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PENGARUH BERBAGAI KONSENTRASI SARI DAGING BUAH APEL (Molus domestica) TERHADAP KARAKTERISTIK DAN HASIL UJI ORGANOLEPTIK NATA DE APPLE C. Sudarwati; Susie Amilah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 7 No 01 (2014)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol7.no1.a507

Abstract

This study aims to prove the effect of the concentration of fruit juice puch as nata de apple production materials to the production of nata,suppleness and organoleptic characters.The design used in this study was a completely randomized design (CDR) consisting of 3 perlakuan namely 500 gr,750 gr, 1000 gr.1 liter each were taken and repeated 6kali and added sugar 10%, urea 0,5% and 10% starter.The results showed the concentr ation of water and fruit juice apple significantly (p>0,05) on the influence the textur of nata with the highest combination of treatment 750 gram,and the lowest yield on treatment 500 gram.Concentrasi of water and fruit apple was also significanty (p>0,05) on the production of nata with combination treatment 750 gram,for odor and flavor character,but for the color of the panelists preferred the combination treatment nata 500 gram. Keywords: Molus domestica, Nata, Production, Texture organoleptik
ROLE OF IMMUNOGLOBULIN G (IgG) FROM THE INDUCTION OF Escherichia coli PILI ADHESION PROTEIN ISOLATED FROM INFERTILE MALE SEMEN WITH 32.2 KDA MOLECULAR WEIGHT AS OPSONIN AND ANTI-ADHESION AN IN VITRO Escherichia coli INFECTION Sukarjati Sukarjati; Susie Amilah
Folia Medica Indonesiana Vol. 53 No. 2 (2017): JUNE 2017
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.031 KB) | DOI: 10.20473/fmi.v53i2.6343

Abstract

Escherichia coli (E. coli), a major cause of male genital tract infections, is asymptomatic and may result in male infertility. We have succeeded in isolating and characterizing proteins of E. Pili coli isolates from semen of infertile men who function as adhesin with a molecular weight (MW) 32.2 kDa. This study aims to prove the ability of IgG results adhesion proteins induced pili of E. MW coli 32.2 kDa as opsonin to determine the value of the activity and phagocytic capacity and as an anti- adhesion by calculating the average number of E. coli that attached to human spermatozoa. E. coli infertile men's semen were cultured using standard bacteriology. Peritoneal macrophages were isolated from mice. Spermatozoa from donors were prepared using Sil with Select Plus. IgG was obtained from mice immunized with (1) PBS (control), (2) E. coli pili adhesion protein isolated from infertile men semen with MW of 32.2 kDa and (3) weakened E. coli isolated from infertile men's semen. Phagocytic activity value was determined by counting the number of cells activated macrophage phagocytosis process in 100 cells. Phagocytic capacity value was determined by counting the number of bacteria ingested by 25 macrophages. Anti-adhesion test was done by counting the number of bacteria attached to 100 spermatozoa. The results of this study showed difference (p=0.000) in phagocytic activity and phagocytic capacity (p=0.000) between treatment (1) and (2), and between treatment (1) to (3). However, treatment (2) and (3) did not differ neither in phagocytic activity (p=0.693) nor in phagocytosis capacity (p=0.125). Anti-adhesion test produces difference (p=0.000) in the number of E. coli that attached to human spermatozoa between treatments (1) and treatment (2), and between treatments (1) and (3). The number of E. coli that attached to human spermatozoa between treatment (2) and treatment (3) was not significantly different (p=0.371). In conclusion, IgG from the induction of E. coli pili adhesion protein of infertile men semen isolates with MW of 32.2 kDa can increase phagocytic activity and capacity as well as serve as an anti- adhesion. Thus, IgG from the induction of E. coli pili adhesion protein of infertile men semen isolates with MW of 32.2 kDa is protective against in vitro E. coli infection, so that it can be used as material to prevent male reproductive tract infections due to E. coli.
EFEKTIVITAS EKSTRAK DAUN MENGKUDU (Morinda citrifolia L.) DAN DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav) TERHADAP ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus F.E Afiff; Susie Amilah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 01 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no1.a635

Abstract

Has conducted research on the effectiveness of various concentrations of noni leaf extract (Morinda citrifolia L.) And red betel leaf (Piper crocatum Ruiz & Pav) the growth inhibition zone of staphylococcus aureus. Inhibition test using paper disc diffusion method. Paper discs soaked in noni leaf extract and red betel leaf for 30 minutes and placed on media that has been spread bacteria staphylococcus aureus and then incubated for 24 hours. The results showed that noni leaf extract and red betel leaves with a concentration of 40%, 60% and 80% affecting large growth inhibition zone of Staphylococcus aureus.
PENGARUH LAMA PENYIMPANAN INFUSED WATER LEMON (Citrus limon) dan MENTIMUN (Cucumissativus L) TERHADAP PERTUMBUHAN BAKTERI M. I. Chandra; Susie Amilah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1033

Abstract

This study aims to determine the effect of long storage of infused water lemon (Citrus limon) and cucumber (Cucumissativus L) on bacterial growth. Infused water will be stored for 0 hours, 12 hours, 24 hours, 36 hours, and 48 hours at room temperature. This research uses experimental method. The sample used in this research is infused water lemon and infused water cucumber. The research was conducted in Bacteriology laboratory of Surabaya Health Analyst Department in January 2017 - March 2017. Data analysis was done by observing bacterial colony using total plate number by comparing the two sample treatments. Then proceed with statistical test Independent sample t-test about colony calculation results on infused water lemon and infused water cucumber. The final results show that there is an increase in the number of bacteria in the infused water lemon and infused water cucumber every hour. This increase in the number of bacteria can occur due to improper infusion of water storage, and several factors affecting infused water damage. Keywords : Infused water lemon, infused water cucumber, storage duration, bacterial growth, Total Plate Number.
PENGARUH EKSTRAK ETANOL DAUN KECUBUNG GUNUNG (Brugmansia suaveolens) DAN DAUN BINTARO (Cerbera maghas) TERHADAP MORTALITAS LARVA NYAMUK Anopheles sp. A. Kesumawardhani; Susie Amilah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 7 No 02 (2014)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol7.no2.a511

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun kecubung gunung (Brugmansia suaveolens) dan daun bintaro (Cerberra maghas) terhadap mortalitas larva nyamuk Anopheles sp. Sebanyak 1000 larva Anopheles sp tahap instar III dibagi menjadi 2 kelompok. Setiap kelompok mendapatkan perlakuan penambahan ekstrak daun sebesar 0, 1, 2, 3, dan 4 g/l. Masing-masing perlakuan menggunakan 25 larva uji dengan 4 kali pengulangan. Hasil uji aktivitas larvasida menunjukkan penambahan masing-masing ekstrak daun berpengaruh terhadap kematian larva nyamuk Anopheles sp. Aktivitas larvasida kedua kelompok semakin meningkat seiring dengan meningkatnya konsentrasi masing-masing ekstrak etanol daun. Semua perlakuan (1, 2, 3,4 g/L) kedua kelompok berbeda signifikan dengan kontrol (0 g/L), kecuali perlakuan ekstrak etanol daun kecubung gunung 1 g/L. Penamb ahan ekstrak etanol daun kecubung gunung dan daun bintaro pada konsentrasi 4 g/l rata-rata mampu membunuh larva berturut-turut sebanyak 21 dan 23 ekor. Penelitian ini menyimpulkan, bahwa ekstrak daun kecubung gunung dan ekstrak daun bintaro terbukti memiliki aktivitas larvasida terhadap larva nyamuk Anopheles sp, sehingga dapat digunakan masyarakat sekitar sebagai altenatif pengganti abate dalam memberantas larva nyamuk Aedes sp. Kata kunci : kecubung gunung, bintaro, Anopheles, dan larvasida.
AKTIVITAS LARVASIDA EKSTRAK DAUN BANDOTAN (Ageratum conyzoides L.) DAN BUNGA KENANGA (Cananga odorata L.) TERHADAP NYAMUK DEMAM BERDARAH (Aedes Aegypti L.) Indarti Indarti; Susie Amilah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 7 No 02 (2014)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol7.no2.a514

Abstract

Kontrol penyebaran penyakit demam berdarah dapat dilakukan melalui pemberatasan larva nyamuk Aedes aegypti. Penelitian ini bertujuan untuk mengetahui aktivitas larvasida ekstrak daun bandotan (Ageratum conyzoides L.) dan bunga kenanga (Cananga odorata L.) pada larva nyamuk Aedes aegypti L. Sebanyak 500 ekor larva Aedes aegypti instar III dibagi menjadi 2 kelompok masing-masing 250 ekor. Kelompok pertama dibagi 5, masing-masing 25 ekor dimasukan ke dalam toples kaca dan disemprot ekstrak daun bandotan dengan konsentrasi 0, 1,0, 2,0, 3,0, dan 4,0 g/l. Kelompok kedua dibagi 5, masing-masing 25 ekor dimasukan ke dalam toples kaca dan disemprot ekstrak bungan kenanga dengan konsentrasi 0, 1,0, 2,0, 3,0, dan 4,0 g/l dalam air. Hasil penelitian memperlihatkan bahwa penyemprotan ekstrak daun bandotan dan ekstrak bunga kenanga berpegaruh signifikan (P<0,05) terhadap mortalitas larva nyamuk A aegypti. Mortalitas tertinggi larva nyamuk A aegypti diperoleh pada penyemprotan ekstrak daun bandotan dan ekstrak bunga kenanga 4,0 g/l dengan rata-rata mortalitas larva masing-masing 18 ekor dan 22 ekor larva. Hasil uji-t menunjukan bahwa mortalitas larva nyamuk A aegypti yang disemprot ekstrak bunga kenanga signifikan (P<0,0%) lebih tinggi dibandingkan mortalitas larva nyamuk A aegypti yang disemprot ekstrak daun bandotan. Dapat di simpulkan bahwa ekstrak daun bandotan dan bunga kenanga mempunyai aktivitas larvasida pada pada larva nyamuk Aedes aegypti L. Aktivitas larvasida ekstrak bunga kenanga pada larva nyamuk Aedes aegypti lebih tinggi dibandingkan aktivitas larvasida daun bandotan. Kata kunci: daun bandotan, bunga kenanga, Aedes aegypti, dan mortalitas
PELATIHAN PEMBUATAN SABUN PADAT SPIRULINA UNTUK SISWA SMA/SMK DI SURABAYA DAN SIDOARJO Binawati, Diah Karunia; Sukarjati; susie Amilah
Jurnal Penamas Adi Buana Vol 4 No 2 (2021): Jurnal Penamas Adi Buana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/penamas.vol4.no2.a2701

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Abstrak Spirulina adalah sianobakterium multiseluler, walaupun mirip dengan bakteri tetapi Spirulina mempunyai mekanisme fotosintesis yang mirip dengan yang ditemukan pada tanaman. Spirulina merupakan mikroalga sel tunggal yang hidup di air dan berkembang biak dengan cara membelah diri ini dikenal sebagai sumber nutrisi nabati terlengkap yang kaya akan asam amino esensial, vitamin, mineral dan karotenoid (Sitorus, 2015). Kandungan nutrisi Spirulina yang lengkap mengakibatkan Spirulina semakin banyak digunakan untuk kebutuhan manusia baik dalam bidang pangan maupun kecantikan. Pelatihan pembuatan sabun padat transparan Spirulina bertujuan untuk memberikan pengetahuan dan ketrampilan kepada siswa SMA/SMK di Surabaya dan Sidoarjo tentang pengaplikasian ilmu dan teknologi dalam menghasilkan sabun padat transparan untuk membersihkan dan menyehatkan kulit wajah. Bahan yang digunakan adalah Chip soap 450 g, Lexaine C 0,06 g, Carbowax liquid 0,03 g, Propilen glikol 0,03 g, Fixolite 12 g, Spirulina 9 g, Alkohol 96 % 30 ml, parfum. Hasil pelatihan ini memberikan informasi tentang penggunaan Spirulina untuk pembuatan sabun padat transparan dan mempraktekkan secara langsung pembuatan sabun padat transparan, sehingga diharapkan dapat menumbuhkan jiwa entrepreneur siswa. Kata kunci: sabun padat transparan, kulit wajah, Spirulina Abstract Spirulina is a multicellular cyanobacterium, although similar to bacteria, but Spirulina has a photosynthesis mechanism that is similar to those of plants. Spirulina is a single cell microalga that lives in the water and reproduce by dividing itself. Spirulina is known as the most complete source of vegetable nutrition because it’s rich of essential amino acid, vitamin, mineral, and carotenoids (Sitorus, 2015). The complete nutrition in Spirulina makes it more widely known and used to fulfill the human needs, both in the fields of food and beauty. The training of making Spirulina transparent solid soap aims to provide knowledge and skills to students of high school and vocational school in Surabaya and Sidoarjo regarding the application of science and technology in producing transparent solid soap to cleanse and nourish the facial skin. The ingredients used are Chip soap 450 g, Lexaine C 0,06 g, Carbowax liquid 0,03 g, Propilen glikol 0,03 g, Fixolite 12 g, Spirulina 9 g, Alcohol 96% 30 ml, and Perfume. The purpose of this study is to give information and direct practice about the use of Spirulina to make transparent solid soap, so this training is expected to grow the entrepreneurial sense of the students. Keywords: transparent solid soap, facial skin, Spirulina
Potensi Ekstrak Daun Sawo Manila (Manilkara zapota) Dan Daun Sawo Kecik (Manilkara kauki) Terhadap Zona Hambat Pertumbuhan Candida albicans Susie Amilah; Ajiningrum, Purity Sabila; Airin Aisyah
Journal of Pharmacy and Science Vol. 5 No. 2 (2020): Journal of Pharmacy and Science
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v5i2.166

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui potensi dan konsentrasi terbaik dari ekstrak daun sawo manila (Manilkara zapota) dan daun sawo kecik (Manilkara kauki) terhadap zona hambat pertumbuhan Candida albicans. Penelitian ini merupakan penelitian eksperimental menggunakan rancangan acak lengkap (RAL) dengan metode difusi cakram dan dilakukan di Laboratorium Mikrobiologi Fakultas MIPA Universitas PGRI Adi Buana Surabaya. Hasil penelitian menunjukkan bahwa ekstrak daun sawo manila (Manilkara zapota) dan daun sawo kecik (Manilkara kauki) berpotensi dalam menghambat pertumbuhan Candida albicans. Ekstrak daun sawo manila (Manilkara zapota) yang terbaik dalam menghambat pertumbuhan Candida albicans yaitu pada konsentrasi 80% dengan diameter zona hambat 1,62 mm. Ekstrak daun sawo kecik (Manilkara kauki) yang terbaik dalam menghambat pertumbuhan Candida albicans yaitu pada konsentrasi 80% dengan besar zona hambat 3,96 mm.
EFEKTIFITAS EKSTRAK DAUN AFRIKA (Vernonia amygdalina delile) DAN DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) TERHADAP ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus Lailatul Chusniyah; Susie Amilah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 9 No 02 (2016)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol9.no2.a336

Abstract

Has conducted research to determine the influence of Afrika Leaf Extract (Vernonia amygdalina delile) and Binahong Leaf Extract (Anredera cordifolia (Ten.) Steenis) against Staphylococcus aureus growth inhibition zone. The purpose of this study was to determine the effectiveness of Afrika leaf extract (Vernonia amygdalina delile) and leaves Binahong (Anredera cordifolia (Ten.) Steenis) against Staphylococcus aureus growth inhibition zone. The material used in this study is the bacterium Staphylococcus aureus, variations in the concentration of leaves and leaf binahong Afrika. The method used in this research is the diffusion disc with a completely randomized design (4 treatments 6 replications). Leaves Afrika have active compounds are efficacious as antibacterials are flavonoids, tannins, saponins and terpenoids. Binahong Leaf Extract has antibacterial compounds are flavonoids, tannins, alkaloids and essential oils. The results showed that the leaf and leaf afrika binahong with concentrations of 0%, 40%, 60% and 80%. Has an influence on the growth inhibition zone of Staphylococcus aureus. Keywords: inhibition zone, extract, Afrika leaf, leaf binahong, Staphylococcus aureus
Penentuan Jumlah Bakteri Daging Ayam Broiler (Gallus domesticus) Setelah Direndam Dalam Larutan Lidah Mertua (Sansevieria trifasciata) Tri Novita Sari; Susie Amilah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 8 No 02 (2015)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol8.no02.a314

Abstract

The aim of study to test the inhibitory solution of lidah merua (Sansevieria trifasciata) on amount of bacteri, protein content, and organoleptic characteristics (color, aroma, and, mucus) of broiler chickens meat (Gallus domesticus). Study was conducted in a completely randomized experimental design with 5 level solution of S. Trifasciata (0, 15, 30, 45, and 60 g/100 ml), each of treatment was 4 replication. Application have done by soaking 100 g broiler chickens meat in solution of S. Trifasciata for 30 minutes. The result of this study shown soaking broiler chickens meat in solution of S. Trifasciata significant (P<0,05) influence amount of bacteri, but not significant (P> 0.05) influence protein content, and organoleptic characteristics (color, aroma, and mucus). solution of S. Trifasciata concentrations 15 g/100 ml can be durable chicken brioler meat and not change protein content. But organoleptic characteristics color and aroma of broiler chickens meat which like panelists are the concentration 15 g/100 ml and mucus of broiler chickens meat which like panelists are the concentration 45 g/100 ml. Study conclude that soaking broiler chickens meat in solution of S. Trifasciata can be hamper amount of bacteri but not change protein content and organoleptic characteristics (color, aroma, and mucus) of broiler chickens meat. Keywords: Sansevieria trifasciata broiler chickens meat amount of bacteri protein content organoleptic