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All Journal Biology, Medicine, & Natural Product Chemistry Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI Biodidaktika : Jurnal Biologi dan Pembelajarannya Jurnal Pengabdian Pada Masyarakat Gorontalo Agriculture Technology Journal Jurnal Ilmiah Farmasi Farmasyifa Jurnal Lingkungan dan Sumberdaya Alam (JURNALIS) International Journal Mathla'ul Anwar of Halal Issues Biological Environment and Pollution Jurnal Pengabdian Pada Masyarakat Jurnal Ilmiah Kesehatan Delima Jurnal Ilmu Pertanian dan Perkebunan

Hadi Susilo

Universitas Mathla'ul Anwar

Author-ID : 2115519

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The Diversity and Abundance of Egg Parasitoids in Brown Planthopper, Nilaparvata lugens Stål. (Hemiptera: Delphacidae) at Different Rice Growth Phases in Saketi, Pandeglang, Banten Nurullah Asep Abdilah; Hadi Susilo
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.39050

An extensive study about egg parasitoid biodiversity in different plant growth stages is an important need. This study was aimed to determine the diversity and abundance of egg parasitoids Nilaparvata lugens on different rice growth stages in Saketi, Pandeglang, Banten. The parasitoids sample was collected three times with two days interval sampling in three villages, i.e. Sodong (location I), Sindanghayu (location II), and Sukalangu (location III) from February to September 2018. Ten plants (as bait: rice that infected by N. lugens eggs) were put during two days along the transect line (100 meters) with 10 m space in between. The findings revealed that the abundance richness (S), the diversity index (H'), and similarity (J') of egg parasitoids of N. lugens were higher in the location I than those in the locations II and III. The study indicated that the diversity and abundance of egg parasitoids of N. lugens decreased in the generative phase of rice growth.

ISOLASI GEN SITRAT SINTASE BAKTERI Pseudomonas aerugenosa PS2 DARI RIZOSFER POHON KRUING (Dipterocarpus sp.) UNTUK MODEL KONSTRUKSI METABOLISME SEL MIKROALGA BERKARBOHIDRAT RENDAH Dwi Susilaningsih; Asahedi Umoro; Fredrick Onyango Ochieng; Dian Noverita Widyaningrum; Hani Susanti; Hadi Susilo; I Nengah Swastika; Utut Widyastuti
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.2967

Pseudomonas has the potential ability for production of citrate synthase synthesis. Pseudomonas aeruginosa could synthesize the enzyme of citrate synthase which is most likely compatible with microalgae cell. Pseudomonas aerugenosa can be found in the rhizosphere of Kruing (Dipterocarpus sp., Dipterocarpaceae). This bacteria is commonly used in agriculture purposes because it is able to synthesize organic acid such as citric acid. These organic acids are synthesized from a reaction between oxaloacetate and acetyl CoA, catalyzed by citrate synthase (CS) in the tricarboxylic acid cycle (TCA). Rhizosphere as microbial sources was obtained from Kruing (Dipterocarpus sp.), which was collected from ‘Carita’ Research Forest, Pandeglang, Banten, West Java. Citrate synthase gene-specific primers were designed based on citrate synthase gene sequences as depicted in Genbank. The isolation and amplification showed that citrate synthase can be detected and purified from Pseudomonas aeruginosa target and it consists of 1600 bp and encodes 509 amino acids. Based on BLAST (Basic Local Alignment Search Tool) analysis, CS genes that were successfully isolated had 92 % similarity with Pseudomonas aeruginosa type II citrate synthase. This CS gene is expected to be expressed in microalgae metabolism to divert the metabolism of carbohydrate formation into fatty acids.

ANALISIS POTENSI BUDIDAYA TANAMAN TRANSGENIK DI INDONESIA Hadi Susilo
Jurnal Lingkungan dan Sumberdaya Alam (JURNALIS) Vol 2 No 1 (2019): environmental
Publisher : Program Studi Teknik Lingkungan, Fakultas Teknik, Universitas Banten Jaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.728 KB)

Tanaman transgenik adalah tanaman hasil rekayasa gen yang dibuat dengan cara disisipi satu atau sejumlah gen dari organisme lain, dengan tujuan untuk diperoleh sifat baru yang unggul dan diinginkan, misalnya resisten terhadap cekaman kekeringan, resisten terhadap hama, resisten terhadap herbisida. Produk teknologi DNA dikenal dengan Produk Rekayasa genetika (PRG), sebagai contoh adalah kedelai transgenik Tujuan penelitian ini untuk menganalisis potensi budidaya tanaman transgenik di Indonesia. Metode yang digunakan dalam studi ini adalah tinjauan pustaka (review) dengan menggali data dan informasi yang dikumpulkan dari berbagai sumber meliputi: undang-undang, peraturan pemerintah, surat keputusan, artikel dalam jurnal, laporan hasil penelitian. Hasil penelitian ini adalah aplikasi tanaman transgenik dilapangan perlu manajemen yang dapat mengatur pola tanam tanaman transgenik dan non transgenik, penyediaan bibit tanaman transgenik dikendalikan dalam bentuk paket kemasan, dan perlunya kepastian hukum dan dukungan pemerintah terhadap produk transgenik.

BIODIVERSITAS LABA-LABA ARACHNIDA (Araneae) DI KAWASAN EKOSISTEM DESA WISATA BANYUBIRU KECAMATAN LABUAN KABUPATEN PANDEGLANG Hadi Susilo; M Nasrun Hakim; Usman Setiawan
Jurnal Lingkungan dan Sumberdaya Alam (JURNALIS) Vol 4 No 1 (2021): JURNALIS
Publisher : Program Studi Teknik Lingkungan, Fakultas Teknik, Universitas Banten Jaya

Biodeversitas dan klasifikasi jenis laba-laba dapat menunjang ekowisata di Kabupaten Pandeglang, Provinsi Banten. Tujuan dari penelitian adalah untuk mengetahui jumlah jenis laba-laba (Araneae) di kawasan desa wisata Banyubiru. Pengumpulan sampel laba-laba dilakukan dengan metode ekplorasi. Analisis data keanekaragaman diuraikan secara deskriptif. Hasl penelitian menunjukkan bahwa keanekaragaman laba-laba Arachnida (Araneae) pada Desa Wisata Banyubiru Kecamatan Labuan Kabupaten Pandeglang diperoleh sebanyak 70 individu yang termasuk dalam 5 famili dan 10 spesies yaitu: A. argentata (Araneidae), Gasteracantha sp (Araneidae), A. apensa (Araneidae), A. trifasciata (Araneidae), W. saltabundus (Theridiidae), N. inaurata (Nephilidae), N. maculata (Nephilidae), L. reclusa (Sicaridae), P. paykulli (Salticidae) dan Hasarius sp (Salticidae). Keanekaragaman laba-laba Arachnida (Araneae) dari tiga stasiun di Desa Wisata Banyubiru Kecamatan Labuan Kabupaten Pandeglang menunjukkan indeks keragaman (H) berkategori sedang, indeks kemerataan tinggi dan indeks dominansi yang kecil. Ekosistem desa wisata masih lestari

KEANEKARAGAMAN MIKROALGA SEBAGAI BIOINDIKATOR PENCEMARAN DI SITU CIBANTEN KECAMATAN CIOMAS KABUPATEN SERANG BANTEN Rt Inu Rahayu; Hadi Susilo
Jurnal Lingkungan dan Sumberdaya Alam (JURNALIS) Vol 4 No 2 (2021): Jurnal Lingkungan dan Sumberdaya Alam (JURNALIS)
Publisher : Program Studi Teknik Lingkungan, Fakultas Teknik, Universitas Banten Jaya

Mikroalgae dapat berperan sebagai bioindikator pencemaran air dan mempunyai banyak manfaat antara lain: sebagai komponen pangan, bahan obat, dan kosmetik. Tujuan penelitian ini untuk mengetahui kelimpahan spesies miroalgae di Situ Cibanten Ciomas Kabupaten Serang. Penelitian menggunakan metode purpossive sampling, sampel diambil pada pagi hari jam 08.00-12.00. Sampel disaring menggunakan plankton net dengan ukuran mesh 50 µm. Sampel kemudian ditambahkan dengan formalin 4 % dan diamati dengan menggunakan mikroskop di Laboratorium Terpadu Fakultas Sains, Farmasi, dan Kesehatan Universitas Mathla’ul Anwar Banten. Indeks diversitas tertinggi didapatkan di lokasi satasiun 1 dengan nilai 3.11. dan terendah pada stasiun 2 sebesar 2.360. Indek Kemerataan tertinggi pada stasiun 1 sebesar 0.978 dan terendah pada stasiun 2 sebesar 0.803. Indeks dominasi dari masing-masing spesies mendekati nilai 0, yang berarti nilai dominansinya rendah. Indeks kelimpahan relatif ditemukan pada spesies Spirogyrasp is of 17.7%, dan yang terendah adalah spesies Phacuslongicaida and Lemaneaannulata 0.4%. Situ Cibanten mengalami pencemaran sedang

IDENTIFIKASI TELUR CACING PARASIT PADA FESES HEWAN TERNAK DI PROPINSI BANTEN Hadi Susilo; Nurullah Asep Abdillah; Kiki Rizki Amelia
Biodidaktika : Jurnal Biologi dan Pembelajarannya Vol 15, No 2 (2020)
Publisher : Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30870/biodidaktika.v15i2.8719

Hewan ternak mamalia seperti: sapi, kambing, kerbau dan kelompok unggas seperti: ayam dan bebek memiliki peran penting, salah satunya untuk kebutuhan pangan. Tujuan penelitian ini adalah untuk mengetahuidan mengidentifikasi telur cacing parasit pada feses hewan ternak Di Propinsi Banten. Metode yang digunakan dalam penelitian ini adalah metode flotasi dan sedimentasi dengan menggunakan sampel feses hewan ternak (sapi, domba dan kerbau). Hasil penelitian menunjukan bahwa hewan ternak di Provinsi Banten terinfeksi Nematoda, Trematoda dan Cestoda pada fesesnya. Telur cacing parasit yang ditemukan sebanyak 9 jenis, yaitu: Haemochus, Trichostronylus, Toxocara, Cooperia,Trichiuris trichiura, Strongyloid sp.,Moniezia sp.,Fasciola sp.dan Paramphistomum sp. Prevalensi tertinggi dan intensitas tertinggi dari jenis Haemochus. Infeksi pada ternak juga dapat terjadi secara tunggal atau campuran (terdiri atas dua atau lebih cacing parasit). Prevalensi tertinggi adalah infeksi tunggal Nematoda 56,25% dan infeksi campuran (kombinasi Trematoda dan Nematoda) sebanyak 28,75%. Tingkat prevalensi dan intensitas telur cacing parasit di hewan ternak di Provinsi Banten tergolong rendah.

MOUTHWASH OF Amaranthus hybridus L. LEAF EXTRACT WITH ETHYL ACETATE AS A Streptococcus mutans ANTIBACTERIAL Yelfi Yelfi; Hadi Susilo; Nia Marlina Kurnia
Jurnal Ilmiah Farmasi Farmasyifa Vol 5, No 1 (2022)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/jiff.v5i1.8379

Caries and dental plaque are oral and dental problems. Green spinach (Amaranthus hybridus L.) can be used as medicine and has antibacterial activity. It also benefits human health and is a nutrition source. The phytochemical results of the leaf extract showed that A. hybridus L. contains alkaloids, flavonoids, steroids, saponins, and tannins. This study aimed to determine the inhibitory action of A. hybridus L. leaf extract against the growth of Streptococcus mutans and to formulate A. hybridus L. leaf extract in a mouthwash. The disk diffusion method and brain heart infusion agar medium were used in this study, with five treatment groups: extract concentrations of 25%, 50%, and 100%, positive and negative control were studied. Physical tests, including organoleptic, pH, and viscosity tests, were performed. The pH of A. hybridus L. leaf extract in the mouthwash was 4.80, with a viscosity of 1.2456–1.378 cP. The inhibition zones of antibacterial activity against S. mutans in the extract concentrations of 25%, 50%, and 100% were 1.13, 1.73, and 2.90 mm, respectively. The ethyl acetate extract of A. hybridus L. leaves in mouthwash displayed antibacterial activity against S. mutans, with an average inhibition zone of 0.57 mm. The mouthwash formulation produced an inhibition zone with an average F1 of 0.57 mm and F2 of 1.67 mm.

REAL TIME POLYMERASE CHAIN REACTION (RT-PCR) FOR DNA PIG CONTAMINATION IDENTIFICATION SAUSAGE IN SIX DISTRICT PANDEGLANG BANTEN: IDENTIFIKASI KONTAMINASI BABI PADA SOSIS DENGAN MENGGUNAKAN REAL TIME POLYMERSE CHAIN REACTION (RT-PCR) DI 6 (ENAM) KECAMATAN KABUPATEN PANDEGLANG BANTEN Hadi Susilo
International Journal Mathla’ul Anwar of Halal Issues Vol. 1 No. 1 (2021)
Publisher : Universitas Mathla’ul Anwar Banten

Sausage is a meat product processed that is popular food especially in Pandeglang, Banten Province. The importance of halal certificates or the existence of the MUI (Indonesian Ulama Council) halal logo for processed meat products makes Muslim people confident to consume them. The aim this research was to identify pig DNA contamination in sausage products in six districts in Pandeglang without the MUI halal labels using RT-PCR (Real Time-Polymerase Chain Reaction). RT PCR that can calculate to pig to fill these sample free from pig contamination. This research was divided into two stage, the first stage is extracted or carried out DNA and the second stage is RT PCR analysis. The results of the DNA purity test on sausage samples had DNA purity values of 1.84-1.9 (A260 / A280) and resulted in sample concentrations ranging from 37.8 to 102.5 ng / µl. The only amplification on the FAM curve was in the positive control pig. the Cq value ranges from 30 - 31.29. The results of RT PCR on sausage samples in the district area in Pandeglang Banten did not detect the presence of pig DNA.

HALAL AND THAYYIB FOOD IN ISLAMIC SHARIA PERSPECTIVE Ali Farkhan Tsani; Hadi Susilo; Suyamto Suyamto; Usman Setiawan; Sudanto Sudanto
International Journal Mathla’ul Anwar of Halal Issues Vol. 1 No. 1 (2021)
Publisher : Universitas Mathla’ul Anwar Banten

Consumption of halal and thayyib food will make it easier for someone to do good deeds, this is mentioned in the Qur'an Surah Al Maidah verse 88. Halal is goods that are not haram, consuming them is not prohibited by religion. At least, the prohibition can be divided into two aspects. First, haram in essence or materially has been declared haram by the Shari'a, such as pigs, carrion, and blood. Second, haram is not in essence, but can be from the way of buying, obtaining, or processing the goods. Thayyib means something that is holy, not unclean, and not disgusting which is not liked by the soul. Whoever eats unlawful food, whether he wants it or not, his limbs will tend to sin, whether he realizes it or not. Whoever eats halal food, surely his limbs will obey and be given God’s guidance to do good.

Pemanfaatan Limbah Serbuk Gergaji Sebagai Media Budidaya Jamur Tiram (Pleourotus Ostreatus L.) Hadi Susilo; Riki Rikardo; Suyamto Suyamto
Jurnal Pengabdian Pada Masyarakat Vol 2 No 1 (2017)
Publisher : Universitas Mathla'ul Anwar Banten

The development of the agricultural sector is not only food crops, but also plantation crops and horticulture. One of horticulture crops that have contributed to human is oyster mushroom. Oyster mushroom is highly suitable for people of Indonesia that is still difficult to meet the needs complete nutrition, especially protein needs. Contains essential amino acids: arginine, histidine, isoleucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, unsaturated fats, vitamin B1, B2, niacin, biotin, micro and macro elements. The oyster mushroom is also shown to be potent inhibitors of HIV-AIDS, cholesterol, blood sugar and cancer. Mushroom cultivation media requires for growth. In this study utilizes waste sawdust which there are many in the community with the goal of increasing the economic value and the standard of life. Cultivation starts from media preparation, sterilization, filling and incubation seed to harvest. Wet harvest oyster mushrooms for 5 weeks increased gradually. 1000 new baglogs, 750 baglogs already can produce 290 kilograms of harvested oyster mushrooms wet. The selling price is Rp 9,000 and total revenue is Rp 2.61 million for one month. Mushroom cultivation should be accompanied by the processing of waste in order to have a double added value of improving economic value and overcome the environment. Based on the data and the results of the discussion showed that the oyster mushroom cultivation can work well and provide benefits, so as to improve the economy for the community.

Co-Authors A Suhaemi Abdilah, Nurullah Asep Agus Setiawan Ai Widiyawati Ali Farkhan Tsani Arini Khaerunnisa Asahedi Umoro Candra Junaedi Cory Novi dan Nursuciyoni, Tuti Rostianti Maulani, Dini Nur Hakiki Dhyneu Dwi Jayantie Dian Noverita Widyaningrum Dimas Danang Indriatmoko Dwi Marwati Juli Siswanti Dwi Susilaningsih Eka Yulli Kartika Eneng Elda Ernawati Firman Rezaldi Fredrick Onyango Ochieng Gina Siti Nurhayati Hani Susanti Herlina Siti Nurantika I Nengah Swastika irfan mujahidin Kiki Rizki Amelia Lia Amelia Pertiwi M Nasrun Hakim Marlinda Indriati Mu'jijah Mu'jijah Mu’jijah Mu’jijah Nia Marlina Kurnia Nurullah Asep Abdillah Riki Rikardo Riki Rikardo Roudotussaadah Roudotussaadah Rt Inu Rahayu sudanto sudanto Suyamto Suyamto Suyamto Suyamto Suyamto Suyamto Swastika Oktavia Tarso Rudiana, Tarso Usman Setiawan UTUT WIDYASTUTI Yelfi Yelfi Yuningsih Yuningsih Yusransyah Yusransyah

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