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Jurnal Kependudukan Indonesia Vol 14, No 2 (2019)
Publisher : Research Centre for Population, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jki.v14i2.376


One of the tasks of  Kampung KB (Keluarga Berencana) is to reduce the proportion of unmet need for family planning by utilizing family planning field officers and gaining support from related institutions. This study aims to analyze the process of handling the unmet need for family planning in two Kampung KB in Cianjur Regency. The research used a qualitative approach in forms of individual in-depth interviews and focus group discussions. Data are analyzed descriptively based on case studies. The findings indicate that data recording instruments used daily in the research sites have not been able to identify groups of women with unmet need for family planning. Therefore, the existing services of family planning information have not been based on the needs of the target group. After the formation of Kampung KB, family planning information services and contraception services were more vigorous. While all of the services ease couples of childbearing age with unmet need for family planning to get related information and contraception, monitoring and evaluation activities still cannot be carried out because of the unavailability of officers as well as the target group and post-service data. In addition, there is no clear division of labor between the board Kampung KB and other family planning field officers in each stage of the handling of unmet need for family planning.
Jurnal Biometrika dan Kependudukan Vol 10, No 1 (2021): JURNAL BIOMETRIKA DAN KEPENDUDUKAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbk.v10i1.2021.94-104


This analysis aimed to examine the relationship between family development and risky adolescent sexual behavior in Indonesia. The data were taken from the 2018 Performance and Accountability Survey with a family and adolescent questionnaire; thus, the relationship between parents and adolescents could be identified. The statistical analysis methods used were descriptive and inferential analyses, with the unit of analysis being 15,556 teenagers who dated. The results of the logistic regression analysis (Model 1) by using all the independent variables simultaneously found that sexual risk behavior was mostly found in in boys, age categories 20-24 years, and participants who never had access to PIK-R. Risky sexual behavior was also dependent on whether participants agreed to have relations sexual before marriage, and also the level of education of their parents. The logistic regression analysis (Model 2) found residence, gender, level of adolescent education, age categories, agreement to have sexual relations before marriage, age groups of head of family, gender of head of family, education level of head of family, work status of head of family, economic status, and the activeness of the BKR activities contributed to adolescents committing risky sexual behaviors.
The Effectiveness of Mugwort Leaf Extract and Gotu Kola Leaf Extract against Acne Bacterial Activity Sari, Diah Puspita; Yuniar, Shintya; Fadillah, Siti Awalia Nur; Mutiarani, Afifi; Kusumawaty, Diah
ASEAN Journal of Science and Engineering Vol 2, No 3 (2022): AJSE: Volume 2, Issue 3, 2022
Publisher : Universitas Pendidikan Indonesia (UPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (835.089 KB) | DOI: 10.17509/ajse.v2i3.39634


Acne is a problem among teenagers generally caused by bacterial infections such as Propionibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis. Mugwort (Artemisia vulgaris L.) and Gotu Kola (Centella asiatica L.) extracts have antibacterial activity formulated in anti-acne gel preparations. The purpose of this study was to test and obtain a gel formula of mugwort leaf extract and gotu kola leaf extract which effectively kills and controls the growth of acne-causing bacteria. The methods used in this study include simplicity making, leaf extraction, making anti-acne gel preparations, testing pH, homogeneity, inhibition, and data analysis. The extract obtained by maceration was made in three concentrations, namely 2.50; 5.00; and 10.00%. The results showed that the pH value of the gel preparations obtained was in the range of 8.08-9.96. Mugwort leaf extract, gotu kola leaf, and mugwort-gotu kola have a distinctive smells with a thick texture at a concentration of 2.50% and a liquid texture at a concentration of 10.00%. Inhibition tests showed that the mugwort extract sample had a value of 16-18 mm, while the gotu kola extract and mugwort-gotu kola had a value of 14 mm. Mugwort leaf extract is able to inhibit the growth of acne-causing bacteria. This success was obtained because the extracts of mugwort and gotu kola leaves contain flavonoids that can inhibit the growth of bacteria that cause acne. Mugwort leaf extract gel with a concentration of 2.50 and 5.00% had the largest zone of inhibition in inhibiting P. acnes bacteria compared to other concentrations.
Penanda DNA: Uji Halal pada Makanan Olahan Daging Menggunakan Primer Multiplex PCR (Polymerase Chain Reaction) Dzikrina, Hanina; Sari, Diah Puspita; Faridah, Nurul; Saidah, Salsabila Shafa; Nur Alifah, Salma Annisa; Kusumawaty, Diah
JURNAL BIOS LOGOS Vol 12, No 1 (2022): Jurnal Bios Logos
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v12i1.36437


Multiplex-PCR (mPCR) is a technique that is gaining popularity due to its ability to detect multiple species in a single tube, resulting in faster results with more efficient and cost-effective resources than the conventional PCR method. This study aimed to design mPCR genetic markers for the detection of bovine/rat/chicken/pork organisms in processed foods. The procedure was divided into two stages: in silico and in vitro. The primers were designed based on mitochondrial cytochrome genes obtained from the NCBI GeneBank. Following alignment, the selected DNA fragments were used as the target sequence for the primer design. The sequences of all primer pairs were aligned on the Oli2go primary pooler to determine whether there was a possibility of cross reaction. The results of this study indicated that primers designed for the COX1 gene in rats and bovine, the KEF22 r01 gene in pork, and the ND6 gene in chicken produced amplicons with sizes of 622 bp, 552 bp, 588 bp, and 272 bp, respectively.  However, the amplicons generated from pork, bovine and mouse marker primers were difficult to distinguish when electrophoresed in the same 2% agarose gel. Meanwhile, the amplicons of the primer markers of chickens can be distinguished when electrophoresed with pork/cows/rat. The study concludes that the designed primer pairs can be used together in mPCR if the difference in amplicon size is greater than 200 bp.Key words: Multiplex PCR; DNA markers; Halal meat test.ABSTRAKMultiplex-PCR merupakan metode yang saat ini sedang populer untuk digunakan karena dapat melakukan pendeteksian lebih dari satu spesies dalam satu tabung, sehingga hasil yang diperoleh lebih cepat, efisien, dan murah dibanding metode PCR biasa. Penelitian ini bertujuan untuk merancang penanda genetik multipleks-PCR untuk deteksi adanya organisme sapi/tikus/ayam/babi pada makanan olahan. Metode yang digunakan terdiri dari dua tahap, yaitu secara in silico dan in vitro. Primer dirancang dengan menggunakan gen sitokrom mitokondria yang didapat dari GeneBank NCBI. Setelah dilakukan alignment, fragmen DNA yang terpilih dijadikan sebagai target sekuen untuk desain primer. Semua sikuen pasangan primer di-alignment pada Oli2go primer pooler untuk melihat kemungkinan adanya cross reaction.  Hasil dari penelitian ini yaitu primer tikus dan sapi dirancang dengan menggunakan gen COX1, primer babi menggunakan gen KEF22_r01, dan primer ayam menggunakan gen ND6 menghasilkan amplikon dengan ukuran berturut-turut 622 pb, 552 pb, 588 pb dan 272 pb. Namun, amplikon yang dihasilkan dari primer penanda babi, sapi dan tikus sulit dibedakan saat dielektroforesis dalam sumur gel agaros 2% yang sama. Sedangkan amplikon dari pasangan primer penanda ayam dapat terbedakan saat dielektroforesis baik bersama babi/sapi/tikus. Kesimpulan dari penelitian ini adalah hasil rancangan primer penanda telah dapat digunakan secara bersama jika perbedaan ukuran amplikon >200 pb.Kata kunci: Multiplex PCR; Penanda DNA; Uji halal daging.