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Peningkatan Pengetahuan Murid SMAN 1 Grogol Terkait Protokol Kesehatan dan Cara Meningkatkan Imunitas Selama Masa Pandemi COVID-19 Putra, Edgar Alief Angkasa; Perdana, Sang Gede Farell Iswara; Elisia, Latiful; Faizah, Zakiyatul
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : CV. Ridwan Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.598 KB) | DOI: 10.36418/syntax-literate.v7i1.5715

Abstract

Penyebaran virus COVID-19 di Indonesia masih aktif di tahun 2021 disebabkan oleh kurang patuhnya masyarakat dalam menerapkan protokol kesehatan dan penyebaran berita palsu melalui sosial media yang membuat masyarakat enggan mematuhi protokol kesehatan dan meremehkan keadaan pandemi COVID-19. Angka terkonfirmasi COVID-19 di Indonesia mencapai 3.194.733 dan Provinsi Jawa Timur sendiri mencakup 8,8% dari kasus di seluruh Indonesia, dalam upaya mengurangi angka pasien terinfeksi dan mencegah bertambahnya pasien, kami ingin mengedukasi siswa-siswi SMAN 1 Grogol untuk meningkatkan pengetahuan terhadap bahaya COVID-19, fakta mengenai COVID-19, sosialisasi mengenai pentingnya protokol kesehatan, juga mengedukasi para pelajar agar tidak mudah mempercayai hoax yang sering beredar terutama di kalangan pelajar SMA yang aktif menggunakan sosial media. Kami harap dengan adanya proses edukasi ini, siswa-siswi dapat memiliki pemahaman lebih baik mengenai situasi pandemi di Indonesia, dapat menggunakan pengetahuan mereka untuk mencegah COVID-19 di kalangan sekolah dan keluarga. Metode penelitian yang digunakan dalam Kegiatan edukasi dilakukan dengan konsep webinar secara daring menggunakan aplikasi Zoom meeting yang dimana pada awal dan akhir kegiatan siswa diwajibkan mengisi pre-test dan post-test yang diberikan oleh panitia. Hasil yang didapati dalam penelitian ini adalah Sebanyak 180 responden telah mengerjakan pre-test dan post-test yang sesuai dengan kriteria inklusi dan eksklusi. Secara umum, tingkat pengetahuan dan pemahaman siswa-siswi kelas 10 SMAN 1 Grogol cukup baik. Nilai rata-rata pre-test adalah 82 sedangkan nilai rata-rata post-test adalah 88. Terdapat perbedaan signifikan (p < 0,05) tingkat pengetahuan dan pemahaman siswa (p = 0,000) sebelum dan sesudah kegiatan pengabdian masyarakat ini. Kesimpulan yang di dapat perbedaan signifikan pada tingkat pengetahuan dan pemahaman siswa sebelum dan sesudah kegiatan pengabdian masyarakat ini.
Hyaluronan Expression on Vitrified Oocytes Before and After In Vitro Maturation (EKSPRESI HYALURONAN PADA OOSIT YANG DIVITRIFIKASI SEBELUM DAN SESUDAH MATURASI IN VITRO) Zakiyatul Faizah; R. Haryanto Aswin; Hamdani Lunardhi; Widjiati Widjiati
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.094 KB) | DOI: 10.19087/jveteriner.2018.19.1.71

Abstract

Oocyte vitrification is a major challenge in assisted reproductive technology. Oocyte vitrification with cumulus cells provide benefits in the process of maturation and fertilization. Vitrification leads to rapid temperature changes, therefore the decreasing in temperature could damage the cells even when the morphology was normal. Vitrification of mature oocytes is common because of its low sensitiveness towards low temperatures than immature oocytes. The aim of the research was to compare the effect of vitrification before and after in vitro maturation to the expression of hyaluronan. Maturation was operated in medium TC 50 ?L in CO2 incubators for 24 hours. Vitrification started with washing oocyte in PBS basic medium supplemented with 20% serum for 1-2 minutes, then in equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing was processed by submerging the oocytes in the media: 1). PBS + 20% serum + 0.5 M sucrose (K1); 2) PBS + 20% serum + 0.25 M sucrose (K2); and 3).PBS + 20% serum + 0.1 M sucrose (K3). Immunocytochemical stain was performed to evaluate the hyaluronan expression. Remmele scale index (Immunoreactive score, IRS) was used to read the result. There was no differences of hyaluronan expression in oocyte and cumulus group of K1, K2 and K3 at p< 0.05, statistically. We concluded that there was no difference of hyaluronan expression on oocyte and cumulus between vitrified oocyte of pre and post in vitro maturation which indicated that oocyte could be vitrified in the immature and mature state.
Ekspresi Transforming Growth Factor-beta dan Growth Differentiation Factor-9 Oosit Domba yang Divitrifikasi Sesudah dan Sebelum Maturasi In Vitro Zakiyatul Faizah; Raden Haryanto Aswin
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.212 KB) | DOI: 10.19087/jveteriner.2021.22.1.109

Abstract

Oocyte vitrification today became a hope to preserve fertility. Its was a major challenge because of oocyte characteristic in every phase. Immature oocytes were more sensitive to osmotic stress and the membrane wes less stable while mature oocyte have spindles that were very susceptible to temperature decrease. The study aim to compare the effect of vitrification before and after in vitro maturation to the expression TGF beta and GDF9. Oocyte of ewes divided into control groups (K0), K1 maturation prior vitrification, K2 vitrification prior maturation. Vitrification begins with washing oocytes in PBS supplemented of 20%serum for 1-2 minutes, followed by equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing was processed by in the media: 1). PBS + 20% serum + 0.5 M sucrose, 2).PBS + 20% serum + 0.25 M sucrose, and 3).PBS + 20% serum + 0.1 M sucrose. Immunocytochemical stain was performed to evaluate TGF ? and GDF9 expression. Remmele scale index (IRS) was used to read the result. TGF beta expression both in oocyte and cummulus of K0 and K1 was significant statistically difference (p<0.05) compare with K2. GDF9 expression both in oocyte and cummulus of K0 and K1 was significant statistically difference (p<0.05) compare with K2. We concluded that immature oocyte give better expression of TGF â and GDF9 than mature oocyte.
Effect of oocyte vitrification before and after in vitro maturation towards Bcl-2, Bax and Bcl-2/Bax ratio expression Zakiyatul Faizah; R. Haryanto Aswin; Hamdani Lunardhi
Majalah Obstetri dan Ginekologi Vol. 24 No. 2 (2016): May - August
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.057 KB) | DOI: 10.20473/mog.V24I22016.56-60

Abstract

Objectives: to compare the expression of Bcl-2, Bax and Bcl-2/Bax ratio in cumulus cell and oocyte between vitrified oocyte pre and post in vitro maturation.Materials and Methods: Maturation was operated in medium TC 100 µl for 24 hours. Vitrification begins with washing oocyte in PBS basic medium supplemented of 20% serum for 1-2 minutes, followed by equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing is processed by submerging the oocytes in the media: 1). PBS + 20% serum + 0.5 M sucrose, 2). PBS + 20% serum + 0.25 M sucrose, and 3). PBS + 20% serum + 0.1 M sucrose. Imunocytochemistry observed the expression of Bcl-2, bax and Bcl-2/bax ratio.Results: Bcl-2 expression on oocyte in control group differed significantly with treatment group, Bcl-2 expression on cumulus in control group differed significantly with treatment 1 group. Bax expression on oocyte in control group differed significantly with treatment group. Bax expression on cumulus in control group differed significantly with treatment group. Bcl-2/Bax expression ratio on oocyte and cumulus did not differ significantly in all groupConclusion: No difference Bcl-2/Bax expression ratio on oocyte and cumulus between vitrified oocyte pre and post in vitro maturation.
Comparison of pregnancy rates on day 3 and day 5 embryo transfer in In Vitro Fertilization (IVF) Audia Mumtaz Rifasky; Puspa Wardhani; Ashon Sa’adi; Ninik Darsini; Hamdani Lunardhi; Zakiyatul Faizah
Majalah Obstetri dan Ginekologi Vol. 29 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V29I12021.14-17

Abstract

Objectives: To identify the success rates of pregnancy on the third and fifth day embryo transfer at Graha Amerta Hospital, Surabaya, Indonesia.Materials and Methods: This study used comparative cross sectional design. Data were taken from medical record of IVF participants who met the inclusion and exclusion criteria at Graha Amerta Hospital for the period of January 2016 - December 2016.Results: Successful pregnancy rates were found in this research. The embryo transfer on the third day and the fifth day were 35% and 49.3% respectively. In other words, the rates of pregnancy success were not affected by embryo transfer on the third day and the fifth day in the medical record sample as it had p value of 0.090.Conclusion: Embryo transfer on the third and fifth days had the same rates of pregnancy success in IVF participants at Graha Amerta Hospital, Surabaya, Indonesia. 
FERTILIZATION OF BOVINE OOCYTES VITRIFIED PRE- AND POST IN VITRO MATURATION Zakiyatul Faizah; Ninik Darsini; Aucky Hinting
Folia Medica Indonesiana Vol. 52 No. 2 (2016): APRIL - JUNE 2016
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.526 KB) | DOI: 10.20473/fmi.v52i2.5222

Abstract

The success rate of fertilization post save frozen oocytes is still very low, because the oocyte has distinctive features, namely the volume ratio and a lower surface to the limited penetration of water and cryoprotectants penetrate cells. Beside mature oocytes have a thread spindles are particularly vulnerable to the drop in temperature. Keep frozen oocytes is needed, especially in women who needed rescue fertility so their oosit can be fertilized. Maturation is done in TC 100 mL medium covered with mineral oil in a petri dish with a diameter of 36 mm. Oocyte vitrification begins with washing in PBS supplemented medium serum 20% for 1-2 minutes, followed by serum in the medium PBS + 20% + 10% ethylene glycol for 10-14 minutes. Then oocyte vitrification medium is transported in PBS + serum 20% + sucrose 0.5M ethylene glycol + 15% + 15% PROH for 25-30 seconds. Thawing oocytes is done by successive immersed in the media: 1). PBS + 20% serum + 0.5M sucrose, 2). PBS + 20% serum + 0.25M sucrose, and 3). PBS + 20% serum + 0.1 M sucrose. Insemination is done in rosset, and the number of fertilization was observed after 48 hours. Fertilization in the control group amounted to 42.97%, while the K1 and K2 there are no fertilization at all. The analysis showed that fertilization in the control and treatment groups significantly different at p <0.05 in both treatment groups K1 or K2 there are no fertilization at all. The conclusions of this study is there is no difference between the amount of fertilization of bovine oocytes were vitrified pre and post-maturation in vitro.
PEMBAGIAN PAKET SEMBAKO SEBAGAI BENTUK BANTUAN SOSIAL KEPADA PEKERJA JALANAN DI WILAYAH KELURAHAN CILENDEK TIMUR DALAM MASA TRANSISI PANDEMIK COVID-19 Hanifah Puspita Nurul H; Anggi Senda Nikenindya; Dhien Meutia Islamadina Wulandani; Permata Aprilia Putri; Cahya Ramadhanti Putri; Zalfa Qanita; Almira Nadya Yasmine; Saskia Ramadhanti; Rangga Panengah B; Zakiyatul Faizah
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 4 No. 2 (2020): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v4i2.2020.486-491

Abstract

COVID-19 is a zoonotic disease that has the ability to transmit from animals to humans and transmission occurs through droplets, therefore the government appeal on people to carry out social distancing and quarantine. The purpose of this activity is to help food needs and facilitate the people who affected by COVID - 19 to be able to apply government program is Pola Hidup Bersih dan Sehat (PHBS) independently as an effort to prevent an increase in COVID - 19 cases. The implementation method is community service, starting with channeling social assistance in the form of distribution of groceries, hand sanitizers and liquid soap to the community around the streets of the City of Cilendek Timur, Bogor City. The results of this activity are a form of community service activities that are focused on one of the fields, namely the economic field, where the activities carried out in the form of providing social assistance by distributing nine basic goods or food to people in need.abstrakCOVID-19 merupakan penyakit zoonosis yang memiliki kemampuan menularkan dari hewan kepada manusia dan penularan terjadi melalui droplets, maka dari itu pemerintah menghimbau masyarakat untuk melakukan social distancing dan karantina. Tujuan dari kegiatan ini adalah membantu mencukupi kebutuhan pangan dan memfasilitasi masyarakat yang terdampak COVID – 19 untuk dapat menerapkan Perilaku Hidup Bersih dan Sehat (PHBS) secara mandiri sebagai upaya mencegah peningkatan kasus COVID – 19. Metode pelaksanaan adalah pengabdian masyarakat, dimulai dengan menyalurkan bantuan sosial dalam bentuk pembagian sembako, hand sanitizer dan sabun cair kepada masyarakat disekitar jalan Kelurahan Cilendek Timur Kota Bogor. Hasil dari kegiatan ini merupakan bentuk dari kegiatan pengabdian masyarakat yang difokuskan pada salah satu bidang yaitu bidang ekonomi, dimana kegiatan yang dilakukan diwujudkan dalam bentuk pemberian bantuan sosial dengan cara membagikan sembilan bahan pokok atau sembako kepada orang-orang yang membutuhkan.
NATURAL DEAF EDUCATION FOR THE COMMUNITY Puguh Setyo Nugroho; Agustinus Agustinus; William William; Eko Budi Siswidiyanto; Nur Rohmah; Zakiyatul Faizah
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 6 No. 1 (2022): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v6i1.2022.69-76

Abstract

Congenital deafness will impact the quality of life of affected individuals if they do not obtain early detection and intervention. Socialization of congenital deafness is needed, thereby the community can engage in early detection and intervention efforts. Socializing about the early detection and intervention of congenital deafness. Educational activities are carried out with counseling using the lecture method, discussion, and pretest and posttest. The average pretest result was 62.03 (± 11.51) and the posttest average was 88.39 (± 13.41). Based on the comparison between the pretest and posttest results, we found a significant difference (p < 0.0001) statistically. Public knowledge of congenital deafness needs to be improved in order to increase community participation in terms of  early detection and intervention.
Pengaruh Seminar Online terhadap Peningkatan Pengetahuan Murid SMAN 1 Purwoasri Terkait COVID-19 pada Anak dan Remaja Selama Pandemi COVID-19 Muhammad Ircham Gustriawan; Muhammad Fadhil Kamaruddin; Pandya Daniswara Winantyo; Zakiyatul Faizah
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : CV. Ridwan Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.598 KB) | DOI: 10.36418/syntax-literate.v7i1.5740

Abstract

Permasalahan di bidang kesehatan yang belakangan ini mendapat perhatian oleh pemerintah yaitu mengenai COVID-19. Pada tanggal 11 Maret 2020, WHO menetapkan COVID-19 sebagai pandemi sehingga mengharuskan pembelajaran dilaksanakan secara online. Dengan meningkatnya kasus COVID-19 di Indonesia, salah satu sektor yang sangat terdampak yaitu pendidikan. Tujuan utama dari penelitian ini adalah untuk meningkatkan pengetahuan dan pemahaman siswa SMA tentang COVID-19 pada anak dan remaja, serta meningkatkan kesiapan siswa-siswi dalam pembelajaran luring dan daring di era pandemi ini. Penelitian ini dilaksanakan secara daring melalui zoom dengan peserta webinar sebanyak 262 orang. Evaluasi diperoleh dari penilaian pre-test dan post- test melalui angket online yang berisi 20 pertanyaan mengenai materi COVID-19 pada anak dan remaja, evaluasi dilakukan dengan menilai peningkatan pengetahuan peserta melalui perbandingan nilai antara pre-test dan post- test, kemudian dilakukan uji statistik Wilcoxon Signed Ranks untuk mengetahui apakah terdapat perbedaan yang signifikan antara nilai pre-test dan post-test. Hasil dari pre-test dan post-test didapatkan peningkatan nilai rata-rata sebanyak 3,7% setelah dilaksanakan webinar. Didapatkan nilai rata-rata pre test responden 87% dan rerata post test sebesar 90,7%. Terdapat peningkatan pengetahuan dan pemahaman siswa terhadap pencegahan penularan virus Covid-19 setelah menghadiri seminar online.
Faktor Sosial Budaya yang Memengaruhi Kesehatan Ibu dan Anak (Studi Etnografi) Salsabila Salsabila; Zakiyatul Faizah; Budi Prasetyo
Oksitosin : Jurnal Ilmiah Kebidanan Vol. 9 No. 1 (2022): OKSITOSIN : Jurnal Ilmiah Kebidanan
Publisher : Prodi D III Kebidanan Fakultas Ilmu Kesehatan Universitas Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35316/oksitosin.v9i1.1651

Abstract

Maternal and child health as a key indicator of community welfare is measured by Maternal Mortality Rate (MMR) and Infant Mortality Rate (IMR). The problem of maternal and child mortality is a problem that cannot be separated from the culture or behavior of the community itself. The social and cultural system in the community is an important consideration in health services that can be utilized more optimally. The purpose of this study was to determine what cultural factors affect the health of mothers and children. This research is a rapid ethnographic assessment procedure (REAP) with a qualitative approach. Sampling technique using snowball or chain referral sampling. It is carried out sequentially from one participant to another. Is a variation of the purposive sample. The number of respondents as many as 8 people who fit the inclusion criteria. The data shown were the result of indepth interviews and FGDs (Focus Group Discussion). This study resulted in findings about: (1) Kyai become community role models in responding to life's problems ranging from political choices to choices of access to health. (2) Women do not have sufficient full power or empower themselves to choose health services, especially during pregnancy and childbirth. (3) The element of belief inherited from generation to generation also forms the knowledge system of the Cibitung community. (4) The livelihood system of farm laborers as the main source of the economy in Cibitung. Keywords: Socio-Cultural, Ethnographic, Religious Systems, Kinship Systems, Knowledge Systems, Livelihood Systems