Maxs Urias Ebenhaizar Sanam, Maxs Urias
Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner Fakultas Kedokteran Hewan, Universitas Nusa Cendana Jl. Adisucipto, Penfui, Kupang, Nusa Tenggara Timur, Indonesia 85001

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SDR-2 as a strong candidate vaccine for brucellosis in animals Sanam, Maxs U.E
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 3 (2001)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.849 KB) | DOI: 10.14334/jitv.v6i3.236

Abstract

Various mutant strains of Brucella suis type-1 have been recently developed including SDR-2 and SD-7. This research was aimed at revealing the course of infection and serological reactions, as well as the protection capacity of these mutant strainscompared to the reference vaccine strain 19, and the virulent strain B. suis type-1 in Quackenbush mice as a model. Antibody reactions were measured by an Enzyme-linked Immunosorbent Assay (ELISA) and degree of infection was determined by bacterial spleen count. The results showed that the SD-7 was unable to perform infection in mice. Whereas SDR-2, strain 19 and the virulent B. suis type-1 were able to colonize the mice spleens with varying rate of infections. The inoculation of SDR-2 to the mice produced mild infection and lasted shorter than the virulent strain even with the reference vaccine strain 19. The number of SDR-2 and strain 19 organisms were sharply dropped at week-12 post inoculation while that of virulent strain was significantly remained high. Serological responses induced by SDR-2 was the lowest followed by those of strain 19, and the virulent strain. On the challenge with a virulent B. suis, histological examinations of the spleen of the control mice revealed that there was a marked depletion of lymphoid cells and reticuloendothelial hyperplasia in lymphoid follicles. However no significant pathological changes were observed in groups inoculated with either SDR-2 or Strain 19. Enumeration of survival challenge organisms in the spleens clearly demonstrated that SDR-2 provided significant protection (2.17 Log10) to the animals which was comparable to that provided by strain-19 (2.20 Log10). In conclusion, SDR-2 has a potential as a vaccine for use in pigs against Brucella suis infection. Furthermore SDR-2 offers some advantages over strain 19 in that it is less virulent and induces less antibody responses than the strain 19 and thus may have application in other animals. However, furher study on its efficacy as a vaccine for brucellosis in pigs as the primary host for B. suis needs to be assessed.   Key words: Brucella suis, mutant strains, SDR-2 vaccine, strain 19, antibody
SDR-2 as a strong candidate vaccine for brucellosis in animals Maxs U.E Sanam
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 3 (2001): SEPTEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.849 KB) | DOI: 10.14334/jitv.v6i3.236

Abstract

Various mutant strains of Brucella suis type-1 have been recently developed including SDR-2 and SD-7. This research was aimed at revealing the course of infection and serological reactions, as well as the protection capacity of these mutant strainscompared to the reference vaccine strain 19, and the virulent strain B. suis type-1 in Quackenbush mice as a model. Antibody reactions were measured by an Enzyme-linked Immunosorbent Assay (ELISA) and degree of infection was determined by bacterial spleen count. The results showed that the SD-7 was unable to perform infection in mice. Whereas SDR-2, strain 19 and the virulent B. suis type-1 were able to colonize the mice spleens with varying rate of infections. The inoculation of SDR-2 to the mice produced mild infection and lasted shorter than the virulent strain even with the reference vaccine strain 19. The number of SDR-2 and strain 19 organisms were sharply dropped at week-12 post inoculation while that of virulent strain was significantly remained high. Serological responses induced by SDR-2 was the lowest followed by those of strain 19, and the virulent strain. On the challenge with a virulent B. suis, histological examinations of the spleen of the control mice revealed that there was a marked depletion of lymphoid cells and reticuloendothelial hyperplasia in lymphoid follicles. However no significant pathological changes were observed in groups inoculated with either SDR-2 or Strain 19. Enumeration of survival challenge organisms in the spleens clearly demonstrated that SDR-2 provided significant protection (2.17 Log10) to the animals which was comparable to that provided by strain-19 (2.20 Log10). In conclusion, SDR-2 has a potential as a vaccine for use in pigs against Brucella suis infection. Furthermore SDR-2 offers some advantages over strain 19 in that it is less virulent and induces less antibody responses than the strain 19 and thus may have application in other animals. However, furher study on its efficacy as a vaccine for brucellosis in pigs as the primary host for B. suis needs to be assessed.   Key words: Brucella suis, mutant strains, SDR-2 vaccine, strain 19, antibody
Kerugian Ekonomi Akibat Penyakit Rabies di Provinsi Nusa Tenggara Timur (ECONOMICAL LOSSES OF RABIES DISEASE IN EAST NUSA TENGGARA PROVINCE) Ewaldus Wera; Maria Geong; Maxs Urias Ebenhaizar Sanam
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.248 KB)

Abstract

The objective of this paper was to analyze economic impact of rabies in East Nusa Tenggara Province.Data from Health Department of East Nusa Tenggara Province (Period 1998-2007) were applied to a setof link the economics model. Analysis presented in this paper only costs related with PET, Vaccination andelimination of dogs. The total societal cost (PET in human) incurred by the disease was about Rp 19.9billion. The cost included transport cost to and from rabies-treatment centers, and loss of income whilereceiving treatment. The cost of vaccination was estimate about Rp 50.000 per dog and about 140.000dogs were vaccinated per year in the area. For this analysis, vaccination costs per dog included relatedcomponents on campaign organization, public awareness efforts, transport and biological and materialcosts. The economic losses due to the culling and vaccination program in dogs wes about Rp 5,3 and Rp 7billion per year, respectively. Total cost for vaccination and elimination of dog from 1998 through 2007 wasabout Rp 122,5 billion or Rp 12,3 billion per year. Total economic lost due to rabies in East Nusa TenggaraProvince during 1998-2007 was approximately 14,2 per year. The economic losses may be reduced byoptimalisation of rabies control in animal reservoir of rabies especially in dogs. The elimination of rabiesin dogs through mass vaccination with minimal coverage 70% of dog population is believed contribute tominimise rabies in human.
Keragaman Endoparasit pada Macaca fascicularis dan Potensi Zoonotiknya dengan Cuaca Berbeda di Kota Kupang (ENDOPARASITIC DIVERSITY IN MACACA FASCICULARIS AND ITS ZOONOTIC POTENTIAL ON DIFFERENT WEATHER IN KUPANG CITY) Jayusman Arsiyanti Joesoef; Dondin Sajuthi; Agus Wijaya; Maxs Urias Ebenhaizar Sanam
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.564 KB) | DOI: 10.19087/jveteriner.2018.19.4.451

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Long-tailed macaque (Macaca fascicularis) belongs to least concern categories of animals, and often found in various regions of Indonesia, including Kupang, East Nusa Tenggara. Their habitat are one of the tourist destinations, causing interaction between them and humans. The interaction facilitates the transmission of disease agents, for example endoparasit. This study was conducted to find out the diversity and prevalence of endoparasites in long-tailed macaque, weather effects, and zoonotic potential in Kupang City. Fifty fecal samples were collected and analyzed using formalin ethyl acetate concentration technique (FECT). A total of six endoparasites were recovered including four helmints (hookworm, Strongyloides, Toxocara, and Acantocephala) and two protozoans (Balantidium coli and Entamoeba sp.). The highest prevalence of infection occurred in February (rainfall 302.4 mm) and consisted of hookworm (86%) and B. coli (66%). If rainfall increases, prevalence will also increase. The high prevalence of parasites is not always associated with the appearance of clinical symptoms. An individu diagnosed with parasites if the eggs or cysts have been identified from the faeces. The high prevalence caused by the absence of deworming program. Among the endoparasites that have been found, some of which are potentially zoonotic, such as: Strongyloides sp., Toxocara sp., Acanthocephala, B. coli, and Entamoeba sp.
Analisis Genetik Gen Protective Antigenic pada Bacillus anthracis Isolat Jawa Tengah dan Yogyakarta (GENETIC ANALYSIS ON PROTECTIVE ANTIGENIC GENE OF BACILLUS ANTHRACIS ISOLATES OF CENTRAL JAVA AND YOGYAKARTA) Maxs Urias Ebenhaizar Sanam; Widya Asmara; Agnesia Endang Tri Hastuti Wahyuni; Michael Haryadi Wibowo
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to determine sequence and genotype diversity of protective antigenic gene ofBacillus anthracis isolated from Central Java and Yogyakarta. Pag-A gene which encodes for antigenicprotein is one toxin component and the virulent factor of B. anthracis. As many as five isolates fromSemarang, Sragen, and Boyolali (Central Java) and Sleman (Yogyakarta) were used. The gene wassequenced and amplified used three set of primers PA1857/PA2436, PA8/PA5, and PA-5F/PA-5R. Theresult showed that the nucleotide sequences of gene from five isolates were identical and only had onenucleotide difference as compared to B. anthracis sterne M22589. All isolates were confirmed as genotypebased on pag-A sequence. It was concluded that all B. anthracis from Central Java and Yogyakarta haveidentical pag-A sequence and belong to genotypt-1. Further studies are needed to investigate B. anthracisisolates from other regions of Indonesia.
PENDAMPINGAN PENGOLAHAN LIMBAH PETERNAKAN SAPI POTONG DI KELOMPOK TANI TERNAK UNTUK MENDUKUNG PERTANIAN SKALA RUMAH TANGGA, DESA CAMPLONG II, KABUPATEN KUPANG, NTT Cynthia Gaina; Frans Umbu Datta; Maxs U.E Sanam; Filphin Adolfin Amalo; Imanuel Benu; Meity Marviana Laut
Jurnal Pengabdian Masyarakat Peternakan Vol 5, No 1 (2020): Jurnal Pengabdian Masyarakat Peternakan
Publisher : Jurusan Peternakan Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.401 KB) | DOI: 10.35726/jpmp.v5i1.418

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Kegiatan pengabdian ini bertujuan untuk meningkatkan pemahaman masyarakat petani peternak dalam memanfaatkan limbah peternakan menjadi pupuk kandang organik untuk menunjang aktivitas pertanian di desa Camplong II, kabupaten Kupang, NTT. Hasil samping berupa limbah feses telah diolah menjadi pupuk organik yang dimanfaatkan dalam lahan pertanian rumah tangga kelompok tani. Secara umum, sistem kepemilikan sapi di kelompok ternak ini adalah setiap anggota bertanggung jawab untuk merawat ternak sapinya masing-masing sehingga limbah yang diperoleh dimanfaatkan lagi oleh petani peternak tersebut. Kegiatan ini mendapat respon yang baik dari anggota kelompok yang juga memanfaatkan pupuk ini untuk kegiatan bertani di sekitar rumah masing-masing. Waktu pelaksanaan pendampingan adalah siang hari setelah anggota kelompok selesai memberi pakan ternak sapinya. Adapun kendala yang dihadapi oleh petani peternak desa Camplong II berupa kurangnya pengetahuan tentang manfaat limbah hasil peternakan yang dapat dimanfaatkan untuk menutrisi tanaman pertanian. Dimana hasil limbah ini hanya ditumpuk dan tidak dimanfaatkan. Melihat kondisi ini, maka diperlukan upaya untuk meningkatkan pengetahuan masyarakat melalui pendampingan pembuatan pupuk kompos yang bermanfaat bagi masyarakat petani peternak. Metode yang digunakan dalam kegiatan pengabdian ini meliputi pendampingan dan pemantauan pembuatan pupuk kompos bokashi. Kesimpulan yang diperoleh adalah peningkatan pengetahuan dan keterampilan peternak dalam mengelola hasil limbah peternakan untuk dimanfaatkan sebagai sumber nutrisi tanah pertanian skala rumah tangga di desa Camplong II.
PELATIHAN DAN PENDAMPINGAN BETERNAK AYAM KAMPUNG SUPER UNTUK PENINGKATAN EKONOMI DAN PERBAIKAN GIZI MASYARAKAT DI DESA CAMPLONG II Tri Utami; Maxs Urias Ebenheizer Sanam; Dewi F.L Djungu; Yeremia Yobelanno Sitompul; Tarsisius Considus Tophianong
Jurnal Pengabdian Masyarakat Peternakan Vol 5, No 1 (2020): Jurnal Pengabdian Masyarakat Peternakan
Publisher : Jurusan Peternakan Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (542.509 KB) | DOI: 10.35726/jpmp.v5i1.419

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Program pengabdian kepada masyarakat ini bertujuan untuk memberikan edukasi dan melatih masyarakat agar terampil dalam pengelolaan usaha peternakan ayam kampung.  Mitra kegiatan ini adalah sepuluh orang ibu-ibu yang memiliki profesi sebagai penenun (kain tenun Timor) dalam sanggar WOKOLEUTUAN di Dusun Dua Oelkiub, Desa Camplong II, Kabupaten Kupang, Provinsi Nusa Tenggara Timur. Beberapa tahapan pelaksanaan kegiatan pengabdian berbasis pemberdayaan masyarakat  ini, meliputi: (1) tahap pendahuluan berupa observasi lokasi kegiatan dan perumusan masalah yang dihadapi mitra, serta persiapan bahan material; (2) tahap berikutnya adalah penentuan metode pendekatan pemecahan masalah, melalui pelaksanaan kegiatan sosialisasi serta pelatihan beternak ayam yang meliputi penentuan lokasi kandang, penyiapan kandang dan peralatan, pembuatan pakan ransum, dan pemberian vaksinasi. Selain memberikan pelatihan, tim pelaksana juga memberikan bantuan kepada mitra berupa ayam kampung, obat-obatan, bahan untuk pembuatan kandang dan seperangkat peralatan penunjang untuk peternakan; (3) tahap akhir dari kegiatan ini meliputi monitoring, evaluasi dan pembuatan laporan akhir kegiatan. Melalui kegiatan sosialisasi, pelatihan beternak ayam kampung dan pendampingan yang telah dilakukan oleh tim pelaksana secara berkelanjutan diharapkan memberikan manfaat terhadap peningkatan pengetahuan dan keterampilan peternak ayam kampung super. Luaran yang dihasilkan dari kegiatan ini adalah terciptanya usaha peternakan ayam kampung skala rumah tangga sebagai salah satu solusi dalam pemenuhan kebutuhan gizi pangan maupun peningkatan perekonomian keluarga mitra. Kata Kunci: pelatihan, peternakan, ayam kampung
Pemanfaatan Limbah Organik sebagai Bahan Dasar Pembuatan Pupuk Organik Cair Pertanian di Desa Camplong II, Kec. Fatuleu, Kab. Kupang, NTT Cynthia Dewi Gaina; Frans Umbu Datta; Maxs U.E Sanam; Filphin Adolfin Amalo
Jurnal Pengabdian Masyarakat Peternakan Vol 5, No 2 (2020): Jurnal Pengabdian Masyarakat Peternakan
Publisher : Jurusan Peternakan Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.862 KB) | DOI: 10.35726/jpmp.v5i2.502

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Sebagian besar masyarakat Desa Camplong II, Kec. Fatuleu, Kab. Kupang memiliki mata pencaharian sebagai petani dan peternak. Desa ini memiliki topografi landai, bergelombang dengan kondisi tanah berkapur yang dikelilingi berbagai macam tumbuhan yang menyebabkan Desa Camplong II memiliki sampah organik lebih banyak dibandingkan sampah anorganik. Akan tetapi, selama ini sampah belum diolah secara optimal sehingga tumpukan sampah yang sebenarnya bermanfaat bagi pertumbuhan tanaman lainnya dibiarkan begitu saja. Oleh karena itu, melalui program pengabdian ini, tim pengabdi mengajak masyarakat desa untuk mengolah sampah organik menjadi produk yang bermanfaat bagi pertanian dengan membuat pupuk organik cair (POC). Pupuk organik cair merupakan larutan yang dihasilkan dari pembusukan alami bahan organik yang memiliki unsur hara yang dapat menyuburkan dan menggemburkan  lapisan tanah, dimana dapat membantu menyuburkan kondisi tanah di desa Campong II yang cenderung tidak dalam, mudah tererosi dengan drainase kurang baik serta mudah merekah pada musim panas. Metode kegiatan pengabdian ini berupa pelatihan, pendampingan dan praktik langsung pembuatan POC dari limbah organik pertanian baik yang berada di sekitar pekarangan rumah maupun yang berada di lokasi penanaman hijauan makanan ternak. Hasil yang dicapai berupa peningkatan pemahaman masyarakat dalam mengklasifikasi jenis dan manfaat sampah organik serta anorganik dan perubahan perilaku masyarakat dalam memanfaatkan limbah organik dari hasil pertanian maupun peternakan guna mendukung pertanian organik. Kata Kunci: Limbah, Organik, POC, Camplong II,NTT
Promosi kesejahteraan hewan dan higiene sanitasi dalam penyembelihan hewan kurban di Kota Kupang Aji Winarso; Dodi Darmakusuma; Maxs Urias E. Sanam
ARSHI Veterinary Letters Vol. 2 No. 3 (2018): ARSHI Veterinary Letters - Agustus 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.398 KB) | DOI: 10.29244/avl.2.3.57-58

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Tulisan mendeskripsikan pengetahuan masyarakat sasaran sosialisasi tentang implementasi kesrawan dan praktik higiene/sanitasi penyembelihan hewan qurban di Kota Kupang setelah mendapatkan penyuluhan dan pelatihan oleh tim pengabdian kepada masyarakat Universitas Nusa Cendana. Penelitian ini merupakan penelitian survey pada populasi yang diintervensi dengan penyuluhan dan pelatihan dengan menggunakan metode pre test dan post test. Peserta penyuluhan dan pelatihan kesrawan dan higiene daging qurban di Masjid Al Mujahidin Penfui dan Masjid Darul Hijrah Kolhua, Kota Kupang (masing-masing 15 orang), yaitu. Soal pretest dan posttest sebanyak 20 pertanyaan benar dan salah. Data dianalisis secara deskriptif dengan menggunakan Microsoft Excel. Kegiatan penyuluhan dan pelatihan kesrawan dan praktik higiene bagi panitia qurban di kedua masjid berhasil meningkatkan skor pemahaman peserta tentang aspek kesrawan dan praktik higiene dan sanitasi dalam menangani hewan dan daging qurban sebesar 47,5% dan 40,2%. Pemahaman tersebut penting sebagai landasan sikap dan tindakan dalam menghasilkan daging qurban yang aman, sehat, utuh dan halal.
Blood AST and ALT profile of Sumba Ongole cattle Cynthia Dewi Gaina; Maxs U.E. Sanam; Wilmientje M.M. Nalley; Imanuel Benu; Agus Saputra
ARSHI Veterinary Letters Vol. 4 No. 1 (2020): ARSHI Veterinary Letters - Februari 2020
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.793 KB) | DOI: 10.29244/avl.4.1.17-18

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A study was carried out in one of Indonesia native cattle, Sumba Ongole (SO) Cattle for determination of statuses of various biochemical parameters, aspartate aminotransferase (AST or SGOT) and alanine aminotransferase (ALT or SGPT). Apparently blood from randomly selected cattle (n= 36) were collected aseptically. Biochemical analysis of AST and ALT was performed on UPT. Laboratorium Kesehatan, Kupang, NTT. The results showed that the variations of parameters were not significant (p>0.05) between male and female SO cattle. The AST/SGOT (142.56±20.55 U/l) was slightly higher in female compared with the male (137.00±16.24 U/l), while level of ALT/SGPT were 46.44±10.99 U/l in male and 46.41 ± 10.23 U/l in female. Although, the result was not significant (p>0.05), level of AST and ALT in SO cattle was higher compared to standard which is 60-125 U/l for AST and 6.9-35 U/l for ALT. Results showed possibility of seasonal effect on AST/ SGOT and ALT/SGPT level in male and female SO cattle.