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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Industri Pertanian Jurnal Veteriner CHEMISTRY PROGRESS BIOTROPIA - The Southeast Asian Journal of Tropical Biology Current Biochemistry Journal Pharmascience JURNAL PERTANIAN Journal of Science Innovare FITOFARMAKA: Jurnal Ilmiah Farmasi Jurnal Ilmiah Teknik Industri Indonesian Journal of Applied Research (IJAR) Pharmaceutical And Biomedical Sciences Journal (PBSJ) Jurnal Pusat Inovasi Masyarakat (JPIM) JURNAL AGROINDUSTRI HALAL
Akhmad Endang Zainal Hasan
Department Of Biochemistry, Faculty Of Mathematics And Natural Sciences, IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Social Sciences Transportation Veterinary

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OPTIMASI KONDISI EKSTRAKSI MENGGUNAKAN ENZIM DENGAN RESPONSE SURFACE METHODOLOGY (RSM) TERHADAP EKSTRAK KELOPAK BUNGA ROSELA (Hibiscus sabdariffa L.) Mardiah, Mardiah; Hasanah, Rima Nidaul; Novidahlia, Noli; Hasan, Akhmad Endang Zainal
JURNAL PERTANIAN Vol 9, No 2 (2018): OCTOBER
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (39.242 KB) | DOI: 10.30997/jp.v9i1.1391

Abstract

Ekstrak kelopak bunga rosela banyak dimanfaatkan sebagai bahan baku pada produk pangan maupun non-pangan. Berdasarkan penelian sebelumnya menunjukkan bahwa penggunaan pektinase dan selulase dapat meningkatkan kadar antosianin yang diperoleh dari ekstrak. Pada penelitian ini, dilakukan optimasi kondisi ekstraksi kelopak bunga rosela (Hibiscus sabdariffa L.) menggunakan pektinase dan selulase dengan Response Surface Methodology (RSM) dengan perlakuan konsentrasi penambahan pektinase dan selulase 1:1 (500-1500 ppm), suhu ekstraksi (35-65 oC) dan waktu ekstraksi (30-90 menit). Pengaruh konsentrasi penambahan enzim, suhu dan waktu ekstraksi terhadap rendemen, kadar antosianin dan total padatan terlarut diuji dengan menggunakan central composite design, diperoleh nilai R2 pada ketiga respon sebesar 0,6. Berdasarkan hasil persamaan yang diperoleh pada setiap respon yang dianalisis, kondisi optimum diperoleh pada penambahan konsentrasi enzim 1000 ppm, suhu ekstraksi 50 oC dan waktu ekstraksi 10 menit . Pada keadaan ini diperoleh rendemen sebesar 84,20%, kadar  antosianin 479,70 mg/L dan total padatan terlarut 2,13 oBrix.
KONDISI HATI TIKUS BETINA AKIBAT INDUKSI 7,12-DIMETHYL BENZ(α)ANTHRASEN (DMBA) DAN PENYEMBUHANNYA DENGAN PROPOLIS DAN NANOPROPOLIS INDONESIA Endang Zainal Hasan, Akhmad; Effend, E. Mulyati; Setiyono, Agus; Sandi, Bayu
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 4, No 1 (2014): FITOFARMAKA
Publisher : Universitas Pakuan

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Abstract

Tujuan dari penelitian ini adalah menentukan efek farmakologis propolis dan nanopropolis untuk pengobatan penyakit hati pada tikus betina yang diinduksi senyawa karsinogenik 7,12 - dimetilbenz(α)antasena (DMBA). Penelitian dilakukan dengan mengamati histopatologi dan makroskopik hati pada 28 ekor tikus betina galur Sprague - Dawley. Tikus percobaan dibagi menjadi 7 kelompok perlakuan dengan 6 kelompok yang diinduksi DMBA (Kelompok I- VI ) dan 1 kelompok sebagai kontrol normal. Kelompok I sebagai kontrolnegatif diberi 1 ml NaCl secara injeksi intraperitoneal (ip). Kelompok II - IV diberinanopropolis 8; 32 dan 56 ppm ip. Kelompok V diberi ekstrak ethanol propolis 233 ppm ip,kelompok VI sebagai kontrol positif diberikan doxorubixin ip dan kelompok VII sebagaikontrol normal diberi penyediaan akuades. DMBA diinduksi selama 11 minggu danpengobatan dilakukan 15 minggu. Setiap minggu tikus ditimbang bobotnya dan diperiksaterhadap inisiasi tumor. Hasil penelitian menunjukkan bahwa ekstrak etanol propolis 233 ppmdan nanopropolis konsentrasi 32 dan 56 ppm dapat mempertahankan kondisi optimal hatitikus. Efeknya adalah setara dengan kontrol normal. 
SUPLEMENTASI KELAPA KOPYOR TERHADAP AKTIVITAS SUPEROKSIDA DISMUTASE DAN PATOLOGI ANATOMI HATI TIKUS AKIBAT PERLAKUAN PARASETAMOL Endang Zainal Hasan, Akhmad; Hasim, Hasim; Setiyono, Agus; Winahyu Ariadini, Sekar
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 3, No 1 (2013): FITOFARMAKA
Publisher : Universitas Pakuan

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Abstract

This research aimed at determining of the effects of the induction of paracetamoland kopyor coconut on the activity of superoxide dismutase (SOD) and pathologicanatomy of rat’s liver. Previous research shows that paracetamol caused negative effect to the body while kopyor coconut has the ability to improve human health, related to its capacity to prevent oxidative damage of human body. In this research, the positive effect of kopyor is approached through measurement of body weight and the activity of the total SOD enzyme of animals tested, which are induced by paracetamol and fed with kopyor. Rat’s administered with temulawak were positive control. The result showed that paracetamol treatment using toxic dose was found to reduce body weight of tested animals. Meanwhile, both kopyor and temulawak supplements were able to counter the negative effect of paracetamol, indicated by the increase in the body weight of tested animals. Other results showed that treatments with temulawak and 5 times-dose of kopyor supplement demonstrated a significantly high total SOD activity in the liver as compared to negative control. Furthermore, pathologic observation of the tested animals’ liver showed that 5 time-dose of kopyor treatment was confirmed to improve the recovery of the liver caused by negative effect of paracetamol, better than the negative control.Key words: kopyor coconut, superoxide dismutase, free radical, pathologic
CAMPURAN PROPOLIS DAN GARAM KELAPA SEBAGAI BAHAN ANTIBAKTERI PLAK GIGI MIXED PROPOLIS AND COCONUT SALT AS A DENTAL PLAQUE ANTIBACTERIAL AGENT Endang Zainal Hasan, Akhmad; Henry Adiprabowo, I Made Artika,
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 2, No 2 (2012): FITOFARMAKA
Publisher : Universitas Pakuan

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Abstract

Karies gigi merupakan masalah kesehatan yang umum terjadi di kalanganmasyarakat Indonesia. Faktor yang paling banyak menyebabkan karies gigi adalah plak gigi. Bakteri yang dominan dalam plak gigi adalah Streptococcus mutans. Salah satu bahan antibakteri kariogenik yang biasa dipakai dalam pasta gigi saat ini adalah fluor. Penggunaan pasta gigi berfluor dapat menimbulkan fluorosis yaitu pelemahan email gigi bila dipakai dalam konsentrasi yang berlebihan. Propolis dan garam kelapa merupakan bahan alami yang berpotensi sebagai antibakteri pengganti fluor. Penelitian bertujuan untuk menguji aktivitas antibakteri dari campuran propolis dan garam kelapa dan membandingkan keefektifannya dengan antibakteri NaF yang terdapat dalam pasta gigi komersial. Uji aktivitas antibakteri S. mutans dilakukan dengan metode hitungan cawan yaitu penghitungan jumlah bakteri yang tumbuh di media contoh dalam cawan petri. Propolis kasar diekstrak dengan alkohol dan didapatkan rendemen sebesar 8.52%. Hasil penelitian menunjukkan bahwa campuran propolis 6.25% dan garam kelapa 1 M mempunyai kemampuan paling besar sebagai antibakteri dan dapat menghambat S. mutans lebih baik daripada NaF 0.3%. Keefektifan propolis-garam terhadap NaF 0.3% sebesar 203.88%.Kata kunci : propolis, garam kelapa, antibakteri, antikaries gigi, Streptococcus mutans,
OPTIMASI KONDISI UNTUK RENDEMEN HASIL EKSTRAKSI KULIT MANGGIS (Garcinia mangostana L.) Optimization of Conditions for Yield Extraction of Mangosteen Pericarp (Garcinia mangostana L.) Endang Zainal Hasan, Akhmad; Nashrianto, Husain; Novia Juhaeni, Rani
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 2, No 2 (2012): FITOFARMAKA
Publisher : Universitas Pakuan

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Abstract

The aim of this study is to determine the levels of yield extract of mangosteen pericarp extracted using Response Surface Methodology (RSM) Central Composite Design (CCD) with various concentrations of ethanol (in the range of 45 to 96 %) and duration of microwave heating (in the range 5.8 to 34.1 o C) or Microwave Assisted Extraction (MAE). The yield extract was calculated as percentage to the weight of origin. The results showed that optimum extraction conditions for yield extract were at ethanol concentration of 70% and heating time of 31.5 minutes which resulted in yield extract of 19.45%. More over, under these conditions resulted yield of 19.83%. The yield extract equation of mangosteen pericarp is Y = -73,7883 + 0,5293 X heating time and X 2 1 + 2,4230 X 2 is ethanol concentration in water as solvent. – 0,0084X12 – 0,00173 X2 2 , where X1 is  heating time and X2 is ethanol concentration in water as solvent. Key words : Optimization, Mangosteen, RSM CCD, MAE, yield
FISIKOKIMIA MADU MULTIFLORA ASAL RIAU DAN POTENSINYA SEBAGAI ANTIBAKTERI Escherichia coli DAN Staphylococcus aureus Hasan, Akhmad Endang Zainal; Herawati, Heny; Purnomo, Purnomo; Amalia, Lathifah
CHEMISTRY PROGRESS Vol 13, No 2 (2020)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.13.2.2020.31594

Abstract

Madu merupakan cairan alami yang telah banyak dimanfaatkan oleh masyarakat luas. Tujuan penelitian ini mengidentifikasi karakteristik madu multiflora asal Riau secara fisik maupun kimiawi sesuai dengan standar Nasional serta efektifitasnya terhadap Escherichia coli dan Staphylocccus aureus. Hasil yang diperoleh terdapat 2 madu dari 12 sampel madu memiliki karakteristik sesuai dengan Standar Nasional Indonesia (SNI) yaitu madu PLW1 dan RHU4.  Madu yang terpilih berdasarkan parameter kadar air, HMF, kadar gula pereduksi, serta kadar abu adalah RHU4, PLW1, RHI2 dan RHI1.  Madu RHU4 memiliki kadar air terendah sebesar 16.45% dan madu RHI2 sebesar 20.56%.  Madu RHU4 memiliki kadar abu paling rendah sebesar 0.01% dan tertinggi madu RHI2 sebesar 1.01%.  Madu dengan kadar gula pereduksi terendah adalah madu RHI1 sebesar 69.41% dan tertinggi madu BKL1 sebesar 95.37%.   Madu RHI1 memiliki kadar HMF yang paling rendah yaitu 0.70 dan tertinggi madu RHU1 yaitu 82.67.  Zona hambat paling kecil untuk Staphylococcus aureus yaitu sebesar 7.84 mm pada madu RHI1 20% sedangkan Zona hambat terbesar pada madu RHI2 80% sebesar 19.25 mm.  Zona hambat terkecil Escherichia coli pada madu RHI1 konsentrasi 40% sebesar 1.22 mm, sedangkan zona hambat terbesar pada madu PLW1 60% sebesar 4.58 mm.ABSTRACTHoney is a natural liquid that has been widely used by the public. The purpose of this study was to identify physical and chemical characteristics of multiflora honey from Riau in accordance with National standards and its effectiveness against Escherichia coli and Staphylocccus aureus. The results obtained were 2 honeys from 12 honey samples having characteristics in accordance with the Indonesian National Standard (SNI), namely PLW1 and RHU4 honey. The selected honey based on parameters of moisture content, HMF, reducing sugar content, and ash content are RHU4, PLW1, RHI2 and RHI1. RHU4 honey has the lowest water content of 16.45% and RHI2 honey of 20.56%. RHU4 honey has the lowest ash content of 0.01% and the highest RHI2 honey of 1.01%. The honey with the lowest reducing sugar content was RHI1 honey at 69.41% and the highest was BKL1 honey at 95.37%. RHI1 honey had the lowest HMF content of 0.70 and the highest was RHU1 honey, namely 82.67. The smallest inhibition zone for Staphylococcus aureus was 7.84 mm for 20% RHI1 honey, while the largest inhibition zone for RHI2 80% honey was 19.25 mm. The smallest inhibition zone of Escherichia coli in RHI1 honey with a concentration of 40% was 1.22 mm, while the largest inhibition zone in PLW1 60% honey was 4.58 mm.
Identifikasi gen aroma pada progeni-progeni backcross antara varietas Ciherang dengan Pandan Wangi Djarot Sasongko Hami Seno; Akhmad Endang Zainal Hasan; Tri Joko Santoso; Bram Kusbiantoro; Zainal Alim Mas'ud
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 2 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Marker-assisted PCR has been considered as the most potential method for fragrant selection. RM223 is the only suitable marker to identify mutated badh2 gene of Pandan Wangi. This research applies RM223-assisted PCR in the introgression of fragrant gene (mutated badh2) of Pandan Wangi variety, to engineer non-transgenic fragrant variety with good agronomic traits as those of Ciherang.  Gene introduction was carried out through site-directed crossing; Pandan Wangi was crossed and backcrossed to Ciherang until heterozygot BC5F1, followed by selfing to obtain homozygot BC5F2.  RM223-assisted selection was conducted in each cross and backcross generation. RM223 was able to identify native, mutated and heterozygot badh2 of Ciherang, Pandan Wangi, and their cross/backcross progenies, respectively. Therefore, the introgression of mutated badh2 within progenies were observed, as well as the statues of badh2 gene (native/mutated) and alleles (homozygot/heterozygot). Further backcross and selfing to obtain BC5F2 is in progress.
KSTRAKSI PROPOLIS MENGGUNAKAN CARA MASERASI DENGAN PELARUT ETANOL 70% DAN PEMANASAN GELOMBANG MIKRO SERTA KARAKTERISASINYA SEBAGAI BAHAN ANTIKANKER PAYUDARA Akhmad Endang Zainal Hasan, Djumali Mangunwidjaja Titi Candra Sunarti, Ono Suparno, Agus Setiyono
Jurnal Teknologi Industri Pertanian Vol. 23 No. 1 (2013): Jurnal Teknologi Industri Pertnian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

Propolis adalah zat resin yang dikumpulkan oleh lebah madu (stingless bee dan honey bee) dari berbagai tanaman, memiliki berbagai aktivitas fisiologis seperti antioksidan, induksi apoptosis dan antikanker. Tujuan penelitian ini untuk menentukan kondisi terbaik ekstraksi propolis dan karakterisasinya sebagai bahan antikanker payudara.  Ekstraksi dilakukan dengan teknik maserasi dengan pemanasan gelombang mikro dengan waktu pemanasan 10, 20 dan 30 menit.  Tiga belas contoh propolis dari Pandeglang, Banten Indonesia digunakan untuk uji induksi apoptosis sel Saccharomyces cerevisiae dan analisis data dilakukan menggunakan Response Surface Methodology (RSM). Waktu ekstraksi dan rasio pelarut etanol 70% sarang lebah terbaik yang terpilih dalam menginduksi apoptosis masing-masing selama 27 menit dan sebesar 20, dengan hasil ekstrak propolis sebanyak 12,67%. Selanjutnya, ekstrak propolis mempunyai konsentrasi dalam menghambat sebanyak 50% (IC50), mematikan 50% sel lestari kanker Michigan Cancer Foundation-7 (MCF-7), dan induksi apoptosis S. cerevisiae masing-masing sebesar 75,34 µg. mL-1, 233 µg.mL-1, dan 6,015 µg.mL-1.  Hasil uji efikasi in-vivo terhadap sel kanker akibat induksi 7,12-dimethyl-benz(a)anthracene (DMBA) menunjukkan bahwa konsentrasi propolis 233 µg.mL-1 dapat menyembuhkan jaringan yang rusak akibat tumor. Kata kunci: propolis, induksi apoptosis, MCF-7, RSM, antikanker payudara
Performance of Broilers Chicken Due to The Administration of Raw Propolis Akhmad Endang Zainal Hasan; Ike Agustiani; Olive Wisepti Pratama; Syifa Khaerani; Mutholaah; Muhammad Zulkifli; Dimas Andrianto; Agus Setiyono
Indonesian Journal of Applied Research (IJAR) Vol. 1 No. 2 (2020): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v1i2.48

Abstract

The use of antibiotics as an Antimicrobial Growth Promoter (AGP) for livestock has been banned because antibiotic residues in livestock meat can trigger antibiotic resistance in consumers. Propolis is a natural source that has benefits like antioxidants, antiviral, antifungal, and antibiotic. The purpose of this study was to determine the effectiveness of the addition of feed from the optimal composition of raw propolis to increasing broiler chickens' body weight of broiler chickens. This study used broiler chickens as experimental animals. The chicken were grouped into six groups consisting of two control groups and four treatment groups with different level concentrations of propolis (2, 4, 6, and 8 g/kg feed). After treatment for four weeks, supplementation of raw propolis in the feed had not been able to provide a positive effect on growth performance and protein levels that were not significantly different from controls. Group chickens with propolis additions 6 g/kg feed showed the best results compared to other treatment groups. The group had final weight values, food conversion ratio (FCR), and carcass weight in 1970.67 gram, 1.52, and 1349.83 gram, respectively. The highest percentage of the carcass was shown by group chicken with propolis additions 2 g/kg feed, which is equal to 74.46%. Raw propolis can reduce feed consumption but increase feed conversion ratio.
Children’s Opinion on Vegetables Consumption: A Qualitative Study on School-Agers in City of Semarang Akhmad Endang Zainal Hasan; Laksmi Ambarsari; Karichsa Hariana
Indonesian Journal of Applied Research (IJAR) Vol. 2 No. 2 (2021): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v2i2.129

Abstract

Dates is fruit of palm trees that mostly grow in the Middle East. Dates contain phenolic acids and flavonoids that have antioxidants and potentially inhibit the ability of xanthine oxidase. The purpose of this research to determine the molecular interactions of xanthine oxidase by ligand of phenolic acids and flavonoids to inhibiting the production of uric acid. This research was conducted by site directed docking method. The size of the center of retardation used in this research is x = 26.569, y = 9.985, and z = 113.088 and the retardation volume of x = 14, y = 14, and z = 16. Inhibition by flavonoid and phenolic acid compounds has produces good inhibition strength shown by Gibbs free energy which is negative. The compound with the highest Gibbs free energy value is the anthocyanins compound which is -7.3 kCal / mol, the value is higher than the comparator ligand, allopurinol. Based on the bond analysis that is formed, the best compound in inhibiting xanthine oxidase is syringic acid.
Co-Authors Agus Setiyono Aisyah Aisyah Alfina Zalfa Suryono Amalia, Lathifah Anita Purnamasari Antonius Anre Sianturi Armando Balses Ishimora Aton Yulianto Aziz Syamsul Huda Aziz Syamsul Huda Bayu Sandi Bina Lohita Sari Bram Kusbiantoro Chintia Ayu Puspita Dimas Andrianto DIMAS ANDRIANTO Djarot Sasongko Hami Seno Djumali Mangunwidjaja Dwi Rofifah Putri Jania E. Mulyati Effend Effend, E. Mulyati Ekowati Handharyani Eny Ida Riyanti Eny Ida Riyanti Erlank Bagjavicenna Faisal Rahman Hasanah, Rima Nidaul Hasim Hasim Hasim Hasim Heddy Julistiono Henry Adiprabowo, I Made Artika, Heny Herawati Husnawati Husnawati Husnawati Husnawati I MADE ARTIKA I Made Artika, Henry Adiprabowo Ika Mulawati Purwanti Noviana Ike Agustiani Ilham Gilang Cahya Ahmada Isra Janatiningrum Karichsa Hariana Kristina Mulia LAKSMI AMBARSARI Lusi Agus Setiani Mardiah Mardiah Mega Safithri MEGA SAFITHRI Miko Afrian Moerfiah Mona . Muhammad Zulkifli Mutholaah Nashrianto, Husain Noli Novidahlia Novi Fajar Utami Novia Juhaeni, Rani Nurliani Bermawie Olive Wisepti Pratama Ono Suparno dan Eko Wahyudi Purnomo Purnomo Putri Ramadhanty Rafida Nisa Maghfiroh Ranady Perwira Nuruzzaman Rara Annisaur Rosyidah Rini Kurniasih Sandi, Bayu Sekar Winahyu Ariadini Septa Fitri Handayani Sharon Aurellia Siti Magfiroh Siti Wachidatun Zulaikha Siwi Putri Andini Sri Wahyuni Sri Wardatun Suryani Suryani Syaeful Abidin Syifa Khaerani Titi Candra Sunarti dan Michael (E-Jurnal Agro-Industri Indonesia) Tommy Abrar Tri Joko Santoso Ukhradiya Maharaniq Safira Ulya Utami Vetnizah Juniantito Wiendarlina, Ike Yulia Winahyu Ariadini, Sekar Witdiastuti Witdiastuti Zainal Alim Mas'ud