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All Journal Jurnal Sain Veteriner
Akrom, Afif Muhammad
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Veterinary

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Swab Bukal Sebagai Bahan Sexing Piyikan Burung Kenari (Serinus canaria) dan Burung Merpati (Columba livia) Akrom, Afif Muhammad; Indarjulianto, Soedarmanto; Yanuartono, Yanuartono; Susmiati, Trini; Nururrozi, Alfarisa; Raharjo, Slamet; Permana, Rief Ghulam Satria; Sitompul, Yeremia Yobelanno
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1012.071 KB) | DOI: 10.22146/jsv.57364

Abstract

Molecular sexing for bird with polymerase chain reaction-based method have been developed, but the samples used as a sources of DNA are blood and feathers which are considerably invasive. The aim of this study was to study the efficiency of buccal swabs as a resource of DNA for sexing canary bird and pigeon. This study used 10 canaries (Serinus canaria) consisting of 6 adult canaries (3 males and 3 females) and 4 young nestling canaries (14 - to 18-day old) and 6 adults (3 males and 3 females) pigeons (Columba livia) and 7 young nestling pigeons (14- to 25-day-old). All birds were taken their buccal swab samples, then DNA were extracted, mixed with PCR-mix to be amplified for sexing genes with CHD1F/CHD1R primer pairs. The amplification results showed that all of adult male birds produced single band (± 500 bp), whereas all of adult female birds produced double bands (± 500 bp and ± 300 bp). The PCR method for nestling canaries showed 2 males and 2 females. whereas nestling pigeons 6 males and 1 female. Based on this study it can be concluded that buccal swabs are efficient as a source of DNA for birds sexing especially young nestling birds. 
Co-Authors A.A. Ketut Agung Cahyawan W Alfarisa Nururrozi, Alfarisa Permana, Rief Ghulam Satria Sitompul, Yeremia Yobelanno Slamet Raharjo Trini Susmiati Yanuartono, Yanuartono