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All Journal Bioscientia Medicina : Journal of Biomedicine and Translational Research Bioscientia Medicina : Journal of Biomedicine and Translational Research
Sahidan Sahidan
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

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Evaluation of Malondialdehyde in Type 2 Diabetes Mellitus Patients as Oxidative Stress Markers in Bengkulu Population Raden Sunita; Sahidan Sahidan; Rachmat Hidayat
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 4 No. 3 (2020): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v4i3.146

Abstract

Hyperglycemia in Type 2 Diabetes Mellitus (T2DM) can cause individuals to experience oxidative stress conditions, accompanied by a decrease in antioxidant activity in protecting cellular components against the attack of reactive oxygen species (ROS). ROS oxidized lipid components can produce Malondialdehyde (MDA). Evaluation of MDA can be used as a marker Oxidative stress which can indirectly describe the effects of ROS because ROS compounds are reactive. This study aims to determine the analysis of MDA in T2DM subjects at the Sentinel Public Health Center in Bengkulu City in 2017.research Analytic with a case control study design in 40 samples of T2DM subjects (cases) and 40 Non-DM samples (control) were carried out at the Sentinel DM Health Center in Bengkulu City. Data collection used questionnaires, measurements of Body Mass Index, examination of Fasting Blood Glucose and plasma MDA (spectrophotometry). The results of this study there were differences in characteristics (Gender, Age, BMI, Blood Pressure, Fasting Blood Glucose) in subjects with T2DM and Non-T2DM in the Bengkulu City Sentinel Health Center in 2017 (p <0,000). There was a significant difference (p= 0,000) in higher MDA levels in patients with T2DM than non-DM subjects. The MDA frequency distribution in the case was MDNormal (24%) and MDA Normal (6%), whereas in the control was MDA Not Normal (26%) and MDA Normal (44%). There were significant differences in MDA frequency distribution between cases and controls (p= 0,000). The subjects of T2DM had a risk of 6.77 times an increase in MDA levels compared to Non-DMT2 (p= 0.000, OR 6.77 IK 95% 2.44-18.73). The correlation between levels of GDP and MDA was statistically significant (p <0.001) with a positive correlation direction (0.340). The results of this study are expected to be used as a basis for the development of individual therapy in subjects with T2DM in order to prevent the occurrence of complications early on.
Evaluation of Malondialdehyde in Type 2 Diabetes Mellitus Patients as Oxidative Stress Markers in Bengkulu Population Raden Sunita; Sahidan Sahidan; Rachmat Hidayat
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 4 No. 3 (2020): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v4i3.146

Abstract

Hyperglycemia in Type 2 Diabetes Mellitus (T2DM) can cause individuals to experience oxidative stress conditions, accompanied by a decrease in antioxidant activity in protecting cellular components against the attack of reactive oxygen species (ROS). ROS oxidized lipid components can produce Malondialdehyde (MDA). Evaluation of MDA can be used as a marker Oxidative stress which can indirectly describe the effects of ROS because ROS compounds are reactive. This study aims to determine the analysis of MDA in T2DM subjects at the Sentinel Public Health Center in Bengkulu City in 2017.research Analytic with a case control study design in 40 samples of T2DM subjects (cases) and 40 Non-DM samples (control) were carried out at the Sentinel DM Health Center in Bengkulu City. Data collection used questionnaires, measurements of Body Mass Index, examination of Fasting Blood Glucose and plasma MDA (spectrophotometry). The results of this study there were differences in characteristics (Gender, Age, BMI, Blood Pressure, Fasting Blood Glucose) in subjects with T2DM and Non-T2DM in the Bengkulu City Sentinel Health Center in 2017 (p <0,000). There was a significant difference (p= 0,000) in higher MDA levels in patients with T2DM than non-DM subjects. The MDA frequency distribution in the case was MDNormal (24%) and MDA Normal (6%), whereas in the control was MDA Not Normal (26%) and MDA Normal (44%). There were significant differences in MDA frequency distribution between cases and controls (p= 0,000). The subjects of T2DM had a risk of 6.77 times an increase in MDA levels compared to Non-DMT2 (p= 0.000, OR 6.77 IK 95% 2.44-18.73). The correlation between levels of GDP and MDA was statistically significant (p <0.001) with a positive correlation direction (0.340). The results of this study are expected to be used as a basis for the development of individual therapy in subjects with T2DM in order to prevent the occurrence of complications early on.
Polymorphism E23K KCNJ11 Gen as a Risk Factor of Diabetes Mellitus in Serawai Tribe Of Bengkulu Raden Sunita; Sahidan Sahidan; Rachmat Hidayat; Resva Meinisasti
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 4 No. 1 (2020): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v4i1.105

Abstract

ABSTRACT Background: Type 2 diabetes mellitus (T2DM) is a multifactorial disease involving genetic and environmental factors. The E23K KCNJ11 gene polymorphism causes KATP canal overactivity, decreases cell membrane depolarization potential, and decreases insulin secretion. E23K polymorphism of the KCNJ11 gene as a risk factor for T2DM. Research Objective: This study aimed to analyze the E23K polymorphism of the KCNJ11 gene as a risk factor for T2DM in the Bengkulu Serawai. Method: This study is a case-control study. The subjects of the study were 100 people with T2DM patients as a case group (50 people) and Non-DM subjects with families who did not have a history of T2DM as a control group (50 people). Fasting blood glucose (GDP) was analyzed by spectrophotometry and E23K KCNJ11 gene by polymerase chain reaction fragment length polymorphism (PCR-RFLP). Data were analyzed by statistics. Results: The frequency of AA genotypes in cases was higher than the controls (12% and 8%) (p = 0.001). The frequency of A allele in the case was higher than the control (32% and 18%) (p = 0.017). The risk of T2DM on AA / GA genotypes was 4.75 times higher in cases than controls (p = 0,000, OR 4.75 95% CI 2.01-11.24). The risk of T2DM in A allele was 2.14 times higher in cases than in controls (p = 0.017, OR 2.14, 95% CI 1.11-4.15). Conclusion: E23K polymorphism of the KCNJ11 gene as a risk factor for T2DM in Bengkulu Serawai Tribe. Keywords: E23K gene KCNJ11, DMT2, Non-DMT2.
Polymorphism E23K KCNJ11 Gen as a Risk Factor of Diabetes Mellitus in Serawai Tribe Of Bengkulu Raden Sunita; Sahidan Sahidan; Rachmat Hidayat; Resva Meinisasti
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 4 No. 1 (2020): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v4i1.105

Abstract

ABSTRACT Background: Type 2 diabetes mellitus (T2DM) is a multifactorial disease involving genetic and environmental factors. The E23K KCNJ11 gene polymorphism causes KATP canal overactivity, decreases cell membrane depolarization potential, and decreases insulin secretion. E23K polymorphism of the KCNJ11 gene as a risk factor for T2DM. Research Objective: This study aimed to analyze the E23K polymorphism of the KCNJ11 gene as a risk factor for T2DM in the Bengkulu Serawai. Method: This study is a case-control study. The subjects of the study were 100 people with T2DM patients as a case group (50 people) and Non-DM subjects with families who did not have a history of T2DM as a control group (50 people). Fasting blood glucose (GDP) was analyzed by spectrophotometry and E23K KCNJ11 gene by polymerase chain reaction fragment length polymorphism (PCR-RFLP). Data were analyzed by statistics. Results: The frequency of AA genotypes in cases was higher than the controls (12% and 8%) (p = 0.001). The frequency of A allele in the case was higher than the control (32% and 18%) (p = 0.017). The risk of T2DM on AA / GA genotypes was 4.75 times higher in cases than controls (p = 0,000, OR 4.75 95% CI 2.01-11.24). The risk of T2DM in A allele was 2.14 times higher in cases than in controls (p = 0.017, OR 2.14, 95% CI 1.11-4.15). Conclusion: E23K polymorphism of the KCNJ11 gene as a risk factor for T2DM in Bengkulu Serawai Tribe. Keywords: E23K gene KCNJ11, DMT2, Non-DMT2.
Co-Authors RACHMAT HIDAYAT Raden Sunita Resva Meinisasti, Resva