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All Journal Vegetalika Jurnal Ilmu Pertanian EUGENIA Agrotek Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian dan Pengembangan Pertanian Buletin Tanaman Tembakau, Serat & Minyak Industri AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Informatika Pertanian Jurnal Penelitian Pertanian Tanaman Pangan jurnal Ilmu Pertanian (Agricultural Science) Buletin Tanaman Tembakau, Serat & Minyak Industri Jurnal Penelitian dan Pengembangan Pertanian Agroista: Jurnal Agroteknologi
Panjisakti Basunanda
Faculty Of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Immunology & microbiology Materials Science & Nanotechnology

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ESTIMATES OF GENETIC VARIANCE COMPONENT OF AN EQUILIBRIUM POPULATION OF CORN Hadini, Hamirul; Nasrullah, Nasrullah; Basunanda, Panjisakti; Taryono, Taryono
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

There are abundant maize populations in Hardy-Weinberg equilibrium, which can be used as source of gene to develop either a hybrid variety or an open pollinated variety. Genetic parameters of a population, such as additive genetic variance and variance due to dominance which can be estimated using North Carolina Design I, were used to decide a breeding method to be applied. The objectives of this research were to estimate the genetic variance component of important quantitative traits in an equilibrium corn population and to decide the best method to develop the population. The research was conducted at Agricultural Station of Agriculture Faculty, Gadjah Mada University, in 2012 which was carried out in two steps: to develop  half-sib and full-sib families and to evaluate both families in randomized complete block design with three replications. The data was subjected to analysis of variance, and was followed by estimation of genetic variances. The additive variance of ear length and ear diameter of the corn population was greater than dominance variance. In contrary to kernel weight/ear, the dominance variance was greater than additive variance. So based on kernel weight/ear, the Hardy-Weinberg equilibrium corn population can be developed through inbreeding to form a hybrid variety.Keywords: breeding, corn, genetic variance
Hubungan Antara Hasil dan Komponen Hasil Wijen (Sesamum indicum L.) pada Generasi F1 dan F2 Persilangan Sbr2, Sbr3, dan Dt36 Adikadarsih, Sri; Permata, Siska; Taryono, .; Suyadi, .; Basunanda, Panjisakti
Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 7, No 1 (2015): April 2015
Publisher : Balai Penelitian Tanaman Pemanis dan Serat (Balittas)

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Abstract

Dalam program pemuliaan tanaman wijen, informasi keragaman genetik dan hubungan antarsifat sangat penting untuk menentukan keberhasilan seleksi. Penelitian yang bertujuan untuk mempelajari keragaman genetik dan hubungan antara komponen hasil dan hasil wijen pada generasi F1 dan F2 persilangan Sbr 2, Sbr 3, dan Dt 36 telah dilaksanakan dari bulan November 2012 sampai dengan Februari 2013 di Padangan, Sitimulyo, Piyungan, Bantul, Yogyakarta. Penelitian menggunakan rancangan acak lengkap (RAL) dengan tiga ulangan. Bahan tanam yang digunakan adalah benih tetua, F1, dan F2 hasil persilangan antara Sbr 3 x Sbr 2, Sbr 3 x Dt 36, Sbr 2 x Dt 36, dan resiproknya. Benih bulk hasil persilangan ditanam secara rapat dalam baris pada petak-petak yang berukuran 4 x 1 m. Pengamatan dilakukan pada parameter tinggi tanaman, jumlah cabang, jumlah polong, berat polong, berat biji, jumlah ruas, panjang ruas, umur berbunga, umur panen, dan berat 1.000 biji. Hasil penelitian menunjukkan bahwa komponen hasil yang memiliki keragaman genetik besar adalah berat biji per tanaman (68,437%), berat polong (40,532%), jumlah cabang (33,251%), jumlah polong (30,269%), dan tinggi tanaman (21,256%). Nilai heritabilitas yang tinggi terdapat pada tinggi tanaman (65,52%) dan umur panen (55%). Komponen hasil yang memiliki korelasi nyata terhadap hasiladalah jumlah cabang, jumlah polong, berat polong, dan umur berbunga, sedangkan yang berpengaruh langsung terhadap hasil wijen adalah jumlah cabang dan berat polong. In sesame breeding program, information about genetic variations and relationships ammongs characters is very important to determine the success of line selection. Studies about correlation between yield and yieldcomponents of F1 and F2 from crosses of Sbr 2, Sbr 3, and Dt 36 was conducted on November 2012 to February 2013 in Padangan, Sitimulyo, Piyungan, Bantul, Yogyakarta. The treatments were arranged in completerandom design (CRD) with three replications. Planting materials used were the seed of parents, F1, and F2 from crossing between Sbr 3 x Sbr 2, Sbr 3 x Dt 36, Sbr 2 x Dt 36, and their reciprocals. Bulk breeding seeds planted in rows in high density to reach maximum populations as the genetic resource in the plots according to its genotypes. The observation was made on plant height, number of branches, number of pods, weight of pods, number of nodes, nodes length, day of flowering, plant maturing age, and 1,000seed weight. The results showed that, components which showed high genetic variation were weight of seeds per plant (68.437%), weight of pods (40.532%), the number of branches (33.251%), number of pods(30.269%), and plant height (21.256%). High heritability values was shown in parameters of plant height (65.52%) and plant maturing age (55%). Yield components which have significant correlation with the yield were number of branches, number of pods, pod weight, and days to flowering, while those have a direct effect on the yield of sesame are the number of branches and pods weight.
PREDIKSI GENOTIPE TETUA JAGUNG BERBULIR UNGU BERDASARKAN KESESUAIAN NISBAH HARAPAN PADA BULIR S1 DAN S2 Pamandungan, Yefta; Purwantoro, Aziz; Basunanda, Panjisakti
EUGENIA Vol 18, No 3 (2012)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.18.3.2012.4097

Abstract

ABSTRACT   The research was aimed to determine the parent genotypes of purple corn by the similarity of expected ratio on kernels S1 and S2 using four locus models, Pr/pr, C/c, R/r and Y/y genes. The study was conducted in two phases, namely, 1) Making the individuals of selfing to-1 (S1), and 2) Making the individuals of selfing to-2 (S2). Observed data in the form of kernel per ear of corn was separated by the characters of purple, yellow and white color then analyzed by using the Chi-square Test. The results showed that the offspring genotype from selfing on the base population can be predicted by looking at the suitability between the offspring and parental genotypes based on the ratio of expectation. Parent genotypes prediction on the base population of selfing were PrPrCcRrYy, PrPrCcRryy, PrPrCcRrYY, PrPrCcRRYY and PrPrCCRrYY. Keywords: parent genotypes, purple kernel, corn ABSTRAK Tujuan penelitian ini adalah mengetahui genotipe tetua jagung berbulir ungu berdasarkan kesesuaian dengan nisbah harapan pada bulir S1 dan S2 menggunakan model empat lokus yaitu gen Pr/pr, C/c, R/r dan Y/y. Penelitian dilakukan dalam dua tahap yaitu, 1) pembuatan individu hasil selfing ke-1 (S1), dan 2) pembuatan individu hasil selfing ke-2 (S2). Data hasil pengamatan berupa bulir jagung per tongkol yang dipisahkan berdasarkan karakter warna ungu, kuning dan putih selanjutnya dianalisis dengan menggunakan uji khi kuadrat. Hasil penelitian menunjukkan bahwa genotipe keturunan hasil selfing pada populasi dasar dapat diprediksi dengan melihat kesesuaian antara genotipe keturunan dan tetua berdasarkan nisbah harapan. Prediksi genotipe tetua pada populasi dasar selfing adalah PrPrCcRrYy, PrPrCcRryy, PrPrCcRrYY, PrPrCcRRYY dan PrPrCCRrYY. Kata kunci: genotipe tetua, berbulir ungu, jagung
BERAS HITAM SUMBER ANTOSIANIN DAN PROSPEKNYA SEBAGAI PANGAN FUNGSIONAL Kristamtini, Kristamtini; Taryono, Taryono; Basunanda, Panjisakti; Murti, Rudi Hari
Jurnal Penelitian dan Pengembangan Pertanian Vol 33, No 1 (2014): Maret 2014
Publisher : Badan Penelitian dan Pengembangan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jp3.v33n1.2014.p17-24

Abstract

Beras hitam merupakan sumber antosianin yang tinggi dan murah dibandingkan dengan sumber antosianin lain seperti bluberi dan anggur. Antosianin merupakan senyawa organik golongan flavonoid dengan struktur utama tiga gugus aromatik. Sebagai antioksidan, antosianin bermanfaat bagi kesehatan, di antaranya untuk mencegah penuaan dini, melindungi lambung dari kerusakan, menghambat sel tumor, sebagai senyawa antiinflamasi dan antikanker, melindungi otak dari kerusakan, mencegah obesitas dan diabetes, meningkatkan kemampuan memori otak, mencegah penyakit neurologis, dan menangkal radikal bebas dalam tubuh. Beras hitam juga mengan-dung protein, vitamin, dan mineral yang lebih tinggi daripada beras putih. Berdasarkan hal tersebut, diperlukan penjelasan tentang struktur kimia antosianin, sifat antosianin dan manfaatnya bagi kesehatan dan aplikasi industri, beras hitam sebagai sumber antosianin, distribusi dan sintesis antosianin, manfaat antosianin bagi tanaman, dan prospek pengembangan beras hitam di Indonesia.
STRUKTUR GENETIKA POPULASI JAGUNG DALAM KESEIMBANGAN HARDY-WEINBERG DAN SILANG KERABATNYA Hadini, Hamirul; Nasrullah, Nasrullah; Taryono, Taryono; Basunanda, Panjisakti
Agro-UPY Vol 6, No 2 (2015): Agro-UPY
Publisher : Prodi Agroteknologi, Fakultas Pertanian, Universitas PGRI Yogyakarta

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Abstract

Intisari   Struktur genetika suatu populasi jagung penting diketahui dalam pemuliaan jagung,  baik  dalam perakitan varietas jagung bersari bebas maupun varietas hibrida.  Populasi dasar yang berlimpah jumlahnya di alam dan sering digunakan untuk pengembangan jagung unggul adalah populasi dalam keseimbangan Hardy-Weinberg, suatu populasi yang struktur genetiknya stabil, yaitu tidak terjadi perubahan frekuensi alel dari generasi ke generasi.  Tulisan ini akan menjelaskan larik genotip, rerata, dan ragam suatu populasi silang acak (F=0) dan perubahan-perubahan pada keturunannya sampai pada koefisien silang (F) = 1, F, dan ½ . Kata Kunci: Jagung, Struktur genetik, Keseimbangan Hardy-Weinberg
ANALISIS STABILITAS HASIL GENOTIPE KEDELAI MENGGUNAKAN METODE ADDITIVE MAIN EFFECT AND MULTIPLICATIVE INTERACTION (AMMI) Krisnawati, Ayda; Basunanda, Panjisakti; Nasrullah, nFn; Adie, M Muchlish
Informatika Pertanian Vol 25, No 1 (2016): JUNI 2016
Publisher : Sekretariat Badan Penelitian dan Pengembangan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (675.955 KB) | DOI: 10.21082/ip.v25n1.2016.p41-50

Abstract

An ideal soybean cultivar is the one that achieves the greatest yield consistently across many environments regardless of environmental conditions. The objective of this experiment was to determine the soybean seed yield stability by AMMI method. A total of ten soybean genotypes, which consisted of eight soybean promising lines (G100H/SHRW-60-38, SHRW-60/G100H-73, SHRW-60/G100H-68, SHRW-60/G100H-66, G100H/SHRW-34, SHRW-60/G100H-5, SHRW-60/G100H-70, and SHRW-60/G100H-75) and two soybean check varieties (Kaba and Wilis) was evaluated at six soybean production centers in Yogyakarta, Banyuwangi and West Nusa Tenggara during 2011. The field experimental design for each location was completely randomized design with four replicates. Each genotype was planted on 2.4 m x 4.5 m plot size, 40 cm x 15 cm plant distance, with 2 plants/hill. Stability analysis on seed yield was based on the AMMI method.The combined analysis showed that the genotype and GEI were highly significant for seed yield. The AMMI of the first significant interaction principal component (PC1) accounted for 66.07% of the total variation. Based on AMMI1 biplot, G1 (G100H/SHRW-60-38) and G5 (G100H/SHRW-34) showed higher yield stability. However, the most stable genotype (G5) produced low yield. The other stable genotype (G5) showed highest soybean yield, and therefore it is proposed to be released as a new improved soybean variety.
Keragaman Genetik dan Heritabilitas Beberapa Karakter Agronomi Populasi Galur F4 Padi Beras Hitam Widyayanti, Setyorini; Basunanda, Panjisakti; Mitrowihardjo, Suyadi; Kristamtini, Kristamtini
Jurnal Penelitian Pertanian Tanaman Pangan Vol 1, No 3 (2017): Desember 2017
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.806 KB) | DOI: 10.21082/jpptp.v1n3.2017.p191-199

Abstract

The F4 black rice progenies and their two parents’ varieties were evaluated for their heritability and genetic variability at Padasan, Pakembinangun, Pakem, Sleman, Yogyakarta from May until September 2016. The purpose of this study is to determine genetic diversity, heritability estimates and genetic advance.The genetic materials used in this study consisted of 48 lines of F4 with their parents, local black rice and Situbagendit. The experiment was arranged in a randomized block designed with two replications. Seeds of each F4 progenies and their parents were sown 1 m × 2 m length of plot with 20 cm × 20 cm plant spacing. Genetic parameters estimatesd were calculated for heritability and genetic advance. Plant population of each F4 progenies and their parents were 40 plants in each replication.  The result showed that there were 11 progenies had an early day to maturity potential, 21 F4 progenies had a moderate plant height (60-95 cm), 13 progenies had a potential number of tillers per hill (10.26 to 18.76), 9 F4 progenies had a high number of filled grains per panicle (> 80% filled grains), and 7 F4 progenies had a potential of 1000-grain weight (g). Plant height had the highest coefficient of variability. Days to flowering, days to maturity, and pericarb color had the highest heritability estimates followed by plant height (70.6%). Plant height and pericarb color had the highest genetic advances, namely 21.7% and 49.2%, respectively. In relation to improve agronomic characters, plant height and pericarb color had a highest heritability and genetic advance also a wide genetic variability.
Korelasi Kandungan Antosianin Total dengan Parameter Warna (L*, a*, dan b*) dan Penanda Mikrosatelit pada Beras Hitam Lokal Kristamtini, Kristamtini; Taryono, Taryono; Basunanda, Panjisakti; Murti, Rudi Hari
Jurnal Penelitian Pertanian Tanaman Pangan Vol 1, No 2 (2017): Agustus 2017
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.063 KB) | DOI: 10.21082/jpptp.v1n2.2017.p115-124

Abstract

Black rice is a functional food for its  high anthocyanin contents expressed on purple-black rice color. The study aimed to determine the correlation between: total anthocyanin content with  L *, a *, and b * color variable, total anthocyanin content with color morphology and total anthocyanin content microsatellite markers. Eleven local black rice varieties  from Yogyakarta Assesment Institute of Agriculture Technology collections and two varieties of white rice (Situbagendit and Inpari 6 Jete) were planted on plastic pots in a green house. The tried was arranged   completely randomized design with five replications. At three weeks after planting, the leaf samples were are taken for DNA isolation and further PCR amplifications  using four microsatellite markers (RM 180, RM 220, RM 224 and RM 252). The black rice grain were observed based on color morphology using a Chromameter and analyzed  for then anthocyanin contents. Regression, correlation, and path analysis were conducted to determine the relationship between total anthocyanin content and L* a* b * color variable, color morphology, and microsatellite markers. The results showed that there is correlation between total anthocyanin content  with color parameters (L* = -0,568,  a* = -0,561, b*= 0,844).  Total anthocyanin content is correlation too with color scor of morfology and total   microsatellite markers ((RM 180 =-0,419, RM 220 =0,27, RM 224 =-0,493, RM 252 =0,265) and color scor of morphology  = -0,442. Selection of high-anthocyanin content of rice can use the color variable criterion (L*an  a* low, and  b * high). Microsatellite markers RM 220 and RM 252 can be used as moleculer markers for rice of high anthocyanin content.
Pengujian Stabilitas Membran Sel dan Kandungan Klorofil untuk Evaluasi Toleransi Suhu Tinggi pada Tanaman Kentang Tri Handayani; Panjisakti Basunanda; Rudi Hari Murti; Erri Sofiari
Jurnal Hortikultura Vol 23, No 1 (2013): Maret 2013
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v23n1.2013.p28-35

Abstract

Cekaman suhu tinggi memengaruhi proses fisiologis tanaman dan stabilitas membran sel. Penelitian dilakukan untuk mengevaluasi sifat toleran terhadap cekaman suhu tinggi pada  kentang dengan menguji stabilitas membran sel dan kandungan klorofil. Pengujian terhadap 13 varietas dan tujuh klon kentang dilakukan di Laboratorium Kultur Jaringan dan Laboratorium Fisiologi Tanaman, Balai Penelitian Tanaman Sayuran dari Bulan April sampai Juli 2012. Uji stabilitas membran sel dilakukan melalui pengukuran pelepasan elektrolit akibat kerusakan membran sel oleh suhu tinggi, sedangkan kandungan klorofil diukur menggunakan metode spektrofotometri. Pengujian menggunakan rancangan acak lengkap dengan dua kali ulangan. Hasil penelitian menunjukkan bahwa varietas Cipanas, serta klon CIP 390663.8, CIP 392781.1, CIP 394613.139, dan CIP 395195.7 (planlet dan tanaman di rumah kasa), Merbabu 17 (planlet), serta klon CIP 394614.117 dan varietas Ping 06 (tanaman di rumah kasa) mengalami kerusakan membran sel di bawah 40%. Cekaman suhu tinggi juga menyebabkan penurunan kandungan klorofil.  Penurunan total klorofil yang tinggi (60,20–69,15%) terjadi pada varietas Erika, Manohara, Margahayu, Repita, dan Tenggo, serta klon N.1. Klon CIP 395195.7 dan varietas Ping 06 memiliki kandungan total klorofil yang tinggi pada kondisi suhu tinggi, dengan penurunan total klorofil akibat suhu tinggi yang kecil. Genotip-genotip yang memiliki persentase kerusakan membran sel kecil, kandungan total klorofil yang tinggi, dan penurunan kandungan klorofil yang kecil, diduga memiliki sifat toleran terhadap cekaman suhu tinggi.
Keragaman Genetik Kultivar Padi Beras Hitam Lokal Berdasarkan Penanda Mikrosatelit Kristamtini Kristamtini; Taryono Taryono; Panjisakti Basunanda; Rudi H. Murti
Jurnal AgroBiogen Vol 10, No 2 (2014): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n2.2014.p69-76

Abstract

Indonesia has diverseaccessions of local black rice, which are important sourcesof germplasm. However, some of the local black ricecultivars have different names, leading a need to beidentified to determine their genetic diversity usingmolecular marker. This study aimed to identify geneticdiversity of eleven cultivars of local black rice, collection ofthe Assessment Institute for Agricultural Technology,Yogyakarta and compared them with two white ricevarieties using four microsatellite markers. Detection ofmicrosatellite alleles polymorphism was carried out byvisualization of PCR amplicons by electrophoresis onagarose gel. To estimate their genetic diversity, phylogenetictree and principal coordinate analysis were performed usingbinary data of SSR alleles. The results revealed that totalmarkers enabled to differentiate black rice cultivars asreflected by high value of polymorphic information content(PIC) mean (0.695). This value was consistent with the highgenetic diversity of black rice (genetic diversity index, h =0.283) in comparison with white rice cultivars (h = 0.020).The phylogenetic and main coordinate analyses suggestedthat black rice cultivars genetically differed from white rice.The local black rice cultivars were preferentially groupedbased on their genetic those were distributed in threecoordinates and did not represent their local geographicorigin. Genetic diversity analysis in this study will be usefulas an initial basis for proper identification and selection forappropriate parents to assist breeding program of black ricein Indonesia.
Co-Authors . Suyadi . Suyadi Achmad Syarif Sirojuddin Adie, M Muchlish Arif Meftah Hidayat Atanasius Aditya Hendarto Aziz Purwantoro Dinda Dewanti Erlina Ambarwati Erri Sofiari Gretaryan Wahyu Widiatmiko Hadini, Hamirul Hamirul Hadini Husni Mubarok K. Kristamtini Krisnawati, Ayda Kristamtini Kristamtini Kristamtini Kristamtini Kristamtini Kristamtini Kristamtini Kristamtini Kristamtini, Kristamtini Kristamtini, Kristamtini Kurniawan R. Trijatmiko Ma'sumah Ma'sumah Mariana Susilowati Mirza Bintang Ahimsa Mitrowihardjo, Suyadi Muhammad Habib Widyawan Murti, Rudi Hari Nafarain Haniefan Nasrullah Nasrullah Nasrullah, Nasrullah Nasrullah, nFn Rudi H. Murti Rudi Hari Murti Rudi Hari Murti Rudi Hari Murti Rudi Hari Murti Rudi Hari Murti Sholeh Udin Al Ghifari Siska Permata Siska Permata, Siska Sri Adikadarsih Sri Adikadarsih, Sri Sri Wedhastri Supriyanta Supriyanta Tantri Swandari Taryono, Taryono Taufan Alam Tri Handayani Tri Joko Santoso Wening Enggraini Widya Rachmatika Widyayanti, Setyorini Yefta Pamandungan Yeni Fatmawati