (Ile462Val) in squamous cell carcinoma were 97.2% heterozygote Ile/Val, and 2.8% homozygoteVal/Val. CYP1A1 gene polymorphisms (Ile462Val) in adenocarcinoma were 100% heterozygote Ile/ValConclusionThe most common type of CYP1A1 gene polymorphism (3801TC and Ile462Val) in squamous cell carcinoma and adenocarcinomaof the cervix were heterozygote.

Analisis Gambaran Morfologi Limfadenitis Tuberkulosis Menggunakan Metode Biopsi Aspirasi Jarum Halus dan Polymerase Chain Reaction Delyuzar; Soekimin; Dedy Suryadi
Majalah Patologi Indonesia Vol 29 No 2 (2020): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

BackgroundIndonesia is the 2nd largest of tuberculous (TB) cases in the world. Therefore, TB must be controlled by increasing the capacity anddeveloping universal access needed for TB diagnose accurately and quickly. Progression in controlling TB and decreasing theconsequence can be accelerated through early detection and promt treatment. This study used PCR test as gold standard, albeitthe high cost. Thus, quick, accurate and low cost diagnostic method is needed. In this case morphologic features requirementinclude the existence of epithelioid and necrosis in FNAB smears. Objective was to analyze morphologic features of tuberculosislymphadenitis using FNAB and PCR methods.MethodsThis study is a cross sectional analysis. N samples are all kind of lymphadenitis tuberculosis. Morphologic features in FNABcytology must be confirmed using PCR test and performing chi-square test respectively.ResultsEpitheloid and necrosis features of TB lymphadenitis on FNAB showed 100% positive on PCR methods. This study divided intothree groups. Statistic analyses between group I and II, group II and III showed in significant differences (p=0.074); while betweengroup I and III showed significant differences (p<0.05).ConclusionsPCR method could confirmed morphologic features (epitheloid and necrosis) of TB lymphadenitis

The Presence of Helicobacter pylori in cases of Chronic Tonsillitis in Medan Harahap, Rini Syahrani; Delyuzar; Jessy Chrestella
Sumatera Medical Journal Vol. 3 No. 1 (2020): Sumatera Medical Journal (SUMEJ)
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/sumej.v3i1.2044

Background: Helicobacter pylori is a well known pathogen which is recognized as the most frequent infection of individuals. Helicobacter pylori plays important role in the etiology og gastritis, gastric and duodenal ulcers. We investigated the presence of Helicobacter pylori as an extragastric reservoir in the tonsilectomy specimens. Aim: To determine the presence of Helicobacter pylori in cases of chronic tonsillitis in tonsilectomy specimens. Methods: This is a descriptive study with cross sectional which the sample will be obtained using consecutive sampling method. We collect 43 cases with chronic tonsilitis were examined in tonsillectomy specimens for which available tissue for routin staining could be retrieved were histopathology chronic tonsillitis. The colonization of the helicobacter pylori have been evaluated with hematoxylin & Eosin and Giemsa stains under the light microscope. Results: With H&E staining, Helicobacter pylori has been detected in 23 cases (53,48%) of the tonsil-lectomy specimens in total. No colonization has been observed in the remaining 20 cases (46,51%). With giemsa staining Helicobacter pylori has been detected in 29 cases (67,74%) of the tonsillectomy specimens in total. No colonization has been observed in the remaining 14 cases (32,55%). Keywords: Helicobacter pylori, Chronic Tonsillitis, H&E, Giemsa.

Nilai Numerik Sitomorfometri Keliling dan Densitas Inti pada Fibroadenoma, Karsinoma Duktus dan Karsinoma Lobular Payudara Delyuzar; fitriani Lumongga; Muhammad Nadjib D Lubis
Majalah Patologi Indonesia Vol 19 No 1 (2010): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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ABSTRAKLatar BelakangPemeriksaan sitologi biopsi aspirasi jarum halus penting dalam diagnosa preoperatif lesi payudara. Sampai saatini interpretasi sediaan sitologi sering bersifat subjektif. Untuk mendapatkan diagnosa yang lebih objektif,diperlukan aplikasi tambahan dengan menggunakan komputer melalui images analysis. Morfometri yangdilakukan dengan komputerisasi dan analisa gambar dapat digunakan untuk menghitung ukuran sel, sepertikeliling inti (perimeter) dan densitas inti.TujuanTujuan studi ini membandingkan ukuran perimeter dan densitas inti antara fibroadenoma, karsinoma duktus dankarsinoma lobular payudara dengan menggunakan image analysis.Bahan dan CaraPenelitian menggunakan studi potong lintang dengan jumlah sampel 10 pada setiap kelompok. Sediaan hasilbiopsi aspirasi diwarnai Diff-Quik dan dilakukan pengukuran morfometri dengan pewarnaan Fuelgen. Penilaianmorfometri inti menggunakan MCID Analysis software pada 50 sel tiap slide dan dinyatakan dengan angka yangdapat dipindahkan ke dalam bentuk histogram. Perbedaan beberapa variabel diuji statistik dengan tesBonferroniHasilRata-rata keliling inti fibroadenoma mammae: 0,513 µm, karsinoma duktus : 0,887 µm, karsinoma lobular: 0,634µm. Rata-rata densitas inti fibroadenoma 0,45006: Relative optical density (ROD) dan karsinoma duktus: ROD.0,5338KesimpulanPada hasil penelitian terdapat perbedaan rata-rata keliling inti pada lesi fibroadenoma, karsinoma duktus dankarsinoma lobular payudara serta terdapat perbedaan densitas inti lesi payudara fibroadenoma dan karsinomaduktus dengan menggunakan pewarnaan Fuelgen.

Ekspresi AgNOR dan Ekspresi Ki-67 pada Grading Adenokarsinoma Prostat Delyuzar; Roza Rita; Lidya Imelda Laksmi
Majalah Patologi Indonesia Vol 29 No 3 (2020): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

BackgroundProstate cancer is the second most common cancer and fifth most common cancer cause of death in males. Tissue biopsy is a goldstandard examination to diagnose prostate cancer. One of the hallmarks of cancer is increased activity of cell proliferation. Thisactivity can be detected with Ki-67 and AgNOR (Argyrophilic nucleolar organizing region). The aim of this study is to analyzedcorrelation between AgNOR and Ki-67 expression in grading of prostated adenocarcinoma.MethodsThis analytic cross-sectional study was held in Laboratory of Anatomical Pathology of Medical Faculty of USU/ RSUP H. AdamMalik Medan. Thirty paraffin blocks diagnosed with prostate adenocarcinoma were stained with H&E and p63 immunohistochemistrythen evaluated based on Gleason’s histopathological grading and stained with Agnor and Ki 67.ResultsAgNOR expression yang diperoleh pada grading adenokarsinoma prostat group 1, 2, 3, 4, dan 5 berturut-turut adalah 43; 32,40(±14,54); 64,29 (±28,2); 59,5 (±28,32); 69,22 (±29,26). Ekspresi Ki-67 pada setiap grading adenokarsinoma prostat group 1, 2, 3, 4,dan 5 secara berurutan adalah 43; 32,4 (±14,53); 64,29 (±28,2), 59,5 (±28,31); 69,22 (±29,26). Statistical analyses showed thatthere was no significantly correlation between grading of prostate adenocarcinoma and AgNOR expression (p=0.065), and Ki-67expression (p=0.18). Nevertheless, a significantly correlation between KI-67 expression and grading of prostate adenocarcinomawas found (p=0.34).ConclusionKi-67 could be used as prognostic indicator for prostate adenocarcinoma.

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