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EFEKTIVITAS IMUNOMODULATOR EKSTRAK DAUN MACANG (Mangifera Foetida L.) TERHADAP SEL MAKROFAG TIKUS PUTIH (Rattus norvegicus) Kholifah, Sri Nur; Fitmawati, Fitmawati
Jurnal Pendidikan Matematika dan IPA Vol 11, No 1 (2020): Januari 2020
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jpmipa.v11i1.32763

Abstract

Macang (Mangifera foetida L.) has potential as an effect herbal medicinal plant with antioxidant content, namely mangiferin. The aim of this study is to prove empirically study that macang has an immunomodulatory effect through preclinical testing. This study use white mice (Rattus norvegicus) as experimental animals and Staphylococcus aureus as test bacteria. Rats were give leaf extracts of 3 varieties of orally for 7 days using 3 prescribe doses in ppm. On the 8th day, each rats was induce by an intraperitoneal section with 0,5 ml of the bacterial suspension tested. Observation of peritoneal fluid through smear preparations includes 2 parameters, phagocytosis activity and capacity. The result showed that the immunomodulatory effects on batu varieties were effective at dose 2 (10 ppm = 0,01 mg/g), limus varieties and manis varieties were effective at dose 1 (1 ppm = 0,001 mg/g). According to this research, it was concluded that leaf extracts of three varieties macang could be used as immunomodulators.AbstrakMacang (Mangifera foetida L.) mempunyai potensi sebagai tumbuhan obat herbal dengan kandungan antioksidan yaitu mangiferin.  Tujuan penelitian ini adalah membuktikan efek imunomodulator pada tiga varietas macang (batu, limus dan manis) melalui uji praklinik.  Penelitian ini menggunakan tikus putih (Rattus norvegicus) sebagai hewan uji dan Staphylococcus aureus sebagai bakteri uji.  Tikus diberi ekstrak daun tiga varietas macang secara oral selama tujuh hari menggunakan tiga serial dosis dalam satuan ppm.  Pada hari ke-8, setiap tikus putih diinduksi bagian intraperitoneal dengan 0,5 ml suspensi bakteri uji. Pengamatan cairan peritoneal melalui preparat apusan meliputi 2 parameter yaitu aktivitas dan kapasitas fagositosis.  Hasil penelitian menunjukkan bahwa efek imunomodulator pada varietas batu efektif pada dosis 2 (10 ppm=0,01 mg/g), varietas limus dan manis efektif pada dosis 1 (10 ppm=0,001mg/g). Disimpulkan bahwa daun macang (Mangifera foetida) berpotensi digunakan sebagai imunomodulator.Kata kunci : Fagositosis makrofag, Imunomodulator, Mangifera foetida
IDENTIFIKASI TUMBUHAN ANTIDIABETES BERDASARKAN ANALISIS KUANTITATIF ASAM TANAT ', Trina; ', Fitmawati; Sofiyanti, Nery
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Diabetes mellitus is a condition where the blood glucose is higher than normal condition. This condition will damage tissues in human body, and is usually caused by free radicals or by the decrease of the antioxidant defence activity. Tannic acid (tannin) is a member of antioxidant and antidiabetic agents. Antidiabetic plants used by the people are commonly unknown about their amount of tannic acid content. This study was aimed to investigate antidiabetic plants by analyzing its tannic acid content quantitatively. These compounds were compared with commercial antidiabetic drug. The determination of total tannic acid content in the plants was conducted using Folin Ciocalteu method. The results showed that the tannic acid consisted of antidiabetic drug, jackfruit bark, gambier sap, raru bark, jengkol bark, capo leaf, and sipait leaf with concentration of 21.01, 29.98, 40.95, 51.01, 51.84, 59.70 and 60.16 mg/g, respectively. The tannic acid content in the six antidiabetic plants were higher than those of commercial antidiabetic drug, therefore all of the tested plants is potential as antidiabetic drug.
STUDI ETNOBOTANI DALAM BUDAYA KULINER MELAYU RIAU DI KABUPATEN SIAK DAN UJI FITOKIMIA Supriani, Susi; ', Fitmawati; Sofiyanti, Nery
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Ethnobotany is the study of human and plant relationships such as the use of plants in culinary. However, the study of culinary herb in Indonesia including Siak, Riau Province, is still limited. The purposes of this research were to identify the plant species used in Siak Culinary and to test their phytochemical compounds. This research was conducted from Desember 2013 to Februari 2014. The sampling method used in this study was the purposive sampling method. All of the main plants used in Siak culinary were tested for their phytochemical compounds. The results showed that a total of 49 species were used in Siak Culinary, 31 species were cooked before being consumed while 18 species were freshly consumed. The phytochemical test showed that 16 species contained alkaloid, 14 species contained flavonoid, 41 species contained saponin, and 24 species contained tanin.
KEANEKARAGAMAN DURIAN (Durio zibethinus Murr.) DI PULAU BENGKALIS BERDASARKAN KARAKTER MORFOLOGI Lestari, Sri; Fitmawati, Fitmawati; Nihayatul Wahibah, Ninik
Buletin Kebun Raya Vol 14, No 2 (2011): Buletin Kebun Raya Vol. 14 (2) Juli 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Bengkalis Island is one of the areas with high diversity of durian (Durio zibethinus Murr.). This research was aimed to analyze the diversity of  durian cultivars of Bengkalis Island. It was conducted from April up to August 2010. The morphological data were analysed using NTSYS-pc 2.02 and minitab 14.13 softwares. Genetic similarity value among 36 accesions ranged from 0,28 (between BT 14, BT 15 and SB6) to 0,66 (between BT6 and BT7). The cluster analysis showed that durian diversity from Bengkalis island was 34-58% or had similarity level 42-66% and formed 7 main groups. However, they were ungroupped depend on their growth areas. Based on PCA (Principal Components Analysis) value, durian diversity was 99,7% and formed 6 groups.  Furthermore, the result of pearson correlation analysis among 77 character showed that 10 characters were positively correlated and 4 characters were negatively correlated.
Analisis Filogenetik Mangifera odorata Sumatera Tengahdan Kerabatnya Menggunakan Gen rbcL JULIANTARI, ERWINA; , FITMAWATI; SOFIYANTI, NERY
Jurnal Riau Biologia Vol 1, No 2 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Mangifera Sumatera bagian Tengah memiliki kemampuan unik dalam beradaptasi pada wilayah dengan curah hujan yang tinggi, sehingga berpotensi sebagai sumber plasma nutfah Sumatera. Secara morfologi genus Mangifera cukup sulit dibedakan karena tingginya plastisitas morfologi. Mangifera odorata diduga sebagai spesies hibrid dari M. indica dan M. foetida, sehingga diperlukan analisis untuk memperkuat status taksonomi M. odorata. Tujuan penelitian ini untuk merekonstruksi hubungan kekerabatan M. odorata dan kerabatnya berdasarkan gen rbcL. Rekostruksi menggunakan program PAUP* Versi 4.0b10 dengan metode maximum parsimony (MP). Kladogram dengan metode MP membentuk klad monofiletik dengan dua klad utama Mangifera. Klad pertama yaitu M. indica dan klad kedua terdiri dari M. foetida dan M. odorata. Kladogram mendukung asumsi bahwa M. odorata adalah hibrid dari M. foetida dan M. indica. 
KEANEKARAGAMAN DURIAN (Durio zibethinus Murr.) DI PULAU BENGKALIS BERDASARKAN KARAKTER MORFOLOGI Lestari, Sri; Fitmawati, Fitmawati; Nihayatul Wahibah, Ninik
Buletin Kebun Raya Vol 14, No 2 (2011): Buletin Kebun Raya Vol. 14 (2) July 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Bengkalis Island is one of the areas with high diversity of durian (Durio zibethinus Murr.). This research was aimed to analyze the diversity of  durian cultivars of Bengkalis Island. It was conducted from April up to August 2010. The morphological data were analysed using NTSYS-pc 2.02 and minitab 14.13 softwares. Genetic similarity value among 36 accesions ranged from 0,28 (between BT 14, BT 15 and SB6) to 0,66 (between BT6 and BT7). The cluster analysis showed that durian diversity from Bengkalis island was 34-58% or had similarity level 42-66% and formed 7 main groups. However, they were ungroupped depend on their growth areas. Based on PCA (Principal Components Analysis) value, durian diversity was 99,7% and formed 6 groups.  Furthermore, the result of pearson correlation analysis among 77 character showed that 10 characters were positively correlated and 4 characters were negatively correlated.
VARIASI INFRASPESIES MACANG (Mangifera foetida) BERDASARKAN SEKUEN GEN rbcL Rafidah, Nurul; Fitmawati, Fitmawati; Juliantari, Erwina; Sofiyanti, Nery
Al-Kauniyah: Jurnal Biologi Vol 12, No 1 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.856 KB) | DOI: 10.15408/kauniyah.v12i1.5516

Abstract

AbstrakMacang (Mangifera foetida) adalah jenis mangga kosmopolit dengan vigoritas yang kuat. Macang banyak ditemukan di perkarangan dan kebun dan bersifat semi liar. Penurunan luas lahan seperti deforestasi dapat mengakibatkan keanekaragaman kultivar macang juga mengalami penurunan secara cepat. Penelitian ini bertujuan untuk menganalisis dan merekonstruksi hubungan kekerabatan antar kultivar macang menggunakan sekuen gen rbcL. Sampel berasal dari koleksi hasil eksplorasi mangga Sumatera bagian Selatan, yaitu Provinsi Bengkulu, Lampung dan Sumatera Selatan. Ekstraksi DNA dilakukan dengan menggunakan metode CTAB yang dimodifikasi, kemudian DNA diamplifikasi dengan menggunakan primer spesifik rbcL forward dan reverse, dan dilakukan sekuensing serta analisis filogenetik. Rekontruksi pohon filogenetik menggunakan program PAUP* versi 4.0b10 dengan metode Maximum Parsimony (MP) dan Neighbor Joining (NJ). Kladogram dengan MP diperoleh dua klad, yaitu pada klad I terdiri dari kultivar Macang lonjong dan Macang lado, sedangkan klad II terdiri dari kultivar Macang bulat. Berdasarkan metode NJ, diperoleh Macang bulat memiliki jarak genetik lebih panjang sehingga dianggap sebagai individu yang lebih primitif daripada kultivar yang lain. Dengan demikian, dari penelitian ini diperoleh informasi dan bukti dari status taksonomi kultivar macang.Abstract Mangifera foetida is a species of cosmopolitan mango with strong vigor. Some M. foetida are found in some front houses and gardens and has a character of semi-wild in its cultivation. Decreases in land area, such as deforestation, can lead to a rapid decline in their diversity of cultivars. This study aimed to analyze and reconstructs the phylogenetic relationship among M. foetida cultivars using the rbcL gene sequences. Samples were collected from the exploration within the area of South Sumatra, such as Provinces of Bengkulu, Lampung and South Sumatra. The DNA extraction was carried out using the modified CTAB method, followed by DNA amplification using rbcL-specific primers, sequencing, and phylogenetic analysis. The phylogenetic trees were reconstructed using the PAUP* version 4.0b10 by using the method of Maximum Parsimony (MP) and Neighbor Joining (NJ). Cladogram of the MP tree showed two clades that the clad I consisted of M. foetida (Macang lonjong) and M. foetida (Macang lado) cultivars, whereas clad II consisted of M. foetida (Macang bulat) cultivar. The NJ tree showed that M. foetida (Macang bulat) has a longer genetic distance so it is considered as a more primitive cultivar than others. Therefore, information and evidences from the taxonomic status of the M. foetida cultivars were obtained from this study.
POTENSI LIMBAH SAGU (Metroxylon sp.) DI KECAMATAN TEBING TINGGI BARAT KABUPATEN KEPULAUAN MERANTI SEBAGAI SUBSTRAT PENGHASIL BIOGAS Anuar, Khaidir; Zul, Delita; ', Fitmawati
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Biogas is one of alternative energy sources that fulfills future fuel needs. This studyaimed to analyse the potency of sago waste as fermentation substrate for biogasproduction. This research was done experimentally by employing sago waste materialsobtained from Sago Factory Nambus River from District West Tebing Tinggi Sub-province Meranti and fresh cow rumen liquid was obtained from slaughterhouse inPekanbaru. The biogas volume was calculated by measuring the volume of water thatwas pushed every 3 days, where the volume of water driven was proportional to thevolume of biogas produced. The bacterial cell numbers was counted using plate countmethod employing Nutrient Agar (NA). The substrate fermentation temperature and pHwere measured every 3 days. The highest biogas volume was produced by fermenter III(45760 ml) containing solid sago waste, liquid sago waste, and rumen liquid with ratio 1: 1 : 1 and the lowest was produced by fermenter II (1600 ml). The total bacterialinvolved in biogas production ranges from 1,87x10 5 – 2,87x10 8 CFU/ml samples. Thetemperature and pH of substrate fermetation was relatively constant during biogasproduction. The results showed that sago waste is potential as substrate for biogasproduction.
EFEK EKSTRAK ETANOL 50 mg Tristaniopsis obovata R.Br PADA DISTRIBUSI SEL MUKUS DI USUS TIKUS JANTAN WISTAR Fitmawati, Yusfiati,
SEMIRATA 2015 Prosiding Bidang Biologi
Publisher : SEMIRATA 2015

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Abstract

Tujuan penelitian adalah mengkhaji distribusi dan area sel mukus di lapisan sel epitel usus tikus jantan Wistar (Rattus norvegicus). Sampel dibuat preparat histologis mengunakan metoda parafin dengan irisan 6 µm. Perwarnaan Hematoksilin Eosin dan PAS pH 2.5. Hasil perhitungan jumlah sel goblet untuk kontrol adalah duodenum 14 sel/mm, jejunum dan ileum 15 sel/mm. Jumlah goblet untuk dosis 50 mg ektrak etanol Tristaniopsis obovata R.Br adalah duodenum 9 sel/mm,  jejunum 14 sel/mm and ileum 20 sel/mm.  Jumlah sel goblet pada dosis 50 mg ektrak etanol berkurang untuk duodenum dan jejunum, tetapi jumlah sel goblet ileum lebih meningkat daripada kontrol. Area sel goblet untuk kontrol adalah duodenum 41,06 %/mm, jejunum 46,74 %/mm dan ileum 20,08 %/mm. Area sel goblet untuk dosis 50 mg T obovata R.Br adalah duodenum 3,46 %/mm, jejunum 18,89 %/mm dan ileum 60,09 %/mm. Kesimpulan adalah dosis 50 mg T. obovata R.Br berpengaruh terhadap kandungan mukus di sel goblet usus tikus jantan wistar, kemungkinan daerah duodenum dan jejunum  memiliki kondisi sel-selnya  yang telah mengalami kerusakan. Kata Kunci :  Sel mukus, Tikus Wistar, Usus
Immunomodulatory Effectiveness of Aqueous Obat Pahit Extract of Lingga Malay Ethnic on White Rats (Rattus novergicus) Fitmawati, Fitmawati; Roza, Rodesia Mustika; Sofiyanti, Nery; Isnaini, Isnaini; Fitri, Febrian Lailatul; Paramita, Desi; Dewi, Awal Prichatin Kusumo
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10496

Abstract

Obat pahit has been generally known and believed by Lingga Malay society as anti-aging agent. However, the study of Obat pahit is not scientifically proven. This research was aimed to prove immunomodulatory ability of Obat pahit potion from Lingga, Riau Archipelago. This study used white rats as an animal modelling, and Staphylococcus aureus as bacteria tester. The rats had been treated with aqueous Obat pahit extract from three TMPs on dose scales of 0.09, 0.18 and 0.27 mL/200g of body weight through oral administration for 7 days. Furthermore, on the 8th days, the experiment animals were injected by the preparation of bacteria tester through intraperitoneal administration in the amount of 0.5 mL/200 gram of body weigth and subsequently incubated for 1 hour after the injection. There were 2 observed parameters on this study, i.e efectivity and capacity of phagocytosis by leukocytes. The observation of leukocytes-phagocytocis activity was carried out by making a smear preparat samples of peritoneum fluid from rats. After the observation under microscope on a magnification of 100 times. The result was obtained the Obat pahit from Kalan PMT swere more effective on dose 2, while from SP4 and Linau TMPs were much more effective on dose 1. It is therefore, using these data of the results, the advanced doses scale of this Obat pahit would not be necessary. Obat pahit potion from Malay Lingga Malay Ethnic could become raw materials of immunomodulatory herbal medicine based on traditional knowledge. It also potentially as a standardized herbal.