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All Journal Jurnal Kesehatan Masyarakat Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal SOLMA Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika Bioscientist : Jurnal Ilmiah Biologi
Moh. Mirza Nuryady
Program Studi Pendidikan Biologi, FKIP, Universitas Muhammadiyah Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Mathematics Physics Public Health Social Sciences Other

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DESAIN DAN OPTIMASI PRIMER GEN PENGKODE MRPA TRYPANOSOMA EVANSI DAN PENERAPAN PADA PEMBELAJARAN BIOLOGI MOLEKULER Nuryady, Moh. Mirza; Husamah, H.; Miharja, Fuad Jaya; Hindun, Iin; Patmawati, P.
Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika Vol 4, No 2: July 2020
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (547.422 KB) | DOI: 10.36312/e-saintika.v4i2.217

Abstract

Penelitian molekuler untuk menemukan gen pengkode resistensi Multidrug Resistance Prtotein A (MRPA) T. evansi dan perbanyakan gen secara Polimeration Chain Reaction (PCR)  masih sedikit dilakukan  dan sangat penting untuk dipahami oleh mahasiswa calon guru biologi.  Kajian ini bertujuan untuk menganalisis proses desain dan optimasi primer untuk gen target MRPA T. evansi yang dapat digunakan sebagai sumber belajar mahasiswa pendidikan biologi. Penelitian ini merupakan penelitian deskriptif mengenai tahapan mendesain primer secara online, optimasi primer secara laboratorium serta kajian mengenai pentingnya penerapan hasil studi ini dalam pembelajaran. Hasil penelitian menunjukkan terdapat tiga desain primer yang memenuhi syarat, selanjutnya dari tiga primer tersebut hasil optimasi di laboratorium menunjukkan hanya terdapat dua primer yang menunjukkan hasil yang baik dan dapat digunakan untuk penelitian amplifikasi gen MRPA T. evansi, yaitu primer pertama (F1?, R1?) dan primer kedua (F2?, R2?). Hasil kajian desain dan optimasi primer ini menunjukkan bahwa mahasiswa pendidikan biologi sangatlah penting untuk memahami konsep terkait dengan pekerjaan molekuler seperti mendesain dan optimasi primer, dikarenakan mereka memiliki tuntutan untuk menjadi seorang calon pendidik atau sebagai calon peneliti dimasa depan.Design and Optimization of Trypanosoma evansi MRPA Primer Coding Genes and Application to Molecular Biology LearningAbstractMolecular research to find Multidrug Resistance Prtotein A (MRPA) resistance coding genes and gene propagation by Polimeration Chain Reaction (PCR) is still little done and is very important to be understood by prospective biology teacher students. This study aims to analyze the design and primary optimization process for the T. evansi MRPA target gene that can be used as a learning resource for biology education students. This research was a descriptive study to described the step of primer design and optimization due to the importance of this steps to be applied as learning source. The results showed that there were 3 primer designs that qualified, then after the optimizing step there were only two primers that showed a good result, the first primer (F1, R1) and second primer (F2', R2). The results of this study showed the importance of biology education students to understand the concepts related to molecular work because in the future they are not only become prospective educators, they also have demands as prospective researchers.
PENGARUH EKSTRAK DAUN SERAI (Cymbopogon citratus (DC.) Stapf) TERHADAP PERKEMBANGBIAKAN KUTU BERAS (Sitophilus oryzae L.) Miftachur Rohma; Moh Mirza Nuryady; Sri Wahyuni
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 23, No 2 (2021)
Publisher : Program Studi Ilmu Tanah Fakultas Pertanian UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.23.2.136-145

Abstract

Desain dan Optimasi Primer Gen Pengkode MRPA Trypanosoma evansi dan Penerapan pada Pembelajaran Biologi Molekuler Moh. Mirza Nuryady; H. Husamah; Fuad Jaya Miharja; Iin Hindun; P. Patmawati
Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika Vol. 4 No. 2: July 2020
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/e-saintika.v4i2.217

Abstract

Penelitian molekuler untuk menemukan gen pengkode resistensi Multidrug Resistance Prtotein A (MRPA) T. evansi dan perbanyakan gen secara Polimeration Chain Reaction (PCR)  masih sedikit dilakukan  dan sangat penting untuk dipahami oleh mahasiswa calon guru biologi.  Kajian ini bertujuan untuk menganalisis proses desain dan optimasi primer untuk gen target MRPA T. evansi yang dapat digunakan sebagai sumber belajar mahasiswa pendidikan biologi. Penelitian ini merupakan penelitian deskriptif mengenai tahapan mendesain primer secara online, optimasi primer secara laboratorium serta kajian mengenai pentingnya penerapan hasil studi ini dalam pembelajaran. Hasil penelitian menunjukkan terdapat tiga desain primer yang memenuhi syarat, selanjutnya dari tiga primer tersebut hasil optimasi di laboratorium menunjukkan hanya terdapat dua primer yang menunjukkan hasil yang baik dan dapat digunakan untuk penelitian amplifikasi gen MRPA T. evansi, yaitu primer pertama (F1’, R1’) dan primer kedua (F2’, R2’). Hasil kajian desain dan optimasi primer ini menunjukkan bahwa mahasiswa pendidikan biologi sangatlah penting untuk memahami konsep terkait dengan pekerjaan molekuler seperti mendesain dan optimasi primer, dikarenakan mereka memiliki tuntutan untuk menjadi seorang calon pendidik atau sebagai calon peneliti dimasa depan.Design and Optimization of Trypanosoma evansi MRPA Primer Coding Genes and Application to Molecular Biology LearningAbstractMolecular research to find Multidrug Resistance Prtotein A (MRPA) resistance coding genes and gene propagation by Polimeration Chain Reaction (PCR) is still little done and is very important to be understood by prospective biology teacher students. This study aims to analyze the design and primary optimization process for the T. evansi MRPA target gene that can be used as a learning resource for biology education students. This research was a descriptive study to described the step of primer design and optimization due to the importance of this steps to be applied as learning source. The results showed that there were 3 primer designs that qualified, then after the optimizing step there were only two primers that showed a good result, the first primer (F1, R1) and second primer (F2', R2). The results of this study showed the importance of biology education students to understand the concepts related to molecular work because in the future they are not only become prospective educators, they also have demands as prospective researchers.
Uji Susceptibility Nyamuk Aedes aegypti terhadap Insektisida Malathion di Wilayah Fogging Kabupaten Malang Zada Alimatul Mu’azah; Ainur Rofieq; Mohammad Mirza Nuryady; Tutut Indria Permana; Anka Mohammad Dinindra; Jihan Ully Agustin; Nur Ilmi Dwi Sasmitasari; Muhammad Ahman Luthfi Setiawan; Putri Ayu Irrodah
Bioscientist : Jurnal Ilmiah Biologi Vol 9, No 2 (2021): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v9i2.4051

Abstract

Malang Regency is an endemic area for cases of DHF (Dengue Hemorrhagic Fever) in East Java Province. One of the efforts to reduce the number of cases is to control the breeding of the Aedes aegypti mosquito which is the main vector. However, this will depend on the susceptibility status of Aedes aegypti to the insecticides used, one of which is malathion. This study aims to determine the susceptibility status of Aedes aegypti mosquitoes to malathion insecticides in Malang Regency in the southern part. This descriptive observational study used adult Aedes aegypti mosquitoes obtained from the landing collection of larvae from three sub-districts in Malang Regency in the southern part (Kepanjen, Gondanglegi, and Bantur). The location selection was done by simple random sampling technique. The larvae of the Aedes aegypti mosquito were then reared in the Chemistry Laboratory, University of Muhammadiyah Malang. Adult mosquitoes with stomach conditions full of sugar were then tested using the susceptibility test which refers to WHO standards. The results showed that the mortality rate of the tested mosquitoes in the Districts of Kepanjen, Gondanglegi, and Bantur, respectively, reached 98%, 93%, and 96%. This shows that the Aedes aegypti mosquitoes in the three locations are classified as tolerant to the insecticide malathion by 0.8%. Thus, it can be concluded that the insecticide malathion is still effectively used as vector control for DHF (Dengue Hemorrhagic Fever) in Malang Regency.
Strengthening Teenage Scientific Work during the COVID-19 Pandemic to Improve Youth Scientific Literacy Writing Skills Diani Fatmawati; Moh. Mirza Nuryady; Ilma Nurul Khoiriyah; Anggitania Dyan Anggraini; Lisma Dahlia
Jurnal SOLMA Vol. 11 No. 1 (2022)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v11i1.8058

Abstract

Background: Karya ilmiah dan literasi remaja merupakan keterampilan yang penting untuk dimiliki oleh generasi abad 21. Metode: Mitra dalam kegiatan pengabdian ini adalah siswa dan pengasuh Panti Asuhan Putri ‘aisiah Riverside yang keseluruhan berjumlah 15 orang. Metode pengabdian yang dilakukan adalah dengan Focus Group Discussion (FGD), pembentukan kelompok KIR, serta pembuatan KIR. Pengumpulan data dilakukan dengan menggunakan instrumen pre-test serta rubrik penilaian artikel karya ilmiah remaja. Hasil: Hasil program pengabdian menunjukkan bahwa pemahaman awal siswa tentang KIR tergolong. Selanjutnya perolehan skor awal KIR yang dihasilkan oleh dua kelompok siswa yang terpilih untuk lomba karya tulis ilmiah (LKTI) remaja nasional hanya mencapai 36 dan 53. Namun, setelah pendampingan yang dilakukan oleh tim pengabdian masyarakat, skornya meningkat signifikan mencapai 70 dan 95 sehingga salah satu dari dua kelompok tersebut memenangkan LKTI nasional sebagai juara ketiga. Kesimpulan: Pendampingan untuk memperkuat keterampilan remaja dalam membuat karya ilmiah dan literasi sangat disarankan selama pandemi COVID-19.
Analisis Status Resistensi Aedes aegypti terhadap Insektisida Cypermethrin 0,05% di Kecamatan Endemis Kabupaten Malang Siti Mariyatul Qibtiyah; Moh. Mirza Nuryady; Rr. Eko Susetyarini; Tutut Indria Permana; Dhiga Agung Sasongkojati
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 1 (2022): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i1.4988

Abstract

Aedes aegypti mosquitoes was the primary vector for carrying dengue fever. Dengue vector control has been widely carried out to reduce the number of dengue cases in Indonesia, but resistance cases have occurred in several areas, so it was necessary to know the status of resistance to determine appropriate efforts to control the vector. The purpose of this study was to determine the resistance status of Aedes aegypti in Malang Regency to 0.05% Cypermethrin. This type of research was descriptive observational research. The research method used was the susceptibility method with WHO standards using impregnated paper with the insecticide 0.05% Cypermethrin. Aedes aegypti mosquitoes were obtained from ovitrap results in the form of eggs and larvae taken from rearing at the Chemical Laboratory of the University of Muhammadiyah Malang. The number of mosquitoes used was 400 mosquitoes of the Aedes aegypti species aged 3-5 days. Mosquitoes were contacted with impregnated paper 0.05% Cypermethrin for one hour and held for 24 hours. Based on WHO standards, the criteria for susceptibility were determined as follows: said to be vulnerable if mortality was >98%, said to be tolerant if mortality was between 80-98%, and said to be resistant if mortality was <80%. The results showed that Aedes aegypti in Malang Regency was tolerant to 0.05% Cypermethrin with death rate each district; Turen 90%, Kepanjen 90%, Karangploso 96%, and Dau 100%, with percentage of 94%. This research showed that Cypermethrin with a concentration of 0.05% was still effective in reducing the development of Aedes aegypti, but the rotation of use must still be considered to reduce the number of dengue cases.
Bintaro Leaves (Cerbera manghas): Toxicity to Aedes aegypti Instar III Larvas Permana, Tutut Indria; Sasmitasari, Nur Ilmi Dwi; Susetyarini, Eko; Nuryady, Moh. Mirza; Dinindra, Anka Muhammad; Agustin, Jihan Ully; Lutfi, Muhammad Ahman; Ayu, Putri; Alimatul, Zada
KEMAS: Jurnal Kesehatan Masyarakat Vol 17, No 4 (2022)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v17i4.27670

Abstract

DHF cases tend to increase from year to year in Indonesia. So the government makes efforts to control cases of Dengue Fever (DHF) which are usually done chemically and harm the environment and health. There is a need for safer, more effective, and environmentally friendly controls, such as using natural ingredients as natural larvicides. Among the natural ingredients having potential as a larvicide is Bintaro leaves (Cerbera manghas). The purpose of this study was to determine the toxicity of Bintaro leaf extract against third instar larvae of Aedes aegypti mosquitoes. This experimental study used 7 treatments of Bintaro leaf extract concentration (5 %, 15 %, 25 %, 35 %, 45 %, 65 %, 75 %), abate as a positive control, and distilled water as a negative control. Each treatment used ten instar III Aedes aegypti larvae with four repetitions. The data obtained were then analyzed using probit analysis to determine the toxicity of Bintaro leaf extract to Aedes aegypti larvae by calculating the LC50 and LC90 values. The results showed that the most effective concentration was 75 % because it could kill 100% of the test larvae. The LC50 value of 5,097 % and the LC90 value of 25,300 % indicate that the level of toxicity is very toxic. The probit regression analysis shows a linearity line equation y = 1.15 + 1.43x with a correlation (R2) of 0.512 which indicates that the correlation is strong enough. It is related to the content of flavonoids, tannins, saponins, triterpenoids which are toxic to the abdomen, nervous system, and respiratory system of larvae. From the research results, Bintaro extract with a concentration of 75 % can be used as a natural larvicide candidate. Furthermore, further research to see the toxicity to the environment can be done.are toxic to the abdomen, nervous system, and respiratory system of larvae. From the research results, Bintaro extract with a concentration of 75 % can be used as a natural larvicide candidate. Furthermore, further research to see the toxicity to the environment can be done.
Co-Authors Agustin, Jihan Ully Ainur Rofieq Alimatul, Zada Anggitania Dyan Anggraini Anka Mohammad Dinindra Ayu, Putri Dhiga Agung Sasongkojati Diani Fatmawati Dinindra, Anka Muhammad Fuad Jaya Miharja Husamah Husamah Iin Hindun Iin Hindun Ilma Nurul Khoiriyah Jihan Ully Agustin Lisma Dahlia Lutfi, Muhammad Ahman Miftachur Rohma Muhammad Ahman Luthfi Setiawan Nur Ilmi Dwi Sasmitasari P. Patmawati Patmawati, P. Putri Ayu Irrodah Rr. Eko Susetyarini Sasmitasari, Nur Ilmi Dwi Siti Mariyatul Qibtiyah Sri Wahyuni Tutut Indria Permana Zada Alimatul Mu’azah