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Characterization of Ethanolic Extract of Streptomyces sp. as a Pancreatic Lipase Inhibitors Produced by Endophytic Streptomyces sp. AEBg12 Fitri, Lenni; Meryandini, Anja; Iswantini, Dyah; Lestari, Yulin
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8907

Abstract

Endophytic Streptomyces sp. AEBg12 isolated from Zingiber cassumunar (Bangle) is known to produce pancreatic lipase inhibitory compound. However, the characteristics of this active compound has not been reported yet. This study aimed to determine the characteristics of pancreatics inhibitory compound produced by Streptomyces sp. AEBg12 and to assess the role of endophytic actinobacteria in producing pancreatic lipase inhibitor using endophytic-free bangle tissue culture, wild bangle and compared with the activity of Streptomyces sp. AEBg12 endophytes. Supernatant of Streptomyces sp. AEBg12 was extracted using ethanol, ethyl acetate, and n-hexane solvents. Toxicity test was performed using larvae of shrimp Artemia salina. The results showed that the best solvent to obtain pancreatic lipase inhibitor compounds was ethanol. Phytochemical analysis showed that ethanolic extract of endophytic Streptomyces sp. AEBg12 contained flavonoids. IC50 value of ethanol extract was 180.83 g/ml. The result of TLC showed that ethanolic extract of Streptomyces AEBg12 had a blue luminescence band indicated that there were either flavone, flavanones, flavonols or isoflavones. Inhibitory activity of Streptomyces sp. AEBg12 was higher than wild bangle and bangle tissue culture. The information from this study can be be used as a basic data for further characterization of the active compound, which might be developed as an antiobesity agent through its pancreatic lipase inhibitory activity.
The Potential Endophytic Bacteria Isolated from Rice (Oryza sativa) as Biofertilizer Putriani, Putriani; Fitri, Lenni; Ismail, Yulia Sari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.252 KB) | DOI: 10.15294/biosaintifika.v11i2.18401

Abstract

Endophytic bacteria are beneficial microorganisms that interact with host plants without causing any interference or damage to plants. This research aimed to obtain endophytic bacteria isolated from the root tissue of rice plants (Oryza sativa L.) which have potential to produce IAA hormones,, identify the endophytic bacteria in morphologically and physiologically, and analysis of the selected isolate 16S rRNA genes. Based on the results of this research, there  were six endophytic bacteria isolates obtained. They have high morphological diversity and differen ability producing IAA hormones. The highest concentration of IAA (425 ppm) was obtained  isolates from  EAP3. Isolate EAP3 also produce inhibit the growth of Xanthomonas oryzae with a 5.2 mm inhibition zone. Based on the biochemical test, EAP3 had 60% similarity with Enterobacter asburiae. Analysis of the 16S rRNA gene showed that EAP3 had the highest similarity with Enterobacter asburiae strain U4 by 99%. This research data is considered as new information about the potential of endophytic bacteria from the roots of rice plants (Oryza sativa L.) which is capable of producing IAA hormones and is able to inhibit pathogenic bacteria. This research provides information that can be used as a basis for developing endophytic bacteria as biological fertilizers. 
Analisis Efektifitas Tepung Jamur sebagai Larvasida Aedes aegypti Yasmin, Yekki; Fitri, Lenni; Bustam, Betty Mauliya
Jurnal Natur Indonesia Vol 14, No 1 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.759 KB) | DOI: 10.31258/jnat.14.1.126-130

Abstract

This research is about effectivity analysis of two kinds of fungi i.e. Beauveria bassiana and Metharrizium anisopliae as Aedes aegyptilarvacide, it has been conducted at laboratories Zoology in Biology Department, Mathematics and Natural Sciences Faculty, Syiah KualaUniversity. Aims of this research were estimating maximum time storage of fungi powder as effective larvacide and the number of bacteriacolonies were found in water used for experiment. This research was used Completely Randomized Design (CRD). The experiments consistof two factors i.e kind and saving time of fungi. The result show that the longer the storage time of Beauveria bassiana the more ineffectiveit was as larvacide and the least fungi colonies were found. Metharrizium anisopliae on the other hand, the longer it was kept, the moreeffective it was as larvacide and the more fungi colonies were found.
APPLICATION OF RICE ROOT ENDOPHYTIC BACTERIA IN CIHERANG VARIETY RICE (ORYZA SATIVA) SEEDS Fitri, Lenni; Ismail, Yulia Sari; Putriani, Putriani; Warzatullisna, Warzatullisna
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.21697

Abstract

Endophytic bacteria are microorganisms that thrive in plant tissue without causing any negative effects to the host plants. The purpose of this study was to apply endophytic bacteria that have the potential to produce IAA hormones as biological agents in rice seeds. This research was conducted by testing IAA hormone using a spectrophotometer, testing HCN production using filter paper, biochemically identification of the isolate, and applicating endophytic bacteria into rice seeds. Endophytic bacteria have different abilities in producing IAA hormone. Based on the results of the study, the highest IAA concentration (57 ppm) was obtained from KSB1 (Potassium Solubilizing Bacteria) isolates and followed by KSB3 isolate with 56 ppm. All the endophytic bacteria isolates were unable to produce HCN. Results of the study showed that KSB1 and KSB3 isolate were able to influence the growth of root length by 5.62 cm and 6.70 respectively as well as the canopy height by 8.56 cm and 8.30 cm respectively. Based on observations of colony morphology, cell morphology and biochemical tests, KSB1 was presumably belong to the genus Staphylococcus. In this study, new information about endophytic bacteria that can increase the growth of Ciherang variety rice can be obtained. This study provides information that the application of environmentally friendly bacteria is important to preserve the environment and agriculture sustainability in Indonesia without reducing the productivity of rice.
ISOLASI DAN PENGAMATAN MORFOLOGI KOLONI BAKTERI KITINOLITIK Fitri, Lenni; Yasmin, Yekki
Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi Vol 3, No 2 (2011): Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.106 KB)

Abstract

The research is aimed to isolate and observe the morphology of chitinolitic bakteria. The research was conducted since November 2010 until Apryl 2011 at Microbiology Laboratory ofMathematics and Sciences Faculty, University of Syiah Kuala. The method used is experimental method. Locations of water sample taken are at sea, river, and embankment which taken in some areas around Banda Aceh and Aceh Besar. The results showed that there are 18 pure cultuated chitinolitic bacteria isolates. And the results of morphologycal bakteria colony showed that all isolates were circle shape colony. It was found that the flat shape of colony is 14 isolates and 4 serrated. The sizes of colony were found from 1,0 mm until 3,5 mm. The white isolate are 13 and 5 yellowish. The most of isolates are negative gram which bacil and coccus cell shape.
Isolation of Potential Plant Growth Promoting Rhizobacteria (PGPR) from Cassava (Manihot esculenta) Rhizosphere Soil Safriani, Suri Raihan; Fitri, Lenni; Ismail, Yulia Sari
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25905

Abstract

Cassava is the third most important calorie source in tropical area after rice and corn. The microorganism associated with cassava roots may be potent and useful for application to promote plant growth. Plant growth promoting rhizobacteria  is a group of benefical bacteria that live in rhizosphere. The aim of this study was to isolate and to identify the potential Plant Growth Promoting Rhizobacteria (PGPR) from cassava rhizosphere soil. The study consisted of isolation and identification of bacteria based on morphological and biochemical characters, hypersensitive reaction test, the ability to solubilize potassium and phosphate, and the ability to inhibit the growth of pathogen Sclerotium rolfsii. A total of nine bacteria isolates were succesfully isolated from Cassava rhizosphere soil. Those isolates suspected as Micrococcus sp.1, Micrococcus sp.2, Micrococcus sp.3, Micrococcus sp.4, Micrococcus sp.5, Micrococcus sp.6, Neisseria sp.1, Neisseria sp.2 and Bacillus sp. All nine isolates did not show hypersensitivity reactions. Only Neisseria sp.1 and Neisseria sp. 2 were able to solubilize potassium and phosphate. All isolates were able to inhibit the growth of S. rolfsii. The highest inhibition was done by Micrococcus sp.4 (51.46 %). The physical and chemical properties of cassava rhizosphere soil affected the type of bacteria found in this study. The result confirmed that the potential isolates obtained from cassava rhizosphere soil can be plant growth promoters. The present study suggested that PGPR isolates might have potential in future field applications as plant growth promoters or biocontrol agents.
Exploration of IAA and HCN Producing Rhizobacteria from Cassava (Manihot esculenta Crantz) Rhizosphere Safriani, Suri Raihan; Fitri, Lenni; Ismail, Yulia Sari
Jurnal AgroBiogen Vol 16, No 2 (2020): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n2.2020.p71-78

Abstract

Plant growth promoting rhizobacteria (PGPR) are a group of beneficial bacteria that live in rhizosphere. These bacteria can promote plant growth through several mechanisms, such as the ones produce indole-3-acetic acid (IAA) hormone and hydrogen cyanide (HCN), and act as biocontrol agents. The use of PGPR to promote plant growth has been known to be an environmentally friendly alternative approach. The aim of this study was to explore IAA and HCN producing rhizobacteria from cassava rhizosphere soil and identify the bacteria based on morphological and biochemical characters, hypersensitive reaction test, and the ability test to produce IAA and HCN. The results showed nine bacterial isolates suspected as Micrococcus sp. (six isolates), Neisseria sp. (two isolates), and Bacillus sp. (one isolate). All isolates were able to produce IAA in the concentration range of 50,63–135,00 µg/ml and 232,3–333,9 µg/ml at incubation time of 2 and 4 days, respectively. All isolates were able to produce HCN. In addition, the isolates did not show hypersensitivity reactions. Further study is needed to assess the isolate application for promoting plant growth as well as a biocontrol agent of plant pathogen.
Potensi antimikroba aktinobakteri endofit daun sirih (Piper betle L) Lenni Fitri
Jurnal Bioleuser Vol 2, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, University of Syiah Ku

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (912.633 KB) | DOI: 10.24815/jobioleuser.v2i1.11998

Abstract

Mikroba endofit adalah mikroba yang hidup pada jaringan tanaman tanpa menimbulkan gejala penyakit pada tanaman inangnya. Mikroba endofit diharapkan mampu menghasilkan metabolit sekunder yang sama dengan tanaman inangnya. Penelitian ini bertujuan untuk mengisolasi aktinobakteri endofit dari daun tanaman sirih dan menguji aktivitasnya sebagai antimikroba. Isolasi aktinobakteri endofit menggunakan media agar humid acid vitamin. Keragaman morfologi aktinobakteri endofit diobservasi berdasarkan pengamatan langsung koloni aktinobakteri endofit yang ditumbuhkan pada media ISP2 dan observasi morfologi secara mikroskopis. Dari hasil isolasi ditemukan 16 isolat aktinobakteri endofit. Dari hasil uji terhadap bakteri didapatkan bahwa zona hambat paling besar didapatkan pada isolat DS 7 dan DS 13   (14 mm). Isolat  DS 10 mampu menghambat Fusarium oxysporum dengan zona hambat yang terbentuk sekitar 32 mm.
Kemampuan Daya Hambat Beberapa Macam Sabun Antiseptik Terhadap Pertumbuhan Staphylococcus aureus dan Escherichia coli Lenni Fitri
Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi Vol 2, No 2 (2010): Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.803 KB)

Abstract

This research is  aimed to find out ability  some kind antiseptic soaps inhibiting the growth of Staphylococcus aureus and Eschericia coli bacteria. The research method used Randomized Completely Block Design Sub Sampling with 6 treament and 3 replication, they are: antiseptic soap A, B, C, D, phenol 2% and aquadest. The group consist of S. aureus and E. coli bacteria. The inhibit experiment was done by using Kirby-Bauer methode. The parameter analyzed by using Analysis of Variance (ANOVA) continue with the Duncan’s Multiple Range Test (DMRT). The result showed that there were  significant differences of inhibit zone that formed from both bacterias . The result of the antiseptic soap suspend inhibit zone, S. aureus bacteria has larger diameter than E. coli. Soap A, B, C and D with 50% concentrate toward both bacteria, each has average diameter 25, 16 mm, 15,6 mm, 11,5 mm and 9,83 mm while phenol  has 15,0 mm. This result showed that soap A, B, and C has the equal ability to phenol 2%. Base on Duncan Multiple Range Test (DMRT), it is found out that the real differences of the inhibit zone between the two bacteria as well the real differences of treatment forward those bacteria (P0,05).
POTENSI BAKTERI KITINOLITIK SUMBER AIR PANAS SEBAGAI PENGENDALI HAYATI LARVA Aedes aegypti L. Fitria Ardani; Yekki Yasmin; Lenni Fitri
Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi Vol 4, No 2 (2012): Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.406 KB)

Abstract

The purpose of this study was to discover the potential of isolate chitinolitic bacteria, hot springs, as biological control of Aedes aegypti L. larva. This research used Completed Randomized Designed (RAL) factorial with two factor, isolate types and concentration of isolatechitinolitic bacteria. The research procedure includes the maintenances of Aedes aegypti L. larva, isolation and selection of chitinolitic bacteria, and biological test. Data were processed by using Varian Analysis (ANAVA). The result of this study indicated that four isolates were test on larva of Aedes aegypti L. masquito, instar II. Giving different isolat chitinolitic bacteria and concentration showed no significant differences in larva death (P0,05). The highest average of larva death was exhibited at the treatments of 1% of IS 2 and IS 3 isolate. The occurance of chitinolitic bacteria influenced the growth of pupa, it can be showed that the pupa was not formed from each consentration and isolate that caused the death in larva signed by the part of body and larva siphon were pale and digestive tract was dark.