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Uji aktivitas antibakteri ekstrak etanolik umbi mentimun papasan (Coccinia grandis L.Voigt) terhadap Shigella dysenteriae dan Staphylococcus aureus Rukmana, Rizal Maarif; Nugroho, Rahmat Budi; Wisnumurti, Dwi Admani; Wibawa, Andang Arif
Riset Informasi Kesehatan Vol 8 No 2 (2019): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.24 KB) | DOI: 10.30644/rik.v8i2.233

Abstract

Abstrak Latar Belakang: Penyakit infeksi merupakan salah satu penyakit yang menjadi masalah serius di negara berkembang khususnya di Indonesia. Bakteri yang sering menimbulkan penyakit infeksi adalah bakteri Shigella dysentriae dan Staphylococcus aureus. Metode: Serbuk umbi Mentimun Papasan (Coccinia grandis L.Voight) dilakukan ekstraksi dengan metode maserasi. Pelarut yang dipakai adalah etanol 96%. Identifikasi golongan senyawa dilakukan dengan menggunakan pereaksi kimia. Uji aktivitas antibakteri dilakukan dengan metode difusi sumuran. Hasil: Hasil identifikasi golongan senyawa menunjukkan adanya golongan senyawa flavonoid, tanin, saponin, alkaloid dan polifenol. Hasil uji antibakteri ekstrak umbi Mentimun Papasan kosentrasi 1 gr/ml, 2 gr/ml, 3 gr/ml dan 4 gr/ml terhadap bakteri Shigella dysentriae berturut-turut: 18mm, 19,66 mm, 21,23 mm dan 23,33 mm. Hasil uji antibakteri ekstrak umbi Mentimun Papasan kosentrasi 1 gr/ml, 2 gr/ml, 3 gr/ml dan 4 gr/ml terhadap bakteri Staphylococcus aureus berturut-turut: 18,67 mm, 20,67 mm, 23,33 mm dan 27 mm. Kesimpulan: Ekstrak etanolik umbi Mentimun Papasan dapat menghambat bakteri Shigella dysentriae dan Staphylococcus aureus.
UJI AKTIVITAS ANTI BAKTERI EKSTRAK ETANOLIK UMBI AKAR BATU (Coccinia grandis L.Voight) TERHADAP BAKTERI SALMONELLA SP Rukmana, Rizal Maarif; Nugroho, Rahmat Budi; Wisnumurti, Dwi Admani
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 2: September 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i2.8390

Abstract

AbstractSalmonella sp are bacteria that often infect human and animal. This type of bacteria can cause Salmonellosis in human. Previous research has been carried out on secondary metabolites of plants as medicinal ingredients. One of the plants that has potential as a medicine is stone Akar Batu (Coccinia grandis L Voight). This study aims at examining the antibacterial activities of ethanolic extract of stone Akar Batu on Salmonella sp. The extraction method was carried out by maceration and the solvent used was ethanol 96%. Antibacterial activity was conducted using diffusion method. Identification of compound groups of extracts was done by reacting extracts with various chemical reagents. The results showed that stone of Akar Batu powder had a moisture content of 5.99% and a yield of 1.76%. The identification results of ethanolic extract of stone Akar Batu chemical group indicated that the extract contained flavonoids, alkaloids, saponins and tannins. The results of antibacterial activity test have revealed that ethanolic extract of stone Akar Batu can inhibit Salmonella sp.