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ANTAGONIS BAKTERI PROBIOTIK YANG DIISOLASI DARI USUS DAN LAMBUNG IKAN KERAPU BEBEK (CROMILEPTES ALTIVELIS) TERHADAP BAKTERI PATOGEN ', Feliatra; Fitria, Yuni; ', Nursyirwani
Jurnal Perikanan dan Kelautan Vol 17, No 01 (2012)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.17.01.%p

Abstract

Grouper fish has a fairly high economic value, especially in Asiancountries, namely Hong Kong, Singapore, Taiwan and China. High marketdemand. On aquaculture, is one important component of fish feed that plays animportant role in the cultivation. Application of probiotics in aquaculture fish feedhas not been done yet in the Fisheries sector. The research was conducted inOctober 2008, Grouper is used as the intestines and stomach samples were takenas a source of probiotics, whereas pathogenic bacteria obtained from thecollection of marine microbiology laboratory of Faculty of Fisheries and MarineRiau University. From the isolation of bacterial strains found 11 strains that havethe potential as probiotics, then proceed to the molecular test by using rDNA16 s.Which is obtained two strains of probiotic bacteria Bacillus cereus and the speciesof bacteria Strenotrophomonas maltophilia. The test results found that theantagonist Bacillus cereus isolates to inhibit the growth of pathogenic bacteriaAeromonas sp and two species of Vibrio sp. While the bacteriaStrenotrophomonas maltophilia showed no antagonism against pathogens.
Production, Decomposition Rate and Identification of Bacteria on Avicennia alba Litter in the Coastal Zone Kuala Indragiri Riau Province Simanjuntak, Ida Rahayu; Yoswaty, Dessy; ', Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract

A research on the production, decomposition rate and identification of bacteria on Avicennia alba litter in the coastal zone Kuala Indragiri, Riau Province. This research was aimed to observe the contribution of mangrove forests in the productivity for surrounding environment and conducted from December 2014-March 2015. The litter production was calculated by using litter-trap. Sample was collected once in every 15 days during 45 days of observation. The litterfall was oven-dried and weighed. The result showed that avarage production of litterfall of A. alba in all station was 297.696 g/m2/45days (fresh weight) and 189.763 g/m2/45days (dry weight). The average total shrinking dry weight of litterfall leaf /day was at 0.13225 g/day or 1.3225 %/day. The average reduction dry weight of A. alba litterfall in every station was 7.848 g during 45 days of observation with a percentage value of the decomposed litterfall weight of 78.480% or 0,523 g/day, with final result of litterfall dry weight is 2,152 g. Seven types of bacterial colonies were identified on each station and consisted of Bacillus sp (2), Micrococcus sp, Xanthomonas sp, Pseudomonas sp, Vibrio sp and Escherichia sp.Keywords: Avicennia alba, litter, production, decomposition rate, bacteria, Kuala Indragiri
ISOLATION OF PROBIOTIC BACTERIA CANDIDATES ISOLATED FROM BARRAMUNDI (Lates calcarifer) ANTAGONISTIC AGAINTS Vibrio alginolyticus Yoswaty, Dessy; ', Afrimansyah; ', Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract

Probiotic is an alternative subtance to prevent fish diseases which is environmentally friendly. The research purpose was to isolate potential probiotic to control bacteria pathogen in barramundi (Lates calcarifer) to control the bacteria pathogen. The probiotic bacteria candidates were isolated from intestine of barramundi by using the spread and streak methods on MRS and TSA media. The antibacterial activity was examinined by using disc diffusion agar method on Zobell 2216E medium. The results showed thirty eight isolates were found which consisted of coccus, white, orange and yellow colonies. Twelve isolates had inhibition against Vibrio alginolyticus,two isolates showed highest inhibition zone and antivibrio activities. The highest inhibition zone (10.04 ± 1,48 mm) and (9,50 ± 2,12 mm) and the antivibrio activities (4.04 mm) and (3.50 mm) were indicated by KPBU2-5 and KPMU2-6 isolates respectively.Keywords: Probiotics, Isolation, Barramundi, Antagonistic.
ISOLATION HETEROTROPHIC BACTERIA ON SEDIMEN IN WATERS CAPE DISTRICT MEDANG NORTH RUPAT RIAU PROVINCE AND THE ACTIVITY AGAINST PATHOGENS BACTERIA (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas sp) Sari, Desy Mutia; Yoswaty, Dessy; ', Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract

Heterotrophic bacteria in marine ecosystems have important as decomposer of organic material into the elements essential. This group of bacteria can be found in a variety of habitats including marine sediment. This research aims to isolate the heterotrophic bacteria and the activity against pathogenic (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas sp). The research was conducted from November until December 2016. Samples were obtained from the waters of Tanjung Medang, District of North Rupat, Riau Province. Isolation and antagonistic test were conducted at the Laboratory of Marine Microbiology and Biotechnology Department of Marine Sciences Faculty of Fisheries and Marine. In this research was a survey method where heterotrophic bacteria from the sediment were isolated on marine agar medium by pour plate and streak quadran techniques. Activity of the heterotrophic isolate against bacterial pathogens was conducted using diffusion agar on Mueller Hinton Agar (MHA). The number of heterotrophic bacteria ranged from 4,4 × 106 up to 13,5 × 106 CFU/g.Twelve isolates of the sediment bacteria were selected for the antagonism test. However, only nine isolates (Isolate A, C, E, F, O, S, V, W, Z) were able to inhibit the growth of pathogenic bacteria. One isolate (E) performed the highest ability to inhibit the growth of patogens (V. alginolyticus, A . hydrophila and Pseudomonas sp).Keywords: Isolation, Heterotrophic, Sediment, Antagonism, Pathogenic Bacteria.
Effect of stocking density on Growth and Survival rate Mangrove Shells, Polymesoda expansa, seeds in Dumai Coastal Waters of Riau Province. Asri, Zainul; Nasution, Syafruddin; ', Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract

This research was conducted in November-December 2016 in the coastal waters of Dumai, Riau Province. This research aims to determine the growth and survival rate of seeds of mangrove shells P. expansa in natural habitats with different densities. This research uses experimental method. This research consists of one treatment that is with stocking density of three levels of 20, 25 and 30 individuals / container. The container used is a pond made of net (2 mesh size) with a size of 50x50 cm. The results showed that the best growth was found in the treatment with the density of 20 individuals / containers. Also, the best survival rate was found in the treatment of 20 individuals / containers of 98.6% size.Keywords : Polymesoda expansa,seeds Growth and Graduation rate, Stocking Density, Dumai.
TOLERANCE OF PROBIOTIC BACTERIAL CANDIDATE FROM KAKAP PUTIH ON pH AND BILE SALT Harjuni, Fiki; Mulyadi, Aras; ', Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 3, No 2 (2016): Wisuda Oktober Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract

The use of probiotic is one of alternatives to solve disease problem in aquaculture. This research aimed to examine tolerance of three lactic acid bacteria (KP1, KP2, and KP3) isolated from kakap putih (Lates calcarifer) toward pH and bile salt at different consentration. The bacterial isolates were enriched on MRS agar medium. Tolerance to pH was conducted in MRS broth at pH 3, 5 and 8. While, tolerance to bile salt were performed on MRS agar at consentration of 0, 0,1, 0,2, 0,3, and 0,4 %. Isolate KP3 at pH and incubated for 48 hours showed the highest tolerance, but the highest tolerance to bile salt was indicated by isolated KP2 at bile salt consentration of 0,3 %.Keyword: Tolerance, probiotic, Kakap Putih, pH, Bile Salt.