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Rantau, Deritha Ellfy
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Biochemistry, Genetics & Molecular Biology

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VARIASI JUMLAH KROMOSOM TALAS BENTUL (COLOCASIA ESCULENTA (L.) SCHOTT) IN VITRO HASIL PERLAKUAN ORIZALIN Ermayanti, Tri Muji; Rantau, Deritha Ellfy; Wulansari, Aida; Martin, Andri Fadillah; Hafiizh, Erwin Al
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3765

Abstract

ABSTRACTChromosome number analysis is required after polyploid induction with oryzalin. Flowcytometry analysis is a simple and quick method to determine the ploidy level, however, chromosome number analysis is needed in order to confirm variation in the chromosome numbers which has occurred. The aim of the research was to investigate chromosome number variation of polyploid taro (Colocasia esculenta) after in vitro treatment with oryzalin. Nine treated-oryzalin clones and four taro cultivars, as control treatment, were used in this experiment. Ploidy level confirmation was done by flowcytometry analysis, meanwhile chromosome number calculation was performed by squashing method. Roots were isolated from  in vitro plantlets for squashing, leaves were isolated from the same plantlets were used for flowcytometry analysis. At least three plants consisted of 6-52 cells having good chromosome distributions were calculated for their chromosome numbers. The results showed that ploidy level of taro corresponded to the number of chromosomes. Flowcytometry analysis of diploid, triploid, tetraploid as well as hexaploid clones, all has chromosome numbers similar to those as their ploidy levels. Range of the chromosome numbers varied, with most of cells had around their normal chromosome numbers. From 5 to 15% of cells had aneuploid numbers lower or above their normal chromosome numbers.  Keywords : Colocasia esculenta, flowcytometer, polyploid, chromosome number, oryzalin, in vitro  
VARIASI JUMLAH KROMOSOM TALAS BENTUL (COLOCASIA ESCULENTA (L.) SCHOTT) IN VITRO HASIL PERLAKUAN ORIZALIN Ermayanti, Tri Muji; Rantau, Deritha Ellfy; Wulansari, Aida; Martin, Andri Fadillah; Hafiizh, Erwin Al
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3765

Abstract

ABSTRACTChromosome number analysis is required after polyploid induction with oryzalin. Flowcytometry analysis is a simple and quick method to determine the ploidy level, however, chromosome number analysis is needed in order to confirm variation in the chromosome numbers which has occurred. The aim of the research was to investigate chromosome number variation of polyploid taro (Colocasia esculenta) after in vitro treatment with oryzalin. Nine treated-oryzalin clones and four taro cultivars, as control treatment, were used in this experiment. Ploidy level confirmation was done by flowcytometry analysis, meanwhile chromosome number calculation was performed by squashing method. Roots were isolated from  in vitro plantlets for squashing, leaves were isolated from the same plantlets were used for flowcytometry analysis. At least three plants consisted of 6-52 cells having good chromosome distributions were calculated for their chromosome numbers. The results showed that ploidy level of taro corresponded to the number of chromosomes. Flowcytometry analysis of diploid, triploid, tetraploid as well as hexaploid clones, all has chromosome numbers similar to those as their ploidy levels. Range of the chromosome numbers varied, with most of cells had around their normal chromosome numbers. From 5 to 15% of cells had aneuploid numbers lower or above their normal chromosome numbers.  Keywords : Colocasia esculenta, flowcytometer, polyploid, chromosome number, oryzalin, in vitro  
ACCLIMATION AND AGRONOMIC PERFORMANCE OF POLYPLOIDS CLONES OF ARTEMISIA ANNUA L. Rahman, Wiguna; Hafiizh, Erwin Al; Ermayanti, Tri Muji; Rantau, Deritha Ellfy; Lelono, Arthur A.
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3092

Abstract

ABSTRACTSomatic cell manipulation of Artemisia annua L. was conducted by induction of polyploid plants with Colchicine and Oryzalin in order to increase level of artemisinin. Polyploid plantlets were multiplied on MS medium without plant growth regulators. After acclimation processes, plants were grown in the field for agronomic performance observation. Survival rate of plantlets was recorded. Agronomic performance of plants was observed by recording height of plants, number of branches, leaf biomass, stomatal characteristics, and artemisinin content. The results showed that survival rate of the plantlets from Colchicine and Oryzalin treatments were ranging from 13.40 to 33.33% and 11.11 to 41.67%, respectively. Growth rates of plant height and plant branching were not significantly different between diploid and tetraploid plant both from Colchicine and Oryzalin treatments, except to triploid plants from Colchicine treatment. Averages of plant height from Colchicine and Oryzalin treatments were ranging from 10.0 to 220.0 cm and from 35.0 to 186.0 cm, respectively. The averages number of branches per plant of polyploid plants from Colchicine and Oryzalin treatments were ranging from 3 to 66 and from 11 to 63, respectively. Averages of dry leaves biomass between diploid and tetraploid plant from Colchicine and Oryzalin treatments were also not significantly different. They were ranging from 12 to 64 g/plant and from 11 to 62 g/plant, respectively. However, tetraploid clones have bigger size of stomata and produced more artemisinin than the diploids.Keywords: Artemisia annua L, Colchicine, Oryzalin, Polyploids, Acclimation, Agronomic performance
Co-Authors Hafiizh, Erwin Al Lelono, Arthur A. Martin, Andri Fadillah Tri Muji Ermayanti Wiguna Rahman Wulansari, Aida