Claim Missing Document
Check
Articles

Found 30 Documents
Search

Aktivitas Metabolit Sekunder Cendawan Endofit terhadap Colletotrichum acutatum pada Cabai Merah Nur Alfi Saryanah; Suryo Wiyono; Dadang Dadang
Jurnal Fitopatologi Indonesia Vol. 15 No. 1 (2019)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (528.248 KB) | DOI: 10.14692/jfi.15.1.36

Abstract

Activity of Fungal Endophyte Secondary Metabolites against Colletotrichum acutatum on Chili PepperColletotrichum acutatum is one of anthracnose causal agents on chili pepper that has been reported to be predominant species in a some regions of Java Island. Secondary metabolites of endophytic fungi have been reported to have a potency as antifungal agents against plant pathogen. However, its antifungal activity against C. acutatum has not been reported yet. This study was aimed to evaluate the antifungal activity of fungal endophyte secondary metabolite against C. acutatum at in vitro and in vivo assay. In vitro assay was conducted to evaluate antifungal activity of fungal endophyte CBR1D14 isolate culture filtrate (FCBR) and mycelia extract (MCBR) in inhibiting conidial germination of C. acutatum. The results of in vitro assay showed that ethyl acetate extract of FCBR (EA FCBR) had the highest activity in inhibiting C. acutatum conidial germination. Methanol fraction from the partition of EA FCBR (FM FCBR) and from the partition of MCBR ethyl acetate extract (FM MCBR) showed the ability in inhibiting C. acutatum conidial germination. In vivo assay to chili pepper fruit showed that the treatment of FM FCBR (IC95 609.9 µg mL-1) and FM MCBR (IC95 1178.27 µg mL-1) decreased anthracnose disease incidence and lesion diameter. The efficacy rate of FM FCBR and FM MCBR treatments against anthracnose was 36.72 and 48.68%, respectively. Bioautography test was done on silica gel thin layer chromatogram. Methanol fraction of FCBR and MCBR were separated into 3 bioautographic spots respectively (Rf 0.04, 0.07, 0.7 for FM FCBR and Rf 0.06, 0.52, 0.7 for FM MCBR).
Aktivitas minyak dan serbuk enam spesies tumbuhan terhadap peneluran dan Mortalitas Callosobruchus sp. (Coleoptera: Bruchidae) Dadang Dadang; Budi Saputro; Kanju Ohsawa
Jurnal Entomologi Indonesia Vol 3 No 2 (2006): September
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.481 KB) | DOI: 10.5994/jei.3.2.59

Abstract

Storage pests remain one of the main problems in storage. The pest attacks occur not only in the field, but also in storage. Control should be done to maintain the quality of storedproducts. The environmentally friendly measures should be implemented to avoid negative impacts to the environment and human being. The objective of this research was to study the mortality and oviposition deterrence caused by powder and oil of six plant species against Callosobruchus sp. (Coleoptera: Bruchidae). Flowers of Eugenia aromatica, roots of Vetiveria zizanioides, leaves of Pogostemon cablin, seeds of Ricinus communis, seeds of Foeniculum vulgare, and stems of Cymbopogon citratus were air-dried and milled to yield powder. Oil of six plant species were purchased from local market. Oviposition deterrent bioassay was conducted by no-choice method for both powder and oil, while mortality bioassay was conducted by topical application and residual methods for plant oil only. Several plant species showed high biological activity to Callosobruchus sp. Powder of E. aromatica and oil of V. zizanioides caused high oviposition deterrence, while oil of V. zizanioides and E. aromatica caused high mortality.
Komunitas Parasitoid Lalat Pengorok Daun pada Pertanaman Sayuran Dataran Tinggi Rustam, Rusli; Rauf, Aunu; Maryana, Nina; Pudjianto, Pudjianto; Dadang, Dadang
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.174 KB) | DOI: 10.31258/jnat.11.1.40-47

Abstract

Leafminer fly, Liriomyza spp. are widely known as new important pests attacking many vegetable species inhigh land in Indonesia. The objective of the research was to study community of parasitoid leafminer in highaltitude, and parasitization level. This research also looked at more specific on parasitization level caused byOpius chromatomyiae. There were four species of leafminer recorded from the survey, Liriomyza huidobrensis,Liriomyza sativae, Liriomyza chinensis and Chromatomyia horticola. The observation also found eight species ofparasitioid associated with collected leafminers. Six species of recorded parasitoid belonged to family of Eulopidae,and two rest species belonged to Braconidae and Eucoilidae respectively. Those eight species of parasitoids wereHemiptarsenus varicornis, Asecodes deluchii, Neochrysocharis okazakii, Neochrysocharis formosa ,Neocrhysocharis sp. Quadrastichus liriomyzae, Opius chromatomyaie, Gronotoma micromorpha. ParasitoidOpius chromatomyiae and Hemiptarsenus varicornis were found to be dominant in the range altitude between1001-1300 and 1301-1600 asl. From 16 spesies of hosts attacked by leaf miners, only Opius chromatomyiae wasrecorded to have ability to parasitize the leafminer in 13 host species with parasitization level reached 1,84–62,26%.
Potensi Insektisida Melur (Brucea javanica L. Merr) dalam Mengendalikan Hama Kubis Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) dan Plutella xylostella (L.) (Lepidoptera: Yponomeutidae) Lina, Eka Candra; Arneti, Arneti; Prijono, Djoko; Dadang, Dadang
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.412 KB) | DOI: 10.31258/jnat.12.2.109-116

Abstract

This study was conducted to evaluate the potency of Brucea javanica (melur) for controlling two species ofcrucifer pests, i.e. Crocidolomia pavonana and Plutella xylostella. Melur fruits, twigs, and leaves were extracteddirectly with methanol or sequentially with hexane, ethyl acetate, and methanol. The most active extract was thenfractionated by preparative layer chromatography using hexane, mixtures of ethyl acetate and methanol, andmethanol as eluents. The most active fraction was formulated as EC (emulsifiable concentrate) and WP (wettablepowder) formulations, and tested for their toxicity and antifeedant effect against C. pavonana and P. xylostellalarvae. The results showed that methanol extract of melur fruits was more active than that of twigs and leaves.Fractionation of methanol extract of melur fruits yielded an active fraction which was eluted with ethyl acetate-methanol 9:1. EC and WP formulations of melur fruits were active against C. pavonana larvae with LC50 of 0.39%and 0.21%, respectively. The same formulations were also active against P. xylostella larvae with LC50 of 0.31% and0.54%, respectively. In no-choice tests, the antifeedant effect of the EC formulation on C. pavonana larvae (feedinginhibition [FI]: 70.9%-97.5%) was higher than on P. xylostella larvae (FI: 52.2%-83.9%), but the antifeedant effect ofthe WP formulation on the two species was relatively the same. In a choice test, the EC formulation at LC 85completely inhibited feeding by C. pavonana larvae (FI: 100%).
Gangguan fisiologi dan biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) akibat perlakuan ekstrak campuran Tephrosia vogelli dan Piper aduncum Eka Chandra Lina; Dadang Dadang; Syafrida Manuwoto; Gustini Syahbirin
Jurnal Entomologi Indonesia Vol 12 No 2 (2015): Juli
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.723 KB) | DOI: 10.5994/jei.12.2.100

Abstract

Plant extracts have been known to cause physiological and biochemical interferences against insect, such as feeding inhibitor, food assimilation, and changes on activity of cytochrome b5 and cytochrome P450. This study was carried out to examine the effect of the extracts mixture of Tephrosia vogeliiand : Piper aduncum (1 : 5) on the physiology of Crocidolomia pavonana (F.) as well as extract of P. aduncum on biochemical of C. pavonana. The study showed that larvae of C. pavonana was experiencing a feeding inhibition until 94.82% after treated with extracts mixture on concentration 0.06% or equivalent to LC95. However, larvae treated with extracts mixture on LC25 and LC50 only experienced a relative growth disorders as a result of intrinsic toxicity of extracts mixture which enters into the body of insects. Insect adaptation to toxic plant compounds indicated by an increase in the digestibility of larvae approximately 11.11%. Furthermore, detoxification mechanism by larvae against active compounds of P. aduncum occured and shown by an increase of oxidative enzyme activity of cytochrome b5 and cytochrome P450, in in vivo and in vitro compared to control. This study provides an information about mode of action of extracts mixture of T. vogelii and P. aduncum (1 : 5) on larvae of C. pavonana and detoxification mechanism by larvae due to extract of P. aduncum
AKTIVITAS INSEKTISIDA EKSTRAK BUAH CABAI JAWA (Piper retrofractum) TERHADAP Helopeltis antonii (HEMIPTERA: MIRIDAE) PRIJONO, DJOKO; DADANG, DADANG; INDRIATI, GUSTI
853-8212
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAKHelopeltis antonii merupakan salah satu hama pada tanaman kakao,teh, dan jambu mete yang menyerang pucuk dan buah dengan menusukkanstiletnya untuk mengisap cairan sehingga menyebabkan kerusakan.Penelitian bertujuan menguji toksisitas ekstrak buah Piper retrofractum(cabai jawa) terhadap imago, pengaruh konsentrasi subletal terhadap nimfaketurunan, persistensi terhadap mortalitas dan oviposisi H. antonii.Penelitian dilaksanakan di Laboratorium Fisiologi dan ToksikologiSerangga, Institut Pertanian Bogor dan Laboratorium Proteksi Tanaman,Balai Penelitian Tanaman Industri dan Penyegar Sukabumi, mulai Mei2013 sampai April 2014. Buah mentimun digunakan sebagai inangpengganti untuk perbanyakan serangga uji H. antonii di laboratorium danmedia pengujian. Hasil penelitian menunjukkan kematian imago H.antonii, akibat perlakuan ekstrak P. retrofractum 0,05-0,3%, sudah terjadipada 24 jam setelah perlakuan (JSP). Sementara itu, pada 24 dan 48 JSPterjadi penurunan tingkat kematian H. antonii. Setelah 48 JSP, hanyaterjadi sedikit peningkatan kematian H. antonii. LC 50 dan LC 95 ekstrak P.retrofractum pada 120 JSP masing-masing 0,20 dan 0,49%. Jumlah nimfaketurunan yang dihasilkan pada perlakuan subletal ekstrak P. retrofractum0,203% (LC 50 ) lebih sedikit dibandingkan dengan konsentrasi 0,141%(LC 25 ). Ekstrak P. retrofractum yang dipajankan di bawah sinar mataharihingga 5 hari masih efektif terhadap imago (mortalitas 80%), tetapi tidakefektif dalam menghambat peletakan telur H. antonii. Penghambatanpeletakan telur terhadap imago H. antonii pada perlakuan ekstrak P.retrofractum 0,98% (2 × LC 95 ) yang dipajankan di bawah sinar matahariselama 0 dan 1 hari, dengan indeks penghambatan oviposisi 22,7 dan23,8%. Keefektifan ekstrak P. retrofractum perlu diuji di lapangan untukmenilai kelayakan dalam pengendalian H. antonii.Kata kunci: insektisida botani, mortalitas, oviposisi, persistensiABSTRACTHelopeltis antonii is cocoa, tea, and cashew nuts important pest thatcauses damage by sucking plant sap from shoots and nuts. This study wasconducted to test toxicity of Piper retrofractum fruit extract on adults,sublethal effect on the production nymphal progeny, and persistenceagainst mortality and oviposition of H. antonii. This study was conductedat The Fisiology and Insect Toxicology Laboratory, Plant ProtectionDepartement, Bogor Agricultural University and The Plant ProtectionLaboratory of Indonesian Industrial and Beverage Crops Research Institutefrom Mey 2013 to April 2014. Cucumber was used as a host substitute forrearing the test insect and as the testing medium. The results show thatadult mortality, due to the P. retrofractum leaf extract treatment 0.05-0.3%, has occured at 24 hours after treatment (HAT). Meanwhile, H.antonii mortality has decreased on 24 and 48 HAT. After 48 HAT, only aslight increased in H. antonii mortality. LC 50 and LC 95 of P. retrofractumextract at 120 HAT were 0.20 and 0.49%, respectively. The treatment atsublethal concentrations (LC 25 and LC 50 ) markedly decreased nymphalprogeny number. P. retrofractum extract suspensions at LC 95 and 2 × LC 95exposed under sunlight for 5 days were still effective against H. antoniiadults (80% mortality), but were not effective in females inhibitingoviposition. The oviposition inhibiting activity was observed only in thetreatment with P. retrofractum extract at 2 × LC 95  exposed under sunlightfor 0 and 1 day in which the oviposition deterrency indices were 22.7 and23.8% respectively. Key words: botanical insecticides, mortality, oviposition, persistence
Analisis Kemampuan Representasi Mahasiswa Dalam Menyelesaikan Soal Fisika Pada Materi Hukum III Newton Dadang, Dadang; Werdhiana, I Komang; Saehana, Sahrul
JPFT (Jurnal Pendidikan Fisika Tadulako Online) Vol 7, No 2 (2019): E-Jurnal Pend. Fisika Tadulako
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.031 KB)

Abstract

Penelitian ini bertujuan untuk mengkaji, mengetahui, dan menganalisis kemampuan representasi mahasiswa dalam menyelesaikan soal fisika pada materi hukum III Newton.  Subyek penelitian ini terdiri dari 22 orang mahasiswa dan dipilih 3 responden dengan cara pemberian tes pemilihan responden dalam bentuk pilihan ganda kemudian dikelompokkan menjadi 3 kategori yaitu kategori tinggi, sedang, dan rendah. Data diperoleh melalui pemberian tes sebanyak dua kali yaitu tes pemilihan responden dan tes kemampuan representasi serta wawancara. Tes terdiri dari tiga nomor soal yang setiap soal terdiri dari 3 konteks berbeda yang diberikan kepada mahasiswa dan setelah itu dilakukan wawancara. Data penelitian dianalisis melalui pendekatan deskriptif-kualitatif. Hasil penelitian menunjukan bahwa kemampuan representasi siswa berbeda-beda dari setiap representasi yang diberikan. Hasil penelitian menunjukkan bahwa mahasiswa masih cenderung lemah dalam menjawab soal dengan format representasi verbal dan format representasi yang dominan dikuasai oleh mahasiswa yaitu format grafik. Perlunya pembiasaan oleh mahasiswa calon guru dalam menyelesaikan soal fisika dengan menggunakan representasi yang beragam yaitu guna membantu dalam mengomunikasikan pemikiran, mengembangkan dan memperdalam pemahaman mereka. 
Penghambatan aktivitas peneluran kumbang kacang hijau Callosobruchus Chinensis L. (Coleoptera: Bruchidae) oleh extrak sepuluh spesies tumbuhan Dadang Dadang; U. Undayasari
Jurnal Entomologi Indonesia Vol 2 No 2 (2005): September
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1389.326 KB) | DOI: 10.5994/jei.2.2.13

Abstract

Oviposition Deterrence of Bean Weevil, Callosobruchus chinensis L.(Coleoptera: Bruchidae) Treated with Ten Plant Extracts. Pest and Diseases attack agricultural products not only in the field but also in storehouse. Their attack causes decreasing both quantity and quality of stored materials. One of important stored product insect pests is Callosobruchus chinensis L. (Coleoptera: Bruchidae). Till now the effective strategy to control this insect pest is chemical control by using synthetic insecticides. The improper use synthetic insecticides causes some undesirable effects, so alternative strategist should be searched to controls insect pests in storehouse. One of the alternatives is by using plant materials as insect pests control agent. The aim of this study was to find out the oviposition deterrence of C. chinensis treated with ten plant which were extracted with methanol, hexane and ether. Oviposition deterrence was evaluated by choice and no-choice methods at 1,3 and 5% of extract concentration. Extract of Acorus calamus (methanol), A.calamus (hexane), A. calamus (ether), Illicium verum (ether), Pogostemon calbin (hexane), P. cablin (ether), Vetiveria zizanioides (hexane), and V. zizanioides (ether) were able to deter ovipostion activity of C. chinensis by more than 90% of deterrence. Further study should be conducted to isolate and identify the active compound and to make botanical insecticide formulation for practical use as a commercial product.
Aktivitas biologi enam jenis ekstrak tumbuhan famili Asteraceae terhadap larva Spodoptera litura Fabricius (Lepidoptera: Noctuidae) Ratna Sari Dewi; Dadang Dadang
Jurnal Entomologi Indonesia Vol 2 No 1 (2005): April
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1388.603 KB) | DOI: 10.5994/jei.2.1.51

Abstract

Biological Activity of Six Plan Extract from Asteraceae on Spodoptera litura Fabricius (lipedoptera : Noctuidae) Larvae. Asteraceae is one of plant family that is known to have insecticidal activity to several insect pests, such as Parthenium argentatum, Crysanthemum cineariaefolium, and Agerantum houstoneanum. The aim of this study is to explore other asteraceae species in other to search for insecticidal activity to Spodoptera litura Fabricius (Lepidoptera: Noctuidae). Six species, Blumea balsamifera (leaf), Elephantopus scaber (leaf), Gynura procumbens (leaf) Artemisia vulgaris (leaf) Soncbus arvensis (leaf) and Helianthus annus (seed) were use in this study. Plant extract were obtained by meseration method using menthanol. The extract were bioassayed to the second instar larvae of S. litura to evaluate the mortality, antifeedant and growth regulation activity. Extract of B. balsamifera and E. scaber have high antifeedant activity at 5 % by reducing larval feeding 87.7% and 81.8% in no choice test, and 94.1% and 86.1% in choice test method, respectively Extract of H. annus, A. vulgaris, and E. scaber prolonged the development of larvae by 4.9, 4.1, 3.9 days, respectively. While extract of H. annus caused mortality of larvae by 86% at 5%.
Perbedaan pola tanam dan kriteria aplikasi insektisida memengaruhi keanekaragaman arthropoda tanah pada pertanaman kubis (Brassica oleracea) Inrianti Sari Handayani; Dadang Hermana; Ali Nurmansyah
Jurnal Entomologi Indonesia Vol 16 No 3 (2019): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.83 KB) | DOI: 10.5994/jei.16.3.163

Abstract

The use of intensive and excessive insecticides on cabbage cultivations causes pest resistance, pest resurgence, environmental pollution, chemical residues, as well as decreasing the population of natural enemies and soil arthropods. Various pest control techniques have been developed to reduce the pest population, two of them are intercropping systems as part of cultural technique strategy and insecticide application. The aim of this research was to study the effect of three cabbage (Brassica oleracea) cultivation patterns and different criteria in insecticide application against soil arthropod diversity including the natural enemy population. The research was designed using split-plot experimental design. The three cultivation patterns consisted of intercropping cabbage with tomato, cabbage, and Ageratum sp. as refugia plant, and monoculture cabbage. Three criteria in insecticide application were economic threshold-based application, calendar system, and without insecticide application. In general, the results showed that cabbage monoculture cultivation and insecticide applications resulted in the lowest soil arthropod population among other treatments. Treatments of cabbage and Ageratum sp. and without insecticide application could increase the diversity of soil arthropods.