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All Journal Jurnal Farmasi Udayana Jurnal Midpro Sainstech Farma: Jurnal Ilmu Kefarmasian
Munawarohthus Sholikha
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Phytochemical Screening And Tyrosinase Inhibition Activity Of Leaves Cassia siamea L. Munawarohthus Sholikha; Ainun Wulandari
Jurnal MIDPRO Vol 12, No 2 (2020): JURNAL MIDPRO
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/md.v12i2.240

Abstract

Cassia siamea L. has been used traditionally as medicine and in previous studies had an IC­50 antioxidant activity of 144.12 µg / mL. Cassia siamea L. potential as a medicinal substance is thought to have chemical constituents such as saponins, anthraquinones, alkaloids, flavonoids, tannins, terpenoids and steroids. Indonesia, which is located in a tropical region with high temperatures and ultraviolet (UV) radiation, can cause skin disorders such as hyperpigmentation due to excess melanin synthesis. Tyrosinase enzymes can prevent or inhibit melanin formation. The purpose of this study was to determine the chemical content of Cassia siamea L. leaves extract with various solvents and the inhibitory activity of tyrosinase enzymes. In this study, the leaves of Cassia siamea L. were macerated with methanol as a solvent. The dry methanol extract was fractionated using the liquid-liquid method using aquadest, butanol and chloroform as solvents. The four dried extracts were then phytochemical screening to determine their chemical content. After that, the tyrosinase inhibition test was carried out in vitro with L-Dopa as a substrate using ELISA plate well reader at concentrations of 100, 1000 and 100000 ppm with three repetitions (triplo). The methanol extract had percent inhibition of 19.993 ± 1.125%, 28.984 ± 0.624%, 57.164 ± 0.623%, respectively; butanol fraction 25.914 ± 0.541%, 32.566 ± 0.767%, 52.120 ± 1.616%; chloroform fraction 19.920 ± 0.730%, 26.425 ± 0.937%, 62.865 ± 0.167% and aquadest fraction 23.830 ± 0.879%, 30.885 ± 0.778%, 67.471 ± 0.352%. Kojic acid had tyrosinase activity and acts more active than the other extract and fraction with 91.155±0.228% at 500 ppm. The butanol fraction had the highest percent inhibition at 100 and 1000 ppm which was thought to be due to the presence of alkaloids, tannins, flavonoids, steroids and terpenoids from the results of the phytochemical screening test.
Uji Aktivitas Ekstrak Daun Dan Akar Singawalang (Petiveria alliaceae) Terhadap Penghambatan Tirosinase Munawarohthus Sholikha; Nur M, R.; Mahfudza, A.R.
Jurnal Farmasi Udayana Vol. 10, No 2, Tahun 2021
Publisher : Departement of Pharmacy, Faculty of Mathematics and Natural Science, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JFU.2021.v10.i02.p14

Abstract

Tirosinase merupakan salah satu enzim yang berperan dalam pembentukan pigmen kulit dari seseorang karena terlibat dalam proses melanogenesis. Tirosinase sangat berperan terhadap proses depigmentasi kulit, terdapat beberapa upaya untuk menghambat proses depigmentasi kulit salah satunya dengan menghambat tirosinase. Penelitian pada daun dan akar singawalang (Petiveria alliacea) dilakukan untuk mengetahui adanya potensi sebagai inhibitor tirosinase. Ekstrak daun dan akar singawalang dimaserasi dengan etanol, kemudian dilakukan uji identifikasi metabolit sekunder. Ekstrak daun singawalang mengandung alkaloid, tanin dan terpenoid sedangkan ekstrak akar singawalang mengandung alkaloid, flavonoid, tanin dan terpenoid. Aktivitas penghambatan tirosinase menggunakan teknik microplate reader ELISA pada panjang gelombang 492 nm. Pengujian dilakukan terhadap asam kojat sebagai pembanding dan L-DOPA sebagai substrat. Hasil uji aktivitas penghambatan tirosinase ekstrak daun singawalang, akar singawalang dan asam kojat, berturut-turut IC50 sebesar 9,817 mg/mL, 4,987 mg/mL dan 0,092 mg/mL.
Analisis Kandungan Logam Timbal (Pb), Besi (Fe) Dan Magnesium (Mg) Pada Pakan Ayam Ras Petelur Dengan Metode Spektrofotometri Serapan Atom (SSA) Munawarohthus Sholikha; Fauziah Cinta Natasya; Lia Puspitasari
SAINSTECH FARMA Vol 14 No 2 (2021): Sainstech Farma: Jurnal Ilmu Kefarmasian
Publisher : FAKULTAS FARMASI, INSTITUT SAINS DAN TEKNOLOGI NASIONAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37277/sfj.v14i2.1014

Abstract

Pakan dibutuhkan oleh ayam ras petelur, namun dalam pakan tersebut dapat mengandung unsur logam seperti timbal, besi, magnesium, dan lain–lain. Hal ini akan berpengaruh terhadap produk ternak yang dihasilkan dari ayam tersebut. Jika unsur–unsur logam tidak terkontrol akan memberikan efek negatif untuk manusia yang mengonsumsi telur tersebut seperti kanker dan penyakit lainnya. Untuk mengetahui unsur–unsur logam yang terdapat di dalam pakan, dilakukan pengujian dengan metode Spektrofotometri Serapan Atom (SSA). Sampel uji diperoleh dari dua distributor berbeda yaitu sampel A dan sampel B yang terletak di daerah Jakarta. Dari pengujian tersebut didapatkan hasil, logam Pb (sampel A = 0,0276 mg/kg dan sampel B = Tidak Terdeteksi), logam Fe (sampel A = 134,79 mg/kg dan sampel B = 117,9 mg/kg), logam Mg (sampel A = 413,6 mg/kg dan sampel B = 363,7 mg/kg). Hasil dari pengujian menunjukkan bahwa pakan tersebut mengandung logam Pb tetapi masih di bawah nilai ambang batas, sedangkan kandungan logam Fe dan Mg nilainya berada di atas nilai ambang batas yang direkomendasikan oleh SNI 0,05 mg/kg.
Uji Aktivitas Penghambatan Enzim Tirosinase Oleh Ekstrak Etanol Daun Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Secara In Vitro Munawarohthus Sholikha; Wahyuningtyas Wahyuningtyas; Lia Puspitasari
Sainstech Farma: Jurnal Ilmu Kefarmasian Vol 16 No 1 (2023): Sainstech Farma: Jurnal Ilmu Kefarmasian
Publisher : LPPM, INSTITUT SAINS DAN TEKNOLOGI NASIONAL

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hyperpigmentation is an event that occurs due to excessive production of skin pigment. Skin color is strongly influenced by the presence of melanin, where the presence of melanin is strongly influenced by the enzyme tyrosinase. Taro leaf (Typhonium flagelliforme (Lodd.) Blume) are one of the plants have flavonoid compounds used as antioxidants and tyrosinase inhibitors. The aim of this study was to test the ethanol extract of taro leaf as a tyrosinase inhibitor. Taro leaf in this study were obtained through an extraction process using 96% ethanol solvent by maceration method. The thick ethanol extract of taro leaf was tested for phytochemical screening, total flavonoid levels, and tyrosinase inhibition test. The method used for tyrosinase inhibition test was enzymatic method in vitro. The results of this study indicated that there was a total flavonoid compound content of 0.7378% (b/b) and the inhibitor activity can be seen from the IC50 value for the diphenolation reaction (L-DOPA substrate) which was 13.307 mg/mL. This value was greater when compared with 0.093 mg/mL of phenolic acid, so that the ethanol extract of taro leaf has the potential to inhibit tyrosinase activity.
Sintesis Analog Kurkumin (2E,6E)-2-(4-Hidroksi-3-Metoksi-5-((Pirolidin)metil)benziliden) -6-(4-Metoksibenziliden)Sikloheksan-1-on Nur Rahmawati; Adita Silvia Fitriana; Munawarohthus Sholikha
Sainstech Farma: Jurnal Ilmu Kefarmasian Vol 16 No 2 (2023): Sainstech Farma: Jurnal Ilmu Kefarmasian
Publisher : LPPM, INSTITUT SAINS DAN TEKNOLOGI NASIONAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37277/sfj.v16i2.1578

Abstract

Curcumin is a potential compound to be developed. However, the clinical use of these compounds is limited due to their low stability and bioavailability. Therefore, to increase the pharmacological activity of curcumin, it is necessary to modify the chemical structure of curcumin. Curcumin analog compound (2E,6E)-2-(4-hydroxy-3-methoxy-5-((pyrrolidine)methyl)benzylidene)-6-(4-methoxybenzyliden)cyclohexane-1-on can be synthesized in 3 steps. Phase I, the reaction between p-methoxybenzaldehyde and cyclohexanone took place aldol condensation reaction with NaOH catalyst. Phase II, the Clasein-Schmidt reaction between compound 1 (the result of the synthesis of stage I) and vanillin involved dilute HCl as the catalyst. Phase III, reacting the results of the synthesis of phase II with Mannich pyrrolidine base by adapting the method used by Geschickter and Maeadow. Compound 3 as a result of purification was observed using Thin Layer Chromatography using Ethyl Acetate:Methanol (1:1) as an eluent to obtain Rf 0.46 and a melting range of 130-131 oC. The yield obtained was 51.52% with the characteristics of a reddish brown powder. This compound needs further identification using the Mass Spectrum and NMR.
Co-Authors Adita Silvia Fitriana Ainun Wulandari Fauziah Cinta Natasya Lia Puspitasari Lia Puspitasari Mahfudza, A.R. Nur M, R. Nur Rahmawati Wahyuningtyas Wahyuningtyas