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Ginting, Elvy Like
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Earth & Planetary Sciences

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KARAKTERISTIK MORFOLOGI BAKTERI SIMBION SPONS MENYERUPAI Cribochalina sp DARI PERAIRAN MALALAYANG SULAWESI UTARA Poluan, Gledys Giacinta; Ginting, Elvy Like; Wullur, Stenly; warouw, Veibe; Losung, Fitje Vera; Salaki, Meiske
JURNAL PESISIR DAN LAUT TROPIS Vol 7, No 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.24452

Abstract

Bacteria are found to be highly associated with various marine organisms, such as sponges. Sponges are known as the product of bioactive compounds. However, some of the compounds produced by sponges are obtained by the simbion-bacteria. Therefore this situation enables sponges simbion bacteria to play a major role in producing bioactive compounds that have been isolated from the sponge. The purpose of this study is to isolate and determine the characteristics of morphology of spongy simbion bacteria resembling Cribochalina sp, which was taken from Malalayang waters, North Sulawesi. Sponge simbion bacteria grow on NB media. Initially, a free-breeding colony was being done before bacteria were accrued in the NA media by 2% sponge broth (patent SID201906301) by means of Strike Plate methods. Based on to this study we had isolate five spongy simbion bacteria that resembling the Cribochalina sp. All five of these isolations have different characteristics of morphology in terms of color, shape, size, and elevation.      Keywords : bacteria, isolation, simbionts, sponges 
ISOLASI BAKTERI LAUT DARI PERAIRAN MALALAYANG, SULAWESI UTARA Wondal, Bella; Ginting, Elvy Like; Warouw, Veibe; Wullur, Stenly; Tilaar, Sandra Olivia; Tilaar, Ferdinand Frans
JURNAL PESISIR DAN LAUT TROPIS Vol 7, No 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.24448

Abstract

Marine bacteria have a lot of potential in exploring the enzyme that can be developed, such as a producer of proteorhodopsin, act as hydrocarbon chlorlastic and can degrade oil. This study aims to obtain isolates and can characterize the bacterial morphology. Malalayang Waters is one of the marine bacterial habitats that has potential area to be studied. This study aims to isolate marine bacteria from Malalayang Waters. These marine bacteria first were diluted into sea water before they were grown on Nutrient Agar (NA). Based on the results of this study it was found that marine bacterial isolated were separated based on their morphological characteristics. The dominant morphological characteristics were yellow whites which dominant shape were irregular.Keywords: bacterial, dilution, isolation.  Bakteri laut memiliki banyak potensi yang dapat dikembangkan. Seperti penghasil proteorhodopsin, berperan sebagai hidrokarbonoklastik dan dapat mendegradasi minyak. Perairan Malalayang merupakan salah satu habitat bakteri laut yang belum diteliti. Penelitian ini bertujuan untuk mengisolasi bakteri laut dari Perairan Malalayang. Bakteri laut ditumbuhkan pada media agar + air laut, selain itu bakteri juga dilakukan pengenceran terhadap air laut sebelum bakteri ditumbuhkan pada media Nutrient Agar (NA). Berdasarkan hasil penelitian ini isolat bakteri  laut ditemukan, bakteri tersebut dipisahkan berdasarkan karakteristik morfologinya. Karakteristik morfologi yang dimiliki dominan berwarna putih kuning dan memiliki bentuk yang dominan tidak teratur. Hal ini dapat memperlihatkan perbedaan bakteri laut dari Perairan Malalayang yang tumbuh.Kata kunci: bakteri, pengenceran, isolasi.
STRUKTUR KOMUNITAS HUTAN MANGROVE DI DESA BUDO KECAMATAN WORI KABUPATEN MINAHASA UTARA Tidore, Sarifudin; Sondak, Calvyn FA; Rumengan, Antonius P; Kaligis, Erly Y; Ginting, Elvy Like; Kondoy, Christine
JURNAL PESISIR DAN LAUT TROPIS Vol 9, No 2 (2021): JURNAL PESISR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.2.2021.35236

Abstract

Mangrove forest can grow on the muddy soil, along the coast and around the river estuaries that are affected by the tidal. The mangrove forest has a specific vegetation structure. Study on the structure of the mangrove forest community, was carried out in the Budo Village. Mangrove community structure data was taken using line transect method. A 100 m line was established from the sea perpendicular to the coast. A total of three line transects was made and each line has five 10 x 10 m plots with 20 m distance between plots. Distance between transect is 50m. This study found that three mangrove species Rhizophora apiculata, Sonneratia alba, and Bruguiera gymmorrhiza were found in the three transects during this study. The highest species biodiversity index was found in transect two (H’ = 0.97). R. apiculata has the highest density and relative density 0.05 ind/m2 and 67.57%. Both R. apiculata and S. alba have the highest frequency and relative frequency of 1.00 and 50% respectively. The highest species coverage and relative coverage area is belonging to S. alba in transect two, 25,89 and 63.57%. S. alba has the highest Important value index 140.72 in transect two.Keywords: Budo Village, Mangrove Forest, Community Structure. 
KARAKTERISTIK MORFOLOGI DAN AKTIVITAS ENZIM PROTEASE BAKTERI SIMBION NUDIBRANCH Hengkengbala, Sabrina I; Lintang, Rosita AJ; sumilat, Deiske A; Mangindaan, Remy EP; Ginting, Elvy Like; Tumembouw, Sipriana
JURNAL PESISIR DAN LAUT TROPIS Vol 9, No 3 (2021): JURNAL PESISR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.3.2021.36672

Abstract

One type of enzyme whose application is very widespread is protease enzymes. Microorganisms that have not been widely explored as a source of protease enzymes are bacteria that are in conjunction with Nudibranch. The study aims to identify the morphological characteristics of bacteria, then determine the hydrolysis ability of protease enzymes from bacteria symbiont Nudibranch. Identification of morphological characteristics is done macroscopic, and microscopic with gram staining. Proteolytic activity tests are performed qualitatively by measuring the proteolytic index of bacteria grown on Skim Milk Agar medium. The results of macroscopic identification of symbiotic bacteria with Nudibranch are dominated by irregular colony shapes, undulated edges, growth of the entire colony has a flat height, milky white colony color, buttery texture and opaque bacterial colonies. Based on microscopic identification, two gram-negative isolates are obtained, namely RL.S14, S1.1.1, and gram positive isolates, namely AK.S00, AK.S01, AK.S02, AK.S03, S.PP1, RL.S6, S2.1.5, S2.2.1, S3.3.1, S3.3.4, RL.S10. Nudibranch symbiont Bacterial has colony cells were dominated by the diplobacillus form. The results of the protease activity test of Nudibranch symbiont bacteria showed that 12 positive isolates had proteolytic activity with the highest proteolytic index produced by isolate AK.S00, which was 2.5 mm. Keywords: Nudibranch, symbiotic bacteria, characteristics, protease
Co-Authors Antonius P Rumengan Deiske Adeliene Sumilat, Deiske Adeliene Erly Y Kaligis Ferdinand Frans Tilaar Hengkengbala, Sabrina I Kondoy, Christine Lintang, Rosita AJ Losung, Fitje Vera Mangindaan, Remy EP Poluan, Gledys Giacinta Salaki, Meiske Sandra Tilaar Sipriana Tumembouw Sondak, Calvyn FA Stenly Wullur Tidore, Sarifudin Veibe Warouw Wondal, Bella