Andyanita Hanif Hermawati
Department Of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

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Daya hambat kombinasi madu mangga (Mangifera indica)-susu probiotik terhadap pertumbuhan Eschericia coli ATCC 6538 dan Staphylococcus aureus ATCC 8739 Andyanita Hanif Hermawati; Aryati Aryati; Isnaeni Yudi Haryanto
Pharmaciana Vol 6, No 2 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.437 KB) | DOI: 10.12928/pharmaciana.v6i2.3741

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Growth inhibitory activity of combination of mango honey-probiotic milk produced by Lactobacillus acidophilus (L.a) and Lactobacillus casei (L.c) against E. coli and S. aureus has been studied. The fermented milk was prepared by inoculating each probioticin milk at 43ºC and then incubated at room temperature for 24 hours. Diffusion agar technique by using nutrient agar media was used to assess the minimum inhibition concentration (MIC). Result of the research showed that pH of fermentation broth of the probiotic milk reached 5. Biomass obtained that calculated by Total Plate Count of L.a and L.c was 1.3 x 107 cfu/mL and 20.7 x 1013 cfu/mL respectively. Inhibition zone diameter ofthe mangohoney-L.c showed an optimum antimicrobial effect against tested bacteria at 1:9 ratio. The MIC value against E. coli and S. Aureus was 15.7 ± 6,1 mm and 13.3 ± 3.0 mm respectively. The activity of mango honey-L. aexhibite dan optimum antimicrobial effect against tested bacteria at 2:8.The MIC value againstE. coli and S. Aureuswas14,7 ± 1,1mm and 13.3 ± 1.3mm respectively.
Uji Kualitatif Merkuri pada Krim Pemutih Wajah yang Tidak Terdaftar Badan Pengawas Obat dan Makanan di Kabupaten Tulungagung Andyanita Hanif Hermawati; Qurrotu A’yunin Lathifah
Borneo Journal of Medical Laboratory Technology Vol 1 No 2 (2019): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.244 KB) | DOI: 10.33084/bjmlt.v1i2.712

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The cream used to brighten up the skin and fades freckles of the face is called whitening cream. Mercury is the active ingredient that is commonly used in face whitening cream. The use of hazardous substances in cosmetics namely mercury. Irritation, nervous disorders, itching, until skin cancers are the result of the use of mercury in whitening cream. This research aims to find out whether there is mercury or not by qualitative test in whitening cream which is unregistered by Badan Pengawas Obat dan Makanan (BPOM) in Tulungagung. The type of this research is descriptive research. The number of whitening cream samples which is unregistered by Badan Pengawas Obat dan Makanan (BPOM) Tulungagung as many as six samples. Colour test used 0.5 N KI solution is a qualitative test of mercury. Results of the study with a qualitative analysis show that all negative samples contain mercury.
Uji Kuantitatif Kadar Protein pada Tempe Kedelai dan Lamtoro Qurrotu A’yunin Lathifah; Andyanita Hanif Hermawati
Borneo Journal of Medical Laboratory Technology Vol 2 No 1 (2019): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.343 KB) | DOI: 10.33084/bjmlt.v2i1.1089

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Food is the most essential requirement for humans to maintain life and living. One of the quality of food can be from nutrients contained in it. Efforts to diversify food can be done by finding new food ingredients or materials from existing food and developed into a diverse food material. The use of lamtoro seeds as the basis for making tempeh is one form of food diversification. Sampling in this study is Simple Random Sampling with a total sample of 2 soybean tempeh and 2 lamtoro tempeh. Research data collection using experiments. In this study, different results were obtained from each soybean tempeh and lamtoro tempeh.
Review: Gambaran Nikotin pada Perokok Pasif di Kabupaten Tulungagung Qurrotu A’yunin Lathifah; Andyanita Hanif Hermawati; Anggi Yupita Putri
Borneo Journal of Medical Laboratory Technology Vol 3 No 1 (2020): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v3i1.1594

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Passive smokers do not have a smoking habit but inhale cigarette smoke exhaled by those around them who smoke. Exposure to cigarette smoke, one of which nicotine, can trigger harmful substances to the body. This study aimed to analyze the presence or absence of nicotine in passive smokers in Tulungagung Regency. The method used is descriptive qualitative with a sample of 30 urine of passive smokers. The results of the examination showed negative nicotine (93%) with the criteria of the female sex (77%), 11-20 years old (20%), 11-20 years of exposure (37%), and the level of elementary school education (37%). Respondents who were positive for nicotine were 2 people (7%) with characteristics of male sex (7%), aged 31-40 years (7%), long exposure for 31-40 years (7%), and level education of elementary school and junior high school (3.3%). This study concludes that nicotine negative respondents (93%). Nicotine is hazardous for the health of passive smokers. The longer exposure to cigarette smoke, the higher the nicotine content in the body so that the body's nicotine content can be detected. People are advised to use masks and avoid exposure to cigarette smoke too often to prevent the entry of harmful substances into the body
Review: Perbedaan Kadar Hemoglobin Menggunakan Hematologi Analyzer dan Spektrofotometer pada Ibu Hamil Andyanita Hanif Hermawati; Eka Puspitasari; Deny Yusnia Milasari
Borneo Journal of Medical Laboratory Technology Vol 3 No 2 (2021): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v3i2.2388

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Hemoglobin is the main component of red blood cells, a conjugated protein that functions to transport oxygen and carbon dioxide. Hemoglobin examination can be done using a hematology analyzer and spectrophotometry. This study aims to determine the data analysis whether there is a difference in hemoglobin levels using a hematology analyzer and spectrophotometry in pregnant women. The method used is Literature Review; the nature of this research is descriptive. The population of this research is health journals that have been through screening, namely journals of differences in hemoglobin levels using a hematology analyzer and spectrophotometry in pregnant women. In this study, the data used are secondary data and data analysis using qualitative data. The results of the evaluation of the study by the Literature Review with hemoglobin levels in pregnant women at Hermina Hospital, Surakarta, showed that the average hemoglobin level of 30 samples of pregnant women using a hematology analyzer was below normal, namely 10.3 gr/dL and normal hemoglobin level is 12.80 gr/dL. The hemoglobin level using spectrophotometry and hematology analyzer on the sample was immediately checked, the results of the hemoglobin levels were 12.98 gr/dL and 11.70 g/dL, respectively. The hemoglobin level showed that the average hemoglobin before being given Fe-Folate with orange water was 11.71 g/dL. The conclusion of this study is the difference in hemoglobin levels is not much different, so there is no difference between the spectrophotometry and the hematology analyzer
Uji Reduksi Methylene Blue pada Susu Segar di Kelompok Peternak Sapi Perah Dusun Pabyongan Kabupaten Tulungagung Andyanita Hanif Hermawati; Hariyanto Hariyanto; Aulia Zahratul Husna
Borneo Journal of Medical Laboratory Technology Vol 4 No 1 (2021): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v4i1.2954

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Milk is a food that has complete and perfect nutrition. The main product produced by dairy cows is livestock. In Tulungagung there are several dairy farming areas, including the Pabyongan hamlet. The purpose of this study was to analyze the reduction test using methylene blue 1% in fresh milk in the dairy farmer group of Pabyongan Tulungagung hamlet. This research is a qualitative descriptive design with the Simple Random Sampling technique. The results of this study indicate that from a total of 10 fresh milk samples, it is known that 9 fresh milk samples (90%) have a good reduction test and 1 fresh milk sample (10%) has a bad reduction test. Based on the results of the above research, it can be stated that 90% of the fresh milk samples from the dairy farmer group in Pabyongan Pagerwojo village have a good reduction test. Poor reduction test results are due to the condition of the cage is not clean, the condition of the animal being sick, the condition of the farmer being sick, or the equipment is not clean.
Low density lipoprotein (LDL) in type 2 diabetes mellitus Andyanita Hanif Hermawati; Eka Puspitasari; Cholila Lailatul Nurmala
Medical Laboratory Analysis and Sciences Journal Vol 1 No 1 (2019): May 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.065 KB) | DOI: 10.35584/melysa.v1i1.19

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Hyperglycemia due to abnormal insulin secretion, insulin work or both. Type 2 Diabetes Mellitus is the most among other types of diabetes mellitus. Diabetes mellitus is usually accompanied by dyslipidemia. Dyslipidemia is a disorder of lipid metabolism or cholesterol characterized by an increase or decrease in lipid fraction in the plasma. Diabetes mellitus and elevated Low-Density Lipoprotein (LDL) cholesterol levels are often found to be interrelated. The purpose of this study was to describe Low-Density Lipoprotein (LDL) levels in patients with type 2 diabetes mellitus. The research design used was descriptive non-analytic. The population in this study were all patients with type 2 diabetes mellitus using a purposive sampling technique of 30 samples. Data collection was carried out by observing the medical record data of type 2 diabetes mellitus patients with Low-Density Lipoprotein (LDL) profile data for 2017. The results showed that patients with type 2 Diabetes Mellitus suffered the most by male sex with a percentage of 57%, aged over 45 years with a percentage of 97%, and having high LDL levels as much as 40%.
Inhibitory Activity of Probiotic Milk Against Eschericia coli ATCC 6538 and Staphylococcus aureus ATCC 8739 Andyanita Hanif Hermawati; Aryati Aryati; Isnaeni Isnaeni
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 1 (2020): JUNI
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (549.674 KB) | DOI: 10.20473/bikfar.v7i1.29059

Abstract

The aim of this research is to evaluate inhibitory activity of Lactobacillus acidophilus (L.a) and Lactobacillus casei (L.c) probiotic milk against Escherichia coli and Staphylococcus aureus growth. The fermented milk was prepared by inoculating each probiotic in milk at 43ºC and then incubated at room temperature for 24 hours. Diffusion agar method by using nutrient agar media was used to assess the minimum inhibition concentration (MIC). Obtained results showed pH of fermentation probiotic milk 5 + 0,05. Optimum inhibition zone diameter of L. acidophilus fermented milk against Escherichia coli and Staphylococcus aureus at MIC value of 14 mm. Inhibition zone diameter of L. casei fermented milk obtained an optimum against Escherichia coli and Staphylococcus aureus at MIC value of 12 mm and 13 mm respectively. The L. acidophilus and Lactobacillus casei probiotics fermented milk exhibited considerable antibacterial agent against Escherichia coli and Staphylococcus aureus.
Penetapan Vitamin C dalam Jeruk Keprok Segar dan Jus secara Iodometri: Determination of Vitamin C in Fresh Tangerines and Juice by Iodometry Qurrotu A’yunin Lathifah; Dora Dayu Rahma Turista; Andyanita Hanif Hermawati; Riska Miftakhul Jannah
Borneo Journal of Medical Laboratory Technology Vol. 4 No. 2 (2022): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v4i2.3313

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Orange has high levels of Vitamin C. The most frequently consumed orange is tangerine. People usually consume fresh oranges and juice. This study aimed to determine the difference in Vitamin C content in fresh tangerine and tangerine juice. The research design used was experimental. Population in this study area includes all citrus fruits sold in the Ngemplak Tulungagung market. The sample was obtained by using the purposive sampling technique. The data obtained are fresh tangerine has a content of Vitamin C as much as 26,4% and tangerine juice as much as 7,2%, then data is processed with the software SPSS 16 and analyzed by Independent T-test. Result of Independent T-test is obtained P-value = 0.001 < α = 0.05.Based on the result of the Independent T-test, it can be concluded that H0 is rejected, so there is a difference in Vitamin C content in fresh tangerine and tangerine juice on an Iodometric basis.
Pengaruh Serum yang Disimpan Selama Lima Hari Suhu 2-8°C dengan Serum yang Diperiksa Langsung pada Pemeriksaan Kolesterol Total: The Effect of Serum Stored for Five Days Temperature of 2-8°C with Serum That is Checked Directly on Total Cholesterol Testing Chalies Diah Pratiwi; Hariyanto Hariyanto; Andyanita Hanif Hermawati; Intan Niawati Fajrin
Borneo Journal of Medical Laboratory Technology Vol. 4 No. 2 (2022): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v4i2.3790

Abstract

Examination of blood cholesterol at this time became the trend of laboratory examination that much in demand by most people because of many diseases caused by hypercholesterolemia due to wrong diet or high cholesterol levels. Damaged equipment, reagent exhaustion, a large number of samples, laboratory distance with the sampling site too far, and power outages resulted in delayed laboratory examination that required sample storage. This study aimed to determine the effect of serum storage for five days with a serum examined directly to examine total cholesterol. The research design used was an experiment. The population in this research is all female students; Level 3 Study Program D3 Health Analyst STIKes Hutama Abdi Husada Tulungagung, using the Purposive sampling technique, counted 15 respondents. This research was conducted in May 2018. The data obtained were processed using SPSS 16 software and analyzed by Paired t-Test. This study showed the average value of total cholesterol levels in serum examined directly at 163 mg/dl while stored for five days temperature of 2-8 ° C at 158 mg/dl. After the paired test obtained value of P-value = 0.000, so P-value = 0.000 <α = 0.05, it can be concluded there is an influence between total cholesterol levels are checked directly and stored for five days temperature 2-8°C. Five days of serum storage affects the results of total cholesterol tests that lead to a decrease in total cholesterol, but the reduction is insignificant.