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Analisis Kandungan Gizi Beras dari Beberapa Galur Padi Transgenik Pac Nagdong/Ir36 Fitriyah, Dina; Ubaidillah, Mohammad; Oktaviani, Fariza
ARTERI : Jurnal Ilmu Kesehatan Vol 1 No 2 (2020): Februari
Publisher : Puslitbang Sinergis Asa Professional

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.288 KB) | DOI: 10.37148/arteri.v1i2.51

Abstract

Rice that is generally consumed by Indonesian people is that does not have important nutrient such as β-carotene. Therefore, the way to supply β-carotene requirement in staple foods can be done through β-carotene biofortification by genetic engineering of the rice plant. PAC Nagdong/ IR36 is transgenic Golden Rice which can produce β-carotene. The research aimed to determine some nutritional content of PAC Nagdong/ IR36 rice lines that include carbohydrate, amylose, amylopectin, protein, lipid, and β-carotene content. Amylose was measured using the iodine colorimetric method, whereas the contents of amylopectin and carbohydrates were measured using the by difference method. Protein was measured using the Bradford method, lipid was measured using Proctor and Bowen method, while β-carotene content was measured using the colorimetric method. PAC Nagdong/ IR36 rice lines had carbohydrate content between 85,06-90,16%, amylose 12,94%- 20,81%, amylopectin 69,35- 76,17%, protein 7,76-8,52 µg/µl, lipid 0,65-0,90%, and β-carotene 231,61-920,52 µg/g. Carbohydrate, protein, and β-carotene in PAC Nagdong/ IR36 were higher than TN1 rice variety (control). This indicated that PAC Nagdong/ IR36 provided better nutrition. Amylose content of Nagdong/ IR36 PAC rice was low, which donated its potential to produce fluffier rice whereas high β-carotene content could be used as a source of β-carotene that is useful in overcoming vitamin A deficiency and beneficial for health including in preventing degenerative diseases.
PENGARUH CEKAMAN KEKERINGAN TERHADAP EKSPRESI GEN KETAHANAN OSCATA DAN OSAPX1 PADA PADI TOLERAN KEKERINGAN Oktaviani, Fariza; Novita Sari, Irma; Handoyo, Tri; Siswoyo, Tri Agus; Ubaidillah, Mohammad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v8i2.4857

Abstract

Rice is widely cultivated in Indonesia, where one of the problems is drought. Rice plant growth can be inhibited due to a lack of water, which could cause oxidative stress. One mechanism for self-defence involves activating antioxidative genes. This study aims to determine the regulatory response of OsCATA and OsAPX1 resistance genes in rice under drought stress. The rice varieties used were those already pre-tested, including Siak Raya, Sertani 1, Indragiri, IR64. Drought treatments were 0% (control) and 15% PEG 6000. Results indicated that the interaction of rice variety treatment and drought stress had a highly significant effect on the root length and chlorophyll content but no significant effect on the plant height. Also, the gene expression of OsCATA and OsAPX1 increased in rice plants exposed to drought stress. Variety Sertani 1 was recommended due to their high average increase in root length, reduced average plant height and chlorophyll content, as well as increased gene expression in conditions of drought stress. Padi banyak dibudidayakan di Indonesia, di mana salah satu permasalahannya adalah kekeringan. Pertumbuhan tanaman padi dapat terhambat akibat kekurangan air, yang dapat menyebabkan terjadinya stres oksidatif. Salah satu mekanisme pertahanan diri adalah mengaktifkan gen antioksidatif. Penelitian ini bertujuan mengetahui respons regulasi gen ketahanan OsCATA dan OsAPX1 pada padi yang mengalami cekaman kekeringan. Benih varietas padi yang digunakan sudah diuji pendahuluan sebelumnya, antara lain Siak Raya, Sertani 1, Indragiri, IR64. Perlakuan kekeringan yaitu 0% (kontrol) dan 15% PEG 6000. Hasil penelitian menunjukkan interaksi perlakuan varietas padi dengan cekaman kekeringan signifikan terhadap panjang akar, dan total klorofil namun tidak signifikan terhadap tinggi tanaman. Ekspresi gen OsCATA dan OsAPX1 meningkat pada tanaman padi yang mengalami cekaman kekeringan. Tanaman yang direkomendasikan adalah varietas Sertani 1 yang memiliki peningkatan rata-rata panjang akar yang tinggi, penurunan rata-rata tinggi tanaman, dan kandungan klorofil rendah, serta meningkatnya ekspresi gen pada kondisi cekaman kekeringan.