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Microanatomical Structure and Physical Characteristics of Thin Tail Hogget with Calpastatin (CAST-1) Genotype Differences B W Putra; C Sumantri; . Nurhidayat
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1258.584 KB) | DOI: 10.5398/medpet.2013.36.2.79

Abstract

Thin tail sheep has good adaptation in tropics condition, but they have low meat quality. Quality of thin tail hogget can be improved by selection. Calpastatin (CAST) gene is an indigenous inhibitor of calpain that involved in regulation of protein turn over and growth. The objective of this research was to determine the effect of calpastatin-genotype on microanatomical structure and physical characteristics of thin tail hodget. Nine thin tail sheep from Jonggol were used for this research.PCR-RFLP method was carried out to identify genetic variation of calpastatin gene, based on the identificationof CAST variation genotype. It was found that MM and MN genotypes forcalpastatin gene with TT as asingle Calpain genotype variation. The sheep wasclustered based on the variation of calpastatin gene, 5 sheep hadMM genotype and 4 sheep hadMN genotype. Physical and microanatomical characteristics were analyzed from their meats. Sheep with MN genotype showed tougher meat, it was characterized with a greater of muscle fiber surface area, the number of muscle per muscle bundle and muscle bundle area and harder meat tenderness than in MM genotypes. Hypertrophy and hyperplasia of MN were greater than MM. 
Aktifitas dan pola makan, minum dan memamah biak kancil (Tragulus javanicus) di kebun binatang Ragunan Jakarta dan Surabaya Nurhidayat Said; Adi Winarto; Arief Boediono; Ita Djuwita; Chairun Nisa; Tutik Wrediati; Heru Setijanto; Mohamad Fakhrudin
Hemera Zoa Vol. 77 No. 1 (1995): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Telah dilakukan penelitian pada kancil (Tmgulus javanicus) di Kebun Binatang Ragunan - Jakarta dan Kebun Binatang Wonocolo - Surabaya untuk mendapatkan jenis makanan yang disukai, dan aktifitas makan, minum dan ruminasi dari kancil yang dikandangkan.Pada penelitian ini, disediakan 10 jenis makanan dan empat jenis diantaranya yaitu pisang, kacang panjang, kangkung dan pepaya lebih disukai. Aktifitas makan dilakukan pada siang dan malam hari, dan pada saat istirahat kancil melakukan aktifitas ruminasi. Perilaku makan dan ruminasi ini berbeda dengan perilakunya di habitas asalnya. Sedangkan aktifitas minum, jarang dilakukan, hal ini mungkin disebabkan oleh tingginya kadar air di dalam makanan yang disediakan. Aktifitas makan dari kancil yang dikandangkan telah berubah menjadi diurnal dan nokturnal.
Beberapa Aspek Makro dan Mikroanatomi otak tikus (Rattus sp.) yang mengalami hipotiroid Nurhidayat Said; Ita Djuwita; Koeswinarning Sigit
Hemera Zoa Vol. 77 No. 1 (1995): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Perlakuan hipotiroidosme maternal dan fetal pada tikus sampai berurnur 10 minggu, memberikan dampak pada perkembangan somatis dan otak. Secara kuantitatif, hasil penelitian ini menunjukkan penurunan bobot tubuh dan otak, volume otak. Berdasarkan berat relatif otak terhadap bobot tubuh, pertumbuhan otak tetap menjadi prioritas dalam keadaan hipotiroidisme. Beberapa parameter mikroskopik, menunjukkan penurunan tebal korteks, kepadatan serabut syaraf subkortikal, dia metersel syaraf korteks dan hipokampus juga jumlah sel syaraf dan penunjang pada korteks serebri.
PCS-16 Determaining Surgical Method by Meniscectomy Induction on Garut sheep (Ovis aries) for early stage of Osteoarthritis Handina Rakhmawati; Adrian Situmeang; . Nurhidayat; Andri Maruli Tua Lubis; Harry Murti; Arief Boediono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Osteoarthritis (OA) is the most common joint disease that cause of pain and disability by various factors such as advanced age, obesity, trauma, and arthritis disease. These factors affect by degeneration of the cartilage surface, leading to loss of matrix include proteoglycan osteophyte formation, subcondral and synovial membrane affected. In the healthy joint, meniscus, articular cartilage, subchondral bone, and synovial membrane provide support to the joint. The meniscus is an important load bearing structure and has nutritive as well as lubricating properties in the knee joint as well (Little et al. 2010).Animal models are research materials that can be used in studying potential pathogenesis and therapy in various diseases in humans. Sheep are commonly large animal model of OA because of the availability, ease of handling, and have a similarities with humans in size and structure of joint. In the development of science, sheep can be used as an animal model in studying the pathogenesis of diseases in human orthopedics studies such as joints, ligaments, and bones. Garut sheep is an Indonesian germplasm indigenous that has the structure, density, and size of joint anatomy that are similar in human joints rather than other small animals. This is the basis of the utilization of Garut sheep as an animal model in human orthopaedic. (Little et al. 2010; Gregory et al. 2012).The aim of this study was to identify and analyze the determining surgical method by meniscectomy induction on Garut sheep with 8 weeks post meniscectomy observation for early stage of OA.
PF-30 Anatomical Characteristic of Hindlimb Skeleton of Sumatran Rhino (Dicerorhinus sumatrensis) . Nurhidayat; Eni Puji Lestari; Danang Dwi Cahyadi; Chairun Nisa'; . Supratikno; Savitri Novelina; Heru Setijanto
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Sumatran rhino (Dicerorhinus sumatrensis) is an endangered animal in Indonesia. Currently, Sumatran rhino only found on Sumatera and Kalimantan in very small populations. These herbivorous animals are classified into the order of Perissodactyla (odd-toed animals) and family Rhinocerotidae [1]. Sumatran rhino is one of the largest living land mammals, reaching 1000 kg [1], although this species has the smallest body weight among all extant rhinos. This animal has a round and long body shape, relatively short legs with three digits on each leg. These body structures correspond to their habitat in the highlands, so the Sumatran rhino has an excellent ability to pass steep terrain [2]. For this reason, a strong hind limb structure is needed to push the body when walking, running and climbing the steep slopes. Therefore, this study was carried out in order to analyze the correlation between the Sumatran rhino’s hind limb skeleton and its functional roles.
KELAINAN BANGUN ANATOMIS KUKU KUDA KOLEKSI LABORATORIUM ANATOMI FKH IPB Kemaz A Dewangga; Koeswinarning Sigit; Nurhidayat -
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study is to observe the anatomical structure of horse hooves collected from the Laboratory of Anatomy FKH IPB. Twenty five hoof specimens, consisting of ten fore hooves and fifteen hind hooves were used as research materials. The external morphology such as color, angle, structure and condition of the hoof wall were described. The observation on external morphology showed that the hooves have two basic colors, black and white. Generally, all of the hoof specimens showed abnormalities in such aspect as angle, structure and condition of the wall. The structures of fore hoof and hind hoof from this study are classified into 8 categories, they are: flat foot, flared foot, knol hoef, fever rings, sand crack, club foot, contracted foot and bull nosed foot.
Kualitas Tulang Tikus Betina Normal yang Diberi Ekstrak Sipatah-patah pada Masa Pertumbuhan Sabri Mustafa; Nurhidayat -; Koeswinarning Sigit; Bambang Pontjo Priosoeryanto; Wasmen Manalu
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The experiment was designed to study the effects of sipatah-patah extract (ESP) on bone growth ingrowing female rats in order to prevent osteoporosis during postmenopausal period. Twenty growing femaleSprague Dawley rats in similar body weight were used with the average of age was 20 days old. Theexperimental rats were randomly divided into 5 (five) groups: control group (NOV-0, administered carboxylmethyl cellulose 1%) and groups administered ESP at the age of 30 days (NOV-1), 60 days (NOV-2), 90days (NOV-3), and 120 days (NOV-4) with dosage of 750 mg/kg body weight daily. Blood samples werecollected every 30 days to analyze serum calcium and phosphate concentrations. At the end of the treatment(180 day old), all of the animals were sacrificed to measure and analyze bone growth histologically. Ostibia-fibula slice was stained by using Hematoxylin Eosin (HE) method to observe osteoblast and osteoclastdensities, while Masson trichrome was used to observe trabeculae structure. The results showedadministration of ESP in rat during growing period improved bone growth. NOV-1 with longer duration(150 days) showed better growth rate with longer femur, optimum serum calcium, and phosphateconcentrations, and higher number of osteoblast with lower osteoclast densities compared to control rats(NOV-0). It was concluded that giving ESP at earlier age with longer duration during growth period couldimprove bone growth. This condition was expected could also improve the bone conditions during growingperiod.
PERTUMBUHAN DAN AKTIVITAS ANAK TIKUS PADA PEMAPARAN CAHAYA YANG BERBEDA Ida Bagus Made Suaskara; Nastiti Kusumorini; Nurhidayat -
Jurnal Biologi Udayana Vol XI, No 1
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Empat puluh lima (45) ekor anak tikus jantan umur 1 hari digunakan pada penelitian ini. Anak tikus dibagi menjadi satu kelompok kontrol dan dua kelompok perlakuan yaitu; 1) Kelompok perlakuan gelap (D) dan kelompok ini dibagi lagi menjadi 4 sub kelompok perlakuan berdasarkan jumlah hari gelap yaitu; a) 6 hari (D6), b) 12 hari (D12), c) 18 hari (D18) dan d) 24 hari (D24), dan 2) kelompok perlakuan cahaya (L) juga dibagi menjadi 4 sub kelompok yaitu; a) 6 hari (L6), b) 12 hari (L12), c) 18 hari (L18) dan d) 24 hari (L24). Hasil penelitian ini menunjukkan bahwa lama pemberian cahaya memberikan pengaruh yang nyata terhadap pertumbuhan dan perkembangan Nukleus Suprachiasmatik (NSC). Pertumbuhan anak tikus pada pemaparan cahaya yang berbeda selama periode menyusui menyebabkan rataan pertambahan berat badan baik pada kelompok perlakuan gelap maupun cahaya sangat lambat dengan meningkatnya lama perlakuan. Aktivitas anak tikus berupa (jarak tempuh, awal pergerakan, gerakan di tempat maupun total pergerakan di tempat, dan lama waktu istirahat semakin pendek), cenderung meningkat sebanding dengan lama cahaya. Sedangkan pada perlakuan gelap terjadi sebaliknya yaitu cenderung menurun. Tidak ada perbedaan pengaruh pemaparan cahaya dan gelap terhadap perubahan kadar hormon T3 dalam plasma darah tikus.