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All Journal Jurnal Penelitian dan Pembelajaran Fisika Makara Journal of Health Research
Ariani, Rizka
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Education

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Analisis Landasan Ilmu Pengetahuan dan Teknologi Pendidikan dalam Pengembangan Multimedia Interaktif Ariani, Rizka; Festiyed, Festiyed
Jurnal Penelitian Pembelajaran Fisika Vol 5, No 2 (2019)
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.152 KB) | DOI: 10.24036/jppf.v5i2.107439

Abstract

Research conducted includes descriptive research. On descriptive statistics without analyzing and making conclusions that apply to the public. The population in this study are articles and online books analyzed. The samples in this study are some of the articles analyzed, taken according to the topic. Educational institutions, especially school path education, must be able to accommodate and anticipate the development of science and technology. Teaching material should be the result of the development of the latest science and technology, both related to the results of information acquisition, as well as how to obtain that information and utilize it for the community. With the advancement in technology, it is possible to produce interactive learning media that are interesting and fun for teaching and learning. As Suheri (2006) argues that multimedia gives a pleasant impression of helping the learning process in remembering and multimedia learning in the classroom is not a new phenomenon, but advances in computer technology enable multimedia to be developed to present learning material interactively
Molecular Detection for Japanese Encephalitis and Chikungunya Virus as Etiology Agent for Dengue-Like Fever Symptoms Ariani, Rizka; Sudiro, Tjahjani M; Dewi, Beti E
Makara Journal of Health Research Vol. 22, No. 3
Publisher : UI Scholars Hub

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Abstract

Background: Acute fever with dengue-like fever symptoms with headache, rash, arthritis, perifer bleeding like ptechie and rinhorrhea are symptoms that often complained by patient and it may be caused by arboviruses infection. Arboviruses are very endemic in Indonesia, especially Dengue Virus (DENV), Japanese Encephalisitis Virus (JEV), and Chikungunya Virus (CHIKV) that causing similar symptoms. Molecular detection is very important to confirm the etiology for adequate management. Methods: This study investigated the etiological agent using one step real time RT reverse transcription polymerase chain reaction (RT PCR) method and SYBR Green I as fluorescent binding dye reporter. The viruses that were investigated were JEV and CHIKV. Primer design was using online software NCBI. Those primers fulfill the good primer requirements and can be used as material in RT PCR reaction. Results: The optimum temperature for all primers were at 60 °C. The limit detection of JEV primer was 4355 copy DNA every reaction. Cross reactivity between all primers with DENV serotype 2 RNA and false positive result using healthy person sample were not found. Conclusions: This study has optimized condition RT PCR protocol that can be used as diagnostic tools for patient with dengue-like fever symptoms.
Co-Authors Dewi, Beti E Festiyed Sudiro, Tjahjani M