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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL BIOLOGI INDONESIA
Zein, M. Syamsul Arifin
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Analisis Gen 12SrRNA Dari DNA Mitochondria Kelelawar Pemakan Buah Chironax Melanocephalus (Chiroptera: Pteropodidae) Di Taman Nasional Gunung Halimun Zein, M. Syamsul Arifin; Maharadatunkamsi, Maharadatunkamsi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 1 (2003): February 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (349.089 KB) | DOI: 10.24002/biota.v8i1.2787

Abstract

A study on genetic variation of the high mountain fruit bat (Chironax melanocephalus) was conducted in Gunung Halimun National Park. DNA total from liver tissues were extracted and fragment of the 12SrRNA gene region of mitochondrial DNA were amplified by Polymerase Chain Reaction (PCR). Nucleotide sequence of the PCR products were determined by automated sequencer. Seven haplotype were found among 20 individuals from 6 localities, namely: South Halimun Mountain, Kendeng Mountain, Botol Mountain, Pasir Cangkuang Mountain, Kencana Mountain, and Buligir Putih Mountain. Haplotype diversity and nucleotide diversity were 0.76 and 0.0222. DNA distances values ranged from 0.0028 to 0.0202.
VARIASI GENETIKA KELELAWAR GENUS AETHALOPS (CHIROPTERA: PTEROPODIDAE) DI INDONESIA: TINJAUAN DARI GEN 12SRRNA PADA DNA MITOKONDRIA Maharadatunkamsi, Maharadatunkamsi; Zein, M. Syamsul Arifin
JURNAL BIOLOGI INDONESIA Vol 4, No 2 (2006): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i2.3266

Abstract

ABSTRACTGenetic Variation of Bat in the Genus Aethalops (Chiroptera: Pteropodidae) fromIndonesia: Analysis of 12SrRNA Gene of Mitochondria1 DNA. We report a geneticvariation on 12SrRNA gene to investigate haplotype and nucleotide diversity, as well aspopulation structure of bats in the genus Aethalops. Samples consisted of 21 individualsfrom 7 populations of 5 islands (Sumatra, Jawa, Kalimantan, Bali and Lombok) coveringall islands distribution of this genus in Indonesia. Total DNA was extracted from livertissues. Fragment 12SrRNA gene region of the mitochondria1 DNA were amplified byPolymerase Chain Reaction usingprimers of L 1091 and H 1478. Nucleotide sequenceswere determined to investigate genetic variation of this genus. Results indicated thatfrom 294 bp nucleotide sequences, 33 polymorphic sites with 13 haplotypes were foundamong 7 populations.Key Words: Variation, Bat, Aethalops , Chiroptera, Indonesia, 12SrRNAmitochondrial.
Co-Authors Maharadatunkamsi Maharadatunkamsi, Maharadatunkamsi