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Hydrolysis of Palm Kernel Cake (Elaeis guineensis Jacq) by Fungi Trichoderma reesei that Degrades Mannan Polysaccharides Jaelani, A; Piliang, WG; Suryahadi, Suryahadi; Rahayu, I
ANIMAL PRODUCTION Vol 10, No 1 (2008): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The research was conducted to study the growth characterization of Trichoderma reesei, the degradability of mannan polysaccharides from Palm Kernel Cake (PKC) with different concentrations of fungi and thickness of the PKC medium, as well as the improvement of the nutritive value of palm kernel cake. Experiment 1 was to study the growth characterization of Trichoderma reesei, and Experiment 2 was conducted using concentrations of fungi (104, 105, and 106 CFU per cc) and thickness of the PKC medium (1, 2, and 3 cm). The research used a completely randomized design with factorial 3 x 3. The treatments resulted in significant (P<0.05) differences on pH and temperature of PKC medium, ADF, NDF, crude protein, and hemicellulose.  There were interaction effect on the concentrations of fungi and on the thickness of PKC medium (P<0.05) on NDF, crude protein, and hemicelluloses (P<0.05). Experiment 3 was conducted to study the True Metabolizable Energy (TME), the retention of nitrogen, total sugar and mannan content. Results showed that Trichoderma reesei was able to degrade mannan polysaccharides of PKC whereby TME and the total sugar value increased, but the nitrogen retention and mannan content decreased. (Animal Production 10(1): 42-49 (2008) Key Words: Hydrolysis, palm kernel cake, Trichoderma reesei, mannan polysaccharides
Casrea Engineering Based on Extruded-Cassava-Urea as Protein Supplement for Slow Release of Ammonia in the Rumen In Vitro Prasetiyono, BWHE; Suryahadi, Suryahadi; Toharmat, T; Syarief, R
ANIMAL PRODUCTION Vol 10, No 1 (2008): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

CASREA is protein supplement (PS) based on extruded-cassava-urea as main materials.  The objective of this study was to examine the CASREA characteristics by evaluating ammonia concentration (NH3), Volatile fatty acids (VFA), rumen microbial protein synthesis (RMPS), and protein digestibility post rumen (PDPR). Variables of NH3, VFA and RMPS were analyzed with a completely randomized design in factorial (4X3), namely CASREA as factor A, consisted of: Casrea1 (made from 32% urea and 58% cassava without extrusion), Casrea2 (extrusion 22% urea and 68% cassava), Casrea3 (extrusion 27% urea and 63% cassava), and Casrea4 (extrusion 32% urea and 58% cassava), whereas the factor B was incubation times, consisted of  2, 4, and 6 hours incubation time. Variable of PDPR was analyzed with a completely randomized design, and the treatments were Casrea1, 2, 3, and 4. Results showed that there were no interactions between CASREA and incubation time on variables of NH3, VFA, and RMPS. Extrusion on CASREA materials reduced (P<0.05) NH3 and VFA, but increased (P<0.05) RMPS and PDPR.  The highest RMPS and PDPR were 29.04 mg and 76.16%, respectively and were attained by Casrea2.  Casrea1 had the highest NH3 (43.42 mM), whereas Casrea2 had the lowest (29.65 mM).  Meanwhile, NH3 caused by Casrea2, Casrea3, and Casrea4 was 29.65, 30.54 and 31.44 mM, respectively and the increase these values were not significantly different. The concentration of NH3, VFA, and RMPS were stable on 4 hours incubation time. The present findings suggest that CASREA made from extrusion of 22% urea and 68% cassava as main materials was PS for slow release of ammonia (SRA) in the rumen due to reduction of NH3, but it improved the utilization of nitrogen for rumen microbial protein synthesis.  (Animal Production 10(1): 34-41 (2008) Key Words: Extruded-cassava-urea, protein supplement, slow release of ammonia
Pembuatan Kartu Skor (Scorecard) Untuk Penilaian Sistem Grading Dan Kontes Kambing Kaligesing Imam Sanusi; Bagus Priyo Purwanto; Suryahadi Suryahadi
Jurnal Pertanian Terpadu Vol 4 No 1 (2016): Jurnal Pertanian Terpadu Jilid IV nomor 1 Juni 2016
Publisher : Sekolah Tinggi Pertanian Kutai Timur

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Abstract

ABSTRAK Tujuan penelitian ini adalahmembuat kartu skor (score card) untuk penilaian sistem grading dan kontes kambing kaligesing. Hasil penelitian menunjukkan bahwa terdapat tiga faktor determinan dalam sistem grading kambing kaligesing yang telah lama berlaku di tingkat peternak, yaitu faktor ukuran tubuh, profil muka dan leher, serta ukuran telinga. Penilaian eksterior pada kambing perah biasanya menggunakan kartu skor yang seragam dan telah dibakukan. Kartu skor dibuat berdasarkan ketiga faktor determinan sistem grading menurut peternak. Kartu skor terdiri atas komponen-komponen penilaian kuantitatif dan kualitatif. Komponen kuantitatif meliputi ukuran badan dan ukuran telinga dengan bobot total 70%, komponen kualitatif meliputi profil kepala dan leher dengan bobot total 30%. Komponen ukuran badan memiliki bobot terbesar (55%) dibandingkan dengan komponen yang lainnya. Hal tersebut dimungkinkan karena ukuran badan banyak digunakan dalam proses jual beli Kambing PE, misalnya tinggi pundak dan bobot badan (Kambingetawa 2011). Komponen profil kepala dan leher memiliki bobot 30%. Semakin cembung mukanya maka semakin dicari para penggemar Kambing Kaligesing. Demikian pula dengan gelambir, semakin bergelambir semakin menarik. Komponen ukuran telinga memiliki bobot 15% dari total penilaian. Ukuran telinga memberikan kontribusi bobot terhadap penilaian keseluruhan dari seekor Kambing Kaligesing karena merupakan ciri khas Kambing PE dan Kambing Kaligesing. Semakin panjang dan lebar telinga kambing, maka semakin tinggi skor nilai yang diperoleh.
Strategi Suplementasi Protein Ransum Sapi Potong Berbasis Jerami dan Dedak Padi B.W H.E Prasetiyono; . Suryahadi; T Toharmat; R Syarief
Media Peternakan Vol. 30 No. 3 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

ice straw and bran are low in protein. This study examined protein supplement (SPN) composed of CASREA (cassava-urea complex) and SOYXYL (protected-protein soybean meal) in rice straw and bran based ration offered to beef cattle. Experiment 1: Casrea1 (no extruded 32% urea and 58% cassava), Casrea2 (extruded 22% urea and 68% cassava), Casrea3 (extruded 27% urea and 63% cassava), and Casrea4 (extruded 32% urea and 58% cassava) were incubated in ruminal fluid. Experiment 2: Protected-protein soybean meal with xylose from Black Liquor’s (BL) of 0, 3, 6, or 9% and extruded at 120, 150, or 180oC, were incubated in ruminal fluid. Experiment 3: The best treatments of experiments 1 and 2 were used as SPN. Sixteen dairy cattle bulls aged 12-15 months were divided into 4 blocks to receive one of the following treatments: R0= rice straw and bran, R1= R0 with SPN A, R2= R0 with SPN B, R3= R0 with SPN C. SPN A, B and C composed of CASREA:SOYXYL in ratio of 20:80, 50:50, and 80:20, respectively. Casrea 2 had the highest microbial protein and post rumen protein digestibility of 29.04 mg and 76.16%, respectively. Protected-protein soybean meal with xylose from BL 3% and extruded at 1500C had the highest microbial protein and post rumen protein digestibility. SPN increased dry matter, organic matter and protein intake and their digestibility, ration efficiency, and daily gain. The highest daily gain (0.85 kg.d-1), ration efficiency (11%), and income over feed cost (Rp 7500 head-1.d-1) were R3. R3 had lower methane energy compare to R0. The result indicated that JDP supplemented with SPN (80% CASREA and 20% SOYXYL) improved ration efficiency and performance of cattle. Key words: rice straw, rice bran, cassava, urea, soybean meal
Nisbah Sinkronisasi Suplai N-Protein dan Energi dalam Rumen Sebagai Basis Formulasi Ransum Ternak Ruminansia . Hermon; . Suryahadi; K G Wiryawan; S Hardjosoewignjo
Media Peternakan Vol. 31 No. 3 (2008): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The experiment was conducted to determine the optimum synchronization ratio of N-protein and fermented organic matter (OM) in the rumen for the purpose of ration formulation for ruminant animals, in order to improve efficiency of rumen microbial N synthesis and feed efficiency. Nylon bag technique was adopted to determine ruminal characteristic of protein and OM degradation of feedstuffs (forage and concentrate diet) for which the synchronized index of N-protein and fermented OM in the rumen might be determined. By randomized block design, twelve local cattle were arranged to four groups. Each group was fed three types of diet that was different in synchronization ratio of supplying N-protein and fermented OM in the rumen, namely 20 g N/kg OM (R20); 25 g N /kg OM (R25); and 30 g N /kg MO (R30). The diets had iso-energy and iso-protein contents and had the same synchronization index. The results showed that the type of diet had no effect (P>0.05) on intake and digestibility of nutrients, N retention, average daily gain (ADG), and feed efficiency. But R20 had better parameter values than those of R25 and R30. It might be concluded that the diet having a synchronization ratio of 20 g N/kg fermented OM in the rumen will generate more efficient protein synthesis of rumen microbes and feed efficiency. Key words: synchronization index, microbial protein synthesis, ruminal characteristic
Preferensi Pakan dan Kebutuhan Nutrien Anoa Gunung (Bubalus quarlesi Ouwens 1910) pada Kondisi Prabudidaya M Basri; . Suryahadi; T Toharmat; H S Alikodra
Media Peternakan Vol. 31 No. 1 (2008): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The aim of this study was to obtain information in relation to kind of forest plants which were usually consumed by mountain anoa in its natural habitat and community area around the forest, and to estimate energy and nutrient requirements for maintenance and growth of anoa. This study was conducted in Province of Central Sulawesi using six anoas. Digestal analysis method was used to study kind of forest plants in the rumen of anoas. Cafetaria system was used to obtain information related to the dry matter intake of forest plants. Regression analysis was used to estimate energy and nutrient requirements of anoa. The results showed that anoa consumed Ficus vasculosa Rump (fruits), Scleria purpurescens (leaves), Ficus sp. (shoots), Brachiaria mutica (leaves) and Ipomea aquatica Fordk (leaves and stems). The dry matter intake of these feeds were 29.1 ± 8.5, 16.5 ± 3.8, 14.2 ± 2.8, 7.6 ± 1.7 and 4.8 ± 0.6 g/kg W0.75, respectively. The energy and nutrient requirements of anoa for maintenance based on g/kg W0.75 were 22 g TDN, 6.3 g protein, 0.457 g Ca and 0.427 g P. The energy and nutrient requirements of anoas for growth based on g/kg weight gain were 1333.64 g TDN, 399.9 g protein, 29.38 g Ca and 26.85 g P. Key words: anoa, feed preference, nutrient requirements
Kualitas Daging Kancil (Tragulus javanicus) D Rosyidi; E Gurnadi; R Priyanto; Suryahadi Suryahadi
Media Peternakan Vol. 33 No. 2 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1517.395 KB) | DOI: 10.5398/medpet.2010.33.2.95

Abstract

Lesser mouse deer is a native animal, which is commonly found in the forest of Java and information concerning this animal is still limited. This research explored the quality of lesser mouse deer meat (Tragulus javanicus). Eight lesser mouse deer (four females and four males) were used in quality aspects of lesser mouse deer meat. The physical properties observed were pH, water holding capacity, tenderness, and cooking loss. While the chemical properties observed were proximate analysis, amino acids, fatty acids, cholesterol, and EPA-DHA of the meat. The results showed that meat samples had average of ultimate pH (6.32), water holding capacity (32.82%), tenderness (1.80 kg f/m2), and cooking loss (45.15%). The average meat chemical compositions namely content of water, protein, fat, ash and cholesterol were 76.33%, 21.42%, 0.51%, 1.20%, and 50.00 mg/100 g, respectively. Fatty acids consisted of lauric, miristic, palmitic, stearic, oleic, linoleic, and linolenic acid were in the amount of 1.04% 3.09%, 30.97%, 0.77%, 59.41%, 3.22%, and 1.12%, respectively. The total EPA and DHA was 0.13% and 0.05%. Respectively, the results showed that quality aspects of  lesser mouse deer meat had a high ultimate pH, low water holding capacity, tenderness, high cooking loss, and high water content, high protein, low fat and high cholesterol.
In vitro Fermentation Characteristics and Rumen Microbial Population of Diet Supplemented with Saccharomyces cerevisiae and Rumen Microbe Probiotics Lilis Riyanti; . Suryahadi; Dwierra Evvyernie
Media Peternakan Vol. 39 No. 1 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (687.45 KB) | DOI: 10.5398/medpet.2016.39.1.40

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The objective of this study was to select three strains of probiotic Saccharomyces cerevisiae and to evaluate the effect of S. cerevisiae and rumen bacteria isolate (MR4) supplementation and their combination on rumen fermentability and rumen microbial population. Experiment 1 was designed in a 4 x 5 factorial randomized block design with 3 replications. The first factor was S. cerevisiae strain consisted of control treatment (without S. cerevisiae supplementation), NBRC 10217, NRRL Y 567 and NRRL 12618, and the second factor was incubation time consisted of 0, 1, 2, 3, and 4 h. Ration was basal ration for feedlot with forage to concentrate ratio (F:C)= 60:40. Dosage of each treatment with S. cerevisiae was 5 x 1010 cfu/kg ration. Experiment 2 was designed in randomized block design with 4 treatments: P0= basal ration of feedlot; P1= P0 + S. cerevisiae; P2= P0 + MR4 isolate (5 x 107 cfu/kg ration); P3= P0 + S. cerevisiae and MR4 isolate. The result of experiment 1 showed that supplementation of S. cerevisiae NRRL 12618 had the highest S. cerevisiae population and increased rumen bacterial population. This strain was selected as probiotic in experiment 2. The result from experiment 2 showed that probiotic supplementation stabilized rumen pH and produced the highest NH3 concentration (P<0.05) and bacterial population (P<0.05). As compared with control, all treatments reduced protozoa population (P<0.05). Combination of S. cerevisiae and MR4 probiotics produced the highest total volatile fatty acids (VFA) and isovalerate (P<0.05). It was concluded that strain S. cerevisiae NRRL 12618 had potential as probiotic yeast. Supplementation with this strain increased fermentability, rumen isoacid and decreased A:P ratio. Those abilities could be improved with MR4 rumen isolate probiotic.
Improvement of Napier Grass Silage Nutritive Value by Using Inoculant and Crude Enzymes from Trichoderma reesei and Its Effect on in Vitro Rumen Fermentation Dea Justia Nurjana; S. Suharti; . Suryahadi
Media Peternakan Vol. 39 No. 1 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (546.831 KB) | DOI: 10.5398/medpet.2016.39.1.46

Abstract

This research was aimed to evaluate the effect of inoculant and crude enzyme of Trichoderma reesei on fermentation quality and rumen digestibility of napier grass silage. This research consisted of two stages. The first stage was fermentation quality using a completely randomized design with three treatments and six replications. The second stage was rumen fermentability using a randomized block design with three treatments and six replications. The treatments of both stages were: T0= control of napier grass silage (Hi-fer+), T1= control + T. reesei inoculant at the level of 2.13 x 107 cfu/kg, and T2= control + crude enzyme from T. reesei at the level of 11.4 unit/kg. Napier grass silage were kept at room temperature and opened 21 d after fermentation. The result of the first stage experiment showed that T2 decreased (P<0.01) dry matter, organic matter, crude fiber, and neutral detergent fiber (NDF) and also increased (P<0.05) crude protein, total digestible nutrient (TDN), pH, acetic acid, and propionic acid of napier grass silage while T1 decreased (P<0.01) NDF and increased (P<0.05) propionic acid. The result of the second stage experiment showed that there were no significant differences in rumen pH, in vitro dry matter and organic matter digestibility, total VFA, proportion of VFA, ratio of acetic to propionic acids, and also NH3. It is concluded that the addition of crude enzyme from T. reesei could improve fermentation quality of napier grass silage with decreasing NDF and increasing TDN but did not affect rumen fermentability.
Improvement on the Nutritive Quality of Napier Grass Silage through Inoculation of Lactobacillus plantarum and Formic Acid Saprilian Stya Hapsari; Suryahadi Suryahadi; Heri Ahmad Sukria
Media Peternakan Vol. 39 No. 2 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (483.541 KB) | DOI: 10.5398/medpet.2016.39.2.125

Abstract

The potential availability of forage feed is high, but in reality this potential has not been able to meet the requirement of feed both in sustainable quantity and quality. Silage made with the use of liquid fermentation additive (FA) can be a solution for those problems. The use of  different levels of FA and addition of Lactobacillus plantarum bacteria as well as formic acid were expected to improve the nutritive quality of napier grass silage. The first experiment was designed to measure the fermentative quality of napier grass silage. The treatments used were the levels of FA, L. plantarum, and formic acid supplementations. The experiment used a completely randomized design with a 3x2x2 factorial arrangement with 3 replications. The first factor was the level of liquid FA (5%, 7.5%, and 10%), and the second factor was the inoculation of L. plantarum (without and with inoculation of the L. plantarum), and the third factor was the addition of formic acid (without and with the addition of 0.15% formic acid). The second experiment was aimed to evaluate chemical and microbiological characteristics, and in vitro digestibility of selected napier grass silage. The results showed that napier grass silage from all treatments showed good qualities. There were interactions between FA, L. plantarum, and formic acid on DM content (P<0.05) and ammonia production (P<0.01). The use of FA showed an interaction (P<0.01) with the addition of L. plantarum and formic acid in Fleigh point. Ammonia production in rumen (P<0.01), total VFA (P<0.05), and in vitro digestibility (P<0.01) were significantly affected by the treatments. The optimal level of liquid FA was 7.5%. Based on the nutritive quality of silage, L. plantarum addition was as effective as control treatment to improve nutritive quality of napier grass silage through the increased  of fermentation characteristics i.e., low pH, high DM product, high fermentation product (VFA), and digestible on rumen. Formic acid reduced ammonia production during ensiling and fermentation in rumen, but it was less effective in inhibiting the fermentation process when it was combined with L. plantarum.