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Hubungan antara Ekspresi Ki-67 dengan Stadium Klinis dan Derajat Histopatologis Karsinoma Sel Skuamosa Serviks Nita Hertati; Heni Maulani; Zulkarnain Musa; Zen Hafy
Majalah Patologi Indonesia Vol 23 No 3 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Abstract

Background Cervical cancer is the third most common type and the fourth most deadly cancerian women which remains a significant health problem worldwide especially in developing coumtry. Ki-67 is a prototype protein in nuclear which influence cell proliferation rate, hence it can be used as one of parameter to evaluate progressivity of tumor cells in carcinoma cases as the cervical squamous cell carcinoma (SCC). This study aims to determine the relationship between clinical staging of cervical SCC and histopatological grading of the cervical SCC with the expression of Ki-67. Methods This was an observational study with a cross sectional design. Sixty cervical squamousa cell carcinoma archive histopathological slide samples (45 biopsy specimens and 15 radical hysterectomy tissues) were collected for this study from the Anatomical Pathology Department of RSUP dr. Mohammad Hoesin Palembang (January 2008 to July 2013). The samples were stained with Ki-67 antibody and analyzed with respect to patient’s clinical stage and histopatological grade. Results The positivity of Ki-67 expressions were more common in the age of ≤ 50 year-old group, in the non-keratinizing type, in the late clinical staging (stage IIIB) and in the moderatelly differentiated histopathological grade. High scores of the Ki-67 expressions (Ki-67>20%) was predominantly found in the late clinical stage and moderatelly differentiated histopathological grade cases. Statistically, there was a significant relationship (p=0.034) with a strong correlation (r=0.714) between the Ki-67 ecpression and the clinical stage of cervical SCC, but not histopathological grade. Conclusion The higher clinical staging of cervical SCC, the higher expression of Ki-67. Key words: cervical squamous cell carcinoma, clinical staging, histophatological grade, Ki-67.
Ekspresi Epidermal Growth Factor Receptor dan Karakteristik Klinikohistopatologik pada Karsinoma Kolorektal . Reallyani; Mezfi Unita; Zulkarnain Musa; Syarif Husin
Majalah Patologi Indonesia Vol 23 No 1 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Background Colorectal carcinoma is one of the world's fourth most malignancies. Frequency of colorectal carcinoma malignancy most likely to occur in the gastrointestinal tract and causes the death of close to 15% of all cancers. Heterogeneous of the etiology is associated with many risk factors and molecular changes. Epidermal growth factor receptor is a 170 kDa transmembrane glycoprotein which contribute to carcinogenesis, prognostic impact and specific therapeutic targets. The aims this study to determine the relationship between EGFR expression and the clinicohistopathology characteristics in colorectal carcinoma. Methods This study is an observational study with cross sectional approach. The samples were specimens of the 57 samples that had been diagnosed as colorectal carcinoma from January 2010 to August 2012. Samples from histopathological preparations are reviewed and conducted outward EGFR immunohistochemistry with primary antibodies for the primary tumor. Results EGFR expression to histopathological characteristics are as follows pT3 of 45.6%, positive lymph node status was 52.6% and poorly differentiated histological degree of malignancy of 50.9%. Statistical calculations showed no significant correlation between EGFR expression and histopathologic characteristics of the pT in the TNM system (p=0.037), lymph node status (p=0.000), histological grading of malignancy (p=0.001), and high expression (61.4%). Conclusion EGFR expression affects the pT in TNM system, lymph node staging and histological grading of malignancy. Key words: characteristic clinicohistopathology, colorectal carcinoma, epidermal growth factor receptor.
Korelasi Antara Overekspresi p53 Dengan Derajat Histopatologi Dan Stadium Klinis Karsinoma Ovarium Tati Lahmuddin; Heni Maulani; Zulkarnain Musa; Irsan Saleh
JURNAL KEDOKTERAN DAN KESEHATAN Vol 2, No 3 (2015)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

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Abstract

Karsinoma ovarium adalah keganasan yang berasal dari sel-sel epitelial di ovarium, terbanyak keenam pada wanita di dunia. Di RS Umum Dr. Mohammad Hoesin Palembang, insiden karsinoma ovarium menempati urutan ketiga keganasan terbanyak pada wanita setelah karsinoma payudara dan leher rahim. Gen TP53 mensintesis protein p53 yang bila inaktif dapat mempengaruhi derajat histopatologi dan stadium klinik. Penelitian ini bertujuan mengetahui korelasi antara overekspresi p53 dengan derajat histopatologi dan stadium klinik karsinoma ovarium.  Penelitian dilakukan secara analitik observasional, pendekatan potong lintang di sentra diagnostik laboratorium Patologi Anatomi RS Umum Dr. Mohammad Hoesin Palembang. Sampel berjumlah 32 kasus, diambil dari arsip rekam medik periode 1 Januari 2012 sampai 30 Juni 2015, dipulas dengan antibodi monoklonal p53. Hasil penelitian menemukan frekuensi karsinoma ovarium derajat rendah sebanyak 65,6% dan derajat tinggi sebanyak 34,4%. Tipe histopatologi terbanyak adalah karsinoma serosa derajat tinggi (25%) dan karsinoma musinus (25%). Frekuensi karsinoma ovarium stadium lanjut (53,1%) lebih banyak dari stadium awal (46,9%).  Frekuensi overekspresi p53 pada karsinoma ovarium 43,8%. Tidak ada korelasi bermakna antara overekspresi p53 dengan derajat histopatologi (p=0,1) dan stadium klinis karsinoma ovarium (p=0,6). Terdapat korelasi bermakna antara derajat histopatologi dan stadium klinik (p=0,00, r=0,46)
Ekspresi HPV16 Onkoprotein E7 pada Lesi Prakanker dan Karsinoma Sel Skuamosa Serviks Wresnindyatsih; Verli Saniba; Zulkarnain Musa; Zen Hafy
Majalah Patologi Indonesia Vol 29 No 2 (2020): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.581 KB) | DOI: 10.55816/mpi.v29i2.409

Abstract

BackgroundCervical cancer is the secondmost common malignancy in women worldwide.The vast majority of cervical cancersare associatedwith high risk HPV16 infection. The persistence of HPV16 and integration of viral DNA into cell genome are necessary for thedevelopment of precancerous lesion and squamous cell carcinoma (SCC) of the cervix. The integration of viral DNA in the host cellgenome resulted in the regulation of oncoprotein E7 leading to detection of HPV16 E7 expression in precancerous lesions and SCCof the cervix.The aim of this study was to evaluate differences of HPV16 E7 expression in precancerous lesions and cervical SCC.MethodsAn observational study with cross-sectional design was performedat Department of Anatomical Pathology Faculty of MedicineUniversity of Sriwijaya/dr. Mohammad Hoesin General Hospital Palembang, from 1st July 2014 to 30st Juni 2017. The selectedsamples consisted of 25 precancerous cases lesions (13 cases of LSIL and 12 samples of HSIL) and SCC (25 cases).Immuhistochemical stained was conducted using HPV16 E7 antibody. Fisher exact’s test was used to analyze differences inexpression of HPV16 E7 among precancerous lesions and cervical SCC.ResultsThe pracancerous lesions occurred mostly between the ages of 30-40 years, while SCC were those between the ages of 51-60years. All SCC cases (100%) and 91.6% of HSIL showed weak to strong HPV16 E7 expression, and 84.6% cases of LSIL showedimmunonegative.ConclusionThe positivity of HPV16 E7 increased in HSIL and KSS lesions.
Ekspresi E-cadherin dan p63 pada Karsinoma Sel Skuamosa Kulit dan Pseudoepitheliomatous Hyperplasia Amelia Amelia; ika Kartika; Zulkarnain Musa
Majalah Patologi Indonesia Vol 27 No 2 (2018): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Latar belakangPada spesimen biopsi yang berukuran kecil, terkadang patolog mengalami kesulitan untuk membedakan karsinoma sel skuamosa kulit dan pseudoepitheliomatous hyperplasia. Penurunan ekspresi E-cadherin dan overekspresi p63 dapat dijumpai pada neoplasia ganas, dan sebaliknya pada lesi non neoplastik. Tujuan penelitian ini untuk menganalisis perbedaan ekspresi E-cadherin dan p63 pada karsinoma sel skuamosa kulit dan pseudoepitheliomatous hyperplasia.MetodePenelitian ini merupakan penelitian observasional analitik dengan rancangan potong lintang. Spesimen karsinoma sel skuamosa kulit yang diteliti sebanyak 23 kasus (13 kasus dengan derajat diferensiasi baik, 6 kasus dengan derajat diferensiasi sedang, 4 kasus dengan derajat diferensiasi buruk) dan 15 kasus pseudoepitheliomatous hyperplasia, kemudian dilakukan pewarnaan imunohistokimia E-cadherin dan p63 dan dinilai ekspresinya.HasilPada karsinoma sel skuamosa kulit tampak penurunan ekspresi E-cadherin (p=0,000) dan overekspresi p63 (p=0,000) jika dibandingkan dengan pseudoepitheliomatous hyperplasia.KesimpulanPenelitian ini menunjukkan perbedaan ekspresi E-cadherin dan p63 antara kasus karsinoma sel skuamosa kulit kulit dengan pseudoepitheliomatous hyperplasia. Sehingga E-cadherin dan p63 merupakan dua marker penting yang cukup andal untuk membedakan kasus karsinoma sel skuamosa kulit khususnya membedakan karsinoma sel skuamosa kulit berdiferensiasi baik dengan pseudoepitheliomatous hyperplasia.
Karakteristik Histopatologi Melanoma Maligna di Bagian Patologi Anatomi RSUP Dr. Moh. Hoesin Palembang Tahun 2009-2013 Nisrina Ariesta Syaputri; Zulkarnain Musa; Indri Seta Septadina
Biomedical Journal of Indonesia Vol. 4 No. 1 (2018): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

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Melanoma maligna merupakan tumor ganas yang berasal dari melanosit dengan gambaran berupa lesi kehitam-hitaman pada kulit. Melanoma maligna merupakan satu dari tiga jenis kanker kulit yang mempunyai insiden terendahnamun angka kematian yang disebabkannya cenderung lebih besar yaitu 75% akibat kanker kulit. Penelitian inibertujuan untuk mengetahui karakteristik histopatologi melanoma maligna pada sediaan di Bagian Patologi AnatomiRSUP Dr. Mohammad Hoesin Palembang pada tahun 2009-2013. Jenis penelitian yang digunakan adalah metodedeskriptif observasional dengan pendekatan cross sectional. Dari 29.175 rekam medik pasien di Bagian PatologiAnatomi RSMH selama 2009-2013, diperoleh 30 kasus melanoma maligna yang memenuhi kriteria inklusi. Angkakejadian melanoma maligna di Bagian Patologi Anatomi RSUP Dr. Mohammad Hoesin Palembang tahun 2009-2013adalah 0.103%. Melanoma maligna lebih banyak ditemukan pada perempuan (70%) daripada laki-laki (30%), danpaling sering terjadi pada kelompok usia 45-53 tahun dengan persentase 30%. Karakteristik histopatologi yang palingbanyak ditemukan dalam penelitian ini adalah subtipe nodular melanoma (100%). Angka kejadian melanoma malignadi Bagian Patologi Anatomi RSMH tahun 2009-2013 adalah 0,103%, dengan angka kejadian tertinggi pada tahun 2012yaitu 0.28%. Nodular melanoma merupakan subtipe yang paling banyak ditemukan.