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All Journal Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Journal of Stem Cell Research and Tissue Engineering Ovozoa: Journal of Animal Reproduction
ERMA SAFITRI
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Agriculture, Biological Sciences & Forestry Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Pengaruh injeksi nikotin terhadap motilitas, viabilitas, dan integritas membran spermatozoa mencit (Mus Musculus) Kautsar Pandu Pramudita; Widya Paramita Lokapirnasari; Suheni Susilowati; Hermin Ratnani; Erma Safitri; Epy Muhammad Luqman
Ovozoa : Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.77-81

Abstract

The process of spermatogenesis is sensitive to nicotine, a main component of cigarette smoke. This study aimed to observe the effect of nicotine injection on motility, viability, and membrane integrity of the spermatozoa of mice (Mus musculus). The mice were 10 weeks old, weighing about 20 grams, and in healthy and fertile conditions. Mice were adapted for 1 week by being given feed in the form of pellets and drinking water every day. Twenty of the mice were divided into four groups. Group K as a control was injected with 0.9% NaCl at 2.5 mg/Kg bw. Group P1 was injected with nicotine at 2.5 mg/Kg bw. P2 was injected with nicotine at 5 mg/Kg bw. Group P3 was injected with nicotine at 10 mg/Kg bw. The injection was carried out subcutaneously as much as 0.5 ml once a day for 35 days. Data analysis used the Analysis of Variances (ANOVA), followed by Duncan's Multiple Range Test. The results showed that the viability, motility, and membrane integrity of the spermatozoa decreased significantly (p <0.05) according to the increase in the dose of nicotine injected. It could be concluded that subcutaneous nicotine injection linearly decreased the motility, viability, and membrane integrity of the spermatozoa of mice.
The Role of adaptive MSCs as an Attempt Regeneration of Spermatogenesis Process Using by Hypoxia Precondition In Vitro erma safitri
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 2 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i2.22752

Abstract

The aim of this research was to obtain to get adaptive MSCs by an treatment in hypoxia precondition in viro culture. Ini this research, hypoxia precondition was to be given 3% O2 concentration and compared to those of normoxia culture in O2 > 20%. Results showed that under 3% O2 concentration, CD105+ and CD45- by flowcytometri, immunocytokimia and immunofluorescence didn’t experience of change (undifferentiated). Meanwhile under > 20% O2 concentration, cells have experienced of change (not undifferentiated again), that was indicated with CD105+ become decrease and CD45- increase by flowcytometri, immunocytokimia and immunofluorescence. Conclusion, in this research showed that hypoxia precondition with 3% O2 concentration very support MSCs to constantly adaptive before transplantated for disturbance of spermatogenesis process, because did’nt experience become progenitor cells was not expectation (still undifferentiated).
Co-Authors . Helen . Mulyani . Purwati Abdullah Abdullah Candra Bumi Dilla Aranda Mayola Dirga Januar Surya Utama Emy Koestanti Sabdoningrum Epy Muhammad Luqman Fedik Abdul Rantam Hana Eliyani Hani Plumeriastuti Hardijanto Hardijanto Hermin Ratnani Herry Agoes Hermadi Imam Mustofa Ismudiono Ismudiono Kautsar Pandu Pramudita Koesnoto Supranianondo Mas’ud Hariadi Nanda Irsa Meydilasari Nunuk Dyah Retno Lastuti R. Heru Prasetyo Rimayanti Rimayanti Rochmah Kurnijasanti Rosiana Febrianila Rudi Irvanto Sri Hidanah Sri Mulyati Sri Pantja Madyawati Sri Wigati Mardi Suheni Susilowati Suherni Susilowati Sunaryo Hadi Warsito Suzanita Utama Tantri Nawangwulan Tatik Hernawati Tita Damayanti Tita Damayanti L Tita Damayanti Lestari Tjuk Imam R. Tjuk Imam Restiadi Tri Nurhajati Trilas Sardjito Vina Ari Prastyorini Wahyu Retno Swari Widya Paramita Widya Paramita L. Widya Paramita Lokapirnasari wurlina wurlina