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SCREENING BAKTERI TERMOFILIK PENGHASIL ENZIM AMILASE DARISUMBER AIR PANAS SINGGAHAN TUBAN, JAWA TIMURSCREENING THERMOPHILIC BACTERIA OF AMYLASE ENZYME FROMSINGGAHAN HOT SPRINGS TUBAN, JAWA TIMUR EVA NOVITASARI, YULIANA
Unesa Journal of Chemistry Vol 3, No 3 (2014): Volume 3, Number 3, 2014
Publisher : Unesa Journal of Chemistry

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Abstract

Abstrak. Penelitian ini bertujuan untuk memperoleh bakteri termofilik yang menghasilkan amilase darisumber air panas singgahan di Tuban berdasarkan clear zone dan menentukan spesies bakteri yangmempunyai indeks amilolitik tertinggi. Diperoleh 103 isolat dan 51 isolat menunjukkan aktivitas amilasedengan terbentuknya zona bening disekitar koloni pada media padat yang mengandung 1% pati. Indeksamilolitik tertinggi ditunjukkan oleh isolat ST-32 sebesar 3,8 mm. Berdasarkan uji morfologi dan fisiologiisolat ST-32 menunjukkan spesies Thermoactinomyces saachari.Kata kunci: bakteri termofilik, amilase, pati, zona beningAbstract. This research aims to obtain thermophilic bacteria produced amylase from Singgahan hot springin Tuban based on clear zone and determine species of bacteria that have highest amylolytic index. It wasobtained 103 isolates and 51 isolates showed amylase activity with formation clear zone around the colonyin medium containing 1 % starch. The highest amylolytic index was showed by ST-32 isolate is 3.8 mm.Based on morphology and phisiology ST-32 isolates shown species Thermoactinomyces saachari.Keywords: thermophilic bacteria, amylase, starch, clear zone