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IRADIASI SINAR ? PADA BIAK TUNAS KENTANG HITAM (SOLANOSTEMON ROTUNDIFOLIUS) EFEKTIF UNTUK MENGHASILKAN MUTAN Witjaksono, Witjaksono; Leksonowati, Aryani
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3075

Abstract

Irradiation of ??ray at shoot culture of Kentang hitam (Solanostemon rotundifolius) is effective for mutant production. Kentang hitam is sterile and vegetatively propagated andtherefore its genetic diversity is narrow. Mutation is an alternative way to increase genetic heterogeneity. Irradiation of shoot cultures with different doses followed by culturing of the inoculum (leaf, petiole and internodes) from that irradiated culture on a regeneration medium MS containing 5 mg/l BA and 0.1 mg/l NAA has resulted in curvilinear respon of growth, morphogenesis and plant regeneration. The growth variables increase from 0 to 6 Gy and thendecrease to 25 and plateu or increase a little at 35 Gy and growth death wes observed at 50 Gy. Leaf and petiole inocula were more responsive than the internode. Respon of growth of shootregeneration of 50% were obtained at doses of 10-12.5 Gy. However higher level of doses, such as 25 Gy had also been effective for inducing mutant. Morphological and growth different were observed from growth in tissue culture to the field. Mutants were recovered, forexample, the one with early flowering.Keywords : Solanostemon rotundifolius, Irradiation, gammarays, cultur in vitro, mutan
Moringa oleifera Distribution in Java and Lesser Sunda Islands which is Attributed with Annual Rainfall Riastiwi, Indira; Damayanto, I Putu Gede P.; Ridwan, Ridwan; Handayani, Tri; Leksonowati, Aryani
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.16115

Abstract

Moringa (Moringa oleifera Lam.) is a cultivated plant that reported have many benefits. Indonesian people have utilized moringa as a vegetable widely but it is never used as functional food. The distribution data of moringa in Indonesia is needed to develop moringa as an additional nutrient for functional food. The aim of the research was to draw up the distribution map of moringa in Java and Lesser Sunda Islands (LSI). In addition, this distribution map was also attributed with average annual rainfall to know the preference of moringa to life depend on the rainfall. This research has been conducted with four approaches: collecting specimens, direct observation of living plant, direct observation of specimen herbarium and literature study. All data were analyzed and arranged into the distribution maps of moringa in Java and the LSI attributed with annual rainfall. The result showed that moringa in entire Java mostly distributed in the area of Jakarta, West Java, Central Java, Yogyakarta, the north part of East Java, Madura and Kangean. While, moringa in LSI distributed in the southern part of Bali, Lombok, Sumbawa, Kupang, Flores, Sumba and Alor. The average annual rainfall of 1500-2000 mm is the most ideal condition for moringa plants to grow well. This map and information can be utilized by the stakeholders to determine the most appropriate place for moringa cultivation and their development as as a functional food.