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Optimasi Penggunaan Karagenan dan Kalsium Sulfat pada Pembuatan Tahu Sutra dalam Penembangan Pangan Fungsional: Optimization the Use of Carrageenan and Calcium Sulfate in Silken Tofu Production in Functional Food Development Nurheni Sri Palupi; Nindya Atika Indrastuti; Uju; Elvira Syamsir
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 23 No 2 (2020): Jurnal Pengolahan Hasil Perikanan Indonesia 23(2)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v23i2.30973

Abstract

Wedang tahu merupakan olahan tradisional kombinasi antara tahu sutra yang disiram dengan kuah jahe. Kedelai dan jahe yang menjadi bahan baku utama dalam pembuatan wedang tahu memiliki sifat fungsional bagi tubuh bila dikonsumsi. Hal ini menjadikan wedang tahu berpotensi untuk dikembangkan sebagai pangan fungsional. Mutu tekstur tahu sutra menjadi salah satu faktor penentu kualitas wedang tahu. Penambahan karagenan pada pembuatan tahu sutra diharapkan dapat meningkatkan mutu tekstur tahu sutra yang dihasilkan. Tujuan penelitian adalah menentukan kombinasi konsentrasi kalsium sulfat dan karagenan sehingga diperoleh nilai respon rendemen dan tekstur yang optimal menggunakan program Design Expert 7.0. Penentuan formula optimal didasarkan pada hasil pengamatan secara visual (subjektif) yang dikorelasikan dengan hasil pengukuran tekstur secara objektif menggunakan TA-XT2i Texture Analyzer. Formula optimal kemudian dilanjutkan dengan uji sensori, uji proksimat, perhitungan angka kecukupan gizi (AKG), uji tekstur dan uji sineresis. Formula optimal yang direkomendasikan adalah kombinasi kalsium sulfat sebanyak 0,45% dan karagenan 0,10% dengan nilai desirability sebesar 0,75. Formula optimal tersebut memiliki nilai rendemen sebesar 85,36%, kekerasan sebesar 173,60 gf, daya kohesif sebesar 0,07; elastisitas sebesar 0,54; dan daya kunyah sebesar 6,58 gf.
Pengaruh Jenis Pelarut dan Ultrasonikasi terhadap Ekstrak Fikoeritrin dari Kappaphycus alvarezii : The Effect of Different Solvent Type on Phycoerithryn Extract from Kappaphycus alvarezii Anne Mumtaza Putri; Uju; Safrina Dyah Hardiningtyas
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 24 No 2 (2021): Jurnal Pengolahan Hasil Perikanan Indonesia 24(2)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v24i2.37182

Abstract

Kappaphycus alvarezii is a type of red seaweed (Rhodophyta) which contains phycobiliprotein pigments. Red seaweed is dominated by phycoerythrin type phycobiliprotein. Phycoerythrin can be obtained from the extraction process. The purpose of this study was to determine the effect of solvent type and acceleration time of ultrasonication extraction on phycoerythrin pigments. The extraction process was carried out in stages using distilled water, CaCl2 and phosphate buffer with ultrasonication acceleration of 35-55 minutes. The results showed that the phycoerythrin pigment extract of K. alvarezii using distilled water produced a more concentrated pigment color than buffer phosphate and CaCl2, showing the highest concentration of 0.057 mg/mL, and a purity index of 0.189. Water solvent is safe to use because water does not have toxic power. So, there is no need to conduct a preliminary test for solvent toxicity. It is more economical than other types of solvents. The extraction of phycoerythrin pigment using distilled water for 45 minutes resulted in a more concentrated pigment color. The concentration of phycoerythrin pigment before and after precipitation was 0.170 mg/mL and 0.421 mg/mL, respectively. The phycoerythrin pigment purity index before and after precipitation was 0.301 and 0.831, respectively. The best antioxidant activity (% inhibition) was found at concentrations of 50 ppm and 80 ppm phycoerythrin pigment with extraction time of 45 minutes at 62% and 89% respectively. Protein profile using SDS-Page showed that phycoerythrin has a molecular weight of 16.27 kDa in the subunit, 17.79 kDa in the subunit β, and 30.60 kDa in the subunit γ.